BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	751,373	A→T	5.7%	M189L (ATG→TTG)	EO946_RS03865 →	YesL family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	751,373	0	A	T	5.7%	595.6 / 20.1	174	M189L (ATG→TTG)	EO946_RS03865	YesL family protein
Reads supporting (aligned to +/- strand): ref base A (91/73); new base T (5/5); total (96/78)
Fisher's exact test for biased strand distribution p-value = 7.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01

Only 100 of 175 total aligned reads displayed.

CAAGCGCTGCTGTTTGGCGCCATGCGGCCGCTGACGACCGGCTGTATGCTGATCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAGAC  >  NZ_CP034943/751238‑751507

      ctgctgTTTGGCGCCATGCGGCCGCTGACGACCGGCTGTATGCTGATCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGttttt                                                                                                                              <  1:17645/140‑1 (MQ=255)

        gctgTTTGGCGCCATGCGGCCGCTGACGACCGGCTGTATGCTGATCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGGTAGTGATGATGTTTTTTg                                                                                                                            >  1:1968878/1‑140 (MQ=255)

        gctgTTTGGCGCCATGCGGCCGCTGACGACCGGCTGTATGCTGATCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTg                                                                                                                            >  1:333445/1‑140 (MQ=255)

                      tgagaccGCTGACAACTGGCTGTATGCTGATCGGGTGCGGATTTGTCCTTTATTTGCTGTATACGCTTCCTGGCCTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTTGTGTTGATGTTTTTTGCTCTTAGGGGAttt                                                                                                              >  2:786549/6‑140 (MQ=255)

                         ggCCGCTGACGACCGGCTGTATGCTGATCGGGTGCGGATTTGTCCTGTACTTGCTGTATACGCTGCCGGGATTGATCCCAATTTACGGCCCGTGTTTATGTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCaa                                                                                                           <  2:1411976/140‑1 (MQ=255)

                                  cgacCGGCTGTATGCTGATCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAg                                                                                                  >  2:1671537/1‑140 (MQ=255)

                                        gCTGTATGCTGATCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAg                                                                                            <  2:1734861/140‑1 (MQ=255)

                                                   aTCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGAc                                                                                                      <  2:1389052/119‑1 (MQ=255)

                                                   aTCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGAc                                                                                                      <  2:1481121/119‑1 (MQ=255)

                                                   aTCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGAc                                                                                                      >  1:1481121/1‑119 (MQ=255)

                                                   aTCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAg                                                                                            <  2:703475/129‑1 (MQ=255)

                                                   aTCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAg                                                                                            <  2:2137568/129‑1 (MQ=255)

                                                   aTCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGc                                                                                 >  2:1657809/1‑140 (MQ=255)

                                                   aTCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGc                                                                                 >  1:73697/1‑140 (MQ=255)

                                                   aTCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGc                                                                                 >  2:1220572/1‑140 (MQ=255)

                                                   aTCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGc                                                                                 >  1:1073669/1‑140 (MQ=255)

                                                   aTCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGc                                                                                 >  2:2277926/1‑140 (MQ=255)

                                                   aTCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGc                                                                                 >  2:522722/1‑140 (MQ=255)

                                                   aTCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGc                                                                                 >  2:893873/1‑140 (MQ=255)

                                                   aTCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTAAGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGc                                                                                 >  1:2371250/1‑140 (MQ=255)

                                                        gTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGtttttt                                                                                                                             >  2:1307790/1‑91 (MQ=255)

                                                        gTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGtt                                                                            >  2:1643682/1‑140 (MQ=255)

                                                                  gTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCTTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACaaaaaaa                                                                  >  1:1153124/1‑140 (MQ=255)

                                                                  gTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCGGGTTAACaaaaaaa                                                                  >  2:680459/1‑140 (MQ=255)

                                                                        tACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACaa                                                            >  1:656694/1‑140 (MQ=255)

                                                                             gCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATg                                                       >  1:1021859/1‑140 (MQ=255)

                                                                             gCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATg                                                       >  1:644683/1‑140 (MQ=255)

                                                                             gCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATg                                                       >  1:1021636/1‑140 (MQ=255)

                                                                             gCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATg                                                       <  1:1441983/140‑1 (MQ=255)

                                                                             gCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTGGCTCACAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATg                                                       <  2:1610054/140‑1 (MQ=255)

                                                                              cTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGc                                                      <  1:1718839/140‑1 (MQ=255)

                                                                              cTGTATACGCTGCCGGGATTGATCACATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCATGTTAACAAAAAAATGACAATCATGc                                                      <  1:2108749/140‑1 (MQ=255)

                                                                               tGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCa                                                     <  2:2428775/140‑1 (MQ=255)

                                                                               tGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCa                                                     <  2:1052134/140‑1 (MQ=255)

                                                                               tGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCa                                                     <  2:1677469/140‑1 (MQ=255)

                                                                               tGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCa                                                     <  2:1781512/140‑1 (MQ=255)

                                                                                  ataCGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCaaaa                                                  <  2:182723/140‑1 (MQ=255)

                                                                                       cTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTa                                             <  2:346755/140‑1 (MQ=255)

                                                                                        tGCCGGGATTGATCCCATTTTACGGCGCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACCATCATGCAAAAAAGtcc                                            >  2:1512422/1‑138 (MQ=255)

                                                                                        tGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCaaaa                                                  <  2:825513/134‑1 (MQ=255)

                                                                                        tGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCaaaa                                                  >  1:825513/1‑134 (MQ=255)

                                                                                        tGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTAc                                            >  1:441397/1‑140 (MQ=255)

                                                                                        tGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTAc                                            >  1:1695630/1‑140 (MQ=255)

                                                                                        tGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTAc                                            >  1:1376788/1‑140 (MQ=255)

                                                                                        tGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTAc                                            >  1:39946/1‑140 (MQ=255)

                                                                                        tGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTAc                                            >  1:1917008/1‑140 (MQ=255)

                                                                                        tGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTAc                                            >  1:2156430/1‑140 (MQ=255)

                                                                                        tGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTAc                                            >  1:1359917/1‑140 (MQ=255)

                                                                                        tGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTAc                                            >  1:1625754/1‑140 (MQ=255)

                                                                                         gCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACa                                           >  1:1824956/1‑140 (MQ=255)

                                                                                         gCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACa                                           >  1:367725/1‑140 (MQ=255)

                                                                                         gCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACa                                           <  2:1785167/140‑1 (MQ=255)

                                                                                         gCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACa                                           <  2:2371250/140‑1 (MQ=255)

                                                                                         gCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACa                                           >  2:946142/1‑140 (MQ=255)

                                                                                         gCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACa                                           >  2:837273/1‑140 (MQ=255)

                                                                                          ccGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAATATGACAATCATGCAAAAATGTACAg                                          >  1:122157/1‑140 (MQ=255)

                                                                                           ctggcctGATCCCATTTTACGGCCCGTGTTTATTTGGGCTTGTGTTGATGTTTTTTGCTCTTAGGGGATTTCAAAAGACGGAGGCCCAGCACCATCAAGCCGGGTAACAAAAAAATGACAAATAAGCAAAAAAGTGCAgt                                         >  1:1302777/7‑140 (MQ=255)

                                                                                           ctggcctGATCCCATTTTACGGCCCGTGTTTATTTGGGCTTGTGTTGATGTTTTTTGCTCTTAGGGGATTTCAAAAGACGGAGGCCCAGCACCATCAAGCCGGGTAACAAAAAAATGACAAATAAGCAAAAAAGTGCAgt                                         >  1:252917/7‑140 (MQ=255)

                                                                                               aTTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCAGGCAAAAAAGTACAGTGTcc                                     >  2:1702564/1‑140 (MQ=255)

                                                                                                  gATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGaa                                  >  1:408920/1‑140 (MQ=255)

                                                                                                  gATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGaa                                  >  1:2128370/1‑140 (MQ=255)

                                                                                                  gATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGaa                                  >  2:1576731/1‑140 (MQ=255)

                                                                                                  gATCCCATTTTACGGCCCGGGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAATTGACAATCATGCAAAACAGTACAGTGTCCgga                                  >  1:1917473/1‑138 (MQ=255)

                                                                                                             aCGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATg                       >  2:45137/1‑140 (MQ=255)

                                                                                                              cGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGc                      >  2:1603978/1‑140 (MQ=255)

                                                                                                                 cccGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGc                            <  1:1516484/131‑1 (MQ=255)

                                                                                                                  cggtgtTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCaaaa                  <  2:2195860/138‑1 (MQ=255)

                                                                                                                  ccGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCaaaa                  <  1:937775/140‑1 (MQ=255)

                                                                                                                  ccGTGTTTATTTGGGCTAGTGATGATGTTTTGTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATCACAATCATGCAACAAAGTACAGTGTCCGAAAAAGGCAGATGCaaaa                  <  2:63454/140‑1 (MQ=255)

                                                                                                                   cGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCaaaaa                 <  1:1581398/140‑1 (MQ=255)

                                                                                                                   cGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCaaaaa                 >  1:370208/1‑140 (MQ=255)

                                                                                                                   cGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCaaaaa                 <  1:1189652/140‑1 (MQ=255)

                                                                                                                   cGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCaaaaa                 <  1:1910947/140‑1 (MQ=255)

                                                                                                                    gtgtTTATTTGGGCTAGTGATGATGTTTTTTGCTATCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCaaaaaa                >  1:162397/1‑140 (MQ=255)

                                                                                                                       tttATTTGGGCTTGTGTTGATGTTTTTTGCTCTTAGGGGATTTCAAAAGACGGAGGCCCAGCACCATCAAGCCGGGTAACAAAAAAATGACAAATAAGCAAAAAAGTGCAGTGTCCGAAATAGGCAGATGCAAAAAAgtg             <  2:1302776/140‑1 (MQ=255)

                                                                                                                       tttATTTGGGCTTGTGTTGATGTTTTTTGCTCTTAGGGGATTTCAAAAGACGGAGGCCCAGCACCATCAAGCCGGGTAACAAAAAAATGACAAATAAGCAAAAAAGTGCAGTGTCCGAAATAGGCAGATGCAAAAAAgtg             <  2:63457/140‑1 (MQ=255)

                                                                                                                       tttATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAgtg             <  2:761458/140‑1 (MQ=255)

                                                                                                                       tttATGTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATGTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAgtg             <  1:1933676/140‑1 (MQ=255)

                                                                                                                             tGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAAttt       >  1:2414410/1‑139 (MQ=255)

                                                                                                                               ggCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCa                                                     <  2:1466464/92‑1 (MQ=255)

                                                                                                                               ggCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGaa     >  2:149836/1‑140 (MQ=255)

                                                                                                                                gCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGacg    <  1:676803/140‑3 (MQ=255)

                                                                                                                                gCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAg    <  2:117748/140‑1 (MQ=255)

                                                                                                                                gCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAg    <  1:421638/140‑1 (MQ=255)

                                                                                                                                gCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAg    <  1:676709/140‑1 (MQ=255)

                                                                                                                                gCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAg    <  1:2253256/140‑1 (MQ=255)

                                                                                                                                gCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAg    <  2:656694/140‑1 (MQ=255)

                                                                                                                                gCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAg    <  2:1140492/140‑1 (MQ=255)

                                                                                                                                gCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAg    <  1:1315807/140‑1 (MQ=255)

                                                                                                                                gCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAg    <  2:413873/140‑1 (MQ=255)

                                                                                                                                gCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAg    <  2:331156/140‑1 (MQ=255)

                                                                                                                                gCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAg    <  1:1759378/140‑1 (MQ=255)

                                                                                                                                 cTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGACGa   >  2:1963157/1‑140 (MQ=255)

                                                                                                                                 cTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAGa   >  2:307761/1‑140 (MQ=255)

                                                                                                                                 cTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAGa   <  1:1188618/140‑1 (MQ=255)

                                                                                                                                 cTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAACTGTAATTGAAGa   >  2:1353822/1‑140 (MQ=255)

                                                                                                                                  tAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAGAc  <  1:946142/140‑1 (MQ=255)

                                                                                                                                  tAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAGAc  <  2:2042364/140‑1 (MQ=255)

                                                                                                                                    gTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATg                                                       >  2:666056/1‑85 (MQ=255)

                                                                                                                                    gTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATg                                                       <  1:164786/85‑1 (MQ=255)

CAAGCGCTGCTGTTTGGCGCCATGCGGCCGCTGACGACCGGCTGTATGCTGATCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAGAC  >  NZ_CP034943/751238‑751507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`