BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	751,393	A→G	5.2%	R195R (AGA→AGG)	EO946_RS03865 →	YesL family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	751,393	0	A	G	5.2%	619.4 / 14.6	190	R195R (AGA→AGG)	EO946_RS03865	YesL family protein
Reads supporting (aligned to +/- strand): ref base A (99/81); new base G (5/5); total (104/86)
Fisher's exact test for biased strand distribution p-value = 7.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 190 total aligned reads displayed.

GGCCGCTGACGACCGGCTGTATGCTGATCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAGACAGCTCACACAGCGCATAAAAT  >  NZ_CP034943/751263‑751528

ggCCGCTGACGACCGGCTGTATGCTGATCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCaa                                                                                                                                <  2:784315/140‑1 (MQ=255)

ggCCGCTGACGACCGGCTGTATGCTGATCGGGTGCGGATTTGTCCTGTACTTGCTGTATACGCTGCCGGGATTGATCCCAATTTACGGCCCGTGTTTATGTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCaa                                                                                                                                <  2:1411976/140‑1 (MQ=255)

ggCCGCTGACGACCGGATGTATGCTGATCGTGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCaa                                                                                                                                <  2:1617826/140‑1 (MQ=255)

       gacaacTGGCTGTATGCTGATCGGGTGCGGATTTGTCCTTTATTTGCTGTATACGCTTCCTGGCCTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTTGTGTTGATGTTTTTTGCTCTTAGGGGATTTCAAAAGACgg                                                                                                                         <  1:786549/140‑1 (MQ=255)

         cgacCGGCTGTATGCTGATCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAg                                                                                                                       >  2:1792340/1‑140 (MQ=255)

          gacCGGCTGTATGCTGATCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAgg                                                                                                                      <  1:2289825/140‑1 (MQ=255)

            ccGGCTGTATGCTGATCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCt                                                                                                                    >  1:331156/1‑140 (MQ=255)

               gCTGTATGCTGATCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAg                                                                                                                 <  2:1734861/140‑1 (MQ=255)

                          aTCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGAc                                                                                                                           <  2:1389052/119‑1 (MQ=255)

                          aTCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGAc                                                                                                                           <  2:1481121/119‑1 (MQ=255)

                          aTCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAg                                                                                                                 >  1:2137568/1‑129 (MQ=255)

                          aTCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAg                                                                                                                 <  2:703475/129‑1 (MQ=255)

                          aTCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAg                                                                                                                 <  2:2137568/129‑1 (MQ=255)

                          aTCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGc                                                                                                      >  2:2277926/1‑140 (MQ=255)

                          aTCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGc                                                                                                      >  2:1669223/1‑140 (MQ=255)

                          aTCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGc                                                                                                      >  2:1849177/1‑140 (MQ=255)

                          aTCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGc                                                                                                      >  2:2188942/1‑140 (MQ=255)

                          aTCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGc                                                                                                      >  2:309702/1‑140 (MQ=255)

                          aTCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGc                                                                                                      >  2:522722/1‑140 (MQ=255)

                          aTCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGc                                                                                                      >  1:73697/1‑140 (MQ=255)

                          aTCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGc                                                                                                      >  2:1220572/1‑140 (MQ=255)

                                         gTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCGGGTTAACaaaaaaa                                                                                       >  2:680459/1‑140 (MQ=255)

                                         gTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACaaaaaaa                                                                                       >  1:387942/1‑140 (MQ=255)

                                               tACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACaa                                                                                 >  1:407878/1‑140 (MQ=255)

                                               tACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACaa                                                                                 >  1:413873/1‑140 (MQ=255)

                                               tACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACaa                                                                                 >  1:656694/1‑140 (MQ=255)

                                                    gCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATg                                                                            <  2:1102749/140‑1 (MQ=255)

                                                    gCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATg                                                                            >  1:644683/1‑140 (MQ=255)

                                                    gCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATg                                                                            >  1:1021636/1‑140 (MQ=255)

                                                    gCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATg                                                                            <  1:1441983/140‑1 (MQ=255)

                                                     cTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGc                                                                           <  1:1718712/140‑1 (MQ=255)

                                                     cTGTATACGCTGCCGGGATTGATCACATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCATGTTAACAAAAAAATGACAATCATGc                                                                           <  1:2108749/140‑1 (MQ=255)

                                                      tGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCa                                                                          <  2:1677469/140‑1 (MQ=255)

                                                      tGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCa                                                                          <  2:1052134/140‑1 (MQ=255)

                                                      tGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCa                                                                          <  2:1781512/140‑1 (MQ=255)

                                                      tGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCa                                                                          <  2:1163296/140‑1 (MQ=255)

                                                         ataCGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCaaaa                                                                       <  2:182723/140‑1 (MQ=255)

                                                           aCGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAAGAAAAAAATGACAATCATGCaaaaaa                                                                     >  2:741863/1‑140 (MQ=255)

                                                               tGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTAc                                                                 >  1:245512/1‑140 (MQ=255)

                                                               tGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTAc                                                                 >  1:1625754/1‑140 (MQ=255)

                                                               tGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTAc                                                                 >  1:441397/1‑140 (MQ=255)

                                                               tGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTAc                                                                 >  1:1376788/1‑140 (MQ=255)

                                                               tGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTAc                                                                 >  1:1359917/1‑140 (MQ=255)

                                                               tGCCGGGATTGATCCCATTTTAAGTACAGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTACCAAAAAAATGAGAATCATGCAAAAAAGAAc                                                                 >  1:1868881/1‑140 (MQ=255)

                                                                gCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACa                                                                >  2:946142/1‑140 (MQ=255)

                                                                gCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACa                                                                >  2:1315807/1‑140 (MQ=255)

                                                                gCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACa                                                                >  2:837273/1‑140 (MQ=255)

                                                                gCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACa                                                                >  2:466141/1‑140 (MQ=255)

                                                                gCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACa                                                                <  2:2371250/140‑1 (MQ=255)

                                                                gCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACa                                                                >  1:367725/1‑140 (MQ=255)

                                                                 ccGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAATATGACAATCATGCAAAAATGTACAg                                                               >  1:122157/1‑140 (MQ=255)

                                                                  ctggcctGATCCCATTTTACGGCCCGTGTTTATTTGGGCTTGTGTTGATGTTTTTTGCTCTTAGGGGATTTCAAAAGACGGAGGCCCAGCACCATCAAGCCGGGTAACAAAAAAATGACAAATAAGCAAAAAAGTGCAgt                                                              >  1:1302777/7‑140 (MQ=255)

                                                                    ggATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAgtgt                                                            <  1:1069728/140‑1 (MQ=255)

                                                                      aTTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCAGGCAAAAAAGTACAGTGTcc                                                          >  2:1702564/1‑140 (MQ=255)

                                                                         gATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCCAAAAAGTACAGTGTCCGaa                                                       >  1:1915910/1‑140 (MQ=255)

                                                                         gATCCCATTTTACGGCCCGGGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAATTGACAATCATGCAAAACAGTACAGTGTCCgga                                                       >  1:1917473/1‑138 (MQ=255)

                                                                                     cGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGc                                           >  2:1603978/1‑140 (MQ=255)

                                                                                        cccGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGc                                                 <  1:1516484/131‑1 (MQ=255)

                                                                                        cccGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGc                                                 >  2:1516484/1‑131 (MQ=255)

                                                                                        cccGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGCCAGATGCaaa                                        >  2:635641/1‑140 (MQ=255)

                                                                                         ccGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCaaaa                                       <  2:1598718/140‑1 (MQ=255)

                                                                                         ccGTGTTTATTTGGGCTAGTGATGATGTTTTGTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATCACAATCATGCAACAAAGTACAGTGTCCGAAAAAGGCAGATGCaaaa                                       <  2:63454/140‑1 (MQ=255)

                                                                                          cGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCaaaaa                                      <  1:1581398/140‑1 (MQ=255)

                                                                                          cGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTAAAGTGTCCGAAAAAGGCAGATGCaaaaa                                      >  1:1750294/1‑140 (MQ=255)

                                                                                           gtgtTTATTTGGGCTAGTGATGATGTTTTTTGCTATCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCaaaaaa                                     >  1:162397/1‑140 (MQ=255)

                                                                                              tttATTTGGGCTTGTGTTGATGTTTTTTGCTCTTAGGGGATTTCAAAAGACGGAGGCCCAGCACCATCAAGCCGGGTAACAAAAAAATGACAAATAAGCAAAAAAGTGCAGTGTCCGAAATAGGCAGATGCAAAAAAgtg                                  <  2:1302776/140‑1 (MQ=255)

                                                                                              tttATGTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATGTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAgtg                                  <  1:1933676/140‑1 (MQ=255)

                                                                                                    tGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAAttt                            >  1:2414410/1‑139 (MQ=255)

                                                                                                      ggCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCa                                                                          >  1:1466464/1‑92 (MQ=255)

                                                                                                      ggCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCa                                                                          <  2:1466464/92‑1 (MQ=255)

                                                                                                       gCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCCAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAATAAGTGTAATTGacg                         <  1:1132924/140‑3 (MQ=255)

                                                                                                       gCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGacg                         <  1:676803/140‑3 (MQ=255)

                                                                                                       gCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAg                         <  2:656694/140‑1 (MQ=255)

                                                                                                       gCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAg                         <  2:333445/140‑1 (MQ=255)

                                                                                                       gCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAg                         <  2:117748/140‑1 (MQ=255)

                                                                                                       gCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAg                         <  1:1671537/140‑1 (MQ=255)

                                                                                                       gCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAg                         <  1:676709/140‑1 (MQ=255)

                                                                                                       gCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAg                         <  2:1502179/140‑1 (MQ=255)

                                                                                                       gCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAg                         <  1:1792340/140‑1 (MQ=255)

                                                                                                       gCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAg                         <  1:2188942/140‑1 (MQ=255)

                                                                                                        cTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGTCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACACTGTCCGAAAAAGGAAGATGAAAAAAAGAGTAATTTaata                        >  2:814154/1‑138 (MQ=255)

                                                                                                        cTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGACGa                        >  2:1963157/1‑140 (MQ=255)

                                                                                                        cTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAGa                        <  1:1188618/140‑1 (MQ=255)

                                                                                                        cTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAGa                        >  2:307761/1‑140 (MQ=255)

                                                                                                         tAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAGAc                       <  1:946142/140‑1 (MQ=255)

                                                                                                         tAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAGAc                       <  1:837273/140‑1 (MQ=255)

                                                                                                           gTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATg                                                                            >  2:164786/1‑85 (MQ=255)

                                                                                                           gTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATg                                                                            >  2:666056/1‑85 (MQ=255)

                                                                                                                gatgTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAGACAGCTcac                <  2:39946/140‑1 (MQ=255)

                                                                                                                gatgTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAGACAGCTcac                <  2:441397/140‑1 (MQ=255)

                                                                                                                 atgTTTTTTGCTCTCAGATGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAATAAAATGACAATCATGCAAAAATGTTCAGTGTCCTAATAAGGCAGATGCAAAAAAGTGTAATTGAAGACCGCTcaca               <  1:2239278/140‑1 (MQ=255)

                                                                                                                 atgTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAg                                               <  1:1012133/108‑1 (MQ=255)

                                                                                                                 atgTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAg                                               >  2:1012133/1‑108 (MQ=255)

                                                                                                                 atgTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTa                                >  2:2223028/1‑123 (MQ=255)

                                                                                                                 atgTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAGACAGCTcaca               <  2:245512/140‑1 (MQ=255)

                                                                                                                     tttttGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAGACAGCTCACACAgc           <  1:1218020/140‑1 (MQ=255)

                                                                                                                     tttttGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAGACAGCTCACACAgc           <  1:183396/140‑1 (MQ=255)

                                                                                                                           ctctCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAGACAGCTCACACATCGCATaa     >  2:417059/1‑140 (MQ=255)

                                                                                                                           ctctCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAGACAGCTCACACAGCGCCTaa     >  2:2009114/1‑140 (MQ=255)

                                                                                                                           ctctCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAGACAGCTCACACAGCGCATaa     >  2:610100/1‑140 (MQ=255)

GGCCGCTGACGACCGGCTGTATGCTGATCGGGTGCGGATTTGTCCTTTACTTGCTGTATACGCTGCCGGGATTGATCCCATTTTACGGCCCGTGTTTATTTGGGCTAGTGATGATGTTTTTTGCTCTCAGAGGATTTCAAAAGACGGAGGCTCAGCACCACCAAGCAGGTTAACAAAAAAATGACAATCATGCAAAAAAGTACAGTGTCCGAAAAAGGCAGATGCAAAAAAGTGTAATTGAAGACAGCTCACACAGCGCATAAAAT  >  NZ_CP034943/751263‑751528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`