Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 765,082 T→C 5.2% M265T (ATG→ACG)  lplD → alpha‑galacturonidase LplD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943765,0820TC 5.2% 623.4 / 15.6 191M265T (ATG→ACG) lplDalpha‑galacturonidase LplD
Reads supporting (aligned to +/- strand):  ref base T (91/89);  new base C (5/5);  total (96/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

Only 100 of 191 total aligned reads displayed.

CGCCATATTGACCTGCTGCCTATATTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAGACAGAGACGAGAAACGAAA  >  NZ_CP034943/764946‑765220
                                                                                                                                        |                                                                                                                                          
cGCCATATTGACCTGCTGCCTATATTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGta                                                                                                                                         <  2:755378/140‑1 (MQ=255)
cGCCATATTGACCTGCTGCCTATAGTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGta                                                                                                                                         <  1:1281681/140‑1 (MQ=255)
   cATATTGACCTGCTGCCTATATTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAg                                                                                                                                      >  2:881358/1‑140 (MQ=255)
          aCCTGCTGCCTATATTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATc                                                                                                                               >  2:1626146/1‑140 (MQ=255)
          aCCTGCTGCCTATATTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATc                                                                                                                               >  2:1251850/1‑140 (MQ=255)
          aCCTGCTGCCTATATTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATc                                                                                                                               >  2:1160774/1‑140 (MQ=255)
             tgctgcCTATATTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATcccc                                                                                                                            >  2:712728/1‑140 (MQ=255)
               ctgcCTATATTCCATGAATTCAGCTCGCATTCTGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGGGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGc                                                                                                                          >  1:1052602/1‑140 (MQ=255)
               ctgcCTATATTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGc                                                                                                                          >  1:104243/1‑140 (MQ=255)
               ctgcCTATATTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGc                                                                                                                          >  2:1773091/1‑140 (MQ=255)
               ctgcCTATATTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGc                                                                                                                          >  2:1731856/1‑140 (MQ=255)
               ctgcCTATATTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGc                                                                                                                          >  1:2430737/1‑140 (MQ=255)
               ctgcCTATATTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGc                                                                                                                          >  1:1137025/1‑140 (MQ=255)
               ctgcCTATATTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGGTTGATTTATTTGAAATGTATAGCGCCATCCCCGc                                                                                                                          >  1:2255248/1‑140 (MQ=255)
                 gcatataTTACATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTTGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTg                                                                                                                        <  1:1546504/137‑1 (MQ=255)
                 gcCTATATTCCATGAATTCAGCTCGCATTATGGAGACTCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTg                                                                                                                        <  1:1779588/140‑1 (MQ=255)
                 gcCTATATTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTg                                                                                                                        <  1:1594931/140‑1 (MQ=255)
                        ttCCATGAATTCAGCTCGCATGATGGAGAATCAGGCTATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGa                                                                                                                 >  2:307089/1‑140 (MQ=255)
                         tCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGAc                                                                                                                <  2:1747158/140‑1 (MQ=255)
                         tCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGAc                                                                                                                <  2:453514/140‑1 (MQ=255)
                            ctGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAgg                                                                                                             >  2:1311065/2‑140 (MQ=255)
                            aTGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAgg                                                                                                             >  2:1808702/1‑140 (MQ=255)
                            aTGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAgg                                                                                                             >  2:534811/1‑140 (MQ=255)
                             tGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGATGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCTTGTTGCGTGTGAGTTATTTGAAATGTATAGAGCCATCACCGCTGCGGGTGACAGGc                                                                                                            >  2:1258291/1‑140 (MQ=255)
                             tGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGc                                                                                                            >  2:2124685/1‑140 (MQ=255)
                             tGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGc                                                                                                            >  2:485555/1‑140 (MQ=255)
                             tGAATTCAGCGCGCACTATGGGGAATCAGGATATGAGCTGGAGGGGGAATGCTGGAGGGACAGCGTCTTTTGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAACGTATGGCGCCATCCCTGCTGCGGGTGACAGGc                                                                                                            >  2:2326920/1‑140 (MQ=255)
                             tGAATTCAGCGCGCACTATGGGGAATCAGGATATGAGCTGGAGGGGGAATGCTGGAGGGACAGCGTCTTTTGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAACGTATGGCGCCATCCCTGCTGCGGGTGACAGGc                                                                                                            >  2:1218053/1‑140 (MQ=255)
                             ggAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGc                                                                                                            >  2:428607/2‑140 (MQ=255)
                               aaTTCAGCTCGCATTATGGATAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCAt                                                                                                          <  1:1497578/140‑1 (MQ=255)
                                    aGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGc                                                                                                     >  1:1195315/1‑140 (MQ=255)
                                    aGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGc                                                                                                     <  2:1119128/140‑1 (MQ=255)
                                    aGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGc                                                                                                     >  2:2073711/1‑140 (MQ=255)
                                     gCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCa                                                                                                    <  1:31418/140‑1 (MQ=255)
                                     gCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCa                                                                                                    <  2:1003598/140‑1 (MQ=255)
                                                ggAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTcc                                                                                         <  1:712728/140‑1 (MQ=255)
                                                cgagaCTCAGGAGCTGAGCTCGAGGCGGAACGCTGGAGGGACACTGTCTTTCGGGCGGCACACCGTGGTGCGTTTGCTTTATTTGAAAGGTATAGCGCCATCCCCGCTGCTGGTGACAGGCATCTGGCAGAGTTTCGTcc                                                                                         <  2:1895283/139‑1 (MQ=255)
                                                   gaATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCgg                                                                                      >  2:2422964/1‑140 (MQ=255)
                                                   gaATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCgc                                                                                      >  2:1119238/1‑139 (MQ=255)
                                                    aaTCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTg                                                                                                                        <  2:1952154/105‑1 (MQ=255)
                                                    aaTCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTg                                                                                                                        >  1:1952154/1‑105 (MQ=255)
                                                    aaTCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGc                                                                                                     <  1:363822/124‑1 (MQ=255)
                                                    aaTCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTttcttcaaggc                                                                                     >  1:836696/1‑135 (MQ=255)
                                                     aTCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGcc                                                                                    >  1:1602404/1‑140 (MQ=255)
                                                      tCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCTCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGccc                                                                                   <  2:296714/140‑1 (MQ=255)
                                                      tCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGccc                                                                                   <  2:474310/140‑1 (MQ=255)
                                                         ggATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTa                                                                                >  1:1384849/1‑140 (MQ=255)
                                                         ggATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTa                                                                                >  2:363554/1‑140 (MQ=255)
                                                               gAGCTAGAGGGGCAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCaa                                                                          <  2:1963712/140‑1 (MQ=255)
                                                                    agagGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGc                                                                     >  1:1090543/1‑140 (MQ=255)
                                                                    agagGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGc                                                                     >  1:1035051/1‑140 (MQ=255)
                                                                    agagGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATGTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGc                                                                     >  1:1076582/1‑140 (MQ=255)
                                                                      agGGGGAAAGCTGGAGGGACTGTGTCTTTCGTTCGGCACACCGTGTTTCGTTTGATTTATTTGAAATGTATAGCGCCATACCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCt                                                                   <  1:818662/140‑1 (MQ=255)
                                                                      agGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCt                                                                   <  2:2419323/140‑1 (MQ=255)
                                                                      agGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCt                                                                   >  2:1120821/1‑140 (MQ=255)
                                                                      agGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCt                                                                   <  2:55542/140‑1 (MQ=255)
                                                                      agGGGGAAAGCTGGAGGGACAGAGTCTTTCGTTCGGCACACCGTGTTGCGGTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGCTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCt                                                                   >  2:504248/1‑140 (MQ=255)
                                                                             aaGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCaaa                                                                         >  2:1081530/1‑127 (MQ=255)
                                                                                   gAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAAt                                                      >  2:1559314/1‑140 (MQ=255)
                                                                                     gggACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCa                                                                                                    >  1:186391/1‑92 (MQ=255)
                                                                                        aCAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCAc                                                 >  2:1694460/1‑140 (MQ=255)
                                                                                          aGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCt                                               >  1:133397/1‑140 (MQ=255)
                                                                                          aGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCt                                               <  1:686835/140‑1 (MQ=255)
                                                                                          aGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCt                                               <  2:1271546/140‑1 (MQ=255)
                                                                                          aGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCt                                               <  1:168237/140‑1 (MQ=255)
                                                                                          aGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCt                                               <  2:2197267/140‑1 (MQ=255)
                                                                                          aGTGTCTTTCGTTCGGCACACCGTGGTGCGTTTGAGTTATTTGCAATGTATAGCGCCATCCCCGCTGGGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCt                                               <  2:1834999/140‑1 (MQ=255)
                                                                                              tCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTcaca                                           >  1:239296/1‑140 (MQ=255)
                                                                                              tCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTcaca                                           >  1:633719/1‑140 (MQ=255)
                                                                                              tCTTGCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTcaca                                           >  1:1169451/1‑140 (MQ=255)
                                                                                               cTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCacac                                          <  2:104243/140‑1 (MQ=255)
                                                                                               cTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCacac                                          <  2:398061/140‑1 (MQ=255)
                                                                                               cTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTACTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCacac                                          <  2:1980309/140‑1 (MQ=255)
                                                                                                 ttcgttcgGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAACTTTCACCTCACACCt                                        >  2:347513/1‑140 (MQ=255)
                                                                                                 ttcgttcgGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCt                                               >  2:917500/1‑133 (MQ=255)
                                                                                                 ttcgttcgGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCt                                               <  1:917500/133‑1 (MQ=255)
                                                                                                 ttcgttcgGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCt                                        >  2:424501/1‑140 (MQ=255)
                                                                                                           accccGTGTTCCCTTTGATTTAATTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCACAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCa                              <  1:2157238/137‑1 (MQ=255)
                                                                                                           acacCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCa                              <  1:1762002/140‑1 (MQ=255)
                                                                                                           acacCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCa                              <  1:881358/140‑1 (MQ=255)
                                                                                                           acacCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCa                              <  1:1251850/140‑1 (MQ=255)
                                                                                                           acacCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCGGTATCATTCa                              <  2:237286/140‑1 (MQ=255)
                                                                                                                gtgtTGCGTTTGATTTATTTTCAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACGTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGaaaa                         <  2:1383340/140‑1 (MQ=255)
                                                                                                                gtgtTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGaaaa                         <  1:1646604/140‑1 (MQ=255)
                                                                                                                gtgtTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGaaaa                         <  1:214331/140‑1 (MQ=255)
                                                                                                                gtgtTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGaaaa                         <  1:295447/140‑1 (MQ=255)
                                                                                                                     gCGTTTGATTTATTTGAAACGTATGGCGCCATCCCTGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCATACCTCAAACAGCCTGAAGTATGGAAATTTCATCTCACACCTATATCATTCAGAAAACAAGa                    <  1:2326920/140‑1 (MQ=255)
                                                                                                                       ggttGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAGACa                  >  2:187935/3‑140 (MQ=255)
                                                                                                                         ttGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAGACaga                <  2:1137025/140‑1 (MQ=255)
                                                                                                                         ttGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAGACaga                >  2:151929/1‑140 (MQ=255)
                                                                                                                         ttGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAGACaga                <  2:2430737/140‑1 (MQ=255)
                                                                                                                           gATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAGACAgaga              >  1:97311/1‑140 (MQ=255)
                                                                                                                           gATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAGACAgaga              >  1:737427/1‑140 (MQ=255)
                                                                                                                           gATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAGACAgaga              >  2:286997/1‑140 (MQ=255)
                                                                                                                           gATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAGACAgaga              >  2:727236/1‑140 (MQ=255)
                                                                                                                           gATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAGACAgaga              >  2:841115/1‑140 (MQ=255)
                                                                                                                           gATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAGACAgaga              >  2:2307640/1‑140 (MQ=255)
                                                                                                                                atttGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATGTGGCAGAGTTTCTTCCCGGCCCTTACCTAAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAGACAGAGACgaga         <  2:1497652/140‑1 (MQ=255)
                                                                                                                                atttGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAGACAGAGACgaga         >  1:516001/1‑140 (MQ=255)
                                                                                                                                       aTGTATATCCCCATCAGAGCAGCGGGTGAACGGCATCTGGGAGTGTTTCTTCCCGGCACTTACCTCACAACCCCTTAGGTTTGGAATTTTCAACTCACACCTGTATCATTCAGAAAACAAGACCGAGACGAGAAACGaat  <  2:2226277/140‑2 (MQ=255)
                                                                                                                                        |                                                                                                                                          
CGCCATATTGACCTGCTGCCTATATTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAGACAGAGACGAGAAACGAAA  >  NZ_CP034943/764946‑765220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: