BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,853	C→T	11.3%	A232A (GCC→GCT)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,853	0	C	T	11.3%	576.2 / 33.1	212	A232A (GCC→GCT)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base C (75/104); new base T (4/17); total (82/130)
Fisher's exact test for biased strand distribution p-value = 5.78e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 243 total aligned reads displayed.

GAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACATGCCCCTGGAACT  >  NZ_CP034943/607714‑607991

  gCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcac                                                                                                                                          <  2:516160/140‑1 (MQ=255)

  gCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACGGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcac                                                                                                                                          <  2:1511122/140‑1 (MQ=255)

     cccggAGCTCCCGGCGCCCCCGGAGCCACAGGAGCGACTGGAGCCACCGGGGATACTGGCGAAGTGGGACCCCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacagg                                                                                                                                       <  1:1563134/137‑1 (MQ=12)

      cAGGACCTCCCGGCGCAGCCGGACCAACCGGAGCGACTGGAGCCACAGGGGCTCATGGCGACGTCGGACCCCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGAGGGGAGCCACAGGGGCtactggg                                                                                                                                      <  1:2240667/140‑2 (MQ=14)

       aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggca                                                                                                                                     >  1:588683/1‑140 (MQ=255)

        ggAGCTACCGGGGCAGCCGGCCCAACCGGAGCGACGGGAGCCACAGGCGCTACCGGGGACGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcac                                                                                                                                    <  2:2237140/140‑1 (MQ=18)

           gcgacaggggATGCCGGCCCAGCCGGAGCGACGGGAGCGACAGGGGCTAGTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcacagg                                                                                                                                 <  1:1858526/134‑1 (MQ=17)

           gCTACCGGGGTAGCCGGCCCACCCCGAGCGACTGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcacagg                                                                                                                                 <  1:1527313/140‑1 (MQ=18)

             tACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGag                                                                                                                               <  1:1329685/140‑1 (MQ=255)

              aCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGagc                                                                                                                              >  2:1540483/1‑140 (MQ=255)

               ccGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGggacgtcgga                                                                                                                             >  1:1344266‑M2/1‑130 (MQ=255)

               ccGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGagca                                                                                                                             >  1:345833/1‑140 (MQ=255)

                    gAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCGACAGGggacgtcgcaccagc                                                                                                                        <  2:564583‑M2/140‑16 (MQ=255)

                    gAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACGGGGGCGACTGGggccgtcggaccggc                                                                                                                        <  2:597928‑M2/140‑16 (MQ=255)

                    gAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCGGGGCGACTGGggacgtcgcaccagc                                                                                                                        <  2:2033932‑M2/140‑16 (MQ=255)

                    gAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGggacgtcggaccagc                                                                                                                        <  2:1859678‑M2/140‑16 (MQ=255)

                    gAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGAGACGGGAGCCACTGGGGCGACTGGggacgtcggaccagc                                                                                                                        <  1:768816‑M2/140‑16 (MQ=255)

                                cccGGAGCGCAGGGAGCCACTGGTGCTACTGGTGACGTCGTACCAGACGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                            <  1:1134877/139‑1 (MQ=25)

                                cccGGAGAGACGGGAGCCACAGGGGAACCTGGGGACGCGGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                            <  1:501815/139‑1 (MQ=21)

                                aCCGGAGCGACGGGAGCCACAGGGGCTACTGGTGACGCCGGACCACCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGggacgtcggaccagccggagcgacagg                                                                                                            >  1:1502782‑M2/1‑113 (MQ=255)

                                aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGggacgtcggaccagccggagcgacagg                                                                                                            <  2:1445217‑M2/140‑28 (MQ=255)

                                aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGggacgtcggaccagccggagcgacagg                                                                                                            <  2:1640120‑M2/140‑28 (MQ=255)

                                aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGggacgtcggaccagccggagcgacagg                                                                                                            <  2:54002‑M2/140‑28 (MQ=255)

                                aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACTGGggaagtcgcaccaggcggagcgacagg                                                                                                            <  2:397083‑M2/140‑28 (MQ=255)

                                aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                            <  1:982802/140‑1 (MQ=255)

                                aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                            <  1:2056908/140‑1 (MQ=255)

                                aCCGGAGCGACGCGAGCCATAGGTGTGTCTGGTGTGCTCGGACAAGCCTGATAGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                            <  1:1610269/140‑1 (MQ=21)

                                 ccGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGggacggcggaccagcctgagcgacagga                                                                                                           <  2:2268189‑M2/140‑29 (MQ=255)

                                 ccGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGcgaagtcggaccagccggagcgacagga                                                                                                           <  2:28418‑M2/140‑29 (MQ=255)

                                     cgCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                       <  1:1183909/139‑1 (MQ=255)

                                     aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                       <  1:2194319/140‑1 (MQ=255)

                                     aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                       <  1:1064155/140‑1 (MQ=255)

                                     aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                       <  2:1491780/140‑1 (MQ=255)

                                          cGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTCTCCGAATACGGGtatata                                                                                                  >  1:1975771/1‑140 (MQ=255)

                                            ggAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGtatatata                                                                                                >  1:431785/1‑140 (MQ=255)

                                                     ggggCTACTGGGGAGGTCGGACCAGCCGTAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAg                                                                                       <  1:1454655/140‑1 (MQ=255)

                                                     ggggCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACAGGggaagtcggaccagcctgagcgacatgagctaccggggaagccggaca                                                                                       <  2:2194319‑M2/140‑49 (MQ=255)

                                                      gggCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAgc                                                                                      >  2:1172358/1‑140 (MQ=255)

                                                        gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcac                                                                                                                                    >  1:1096835/1‑92 (MQ=255)

                                                        gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcac                                                                                                                                    <  2:1096835/92‑1 (MQ=255)

                                                        gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAgcgc                                                                                    >  2:1279198/1‑140 (MQ=255)

                                                        gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAgcgc                                                                                    >  2:446920/1‑140 (MQ=255)

                                                        gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAgcgc                                                                                    >  2:2346921/1‑140 (MQ=255)

                                                        gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGATATCCGAATACGGGTATATATATCACTTAAgcgc                                                                                    >  2:1043340/1‑140 (MQ=255)

                                                           aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGagcagc                                                                                                                           <  2:863410/98‑1 (MQ=255)

                                                           aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCa                                                                                 >  2:742108/1‑140 (MQ=255)

                                                                 gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGCCAGGAGCCACGGGAGCCACAGGCACAGCAGCAGCTGGGTTATCCGAATACGGGTAGAGATATAACTTAAGAGCTCAAACGGt                                                                           >  2:1632752/1‑140 (MQ=37)

                                                                 gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACTGGCACAGGAGCAGCTGGTTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                                           <  1:714438/140‑1 (MQ=255)

                                                                 gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                                           >  1:834517/1‑140 (MQ=255)

                                                                 gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                                           >  1:78837/1‑140 (MQ=255)

                                                                 gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                                           <  1:1172358/140‑1 (MQ=255)

                                                                 gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                                           <  1:597928/140‑1 (MQ=255)

                                                                  aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcacagg                                                                                                                                 <  1:876186/85‑1 (MQ=255)

                                                                  aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACCGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTa                                                                          >  1:494042/1‑140 (MQ=255)

                                                                  aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGTATCCGGGTATATATATAACTTAAGCGCTCAAACGGTa                                                                          <  2:582662/140‑1 (MQ=255)

                                                                  aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTa                                                                          >  1:2006655/1‑140 (MQ=255)

                                                                  aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTa                                                                          >  1:1277004/1‑140 (MQ=255)

                                                                  aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTAGCCGAATACGGGGATAGATATAACTTCAGCGCTCAACCGGtc                                                                          >  1:1954425/1‑139 (MQ=37)

                                                                   cGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAg                                                                         >  2:800445/1‑140 (MQ=255)

                                                                   agTCGGACCTCCAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAg                                                                         <  1:1234339/139‑1 (MQ=255)

                                                                    gTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGc                                                                        >  1:1712672/1‑140 (MQ=255)

                                                                    gTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGc                                                                        >  1:2400273/1‑140 (MQ=255)

                                                                      cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                            >  2:2340864/1‑102 (MQ=255)

                                                                      cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                            <  1:2340864/102‑1 (MQ=255)

                                                                         aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcacagg                                                                                                                                 <  1:926281/78‑1 (MQ=255)

                                                                            aGCCGGAGCGACAGGAGCTACCGGGGAAGCCAGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAgg                                                                <  2:257541/140‑1 (MQ=255)

                                                                              ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAAc                                                                              >  2:629904/1‑124 (MQ=255)

                                                                              ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAAc                                                                              <  1:629904/124‑1 (MQ=255)

                                                                              ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCt                                                              >  1:1654550/1‑140 (MQ=255)

                                                                              ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCt                                                              >  1:96662/1‑140 (MQ=255)

                                                                                   gccaCAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                            <  1:561419/86‑1 (MQ=255)

                                                                                           aGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacagg                                                                                                                                       >  2:1563222/1‑54 (MQ=255)

                                                                                            gCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGa                                                <  2:964392/140‑1 (MQ=255)

                                                                                                 cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACGGGAGCCCCAGGCCCAGGCGCAGCTCGGTTATCCGAATCGGGGTCGCGATATAACGTAGCCGCTCAAGCGGTAGCTCGAGAGGGTGAGGTCATAGGTGATTCac                                           >  1:2150500/1‑139 (MQ=16)

                                                                                                 cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCaa                                           <  1:446920/140‑1 (MQ=255)

                                                                                                 cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCaa                                           <  1:1279798/140‑1 (MQ=255)

                                                                                                 cGGGGAAGACGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACCGGTATATATATAACGTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCaa                                           <  2:1954898/140‑1 (MQ=255)

                                                                                                          cGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATaa                                  <  1:324075/140‑1 (MQ=255)

                                                                                                           ggACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAac                                 <  2:2150500/140‑1 (MQ=255)

                                                                                                           ggACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAac                                 <  1:2111862/140‑1 (MQ=255)

                                                                                                           ggACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAac                                 <  2:345833/140‑1 (MQ=255)

                                                                                                             aCCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAacca                               >  2:590841/1‑140 (MQ=255)

                                                                                                                 aCCGGAGCGACGGGAGCCACGGGGGCGACAGGCACAGTAGCAGCTGGCTTATCCGAAGCCGGGTCGATATAAAACTTAAGAGCAGCAGCGGTAGGAATAGAGGCTGATGTCATTTAGGTGTCAACAGGGATAACCAcccc                           >  2:897911/1‑140 (MQ=255)

                                                                                                                 aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc                           <  1:1142347/140‑1 (MQ=255)

                                                                                                                 aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc                           <  1:897911/140‑1 (MQ=255)

                                                                                                                 aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc                           <  2:719679/140‑1 (MQ=255)

                                                                                                                 aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc                           <  1:2033932/140‑1 (MQ=255)

                                                                                                                 aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc                           <  1:54002/140‑1 (MQ=255)

                                                                                                                 aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc                           <  2:1131481/140‑1 (MQ=255)

                                                                                                                 aCCGGAGCGACAGGAGCCAAGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc                           <  2:1265645/140‑1 (MQ=255)

                                                                                                                       gccaCAGGAGCCACGGGAGCCACCGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTAGATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGaag                     <  1:300873/137‑2 (MQ=255)

                                                                                                                          aCAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTac                  >  1:141069/1‑140 (MQ=255)

                                                                                                                          aCAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTac                  >  1:1023692/1‑140 (MQ=255)

                                                                                                                             ggAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTAcaca               <  1:751087/140‑1 (MQ=255)

                                                                                                                              gAGCCACGGGAGCCAGAGGCACAGGAGGAGCTGGGTTATCCGAATTGGGGTATATATATATCTTATGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGATTTACACAt              <  2:2098390/140‑1 (MQ=255)

                                                                                                                              gAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACAt              <  1:2020068/140‑1 (MQ=255)

                                                                                                                              gAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACAt              <  2:2125648/140‑1 (MQ=255)

                                                                                                                              gAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACAt              <  1:38710/140‑1 (MQ=255)

                                                                                                                                gCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCAtt                                                     <  1:1901686/99‑1 (MQ=255)

                                                                                                                                gCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCAtt                                                     >  2:1901686/1‑99 (MQ=255)

GAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACATGCCCCTGGAACT  >  NZ_CP034943/607714‑607991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`