Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 765,087 A→G 5.3% S267G (AGC→GGC)  lplD → alpha‑galacturonidase LplD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943765,0870AG 5.3% 612.8 / 15.8 188S267G (AGC→GGC) lplDalpha‑galacturonidase LplD
Reads supporting (aligned to +/- strand):  ref base A (91/87);  new base G (5/5);  total (96/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01

Only 100 of 194 total aligned reads displayed.

CATATTGACCTGCTGCCTATATTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAGACAGAGACGAGAAACGAAAAGAAA  >  NZ_CP034943/764949‑765225
                                                                                                                                          |                                                                                                                                          
cATATTGACCTGCTGCCTATATTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAg                                                                                                                                           >  2:881358/1‑140 (MQ=255)
cATATTGACCTGCTGCCTATATTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAg                                                                                                                                           >  2:1185855/1‑140 (MQ=255)
    ttGACCTGCTGCCTATATTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGcc                                                                                                                                       <  1:2399677/140‑1 (MQ=255)
       aCCTGCTGCCTATATTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATc                                                                                                                                    >  2:1626146/1‑140 (MQ=255)
          tgctgcCTATATTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATcccc                                                                                                                                 >  2:712728/1‑140 (MQ=255)
            ctgcCTATATTCCATGGATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGc                                                                                                                               >  1:2263760/1‑140 (MQ=255)
            ctgcCTATATTCCATGAATTCAGCTCGCATTCTGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGGGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGc                                                                                                                               >  1:1052602/1‑140 (MQ=255)
            ctgcCTATATTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGc                                                                                                                               >  1:104243/1‑140 (MQ=255)
            ctgcCTATATTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGc                                                                                                                               >  1:1137025/1‑140 (MQ=255)
            ctgcCTATATTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGc                                                                                                                               >  2:1773091/1‑140 (MQ=255)
            ctgcCTATATTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGc                                                                                                                               >  2:1731856/1‑140 (MQ=255)
              gcCTATATTCCATGAATTCAGCTCGCATTATGGAGACTCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTg                                                                                                                             <  1:1779588/140‑1 (MQ=255)
              gcCTATATTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTg                                                                                                                             <  1:221265/140‑1 (MQ=255)
               ccTATATTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGc                                                                                                                            <  1:2377031/140‑1 (MQ=255)
                      tCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGAc                                                                                                                     <  2:1747158/140‑1 (MQ=255)
                      tCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGAc                                                                                                                     <  2:1137277/140‑1 (MQ=255)
                         ctGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAgg                                                                                                                  >  2:1311065/2‑140 (MQ=255)
                         aTGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAgg                                                                                                                  >  2:442883/1‑140 (MQ=255)
                         aTGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAgg                                                                                                                  >  2:2359073/1‑140 (MQ=255)
                         aTGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAgg                                                                                                                  >  2:534811/1‑140 (MQ=255)
                          tGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGc                                                                                                                 >  2:485555/1‑140 (MQ=255)
                                 aGCTCGCATTATGGAGTATCCGGATCTGAGCTAGAGGGTGTAAGCTGGAGGGTCAGTGTGTTTCGTTCGGCACACCGTGGTGCGTTTGTTTTATTTGACATGTATAGCGCCTTCCCCGCTGCGGGTGACAGGCATCTGGc                                                                                                          <  2:1222314/140‑1 (MQ=255)
                                 aGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGc                                                                                                          <  2:1119128/140‑1 (MQ=255)
                                 aGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGc                                                                                                          >  1:1195315/1‑140 (MQ=255)
                                  gCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCa                                                                                                         <  2:1003598/140‑1 (MQ=255)
                                  gCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCa                                                                                                         <  1:31418/140‑1 (MQ=255)
                                             ggAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATACCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTcc                                                                                              <  2:349452/140‑1 (MQ=255)
                                                 aaTCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTg                                                                                                                             >  1:1952154/1‑105 (MQ=255)
                                                 aaTCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTg                                                                                                                             <  2:1952154/105‑1 (MQ=255)
                                                 aaTCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGc                                                                                                          <  1:363822/124‑1 (MQ=255)
                                                 aaTCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGc                                                                                                          >  2:363822/1‑124 (MQ=255)
                                                 aaTCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTttcttcaaggc                                                                                          >  1:836696/1‑135 (MQ=255)
                                                 aaTCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTCCCCGGc                                                                                          >  1:166259/1‑140 (MQ=255)
                                                   tCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGccc                                                                                        <  2:296674/140‑1 (MQ=255)
                                                   tCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGccc                                                                                        <  2:1130844/140‑1 (MQ=255)
                                                   tCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGccc                                                                                        <  2:474310/140‑1 (MQ=255)
                                                   tCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGccc                                                                                        <  2:12554/140‑1 (MQ=255)
                                                      ggATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTa                                                                                     >  2:283599/1‑140 (MQ=255)
                                                       gATATGAGCTAGAGGGGGAAAGCTGGAGGGTCAGTGTCTTTCGTTCGGCACACGGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTAc                                                                                    <  2:2426394/140‑1 (MQ=255)
                                                            gAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCaa                                                                               <  2:2089617/140‑1 (MQ=255)
                                                                 agagGGGGAACGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGc                                                                          >  2:2302025/1‑140 (MQ=255)
                                                                 agagGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGccc                                                                                        <  2:1267925/126‑1 (MQ=255)
                                                                 agagGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGc                                                                          >  1:1090543/1‑140 (MQ=255)
                                                                 agagGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGc                                                                          >  1:1035051/1‑140 (MQ=255)
                                                                 agagGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATGTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGc                                                                          >  1:1076582/1‑140 (MQ=255)
                                                                   agGGGGAAAGCTGGAGGGACTGTGTCTTTCGTTCGGCACACCGTGTTTCGTTTGATTTATTTGAAATGTATAGCGCCATACCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCt                                                                        <  1:818662/140‑1 (MQ=255)
                                                                   agGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCt                                                                        <  1:1563553/140‑1 (MQ=255)
                                                                   agGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCt                                                                        <  2:1120719/140‑1 (MQ=255)
                                                                   agGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCt                                                                        <  2:55542/140‑1 (MQ=255)
                                                                   agGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCt                                                                        <  2:2419323/140‑1 (MQ=255)
                                                                   agGGGGAAAGCTGGAGGGACAGAGTCTTTCGTTCGGCACACCGTGTTGCGGTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGCTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCt                                                                        >  2:504248/1‑140 (MQ=255)
                                                                         aaaGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGtt                                                                  >  1:1529525/1‑140 (MQ=255)
                                                                          aaGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCaaa                                                                              >  2:1081530/1‑127 (MQ=255)
                                                                                gAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAAt                                                           >  2:1559314/1‑140 (MQ=255)
                                                                                  gggACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCa                                                                                                         <  2:186391/92‑1 (MQ=255)
                                                                                  gggACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCa                                                                                                         >  1:186391/1‑92 (MQ=255)
                                                                                     aCAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCAc                                                      >  2:1694460/1‑140 (MQ=255)
                                                                                       aGTGTCTTTCGTTCGGCTCACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCt                                                    <  2:1815312/140‑1 (MQ=255)
                                                                                       aGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCt                                                    <  1:168237/140‑1 (MQ=255)
                                                                                       aGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCt                                                    <  1:686835/140‑1 (MQ=255)
                                                                                       aGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCt                                                    <  2:1271546/140‑1 (MQ=255)
                                                                                       aGTGTCTTTCGGTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCt                                                    <  2:303729/140‑1 (MQ=255)
                                                                                           tCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTcaca                                                >  1:633719/1‑140 (MQ=255)
                                                                                           tCTTGCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTcaca                                                >  1:1169451/1‑140 (MQ=255)
                                                                                            cTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCacac                                               <  2:104243/140‑1 (MQ=255)
                                                                                              ttcgttcgGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATATCGACATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGTAAATTTCACCTCTCACCt                                             >  2:1954427/1‑140 (MQ=255)
                                                                                              ttcgttcgGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAACTTTCACCTCACACCt                                             >  2:347513/1‑140 (MQ=255)
                                                                                              ttcgttcgGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCt                                                    >  2:917500/1‑133 (MQ=255)
                                                                                              ttcgttcgGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCt                                                    <  1:917500/133‑1 (MQ=255)
                                                                                              ttcgttcgGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCt                                             >  2:2009713/1‑140 (MQ=255)
                                                                                              ttcgttcgGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCt                                             >  2:2212459/1‑140 (MQ=255)
                                                                                              atcgttcgGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTCCCTCAAACAGCCTGAGGTGTGGAAATTTCACCTCACACCt                                             >  2:424511/2‑140 (MQ=255)
                                                                                                        acacCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCTCTGAGGGTGACAGGCATCTGGCAGAGTTTGTTCCGGGCCCTTACCTCAAACAGCCTGAGTTTTGGAAATTTCACCTCACACCTGTATAATTCa                                   <  2:2140456/140‑1 (MQ=255)
                                                                                                        acacCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTTCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCa                                   <  2:763710/140‑1 (MQ=255)
                                                                                                        acacCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCa                                   <  1:1251850/140‑1 (MQ=255)
                                                                                                        acacCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCa                                   <  1:1762002/140‑1 (MQ=255)
                                                                                                        acacCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCa                                   <  1:881358/140‑1 (MQ=255)
                                                                                                        acacCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCGGTATCATTCa                                   <  2:237286/140‑1 (MQ=255)
                                                                                                             gtgtTGCGTTTGATTTATTTTCAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACGTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGaaaa                              <  2:1383340/140‑1 (MQ=255)
                                                                                                             gtgtTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGaaaa                              <  1:295447/140‑1 (MQ=255)
                                                                                                             gtgtTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGaaaa                              <  2:1892376/140‑1 (MQ=255)
                                                                                                             gtgtTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGaaaa                              <  2:1968021/140‑1 (MQ=255)
                                                                                                             gtgtTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGaaaa                              <  1:214331/140‑1 (MQ=255)
                                                                                                                 tGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAg                          >  1:1597144/1‑140 (MQ=255)
                                                                                                                  gCGTTTGATTTATTTGAAACGTATGGCGCCATCCCTGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCATACCTCAAACAGCCTGAAGTATGGAAATTTCATCTCACACCTATATCATTCAGAAAACAAGa                         <  1:715562/140‑1 (MQ=255)
                                                                                                                  gCGTTTGATTTATTTGAAACGTATGGCGCCATCCCTGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCATACCTCAAACAGCCTGAAGTATGGAAATTTCATCTCACACCTATATCATTCAGAAAACAAGa                         <  1:1299165/140‑1 (MQ=255)
                                                                                                                      ttGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAGACaga                     >  2:151929/1‑140 (MQ=255)
                                                                                                                      ttGATTAATTTGAAATCTATAGCGCCATCCACGATGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGAATCATTCAGAAAACAAGACaga                     >  1:1634477/1‑140 (MQ=255)
                                                                                                                        gATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAGACAgaga                   >  2:841115/1‑140 (MQ=255)
                                                                                                                        gATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAGACAgaga                   >  2:727236/1‑140 (MQ=255)
                                                                                                                        gATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAGACAgaga                   >  1:737427/1‑140 (MQ=255)
                                                                                                                        gATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAGACAgaga                   >  1:97311/1‑140 (MQ=255)
                                                                                                                        gATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAGACAgaga                   >  2:418904/1‑140 (MQ=255)
                                                                                                                        gATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTAGCATTCAGAAAACAAGACAgaga                   >  2:2320841/1‑140 (MQ=255)
                                                                                                                             atttGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATGTGGCAGAGTTTCTTCCCGGCCCTTACCTAAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAGACAGAGACgaga              <  2:1497652/140‑1 (MQ=255)
                                                                                                                             atttGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAGACAGAGACgaga              >  1:516001/1‑140 (MQ=255)
                                                                                                                                    aTGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAGACAGAGACGAGAAACGaaa       <  1:1346002/140‑1 (MQ=255)
                                                                                                                                         taGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAGACAGAGACGAGAAACGAAAAGaaa  <  1:1400954/140‑1 (MQ=255)
                                                                                                                                         taGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAGACAGAGACGAGAAACGAAAAGaaa  <  2:317690/140‑1 (MQ=255)
                                                                                                                                         taGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAGACAGAGACGAGAAACGAAAAGaaa  <  2:1804270/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
CATATTGACCTGCTGCCTATATTCCATGAATTCAGCTCGCATTATGGAGAATCAGGATATGAGCTAGAGGGGGAAAGCTGGAGGGACAGTGTCTTTCGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAATGTATAGCGCCATCCCCGCTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCTTACCTCAAACAGCCTGAGGTTTGGAAATTTCACCTCACACCTGTATCATTCAGAAAACAAGACAGAGACGAGAAACGAAAAGAAA  >  NZ_CP034943/764949‑765225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: