Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 828,787 G→A 100% E77K (GAA→AAA)  treC → alpha,alpha‑phosphotrehalase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943828,7870GA98.7% 542.6 / ‑2.7 154E77K (GAA→AAA) treCalpha,alpha‑phosphotrehalase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (81/71);  total (82/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 155 total aligned reads displayed.

GATCTGAACGGCATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTGAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGATTTCGTCTAAAG  >  NZ_CP034943/828652‑828922
                                                                                                                                       |                                                                                                                                       
 aTCTGAACGGCATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATCAGGGGTATGACATTCGTGATGATTACTCGCTTTATCCTAAATAc                                                                                                                                    >  2:1242217/1‑140 (MQ=255)
 aTCTGAACGGCATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATAc                                                                                                                                    >  2:1106409/1‑140 (MQ=255)
 aTCTGAACGGCATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATAc                                                                                                                                    >  2:2166023/1‑140 (MQ=255)
   cTGAACGGCATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACgg                                                                                                                                  >  2:341072/1‑140 (MQ=255)
   cTGAACGGCATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACgg                                                                                                                                  >  2:206075/1‑140 (MQ=255)
     gAACGGCATTATTGAAAATCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGa                                                                                                                                <  1:414108/140‑1 (MQ=255)
           cATTATTGAAAAGCTTGATTACTTAACAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATgg                                                                                                                          <  2:1207267/140‑1 (MQ=255)
           cATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATgg                                                                                                                          >  2:416346/1‑140 (MQ=255)
           cATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATgg                                                                                                                          >  1:2404247/1‑140 (MQ=255)
           cATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATgg                                                                                                                          >  1:1669701/1‑140 (MQ=255)
                ttGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGGTTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGAt                                                                                                                     >  1:456641/1‑140 (MQ=255)
                ttGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGAt                                                                                                                     >  1:809339/1‑140 (MQ=255)
                     aaGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGAt                                                                                                                            >  1:2303179/1‑128 (MQ=255)
                     aaGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGAt                                                                                                                            <  2:2303179/128‑1 (MQ=255)
                     aaGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGa                                                                                                                >  1:715513/1‑140 (MQ=255)
                      aGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAg                                                                                                               <  1:2058810/140‑1 (MQ=255)
                      aGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAg                                                                                                               <  1:1007168/140‑1 (MQ=255)
                        cTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCg                                                                                                             >  1:2172595/1‑140 (MQ=255)
                        cTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCg                                                                                                             >  2:276131/1‑140 (MQ=255)
                            aTTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTa                                                                                                         <  1:605540/140‑1 (MQ=255)
                            aTTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTa                                                                                                         <  1:1933268/140‑1 (MQ=255)
                                   aaaaaCCCGTCAGGTAGATGTGCTTTGGCGGACACCGATGTCTGATTCTCCGCAGCAGGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCg                                                                                                  <  1:736357/140‑1 (MQ=255)
                                   aaaaaCACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCg                                                                                                  >  1:176494/1‑140 (MQ=255)
                                   aaaaaCACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCg                                                                                                  <  2:773130/140‑1 (MQ=255)
                                   aaaaaCACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCg                                                                                                  <  1:2310447/140‑1 (MQ=255)
                                   aaaaaCACTTCAGGTAGATGTGCGTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCg                                                                                                  <  1:2141926/140‑1 (MQ=255)
                                     aaaCGCTACAGGTGGATGTGCTTTGGCTGACGCCGATTTATGATTCTCCCCAGCATGATAATGGGTACGATATTCGTGATTATTACTCGATTTATCCTGAATACGGAACGATGGAGGATTTTGAGCGGCTAGTGTCCGaa                                                                                                <  2:2330906/140‑1 (MQ=255)
                                           ttCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCAt                                                                                          >  1:1994158/1‑140 (MQ=255)
                                           ttCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCAt                                                                                          >  2:771048/1‑140 (MQ=255)
                                           ttCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCAt                                                                                          >  2:726801/1‑140 (MQ=255)
                                            tCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATa                                                                                         <  1:980860/140‑1 (MQ=255)
                                                gTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATCATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAag                                                                                     <  1:1289342/140‑1 (MQ=255)
                                                     tgtgCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGcc                                                                                >  2:1156989/1‑140 (MQ=255)
                                                     tgtgCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTATGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGcc                                                                                >  2:1065662/1‑140 (MQ=255)
                                                           ttGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAg                                                                                                               <  2:1377051/103‑1 (MQ=255)
                                                           ttGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGaa                                                                                                <  1:987213/118‑1 (MQ=255)
                                                            tGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAgt                                                                         <  2:1886805/140‑1 (MQ=255)
                                                             ggCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGAttt                                                                                                                   >  2:1116720/1‑97 (MQ=255)
                                                             ggCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGAttt                                                                                                                   <  1:1116720/97‑1 (MQ=255)
                                                                        aTTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCt                                                             >  1:156547/1‑140 (MQ=255)
                                                                        aTTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCt                                                             >  1:385881/1‑140 (MQ=255)
                                                                        aTTTATGATTCTCCGCAGCATGATAATGGGTATGACATACGTGATGATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCt                                                             >  1:1073149/1‑140 (MQ=255)
                                                                             tGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTgtcg                                                        >  2:1769107/1‑140 (MQ=255)
                                                                             tGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTgtcg                                                        >  2:2262991/1‑140 (MQ=255)
                                                                              gATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTgtcgt                                                       <  1:416346/140‑1 (MQ=255)
                                                                              gATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTgtcgt                                                       <  2:2283475/140‑1 (MQ=255)
                                                                              gATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTgtcgt                                                       <  1:137553/140‑1 (MQ=255)
                                                                                  ctcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAAt                                                   <  1:341072/140‑1 (MQ=255)
                                                                                  ctcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAAt                                                   <  1:1132553/140‑1 (MQ=255)
                                                                                  ctcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAAt                                                   <  1:1528021/140‑1 (MQ=255)
                                                                                  ctcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAAt                                                   <  2:1540157/140‑1 (MQ=255)
                                                                                  ctcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAAt                                                   >  2:1132553/1‑140 (MQ=255)
                                                                                  ctcCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAAt                                                   <  1:2047213/140‑1 (MQ=255)
                                                                                    ccGCAGCATGATCATGGGTAGGACATTCGTGATTATTACTCGATTTATCCTAAATCCGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCa                                                 <  1:1317473/140‑1 (MQ=255)
                                                                                    ccGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCa                                                 <  1:58050/140‑1 (MQ=255)
                                                                                    ccGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCa                                                 <  1:1464355/140‑1 (MQ=255)
                                                                                          caTGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACAt                                            <  2:1519607/139‑1 (MQ=255)
                                                                                            ttaTAATGGGTATGACATTAGTGATTATTACTCGATTTATCCTAAATAGGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCaa                                         <  2:376958/138‑1 (MQ=255)
                                                                                            tGATAATTGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCaa                                         <  2:1793470/140‑1 (MQ=255)
                                                                                            tGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCaa                                         <  2:543358/140‑1 (MQ=255)
                                                                                             gATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAAt                                                   >  2:2172644/1‑129 (MQ=255)
                                                                                             gATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAAt                                                   <  1:2172644/129‑1 (MQ=255)
                                                                                              aTAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACa                                       <  1:209095/140‑1 (MQ=255)
                                                                                               tAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTAAAGACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACag                                      <  2:1604865/140‑1 (MQ=255)
                                                                                                    ggTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAag                                                                                     >  2:1549878/1‑88 (MQ=255)
                                                                                                    ggTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAag                                                                                     <  1:1549878/88‑1 (MQ=255)
                                                                                                    ggTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGTATCTTGTCGTCAATAATAAATCAACAGAGcac                                 >  2:1349454/1‑140 (MQ=255)
                                                                                                    ggTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATc                                                              <  1:1615378/111‑1 (MQ=255)
                                                                                                    ggTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATc                                                              >  2:1615378/1‑111 (MQ=255)
                                                                                                    ggTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGcac                                 >  1:121347/1‑140 (MQ=255)
                                                                                                    ggTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGcac                                 >  2:2020831/1‑140 (MQ=255)
                                                                                                    ggTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGcac                                 >  2:1852425/1‑140 (MQ=255)
                                                                                                    ggTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGcac                                 >  1:2268078/1‑140 (MQ=255)
                                                                                                    ggTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGcac                                 >  1:2140278/1‑140 (MQ=255)
                                                                                                    ggTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGcac                                 >  2:1330736/1‑140 (MQ=255)
                                                                                                    ggTATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATAATAAATCAACAGAGcac                                 >  2:2127406/1‑140 (MQ=255)
                                                                                                      tATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGCCGAGGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCATGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACaa                               >  2:604752/1‑140 (MQ=255)
                                                                                                      tATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACaa                               >  2:655741/1‑140 (MQ=255)
                                                                                                      tATGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACaa                               <  1:1684907/140‑1 (MQ=255)
                                                                                                      gaTGTCATTCGTGATTATTAGTCGATTTATCCTAAATACGTGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATATTGTCGTCAATCATACATCAACAGAGCACaa                               <  2:1431617/139‑1 (MQ=255)
                                                                                                      gaTGACATTCGTGATTATTACTCGATTTATCCGACATACGCGCCGATGGAGGATTTTGAGCGGTTACGGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACaa                               <  1:2101726/139‑1 (MQ=255)
                                                                                                       aTGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACaaa                              >  2:958548/1‑140 (MQ=255)
                                                                                                        tGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAAt                             >  2:765292/1‑140 (MQ=255)
                                                                                                        tGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAAt                             >  1:1196025/1‑140 (MQ=255)
                                                                                                        tGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAAt                             >  1:2318720/1‑140 (MQ=255)
                                                                                                        tGACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATAAATCAACAGAGCACAAAt                             >  1:85325/1‑140 (MQ=255)
                                                                                                         gACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGACGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATg                            >  2:38691/1‑140 (MQ=255)
                                                                                                         gACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATg                            >  2:1977166/1‑140 (MQ=255)
                                                                                                         gACATTCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATg                            >  2:1489453/1‑140 (MQ=255)
                                                                                                              tCGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGATGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGttta                       >  2:2388236/1‑139 (MQ=255)
                                                                                                              tCGGGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTc                       >  2:2433801/1‑140 (MQ=255)
                                                                                                               cGTGATTATTACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAAt                                                   <  1:1162986/111‑1 (MQ=255)
                                                                                                                gTGATTATTACTCGATTTTTCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCaa                     <  2:2406114/140‑1 (MQ=255)
                                                                                                                   attattACTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAagag                  >  2:470615/1‑140 (MQ=255)
                                                                                                                     tattaCTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGc                >  2:2407678/1‑140 (MQ=255)
                                                                                                                     tattaCTCGATTAATCCTAAATACGTGACGATGGAGGATTTTGAGCGGTTACTGTCCTAAGCTCATAAAAGAGGCCTGAAATTCTTCATGGATCTAGTCGTCAAACATACATCAACAGAGCACAAAAGGTTTCAAGAGGc                >  2:551386/1‑140 (MQ=255)
                                                                                                                          cTCGATTTATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGAttt           <  2:2037240/140‑1 (MQ=255)
                                                                                                                               tttATCCTACATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGATTTCGTCt      <  1:1311098/140‑1 (MQ=255)
                                                                                                                               tttATCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGATTTCGTCt      >  1:1466344/1‑140 (MQ=255)
                                                                                                                                   tCCTAAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGATTTCGTCTAAAg  <  2:151581/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
GATCTGAACGGCATTATTGAAAAGCTTGATTACTTAAAAACACTTCAGGTAGATGTGCTTTGGCTGACACCGATTTATGATTCTCCGCAGCATGATAATGGGTATGACATTCGTGATTATTACTCGATTTATCCTGAATACGGGACGATGGAGGATTTTGAGCGGTTACTGTCCGAAGCTCATAAAAGAGGCCTGAAAGTCGTCATGGATCTTGTCGTCAATCATACATCAACAGAGCACAAATGGTTTCAAGAGGCGATTTCGTCTAAAG  >  NZ_CP034943/828652‑828922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: