Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 892,039 T→C 6.0% L230L (CTT→CTC EO946_RS04510 → iron ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943892,0390TC 6.0% 530.4 / 15.8 165L230L (CTT→CTCEO946_RS04510iron ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (104/51);  new base C (5/5);  total (109/56)
Fisher's exact test for biased strand distribution p-value = 3.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 165 total aligned reads displayed.

CCGGCTCTGTGCAGGGCAGGAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCA  >  NZ_CP034943/891903‑892175
                                                                                                                                        |                                                                                                                                        
  ggatctGTGCAGGGCAGGAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCa                                                                                                                                     <  2:545386/137‑1 (MQ=255)
         tgCAGGGCAGGAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCACAAGGACTTGGCCAAAAAACg                                                                                                                              >  2:467472/1‑140 (MQ=255)
         tgCAGGGCAGGAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAGGGACTTGGCCAAAAAACg                                                                                                                              >  1:108072/1‑140 (MQ=255)
         tgCAGGGCAGGAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACg                                                                                                                              >  1:111908/1‑140 (MQ=255)
         tgCAGGGCAGGAGTCTGGATCTGATGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACg                                                                                                                              >  2:618529/1‑140 (MQ=255)
              ggcaggAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCct                                                                                                                         >  1:2295291/1‑140 (MQ=255)
              ggcaggAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCct                                                                                                                         >  1:1199274/1‑140 (MQ=255)
              ggcaggAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCct                                                                                                                         >  2:1003679/1‑140 (MQ=255)
              ggcaggAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCct                                                                                                                         >  1:1734258/1‑140 (MQ=255)
                   gAGTCTGGATCTGTTGATGACGATGTTTCCATATGCAGCGGCAGCGCTGGTAATCTGCTTCTTTCTCGGCCAAAAAATTAATCTCCTCGTTCTGGGAGAAGATGTGGCAAAAGGACTCGGCCAAAAAACAGGCCTGCTGa                                                                                                                    >  2:1737212/1‑140 (MQ=255)
                   gAGTCTGGATCTGTTGATGACGATGTTTCCATATGCAGCGGCAGCGCTGGTAATCTGCTTCTTTCTCGGCCAAAAAATTAATCTCCTCGTTATGGGAGAAGATGTGGCAAAAGGACTCGGCCAAAAAACAGGCCTGCTGa                                                                                                                    >  2:2035512/1‑140 (MQ=255)
                   gAGTCTGGATCTGTTGATGACGATGTTTCCATATGCAGCGGCAGCGCTGGTAATCTGCTTCTTTCTCGGCCAAAAAATTAATCTCCTCGTTATGGGAGAAGATGTGGCAAAAGGACTCGGCCAAAAAACAGGCCTGCTGa                                                                                                                    >  2:1644489/1‑140 (MQ=255)
                   gAGTCTGGATCTGCTGATGACCATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATAAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAACACGGGCCTGCTGa                                                                                                                    >  2:2105253/1‑140 (MQ=255)
                   gAGTCTGGATCTGCTGATGACAATGCTCCCATATGCGGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGa                                                                                                                    >  2:785243/1‑140 (MQ=255)
                   gAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGa                                                                                                                    >  1:1031297/1‑140 (MQ=255)
                   gAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGa                                                                                                                    >  2:1978645/1‑140 (MQ=255)
                   gAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGa                                                                                                                    >  1:960651/1‑140 (MQ=255)
                   gAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGa                                                                                                                    >  1:2132495/1‑140 (MQ=255)
                   gAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGa                                                                                                                    >  1:543932/1‑140 (MQ=255)
                   gAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGa                                                                                                                    >  1:2274673/1‑140 (MQ=255)
                   gAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGa                                                                                                                    >  2:843363/1‑140 (MQ=255)
                   gAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGa                                                                                                                    >  2:721761/1‑140 (MQ=255)
                   gAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGa                                                                                                                    >  1:1458067/1‑140 (MQ=255)
                   gAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGa                                                                                                                    >  1:1331458/1‑140 (MQ=255)
                   gAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGa                                                                                                                    >  2:864822/1‑140 (MQ=255)
                   gAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCAGGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGa                                                                                                                    >  2:2220624/1‑140 (MQ=255)
                       cTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAAtt                                                                                                                <  1:445513/140‑1 (MQ=255)
                       cTGGATCTGCTGATGACAATGCTCCCATAGGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAAtt                                                                                                                <  1:2199748/140‑1 (MQ=255)
                        tGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAAttt                                                                                                               >  2:1228531/1‑140 (MQ=255)
                        tGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAAttt                                                                                                               >  2:674940/1‑140 (MQ=255)
                          gATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTtgt                                                                                                             >  1:2021806/1‑140 (MQ=255)
                              tgctgATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATg                                                                                                         >  2:164482/1‑140 (MQ=255)
                              tgctgATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATg                                                                                                         <  2:349993/140‑1 (MQ=255)
                               gatgatgaCAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCATGCTGAAATTTGTGATgg                                                                                                        >  2:672075/3‑140 (MQ=255)
                                ctgATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGACATTTGTGATGGc                                                                                                       >  2:2427729/1‑140 (MQ=255)
                                ctgATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGc                                                                                                       >  1:1958823/1‑140 (MQ=255)
                                ctgATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGc                                                                                                       >  1:2308896/1‑140 (MQ=255)
                                ctgATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGc                                                                                                       >  2:2338616/1‑140 (MQ=255)
                                ctgATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGc                                                                                                       >  2:1343981/1‑140 (MQ=255)
                                 tgatgaCAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCa                                                                                                      <  2:1336652/140‑1 (MQ=255)
                                  gatgaCAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCAc                                                                                                     >  2:1716858/1‑140 (MQ=255)
                                  gatgaCAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCAc                                                                                                     >  2:2148159/1‑140 (MQ=255)
                                        aaTGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCg                                                                                               <  2:1788607/140‑1 (MQ=255)
                                        aaTGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCg                                                                                               <  2:831189/140‑1 (MQ=255)
                                          tGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCgtt                                                                                             >  1:599983/1‑140 (MQ=255)
                                                cATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTg                                                                                       <  1:2022941/140‑1 (MQ=255)
                                                cATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTg                                                                                       <  1:979821/140‑1 (MQ=255)
                                                 atatGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGc                                                                                      >  2:1717850/1‑140 (MQ=255)
                                                   atGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTa                                                                                    <  1:672060/140‑1 (MQ=255)
                                                   atGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTa                                                                                    <  1:2421023/140‑1 (MQ=255)
                                                     gCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGc                                                                                  <  1:2048238/140‑1 (MQ=255)
                                                     gCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGc                                                                                  <  1:674940/140‑1 (MQ=255)
                                                      cAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGcc                                                                                 >  1:271005/1‑140 (MQ=255)
                                                      cAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGcc                                                                                 >  1:2150481/1‑140 (MQ=255)
                                                       aGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCg                                                                                >  1:310808/1‑140 (MQ=255)
                                                       aGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCg                                                                                >  1:301337/1‑140 (MQ=255)
                                                       aGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGATAGCCg                                                                                >  2:1630483/1‑140 (MQ=255)
                                                         ccGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAATATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCggg                                                                              >  2:345865/1‑140 (MQ=255)
                                                         ccGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAGATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCggg                                                                              >  1:1749356/1‑140 (MQ=255)
                                                         ccGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTTATGGCACTTTGCGTTGTTTTGCTAGCCggt                                                                              >  2:1425742/1‑139 (MQ=255)
                                                         ccGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCggg                                                                              >  1:2089726/1‑140 (MQ=255)
                                                         ccGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCggg                                                                              >  1:1811512/1‑140 (MQ=255)
                                                         ccGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGGTGTTTTGCTAGCCggg                                                                              >  2:239759/1‑140 (MQ=255)
                                                         ccGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACGTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCAGTTTGCGTTGTTTTGCTAGACggg                                                                              >  1:1843853/1‑140 (MQ=255)
                                                         ccGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATAAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCggg                                                                              >  1:150169/1‑140 (MQ=255)
                                                             aGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTctg                                                                          <  1:1066537/140‑1 (MQ=255)
                                                                 cTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGt                                                                      >  1:719760/1‑140 (MQ=255)
                                                                 cTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGt                                                                      >  1:1498609/1‑140 (MQ=255)
                                                                   tATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCg                                                                    >  1:1033209/1‑140 (MQ=255)
                                                                   tATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCg                                                                    >  1:2178137/1‑140 (MQ=255)
                                                                    aTGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGc                                                                   >  1:655307/1‑140 (MQ=255)
                                                                         cAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTg                                                              >  2:1638273/1‑140 (MQ=255)
                                                                            cttcttTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGCCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATAGCCg                                                           >  2:73043/1‑140 (MQ=255)
                                                                                 ttcttGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCa                                                                                                                                     <  2:1805554/61‑1 (MQ=255)
                                                                                      ggCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTc                                                 >  1:2193013/1‑140 (MQ=255)
                                                                                      ggCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTc                                                 >  1:237055/1‑140 (MQ=255)
                                                                                           aaaaaTCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTa                                            <  2:237055/140‑1 (MQ=255)
                                                                                           aaaaaTCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTa                                            <  1:130440/140‑1 (MQ=255)
                                                                                           aaaaaTCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTa                                            <  2:309803/140‑1 (MQ=255)
                                                                                           aaaaaTCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTa                                            <  2:271005/140‑1 (MQ=255)
                                                                                           aaaaaTCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTa                                            <  2:2193013/140‑1 (MQ=255)
                                                                                           aaaaaTCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTa                                            <  2:2150481/140‑1 (MQ=255)
                                                                                           aaaaaTCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTa                                            <  2:1460045/140‑1 (MQ=255)
                                                                                               atcaatcTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTgg                                        <  1:1228531/140‑1 (MQ=255)
                                                                                                       cctcGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCatta                                >  2:1184168/1‑140 (MQ=255)
                                                                                                       cctcGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCatta                                >  2:2114993/1‑140 (MQ=255)
                                                                                                           gTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTcc                            >  1:324676/1‑140 (MQ=255)
                                                                                                                 ggAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCAttt                      <  2:1941209/140‑1 (MQ=255)
                                                                                                                 ggAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCAttt                      <  2:2244146/140‑1 (MQ=255)
                                                                                                                 ggAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCAttt                      <  2:2095721/140‑1 (MQ=255)
                                                                                                                    gaagaTGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGc                   <  1:785243/140‑1 (MQ=255)
                                                                                                                     aagaTGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGcc                  >  2:1098250/1‑140 (MQ=255)
                                                                                                                            ggCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTgg                                        >  2:1286523/1‑111 (MQ=255)
                                                                                                                            ggCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTgg                                        <  1:1286523/111‑1 (MQ=255)
                                                                                                                               aaaaGGACTCGGCCAAAAAACAGGCCTGCTGAAATTTGTGATGGCGCTTTGTGTTTTCATGCTTGCAGGGTCCGCTGTCGCCATTGCCGGGCCGTTTTCTTTTATCGGCATCATTATTCCGCATTTTGCCCGCTTTGtcg        <  1:2090707/140‑1 (MQ=255)
                                                                                                                               aaaaGGACTCGGCCAAAAAACAGGCCTGCTGAAATTTGTGATGGCGCTTTGTGTTGTCATGCTTGCAGGGTCCGCTGTCGCCATTGCCGGGCCGATTTCTTTTATCGGCATCATTATTCCGCATTTTGCCCGCTTTGtcg        <  1:1737212/140‑1 (MQ=255)
                                                                                                                               aaaaGGACTCGGCCAAAAAACAGGCCTGCTGAAATTTGTGATGGCGCTTTGTGTTGTCATGCTTGCAGGGTCCGCTGTCGCCATTGCCGGGCCGATTTCTTTTATCGGCATCATTATTCCGCATTTTGCCCGCTTTGtcg        <  1:1641286/140‑1 (MQ=255)
                                                                                                                                    gACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGc   <  2:49997/140‑1 (MQ=255)
                                                                                                                                    gACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGc   <  2:2090126/140‑1 (MQ=255)
                                                                                                                                    gACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATGGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGc   <  2:886411/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
CCGGCTCTGTGCAGGGCAGGAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCA  >  NZ_CP034943/891903‑892175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: