Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 767,723 A→C 5.4% I81S (ATT→AGT)  EO946_RS03930 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943767,7230AC 5.4% 361.0 / 3.0 146I81S (ATT→AGT) EO946_RS03930hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (76/58);  new base C (2/10);  total (78/68)
Fisher's exact test for biased strand distribution p-value = 1.28e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 150 total aligned reads displayed.

CGCACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAA  >  NZ_CP034943/767593‑767861
                                                                                                                                  |                                                                                                                                          
cgcACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgc                                                                                                                                   >  2:2342281/1‑140 (MQ=255)
cgcACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgc                                                                                                                                   >  1:738629/1‑140 (MQ=255)
  cACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCTGGGCCGGACCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccc                                                                                                                                 >  2:1045935/1‑139 (MQ=255)
  cACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCCCCAATACCAGGGCCGGCGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccg                                                                                                                                 >  1:159967/1‑140 (MQ=255)
        cgtcgcgaGGCACAGCGCCGCCGGCACTACCAGGCCCGGCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGccgccgccg                                                                                                                           <  2:850085/133‑1 (MQ=17)
        cgAAGCCAGGCACAGCGCCGCCGCCAATCCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGccgccgccg                                                                                                                           <  1:79957/140‑1 (MQ=255)
        cgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGccgccgccg                                                                                                                           <  1:2413013/140‑1 (MQ=255)
        cgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGccgccgccg                                                                                                                           <  2:1798344/140‑1 (MQ=255)
        cgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGccgccgccg                                                                                                                           <  2:1369582/140‑1 (MQ=255)
          aaGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGCAGGCAGGCACAGCGCCGGAACCACAGCCAGAgccggcggaaaa                                                                                                                         >  2:1722418/1‑135 (MQ=14)
          aaGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCACTGCCAGGGCCGCCGCCGaa                                                                                                                         <  1:67362/140‑1 (MQ=255)
          aaGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGaa                                                                                                                         <  1:2327879/140‑1 (MQ=255)
           aGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGCAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAg                                                                                                                        <  2:2251935/140‑1 (MQ=255)
           aGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAg                                                                                                                        <  2:774436/140‑1 (MQ=255)
           aGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAg                                                                                                                        <  2:1041247/140‑1 (MQ=255)
             ccAGGCACAGCGCCTCCGGCAATTCCAGGGCCGGTGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGcc                                                                                                                      <  2:1133099/140‑1 (MQ=255)
             ccAGGCACAGCGCCGCCGCCAATCCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGcc                                                                                                                      <  2:1713870/140‑1 (MQ=255)
             ccAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGcc                                                                                                                      <  1:1169625/140‑1 (MQ=255)
             ccAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGcc                                                                                                                      <  1:348415/140‑1 (MQ=255)
             ccAGGCACAGCGCCGCCGCCAAGACCCGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGcc                                                                                                                      <  2:937683/140‑1 (MQ=255)
                 gCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGc                                                                                                                  >  1:1656257/1‑140 (MQ=255)
                 gCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGCCCGGAGCCGCAGCCCACCACAGGAACAGCGACTACACAAAAGCCAGAGCCGACCCCGAATCCCGGc                                                                                                                  >  1:1231550/1‑140 (MQ=9)
                   acaGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATCCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGcac                                                                                                                <  1:2098687/140‑1 (MQ=255)
                      gcgcCGCCGCCAATACCAGCGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATCCCAGCGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTCCCAATGCCCGGGCCGCCGCCGACGCCCGGcgccgg                                                                                                             >  1:1270124/1‑135 (MQ=17)
                       cgccgccgcCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGCGCCGCCGCCGAAGCCAGGCCCAGCGCGGCTCCCACTGCGTGGGGCGCGGCCGGCGCCGGGGACAgcg                                                                                                            >  2:1294818/1‑140 (MQ=14)
                               ccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTAcc                                                                                                    <  1:2342281/140‑1 (MQ=255)
                               ccAATACCAGGGCCCGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTAcc                                                                                                    <  2:1175065/140‑1 (MQ=255)
                                cAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCa                                                                                                   <  2:1656257/140‑1 (MQ=255)
                                         ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAggg                                                                                          >  1:1604290/1‑140 (MQ=255)
                                           ccGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTCCCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgcc                                                                                        >  1:1700777/1‑140 (MQ=255)
                                           ccGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGCGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATCCCAGGgcc                                                                                        >  1:1701012/1‑140 (MQ=255)
                                           ccGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATCCCAGGGCCGCCGCCGAACCCAGGCACAGCGCCGCAACCAAAGCCATGgcc                                                                                        >  1:1471803/1‑140 (MQ=18)
                                              gAGCCGCCGCCGAAGCCAGGCACCGCGCCGCCGCCAATCCCAGGGCCGGAGCCGCCGCCGAACCCAGGCACAGCGCCGCTCCCAATCCCAGGGCCGCCGCCGACGCCAGGCACAGCGCCGCTCCCACAGCCACGgccgcc                                                                                     >  2:2010138/1‑140 (MQ=17)
                                              gAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccgcc                                                                                     >  2:897807/1‑140 (MQ=255)
                                              gAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGCCACAGCGCCGCTCCCCATGCCAGGgccgcc                                                                                     >  2:1793159/1‑140 (MQ=255)
                                              gAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCACGGCCGCCGCCCAAGCCAGGCCCAGCCCCGCGACCAATGCCAGagcagcg                                                                                     >  2:1652363/1‑139 (MQ=18)
                                               aGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccgccg                                                                                    <  1:505791/140‑1 (MQ=255)
                                                    ccgccgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGgccgccgccgcc                                                                               >  1:1982485/1‑140 (MQ=255)
                                                    ccgccgAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCAACCAATGCCAGGgccgccgccgcc                                                                               >  1:1982476/1‑140 (MQ=255)
                                                             ccAGGCACAGCGCCGCCGCCACTACCAGGGCCGGAGCCGCCGCCGTACCCAGGCACAGCGCCGCGAGCAATGCGAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGcc                                                                      <  2:705874/140‑1 (MQ=18)
                                                             ccAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGcc                                                                      <  1:897807/140‑1 (MQ=255)
                                                             ccAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGcc                                                                      <  1:1793159/140‑1 (MQ=255)
                                                                       cgccgccgcCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTCCCAATGCCAGGGCCGCCGCCGAAGCCGGGCACAGCGCCGCTCGCAAGGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCCCAgcg                                                            >  2:2146460/1‑140 (MQ=21)
                                                                       cgccgccgcCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCCGGCACAgcg                                                            >  2:2072479/1‑140 (MQ=255)
                                                                       cgccgccgcCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAgcg                                                            >  1:1891941/1‑140 (MQ=255)
                                                                       cgccgccgcCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAgcg                                                            >  1:565342/1‑140 (MQ=255)
                                                                       cgccgccgcCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCACGCACAccg                                                            >  1:215508/1‑140 (MQ=255)
                                                                       cgccgccgcCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAAGGCCCGAGCCGGCGCCCAAGCCAGGCACAGCCCCGGAACCAAGGCCAGGGCCCCCGCCGCCCCCGCAGACAGGcccaccc                                                            >  2:406059/1‑137 (MQ=17)
                                                                               ccAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCGACCAATGCCAGGGCCGCCGCCGACGCCAGGCACAGCGCCGCGACCAAACACAGCGCCGCCGCCGACGCCGAAGCCAGGCACACAgccgccgcc                                                    >  1:558829/1‑140 (MQ=21)
                                                                                 aaTACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCaa                                                  >  1:984500/1‑140 (MQ=255)
                                                                                   tACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATg                                                >  2:809350/1‑140 (MQ=255)
                                                                                   tACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCAACCAAGTCCACGGCCGCCGCCGCCACAGAATCAAGGCAAAGCGCCTCCGCCAAtc                                                >  2:1780791/1‑139 (MQ=18)
                                                                                     cccgggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGcc                                              <  1:215607/137‑1 (MQ=255)
                                                                                     ccAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGcc                                              <  2:1891941/140‑1 (MQ=255)
                                                                                         ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAggg                                          >  2:1933820/1‑140 (MQ=255)
                                                                                         ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAggg                                          >  2:901497/1‑140 (MQ=255)
                                                                                         ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAggg                                          >  2:2151099/1‑140 (MQ=255)
                                                                                         ggCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGG‑‑‑GCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGcag                                       >  2:2168251/1‑138 (MQ=255)
                                                                                          gCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGc                                         >  2:1483678/1‑140 (MQ=255)
                                                                                          gCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGc                                         >  2:159710/1‑140 (MQ=255)
                                                                                             ggAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACCGCCCCGCTCCCAATGCCAGGGCCGCCGCCGCCGCCGACGCCAGGCACAGCCCCGCCGCCAAAGCCAGGGCCgg                                      >  2:955582/1‑140 (MQ=18)
                                                                                              gAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGccgccgccg                                                                                                                           <  2:2258244/54‑1 (MQ=33)
                                                                                              gAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGccgccgccg                                                                                                                           >  1:2258244/1‑54 (MQ=33)
                                                                                              gAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTCCCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGa                                     >  1:1043125/1‑140 (MQ=255)
                                                                                              gAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCTCCAATGCCAGGGCCGGa                                     >  2:281985/1‑140 (MQ=255)
                                                                                              gAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGa                                     >  2:1473774/1‑140 (MQ=255)
                                                                                                        cgACGCCAGGCACCGCGCCGCGACCACTGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATCCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGccgccgccg                           <  2:365377/140‑1 (MQ=18)
                                                                                                        cgAAGCGAGGCACAGCCCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCACGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGccgccgccg                           <  1:1456844/140‑1 (MQ=255)
                                                                                                        cgAAGCCAGGCTCAGCGCCGCGACCAAGGCGAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGccgccgccg                           <  1:1547896/140‑1 (MQ=255)
                                                                                                        cgAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGccgccgccg                           <  2:1604290/140‑1 (MQ=255)
                                                                                                        cgAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCACGCACAGCGCCGCTACCACTCCCAGGGCCCCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGccgccgccg                           <  1:1045935/140‑1 (MQ=255)
                                                                                                        cgAAGCCAGGCACAGCGCCGCGACCACGGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGccgccgccg                           <  1:1022197/140‑1 (MQ=255)
                                                                                                        cgAAGCCAGGCACAGCGCCGCGACCAAGGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGccgccgccg                           <  2:1988734/140‑1 (MQ=255)
                                                                                                        cgAAGCCAGCCACAGCCCCGCTACCAATGCCAGGGCCGCCGCCGCAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGccgccgccg                           <  1:1119573/140‑1 (MQ=255)
                                                                                                                ggCACAGCGCCGCTACCAAGGCCAGGGCCGCCGCCGCAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAgg                   <  2:329675/140‑1 (MQ=255)
                                                                                                                cgCACAGCGCCCCTACCAATGCCAGCGCCGCCTCCGAAGCCAGGCACAGCGCCGCTACCAATGCCACGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAgg                   <  1:1765610/139‑1 (MQ=255)
                                                                                                                 gCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGcc                                              >  1:270808/1‑112 (MQ=255)
                                                                                                                 gCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGcc                                              <  2:270808/112‑1 (MQ=255)
                                                                                                                 gCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGcc                                              >  1:271095/1‑112 (MQ=255)
                                                                                                                     aGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAg              >  1:839078/1‑140 (MQ=255)
                                                                                                                       cgccgcTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGGCGCCGCCCAAGCCACGCACAgcg            >  2:1948124/1‑140 (MQ=255)
                                                                                                                       cgccgcTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAgcg            >  1:958834/1‑140 (MQ=255)
                                                                                                                       cgccgcTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAgcg            >  1:1025623/1‑140 (MQ=255)
                                                                                                                       cgccgcTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAgcg            >  1:731470/1‑140 (MQ=255)
                                                                                                                       cgccgcTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAgcg            >  1:2321084/1‑140 (MQ=255)
                                                                                                                       cgccgcTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGCGCCGGAGCCGCCGCCGAAGCCAGGCACAgcg            >  1:1574622/1‑140 (MQ=255)
                                                                                                                            cTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGcgccg                                                         >  1:2037256/1‑90 (MQ=255)
                                                                                                                            cTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGcgccg                                                         <  2:2037256/90‑1 (MQ=255)
                                                                                                                              tccAAGGCCAGGGCCGCCGCCGAAGCCAGCCACAGCGCCGCCACCCATGCCAGGGCCGGCGCCGCCGCCGAAGGCAGGCACAGCGACGCCGCCACTTCCACGGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccgccgc     <  1:1878512/139‑1 (MQ=14)
                                                                                                                              aCCACTGCCAGGGCCGCCGCCGAAGCCAGCCACAGCGCCGCCACCACTGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGTGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccgccgc     <  1:357949/140‑1 (MQ=17)
                                                                                                                              aCCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccgccgc     <  1:2358295/140‑1 (MQ=255)
                                                                                                                              aCCAAGGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCCGGGCCGGCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCACTGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGcgccgccgc     <  1:38167/140‑1 (MQ=255)
                                                                                                                               ccACTGCCAGGGTCCCCGCCGAAGCCAGGCACATCGGCGCCACCAATCCGAGGGCCGGCGCCGCCGGCGCAGCCAGGCACAGAGCCTGCGCCACTGGCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCgccgccgcc    <  1:2097858/140‑1 (MQ=14)
                                                                                                                               ccACTGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTCCCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCgccgccgcc    <  1:378751/140‑1 (MQ=255)
                                                                                                                               ccAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCgccgccgcc    <  1:2210536/140‑1 (MQ=255)
                                                                                                                               ccAAGGCCCGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCCATCCCAGGGCCGGCGCCGCCGCCGACGCCAGGCCCAGCGGCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCgccgccgcc    <  2:1976061/140‑1 (MQ=17)
                                                                                                                               ccAAGGCCAGGGGCGCCGCCGAAGCCTGGCACATCGCCTGCACCCCTGGCACGGCCCGGGCGGGCGCGGCAGCCTGGCACAGGGCCGGCGCCCAGGCCAGGGCCGGTGCCGCCGCCGAAGCCAGGCACAGCgccgccgcc    <  1:2427678/140‑1 (MQ=9)
                                                                                                                                 aaTGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCaa  >  1:2019960/1‑140 (MQ=255)
                                                                                                                                 aaTGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGCGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGAACAGCGCCGCCGCCaa  >  1:1675324/1‑140 (MQ=255)
                                                                                                                                 aaTGCCAGGGCCGCCGCAGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCAGCAGCCGCCGCCGAAGCCAGGCACATAGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCAGCCGCCaa  >  1:946304/1‑140 (MQ=17)
                                                                                                                                  |                                                                                                                                          
CGCACCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATACCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGAAGCCAGGCACAGCGCCGCTACCAATGCCAGGGCCGCCGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAATGCCAGGGCCGGAGCCGCCGCCGAAGCCAGGCACAGCGCCGCCGCCAA  >  NZ_CP034943/767593‑767861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: