Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 892,051 G→A 5.8% T234T (ACG→ACA EO946_RS04510 → iron ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943892,0510GA 5.8% 554.7 / 15.4 173T234T (ACG→ACAEO946_RS04510iron ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (102/60);  new base A (5/5);  total (108/65)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

Only 100 of 179 total aligned reads displayed.

TGCAGGGCAGGAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGG  >  NZ_CP034943/891912‑892189
                                                                                                                                           |                                                                                                                                          
tgCAGGGCAGGAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACg                                                                                                                                            >  1:627001/1‑140 (MQ=255)
     ggcaggAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCct                                                                                                                                       >  1:1745766/1‑140 (MQ=255)
     ggcaggAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCct                                                                                                                                       >  1:1734258/1‑140 (MQ=255)
     ggcaggAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCct                                                                                                                                       >  1:2388540/1‑140 (MQ=255)
     ggcaggAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCct                                                                                                                                       >  1:1199274/1‑140 (MQ=255)
     ggcaggAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCct                                                                                                                                       >  2:125785/1‑140 (MQ=255)
     ggcaggAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCct                                                                                                                                       >  1:704488/1‑140 (MQ=255)
        aggAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCctgct                                                                                                                                    >  2:1345960/1‑140 (MQ=255)
          gAGTCTGGATCTGTTGATGACGATGTTTCCATATGCAGCGGCAGCGCTGGTAATCTGCTTCTTTCTCGGCCAAAAAATTAATCTCCTCGTTCTGGGAGAAGATGTGGCAAAAGGACTCGGCCAAAAAACAGGCCTGCTGa                                                                                                                                  >  2:1737212/1‑140 (MQ=255)
          gAGTCTGGATCTGTTGATGACGATGTTTCCATATGCAGCGGCAGCGCTGGTAATCTGCTTCTTTCTCGGCCAAAAAATTAATCTCCTCGTTATGGGAGAAGATGTGGCAAAAGGACTCGGCCAAAAAACAGGCCTGCTGa                                                                                                                                  >  2:1641286/1‑140 (MQ=255)
          gAGTCTGGATCTGTTGATGACGATGTTTCCATATGCAGCGGCAGCGCTGGTAATCTGCTTCTTTCTCGGCCAAAAAATTAATCTCCTCGTTATGGGAGAAGATGTGGCAAAAGGACTCGGCCAAAAAACAGGCCTGCTGa                                                                                                                                  >  2:1644489/1‑140 (MQ=255)
          gAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTCTGATCAGCTTCTTTCTTGGCCAAAATATCAATCTCCTCGTCATGGGAGAAGATGTGGCCAAAGGACTTGGCCAAAAAACGGGCCTGCTGa                                                                                                                                  >  2:873512/1‑140 (MQ=255)
          gAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGa                                                                                                                                  >  2:1978645/1‑140 (MQ=255)
          gAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGa                                                                                                                                  >  1:543932/1‑140 (MQ=255)
          gAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGa                                                                                                                                  >  1:1031297/1‑140 (MQ=255)
          gAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGa                                                                                                                                  >  1:2274673/1‑140 (MQ=255)
          gAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGa                                                                                                                                  >  1:2259250/1‑140 (MQ=255)
          gAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGa                                                                                                                                  >  2:721761/1‑140 (MQ=255)
          gAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGa                                                                                                                                  >  1:116849/1‑140 (MQ=255)
          gAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGa                                                                                                                                  >  2:843363/1‑140 (MQ=255)
          gAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGa                                                                                                                                  >  1:1331458/1‑140 (MQ=255)
          gAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGa                                                                                                                                  >  1:1458067/1‑140 (MQ=255)
             tCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAAt                                                                                                                               >  1:2090126/1‑140 (MQ=255)
             tCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAAt                                                                                                                               >  1:49997/1‑140 (MQ=255)
               tGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAAttt                                                                                                                             >  2:674940/1‑140 (MQ=255)
               tGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAAttt                                                                                                                             >  2:1228531/1‑140 (MQ=255)
                 gATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTtgt                                                                                                                           >  1:2021806/1‑140 (MQ=255)
                     tgctgATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATg                                                                                                                       >  2:483582/1‑140 (MQ=255)
                      gctgATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATgg                                                                                                                      >  2:672060/1‑140 (MQ=255)
                      gctgATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATgg                                                                                                                      >  2:824042/1‑140 (MQ=255)
                       ctgATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGACATTTGTGATGGc                                                                                                                     >  2:2427729/1‑140 (MQ=255)
                       ctgATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGc                                                                                                                     >  1:218125/1‑140 (MQ=255)
                       ctgATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGc                                                                                                                     >  1:1958823/1‑140 (MQ=255)
                       ctgATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGc                                                                                                                     >  2:2338616/1‑140 (MQ=255)
                       ctgATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGc                                                                                                                     >  1:2308896/1‑140 (MQ=255)
                        tgatgaCAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCa                                                                                                                    <  2:1336652/140‑1 (MQ=255)
                               aaTGCTCCCCTATGCAGCCTCAGCGCTTATGATGAGCTTCTTTCTTGGCCAACAAATCTATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTTCTGAAATTTGTGATGGCACTTTGCg                                                                                                             <  2:2197893/140‑1 (MQ=255)
                               aaTGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCg                                                                                                             <  2:1788607/140‑1 (MQ=255)
                               aaTGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCg                                                                                                             <  2:831189/140‑1 (MQ=255)
                               aaTGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCg                                                                                                             <  2:218125/140‑1 (MQ=255)
                                 tGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCgtt                                                                                                           >  1:216349/1‑140 (MQ=255)
                                    tCCCATATGCATCCGCAGCGCTTATGATCAGCTTCTTTATTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACTGGCCTGCTGAAATTTGTGATGGCCCTTTGCgttgtg                                                                                                        >  1:2115533/1‑139 (MQ=255)
                                       cATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTg                                                                                                     <  1:1267964/140‑1 (MQ=255)
                                       cATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTg                                                                                                     <  1:979821/140‑1 (MQ=255)
                                        atatGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGc                                                                                                    >  2:1717850/1‑140 (MQ=255)
                                          atGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTa                                                                                                  <  1:672060/140‑1 (MQ=255)
                                            gCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGc                                                                                                <  1:674940/140‑1 (MQ=255)
                                            gCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGc                                                                                                <  1:2048238/140‑1 (MQ=255)
                                             cAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGcc                                                                                               >  1:1460045/1‑140 (MQ=255)
                                             cAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGcc                                                                                               >  1:271005/1‑140 (MQ=255)
                                             cAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGcc                                                                                               >  1:2150481/1‑140 (MQ=255)
                                              aGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCg                                                                                              >  1:310808/1‑140 (MQ=255)
                                              aGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCg                                                                                              >  1:301337/1‑140 (MQ=255)
                                                ccGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAATATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCggg                                                                                            >  2:345865/1‑140 (MQ=255)
                                                ccGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAGATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCggg                                                                                            >  1:1749356/1‑140 (MQ=255)
                                                ccGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTTATGGCACTTTGCGTTGTTTTGCTAGCCggt                                                                                            >  2:1425742/1‑139 (MQ=255)
                                                ccGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGGTGTTTTGCTAGCCggg                                                                                            >  2:239759/1‑140 (MQ=255)
                                                ccGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACGTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCAGTTTGCGTTGTTTTGCTAGACggg                                                                                            >  1:1843853/1‑140 (MQ=255)
                                                ccGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATAAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCggg                                                                                            >  1:150169/1‑140 (MQ=255)
                                                   cAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTct                                                                                         >  2:1066537/1‑140 (MQ=255)
                                                    aGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTctg                                                                                        <  1:1066537/140‑1 (MQ=255)
                                                          tATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCg                                                                                  >  1:1033209/1‑140 (MQ=255)
                                                           aTGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGc                                                                                 >  1:911363/1‑140 (MQ=255)
                                                                cAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTg                                                                            >  2:1451105/1‑140 (MQ=255)
                                                                cAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTg                                                                            >  2:454143/1‑140 (MQ=255)
                                                                   cttcttTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCg                                                                         >  2:130440/1‑140 (MQ=255)
                                                                   cttcttTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGCCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATAGCCg                                                                         >  2:73043/1‑140 (MQ=255)
                                                                             ggCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTc                                                               >  1:237055/1‑140 (MQ=255)
                                                                                  aaaaaTCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTa                                                          <  1:978703/140‑1 (MQ=255)
                                                                                  aaaaaTCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTa                                                          <  2:2150481/140‑1 (MQ=255)
                                                                                    aaaTCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTAtt                                                        <  2:213261/140‑1 (MQ=255)
                                                                                              cctcGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGGCGGGCCGATTTCATTTATTGGCATCatta                                              >  2:2114983/1‑140 (MQ=255)
                                                                                               ctcGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCattat                                             >  1:1287227/1‑140 (MQ=255)
                                                                                                        ggAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGc                                                                      <  1:227940/106‑1 (MQ=255)
                                                                                                        ggAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCAttt                                    <  2:2095721/140‑1 (MQ=255)
                                                                                                        ggAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCAttt                                    <  2:2350393/140‑1 (MQ=255)
                                                                                                        ggAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCAttt                                    <  2:1956343/140‑1 (MQ=255)
                                                                                                        ggAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCAttt                                    <  2:1941209/140‑1 (MQ=255)
                                                                                                        ggAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCAttt                                    <  2:2244146/140‑1 (MQ=255)
                                                                                                           gaagaTGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGc                                 <  2:1711834/140‑1 (MQ=255)
                                                                                                           gaagaTGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGc                                 <  2:111908/140‑1 (MQ=255)
                                                                                                            aagaTGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGcc                                <  2:1631804/140‑1 (MQ=255)
                                                                                                                     cAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTAtc                       <  2:301337/140‑1 (MQ=255)
                                                                                                                     cAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTAtc                       <  2:310808/140‑1 (MQ=255)
                                                                                                                      aaaaGGACTCGGCCAAAAAACAGGCCTGCTGAAATTTGTGATGGCGCTTTGTGTTGTCATGCTTGCAGGGTCCGCTGTCGCCATTGCCGGGCCGATTTCTTTTATCGGCATCATTATTCCGCATTTTGCCCGCTTTGtcg                      <  1:1641286/140‑1 (MQ=255)
                                                                                                                      aaaaGGACTCGGCCAAAAAACAGGCCTGCTGAAATTTGTGATGGCGCTTTGTGTTGTCATGCTTGCAGGGTCCGCTGTCGCCATTGCCGGGCCGATTTCTTTTATCGGCATCATTATTCCGCATTTTGCCCGCTTTGtcg                      <  1:2035512/140‑1 (MQ=255)
                                                                                                                        aaGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTAtcgtc                    >  2:615698/1‑140 (MQ=255)
                                                                                                                         aGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCTTTATTCCGCATTTTGCCCGATTTAtcgtcg                   >  1:349448/1‑140 (MQ=255)
                                                                                                                           gACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGc                 <  2:2090126/140‑1 (MQ=255)
                                                                                                                            aCTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCa                <  1:1098250/140‑1 (MQ=255)
                                                                                                                                ggCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGa            <  2:911363/140‑1 (MQ=255)
                                                                                                                                ggCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGa            <  2:655307/140‑1 (MQ=255)
                                                                                                                                      aacaCGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTAGTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCg      <  1:284182/137‑1 (MQ=255)
                                                                                                                                      aaaaCGGGCCTGCTGACATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCg      >  1:743826/1‑140 (MQ=255)
                                                                                                                                      aaaaCGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCg      >  1:2193740/1‑140 (MQ=255)
                                                                                                                                      aaaaCGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCg      >  1:993154/1‑140 (MQ=255)
                                                                                                                                        aaCGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGtt    <  2:1796835/140‑1 (MQ=255)
                                                                                                                                        aaCGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGtt    <  2:150169/140‑1 (MQ=255)
                                                                                                                                        aaCGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGtt    <  2:20654/140‑1 (MQ=255)
                                                                                                                                          cGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGAGTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTgg  >  1:794974/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TGCAGGGCAGGAGTCTGGATCTGCTGATGACAATGCTCCCATATGCAGCCGCAGCGCTTATGATCAGCTTCTTTCTTGGCCAAAAAATCAATCTCCTCGTCATGGGAGAAGATGTGGCAAAAGGACTTGGCCAAAAAACGGGCCTGCTGAAATTTGTGATGGCACTTTGCGTTGTTTTGCTAGCCGGGTCTGCTGTCGCAATTGCCGGGCCGATTTCATTTATTGGCATCATTATTCCGCATTTTGCCCGATTTATCGTCGGCAATGATTATCGTTGG  >  NZ_CP034943/891912‑892189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: