Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 296,608 C→G 6.0% A201A (GCC→GCG EO946_RS01660 → DUF4901 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943296,6080CG 6.0% 330.2 / 5.7 101A201A (GCC→GCGEO946_RS01660DUF4901 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (52/42);  new base G (4/3);  total (56/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

GGGTCGCTGATGAAGAAGAGGCATTTGCCTTCATCAAGAAGGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGAAAAGGCTGATCTTGGCAGTATGATCGGCATCGAA  >  NZ_CP034943/296469‑296746
                                                                                                                                           |                                                                                                                                             
gggTCGCTTATGAAGAAGACTCATTTGCCTTCATCAATAAGGACGTGGAGTTCGACCTTTTGTTTGAGGTCCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGcc                                                                                                                                               <  2:2029875/140‑1 (MQ=255)
gggTCGCTGATGAAGAAGAGGCATTTGCCTTCATCAAGAAGGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGcc                                                                                                                                               <  2:1620355/140‑1 (MQ=255)
  gTCGCTGATGAAGAAGAGGCATTTGCCTTCCTCAAGAAGGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCACCCGCCTTTGGTGTATGAGCCTGAATGCAGGGCCGt                                                                                                                                             >  1:1909676/1‑140 (MQ=255)
  gTCGCTGATGAAGAAGAGGCATTTGCCTTCATCAAGAAGGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAATCGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGt                                                                                                                                             >  1:1936723/1‑140 (MQ=255)
  gTCGCTGATGAAGAAGAGGCATTTGCCTTCATCAAGAAGGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTCTGAGCCTGAATGCAGGGCCGt                                                                                                                                             >  1:2328393/1‑140 (MQ=255)
  gTCGCTGATGAAGAAGAGGCATTTGCCTTCATCAAGAAGGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGt                                                                                                                                             >  1:1660720/1‑140 (MQ=255)
  gTCGCTGATGAAGAAGAGGCATTTGCCTTCATCAAGAAGGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGt                                                                                                                                             >  1:1288251/1‑140 (MQ=255)
  gTCGCTGATGAAGAAGAGGCATTTGCCTTCATCAAGAAGGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGt                                                                                                                                             >  1:2276105/1‑140 (MQ=255)
  gTCGCTGATGAAGAAGAGGCATTTGCCTTCATCAAGAAGGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCAGTTGGTGTATGAGCCTGAATGCAGGGCCGt                                                                                                                                             >  1:1829431/1‑140 (MQ=255)
      cTGATGAAGAAGAGGCATTTGCCTTCATCAAGAAGGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTAcc                                                                                                                                         <  1:1911283/140‑1 (MQ=255)
             agaagaGGCATTTGCCTTCATCAAGAAGGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGtgcctg                                                                                                                                  <  2:951631/140‑1 (MQ=255)
                 gagGCATTTGCCTTCATCAAGATGGACGTGGAGTTCGACCTTTTGGTTGTGGTTCTCCATCGTTCTGTTTACAATAACGGGGACGTTCAACCGCATTTGGGGGATGAGCCTGAATGCAGGGCGGTTACCGTGCCTGCCgc                                                                                                                              >  2:1077832/1‑139 (MQ=255)
                 gagGCATTTGCCTTCATCAAGAAGGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGTCGTTACCGTGCCTGCCGa                                                                                                                              >  2:2281183/1‑140 (MQ=255)
                 gagGCATTTGCCTTCATCAAGAAGGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGa                                                                                                                              >  2:689314/1‑140 (MQ=255)
                 gagGCATTTGCCTTCATCAAGAAGGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGa                                                                                                                              >  2:1971170/1‑140 (MQ=255)
                 gagGCATTTGCCTTCATCAAGAAGGACGTGGAGTTCGACCGTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGa                                                                                                                              >  2:2415971/1‑140 (MQ=255)
                  cggCATTTGCCTTCATCAAGAAGGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGGATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGAt                                                                                                                             <  1:880925/139‑1 (MQ=255)
                  agGCATTTGCCTTCATCAAGAAGGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGAt                                                                                                                             <  1:438555/140‑1 (MQ=255)
                  agGCATTTGCCTTCATCAAGAAGGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGAt                                                                                                                             <  1:2145589/140‑1 (MQ=255)
                  agGCATTTGCCTTCATCAAGAAGGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGAt                                                                                                                             <  1:2145583/140‑1 (MQ=255)
                      aTTTGCCTTCATCAAGAAGGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTgg                                                                                                                         >  1:607865/1‑140 (MQ=255)
                       ttttCCTTCATCAAGAAGGACTTGGAGTTCCACATTTTGTTTGAGGTTATCCATCTTTCTGTTTACACAAACGGGGACGATCAACCGAATTTGGTGTATGAGCCTGAATTCAGGGCCGTTACCGTGCCTGCCGATTTGGt                                                                                                                        <  2:1403613/140‑1 (MQ=255)
                                  caAGAAGGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAAc                                                                                                             <  2:732479/140‑1 (MQ=255)
                                   aagaagGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCTCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACa                                                                                                            <  2:1335883/140‑1 (MQ=255)
                                   aagaagGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACa                                                                                                            <  2:2036552/140‑1 (MQ=255)
                                   aagaagGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACa                                                                                                            <  2:347709/140‑1 (MQ=255)
                                   aagaagGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACa                                                                                                            <  1:2414593/140‑1 (MQ=255)
                                   aagaagGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACa                                                                                                            <  2:1036227/140‑1 (MQ=255)
                                   aagaagGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACa                                                                                                            <  1:1527719/140‑1 (MQ=255)
                                     gaagGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGAGATGATCAGCCGCATTTGGTGTACGAGCCTGAAGGTAGGGCGATTACCGTACCTGCGGATTTGGTTCAAGAAGAACAAg                                                                                                          <  2:1814970/140‑1 (MQ=255)
                                      aagGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGc                                                                                                         >  1:1794919/1‑140 (MQ=255)
                                      aagGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGAGTTGGTGAAAGAAGAACAAGc                                                                                                         >  1:1872471/1‑140 (MQ=255)
                                           cGTGTAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAaag                                                                                                                   <  2:406621/125‑1 (MQ=255)
                                           cGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAaag                                                                                                                   >  1:406621/1‑125 (MQ=255)
                                           cGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTtg                                                                                                    >  1:1843671/1‑140 (MQ=255)
                                           cGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTtg                                                                                                    >  1:1843744/1‑140 (MQ=255)
                                           cGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTtg                                                                                                    >  1:1601750/1‑140 (MQ=255)
                                                aGTTCGACCTTTTGTTTGAGGTTCTCCATGGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTtgatgat                                                                                               <  1:425545/140‑1 (MQ=255)
                                                aGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTtgatgat                                                                                               <  1:1308366/140‑1 (MQ=255)
                                                    cGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATgatgacg                                                                                           >  2:2201418/1‑140 (MQ=255)
                                                    cGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATgatgacg                                                                                           >  1:1277631/1‑140 (MQ=255)
                                                    cGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATGTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATgatgacg                                                                                           >  1:2208115/1‑140 (MQ=255)
                                                     gACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATgatgacga                                                                                          >  2:1054246/1‑140 (MQ=255)
                                                             gtttgAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAggg                                                                               <  2:1277631/140‑1 (MQ=255)
                                                                tgAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGc                                                                            >  2:2118117/1‑140 (MQ=255)
                                                                  aGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCg                                                                          <  1:1266749/140‑1 (MQ=255)
                                                                        tcCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCGGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGCTGATGACGATG‑‑‑ACAGGGAGCCGGAAAGc                                                                    >  1:72641/1‑140 (MQ=255)
                                                                        tcCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATGTGGTGAAAGAAGAAAAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGc                                                                    >  1:1660178/1‑140 (MQ=255)
                                                                              gTTCTGTTTACAAACACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCct                                                              <  1:2231524/140‑1 (MQ=255)
                                                                              gTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCct                                                              <  1:1280023/140‑1 (MQ=255)
                                                                              gTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCct                                                              <  1:635109/140‑1 (MQ=255)
                                                                              gTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCct                                                              <  1:646548/140‑1 (MQ=255)
                                                                              gTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCct                                                              <  1:284061/140‑1 (MQ=255)
                                                                              gTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCct                                                              >  1:235539/1‑140 (MQ=255)
                                                                              gTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCct                                                              <  1:1408082/140‑1 (MQ=255)
                                                                              gTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCct                                                              <  2:263134/140‑1 (MQ=255)
                                                                                 cTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCtctc                                                           >  2:1362907/1‑140 (MQ=255)
                                                                                 cTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCtctc                                                           >  2:2116845/1‑140 (MQ=255)
                                                                                    tttACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCt                                                                   <  2:2080664/129‑1 (MQ=255)
                                                                                    tttACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCt                                                                   >  1:2080664/1‑129 (MQ=255)
                                                                                    tttACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCt                                                        >  1:113013/1‑140 (MQ=255)
                                                                                    tttACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCAGTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCt                                                        >  1:1949576/1‑140 (MQ=255)
                                                                                    tttACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCAGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCt                                                        <  2:2059089/140‑1 (MQ=255)
                                                                                              cGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAg                                              >  1:1285143/1‑140 (MQ=255)
                                                                                                       tCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAGGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACg                                     >  2:1548820/1‑140 (MQ=255)
                                                                                                       tCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACg                                     >  2:297555/1‑140 (MQ=255)
                                                                                                       tCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACg                                     >  2:297318/1‑140 (MQ=255)
                                                                                                         aaCCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGaa                                   <  1:1679138/140‑1 (MQ=255)
                                                                                                         aaCCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGaa                                   >  1:1991779/1‑140 (MQ=255)
                                                                                                             gCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGAAAAgg                               <  1:1111557/140‑1 (MQ=255)
                                                                                                             gCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGAAAAgg                               <  1:206687/140‑1 (MQ=255)
                                                                                                             gCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGAAAAgg                               <  1:308293/140‑1 (MQ=255)
                                                                                                             gCATTGGGTGTATGCGCCTGACTGCAGGGCCGGGACCGGCCCTGCCGATTTCCTGAAAGAAGAACACGCATCTGATGATGACGAAG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGAAAAgg                               <  2:2164575/140‑1 (MQ=255)
                                                                                                                  tGGTGTATGAGACTGATTGCTGGGAGGTTACCGTGCCTGCAGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATT‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCTTTCACGAAAATGGTGAt                          <  2:2012292/140‑1 (MQ=255)
                                                                                                                  tGGTGTAGGAGCCTGCATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGACAGACGAACACGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGAAAAGGCTGAt                          <  2:389936/140‑1 (MQ=255)
                                                                                                                       tATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGAAAAGGCTGATCTTgg                     <  2:110858/140‑1 (MQ=255)
                                                                                                                       tATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGAAAAGGCTGATCTTgg                     <  2:111251/140‑1 (MQ=255)
                                                                                                                         tGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGAAAAGGCTGATCTTGGCa                   >  1:1303683/1‑140 (MQ=255)
                                                                                                                         tGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGAAAAGGCTGATCTTGGCa                   >  1:1706913/1‑140 (MQ=255)
                                                                                                                         tGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGAAAAGGCTGATCTTGGCa                   >  1:18195/1‑140 (MQ=255)
                                                                                                                             ccTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTtgatgat                                                                                               >  1:1071291/1‑63 (MQ=255)
                                                                                                                             ccTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGAAAAGGCTGATCTTGGCAGTAt               >  1:416831/1‑140 (MQ=255)
                                                                                                                             ccTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGAAAAGGCTGATCTTGGCAGTAt               >  1:1709147/1‑140 (MQ=255)
                                                                                                                               tGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGAAAAGGCTGATCtt                       >  2:1715247/1‑130 (MQ=255)
                                                                                                                               tGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGAAAAGGCTGATCtt                       >  2:1715171/1‑130 (MQ=255)
                                                                                                                               tGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGAAAAGGCTGATCtt                       <  1:1715247/130‑1 (MQ=255)
                                                                                                                               tGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGAAAAGGCTGATCtt                       <  1:1715171/130‑1 (MQ=255)
                                                                                                                               tGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGAAAAGGCTGATCTTGGcctgtctc             >  2:2019121/1‑133 (MQ=255)
                                                                                                                               tGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGAAAAGGCTGATCTTGGCAGTATGa             >  1:1349339/1‑140 (MQ=255)
                                                                                                                               tGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGGGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGCAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGAAAAGGCTGATAGTGGCAGTATGa             >  1:35807/1‑140 (MQ=255)
                                                                                                                                     cAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGAAAAGGCTGATCTTGGCAGTATGATCGGCa       <  2:666934/140‑1 (MQ=255)
                                                                                                                                     cAGGGCCGTGACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGAACAGGCTGATCTTGGCAGTATGATCGGCa       <  1:675620/140‑1 (MQ=255)
                                                                                                                                      aGGGCGATTACCGTACCTGCGGATTTGGTTCAAGAAGAACAAGCAGTTGATGATGACGATGATTACAGGGAGCCGGAGAGCTTTCCTCTCCCTTTATTCGAGGGCATCCGCGaa                                   >  1:2003669/1‑114 (MQ=255)
                                                                                                                                      aGGGCGATTACCGTACCTGCGGATTTGGTTCAAGAAGAACAAGCAGTTGATGATGACGATGATTACAGGGAGCCGGAGAGCTTTCCTCTCCCTTTATTCGAGGGCATCCGCGaa                                   <  2:2003669/114‑1 (MQ=255)
                                                                                                                                        ggCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGAAAAGGCTGATCTTGGCAGTATGATCGGCATCg    >  1:298230/1‑140 (MQ=255)
                                                                                                                                          ccGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCct                                                              >  2:619213/1‑80 (MQ=255)
                                                                                                                                          ccGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCct                                                              <  1:619213/80‑1 (MQ=255)
                                                                                                                                          ccGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGAAAAGGCTGATCTTGGCAGTATGATCGGGATCgga  >  1:557870/1‑138 (MQ=255)
                                                                                                                                          ccGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGAAAAGGCTGATCTTGGCAGTATGATCGGCATCGaa  >  1:1718987/1‑140 (MQ=255)
                                                                                                                                          ccGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGAAAAGGCTGATCTTGGCAGTATGATCGGCATCGaa  >  1:2216490/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                             
GGGTCGCTGATGAAGAAGAGGCATTTGCCTTCATCAAGAAGGACGTGGAGTTCGACCTTTTGTTTGAGGTTCTCCATCGTTCTGTTTACAAAAACGGGGACGATCAACCGCATTTGGTGTATGAGCCTGAATGCAGGGCCGTTACCGTGCCTGCCGATTTGGTGAAAGAAGAACAAGCATTTGATGATGACGATG‑‑‑ACAGGGAGCCGGAAAGCTTTCCTCTCCCTTTATTCGAAGGCATTCACGAAAAGGCTGATCTTGGCAGTATGATCGGCATCGAA  >  NZ_CP034943/296469‑296746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: