BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,856	A→T	12.0%	T233T (ACA→ACT)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,856	0	A	T	12.0%	638.9 / 33.3	213	T233T (ACA→ACT)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base A (77/105); new base T (6/23); total (83/130)
Fisher's exact test for biased strand distribution p-value = 3.92e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 241 total aligned reads displayed.

ACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACATGCCCCTGGAACTTC  >  NZ_CP034943/607719‑607993

cccggAGCTCCCGGCGCCCCCGGAGCCACAGGAGCGACTGGAGCCACCGGGGATACTGGCGAAGTGGGACCCCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacagg                                                                                                                                         <  1:1563134/137‑1 (MQ=12)

  aGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGCCAGGAGCCACGGGAGCaacaggca                                                                                                                                       >  1:1711496/1‑140 (MQ=255)

  aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggca                                                                                                                                       >  1:588683/1‑140 (MQ=255)

   ggAGCTACCGGGGCAGCCGGCCCAACCGGAGCGACGGGAGCCACAGGCGCTACCGGGGACGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcac                                                                                                                                      <  2:2237140/140‑1 (MQ=18)

     cgcTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGggacg                                                                                                                                    >  1:1988713‑M2/2‑135 (MQ=255)

      gcgacaggggATGCCGGCCCAGCCGGAGCGACGGGAGCGACAGGGGCTAGTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcacagg                                                                                                                                   <  1:1858526/134‑1 (MQ=17)

      gCTACCGGGGTAGCCGGCCCACCCCGAGCGACTGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcacagg                                                                                                                                   <  1:1527313/140‑1 (MQ=18)

        tACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGag                                                                                                                                 <  1:1329685/140‑1 (MQ=255)

          ccGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGggacgtcgga                                                                                                                               >  1:1344266‑M2/1‑130 (MQ=255)

           cGGGGAAGCCGGACCAACCGGCGCGACGGGCGCCACAGGGGCTACTGGGGCCCTCGGCCGCGCGGGAGCGTCAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGagcag                                                                                                                              <  2:1642358/140‑1 (MQ=18)

           cGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCCACGGGGGCtactgggacagtcggac                                                                                                                              >  2:377563/1‑134 (MQ=17)

               gAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCGGGGCGACTGGggacgtcgcaccagc                                                                                                                          <  2:2033932‑M2/140‑16 (MQ=255)

               gAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGggacgtcggaccagc                                                                                                                          <  2:1859678‑M2/140‑16 (MQ=255)

               gAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGgcacgtaggaccggc                                                                                                                          <  2:1563962‑M2/140‑16 (MQ=255)

               gAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGAGACGGGAGCCACTGGGGCGACTGGggacgtcggaccagc                                                                                                                          <  1:768816‑M2/140‑16 (MQ=255)

                    cGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTAt                                                                                                                     >  2:586731/1‑140 (MQ=255)

                           gccGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                              <  2:580277/139‑1 (MQ=255)

                           ccgggAGCGACGGGAGCCACCGGGGCTACTGGCGACGCCGGACCAGCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                              <  2:1541476/137‑1 (MQ=21)

                           cccGGAGCGCAGGGAGCCACTGGTGCTACTGGTGACGTCGTACCAGACGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                              <  1:1134877/139‑1 (MQ=25)

                           aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                              <  1:1399789/140‑1 (MQ=255)

                           aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                              <  1:2056908/140‑1 (MQ=255)

                            ccGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGggacgtcggaccagccggagcgacagga                                                                                                             <  1:516160‑M2/140‑29 (MQ=255)

                            ccGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGcgaagtcggaccagccggagcgacagga                                                                                                             <  2:28418‑M2/140‑29 (MQ=255)

                            ccGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCCACAGGGGCGACTGGggacgtcgcaccagccggagcgacagca                                                                                                             <  2:2111862‑M2/140‑29 (MQ=255)

                                cgCGTCGGGAGCCTCAGGGGGTACAGGGGACGTCGGACGAGACGGAGAGAGAGGATGTACCGGGGAAGCCGGACGATCCTGAGCGACATGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                         <  2:1950356/139‑1 (MQ=255)

                                cgCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                         <  1:1183909/139‑1 (MQ=255)

                                aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                         <  2:1491780/140‑1 (MQ=255)

                                aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                         <  1:223312/140‑1 (MQ=255)

                                aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                         <  1:2194319/140‑1 (MQ=255)

                                aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                         <  1:1064155/140‑1 (MQ=255)

                                     cGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTCTCCGAATACGGGtatata                                                                                                    >  1:1975771/1‑140 (MQ=255)

                                                ggggCTACTGGGGAGGTCGGACCAGCCGTAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAg                                                                                         <  1:1454655/140‑1 (MQ=255)

                                                ggggCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACAGGggaagtcggaccagcctgagcgacatgagctaccggggaagccggaca                                                                                         <  2:2194319‑M2/140‑49 (MQ=255)

                                                 gggCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAgc                                                                                        >  2:1172358/1‑140 (MQ=255)

                                                   gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcac                                                                                                                                      <  2:1096835/92‑1 (MQ=255)

                                                   gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcac                                                                                                                                      >  1:1096835/1‑92 (MQ=255)

                                                   gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAgcgc                                                                                      >  2:446920/1‑140 (MQ=255)

                                                   gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAgcgc                                                                                      >  2:1279198/1‑140 (MQ=255)

                                                   gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGATATCCGAATACGGGTATATATATCACTTAAgcgc                                                                                      >  2:1043340/1‑140 (MQ=255)

                                                      aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGagcagc                                                                                                                             <  2:863410/98‑1 (MQ=255)

                                                      aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCa                                                                                   >  2:1955002/1‑140 (MQ=255)

                                                      aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCa                                                                                   >  2:742108/1‑140 (MQ=255)

                                                       cTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTggg                                                                                                                         <  1:268777/101‑1 (MQ=255)

                                                            gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGCCAGGAGCCACGGGAGCCACAGGCACAGCAGCAGCTGGGTTATCCGAATACGGGTAGAGATATAACTTAAGAGCTCAAACGGt                                                                             >  2:1632752/1‑140 (MQ=37)

                                                            gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACTGGCACAGGAGCAGCTGGTTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                                             <  1:714438/140‑1 (MQ=255)

                                                            gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                                             >  2:598817/1‑140 (MQ=255)

                                                            gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                                             >  1:977382/1‑140 (MQ=255)

                                                            gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                                             >  1:834517/1‑140 (MQ=255)

                                                            gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                                             >  1:78837/1‑140 (MQ=255)

                                                            gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                                             <  1:1172358/140‑1 (MQ=255)

                                                            gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                                             >  1:44667/1‑140 (MQ=255)

                                                             aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcacagg                                                                                                                                   <  1:876186/85‑1 (MQ=255)

                                                             aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTa                                                                            <  1:1603940/140‑1 (MQ=255)

                                                             aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTa                                                                            >  1:1277004/1‑140 (MQ=255)

                                                             aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTAGCCGAATACGGGGATAGATATAACTTCAGCGCTCAACCGGtc                                                                            >  1:1954425/1‑139 (MQ=37)

                                                              cGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAg                                                                           >  2:800445/1‑140 (MQ=255)

                                                               gTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGc                                                                          <  1:500773/140‑1 (MQ=255)

                                                                 cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                              <  1:2340864/102‑1 (MQ=255)

                                                                 cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                              >  2:2340864/1‑102 (MQ=255)

                                                                    aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcacagg                                                                                                                                   <  1:926281/78‑1 (MQ=255)

                                                                         ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAAc                                                                                <  1:629904/124‑1 (MQ=255)

                                                                         ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCt                                                                >  1:1654550/1‑140 (MQ=255)

                                                                         ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCt                                                                >  1:96662/1‑140 (MQ=255)

                                                                              gccaCAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                              >  2:561419/4‑89 (MQ=255)

                                                                                  cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTAGAGAGAAAACTTAAGCGCTCAAACGGAAGCTCGAGAGGCTGAGGTCAta                                                       >  2:323931/1‑139 (MQ=37)

                                                                                       gCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGa                                                  <  2:964392/140‑1 (MQ=255)

                                                                                          aCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCCACAGGCACAGGAGCAGCAGGGTTATCCGAATAAGGTTCGATCTATCACTTAAGCGCACAAACGCTCGCTCTAGAGGCTGATGTCAGTTTTGATTc                                               >  1:1867746/1‑140 (MQ=25)

                                                                                            cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCaa                                             <  1:1279798/140‑1 (MQ=255)

                                                                                             ggaccaGCCGGACGAACGGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAAc                                            <  2:2302165/135‑1 (MQ=255)

                                                                                                     cGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATaa                                    <  1:323931/140‑1 (MQ=255)

                                                                                                     cGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATaa                                    <  1:552917/140‑1 (MQ=255)

                                                                                                      ggACCAACCGGATCGACAGTAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATTTCATTTTTGATTCAACAGGGATAac                                   <  1:33507/140‑1 (MQ=255)

                                                                                                      ggACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACATGAGCAGCTGGGTTATCCGAATAAGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAac                                   <  1:2368703/140‑1 (MQ=255)

                                                                                                      ggACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAac                                   <  1:2106581/140‑1 (MQ=255)

                                                                                                      ggACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAac                                   <  1:2032344/140‑1 (MQ=255)

                                                                                                      ggACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAac                                   <  1:1233347/140‑1 (MQ=255)

                                                                                                        aCCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAacca                                 >  2:591107/1‑140 (MQ=255)

                                                                                                            aCCGGAGCGACGGGAGCCACGGGGGCGACAGGCACAGTAGCAGCTGGCTTATCCGAAGCCGGGTCGATATAAAACTTAAGAGCAGCAGCGGTAGGAATAGAGGCTGATGTCATTTAGGTGTCAACAGGGATAACCAcccc                             >  2:897911/1‑140 (MQ=255)

                                                                                                            aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc                             <  1:397083/140‑1 (MQ=255)

                                                                                                            aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc                             <  2:215631/140‑1 (MQ=255)

                                                                                                            aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc                             <  1:1142347/140‑1 (MQ=255)

                                                                                                            aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc                             <  2:1131481/140‑1 (MQ=255)

                                                                                                            aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc                             <  1:897911/140‑1 (MQ=255)

                                                                                                            aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc                             <  2:719679/140‑1 (MQ=255)

                                                                                                            aCCGGAGCGACAGGAGCCAAGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc                             <  2:1265645/140‑1 (MQ=255)

                                                                                                             ccGGAGCGACAGGAGCCACGGGAGCCCCAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCa                            >  2:243864/1‑140 (MQ=255)

                                                                                                             ccGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGAGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCa                            >  2:1435435/1‑140 (MQ=255)

                                                                                                                     aCAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTac                    >  1:1023692/1‑140 (MQ=255)

                                                                                                                        ggAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTAcaca                 <  1:1500290/140‑1 (MQ=255)

                                                                                                                        ggAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTAcaca                 <  1:59208/140‑1 (MQ=255)

                                                                                                                        ggAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTAcaca                 <  1:751087/140‑1 (MQ=255)

                                                                                                                         gAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACAt                <  2:2125648/140‑1 (MQ=255)

                                                                                                                         gAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACAt                <  1:2020068/140‑1 (MQ=255)

                                                                                                                         gAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACAt                <  1:38710/140‑1 (MQ=255)

                                                                                                                                gggAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTAcaca                 <  1:2119647/132‑1 (MQ=255)

                                                                                                                                   aGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACATGCCCCTGGaa      >  2:524247/1‑140 (MQ=255)

                                                                                                                                   aGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACATGCCCCTGGaa      >  2:1527267/1‑140 (MQ=255)

                                                                                                                                     ccACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACATGCCCCTGGACCt    >  1:460301/1‑140 (MQ=255)

                                                                                                                                       ccaggctcagtAGCAGCTGGGTTATCCTTATACGTGTATATATATAACTTAAGCGATCAATCGGTAGCTATAGAGGATTATGTCTTTTTTGATTCACCAGGGCTAACCAGCCCAGGTATTACACATGCCCCTGGAACTtc  <  2:65623/139‑1 (MQ=255)

                                                                                                                                       acaggcacaggAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACATGCCCCTGGAACTtc  <  2:2403953/140‑1 (MQ=255)

ACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACATGCCCCTGGAACTTC  >  NZ_CP034943/607719‑607993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`