Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 892,896 A→G 5.4% P183P (CCA→CCG EO946_RS04515 → iron ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943892,8960AG 5.4% 544.1 / 13.7 167P183P (CCA→CCGEO946_RS04515iron ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (67/91);  new base G (3/6);  total (70/97)
Fisher's exact test for biased strand distribution p-value = 7.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

Only 100 of 169 total aligned reads displayed.

GCCTCAGCTGTTGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATAC  >  NZ_CP034943/892762‑893035
                                                                                                                                      |                                                                                                                                           
  cTCAGCTGTTGGCCTCATCGTATAGTTACGGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAAGGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACa                                                                                                                                      <  2:1992990/140‑1 (MQ=255)
     aGCTGTTGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACagag                                                                                                                                   <  2:569107/140‑1 (MQ=255)
     aGCTGTTGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACagag                                                                                                                                   <  2:836055/140‑1 (MQ=255)
     aGCTGTTGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACagag                                                                                                                                   >  2:1999849/1‑140 (MQ=255)
     aGCTGTTGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACagag                                                                                                                                   <  2:2416040/140‑1 (MQ=255)
          ttGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTca                                                                                                                              >  2:150711/1‑140 (MQ=255)
          ttGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTca                                                                                                                              <  2:1567944/140‑1 (MQ=255)
          ttGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTca                                                                                                                              <  2:350035/140‑1 (MQ=255)
           tGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTcac                                                                                                                             <  2:571079/140‑1 (MQ=255)
           tGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTcac                                                                                                                             <  2:683091/140‑1 (MQ=255)
               cTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGc                                                                                                                         >  1:539705/1‑140 (MQ=255)
                tCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGcc                                                                                                                        <  1:993069/140‑1 (MQ=255)
                 cATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCa                                                                                                                       <  2:818965/140‑1 (MQ=255)
                 cATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCa                                                                                                                       <  1:18614/140‑1 (MQ=255)
                 cATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCa                                                                                                                       >  2:61086/1‑140 (MQ=255)
                 cATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCa                                                                                                                       <  1:342018/140‑1 (MQ=255)
                 cATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCa                                                                                                                       <  2:1890363/140‑1 (MQ=255)
                 cATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCa                                                                                                                       <  1:315776/140‑1 (MQ=255)
                 cATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCa                                                                                                                       <  1:150711/140‑1 (MQ=255)
                 cATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCa                                                                                                                       <  2:429872/140‑1 (MQ=255)
                 cATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCa                                                                                                                       >  2:2395713/1‑140 (MQ=255)
                  aTCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTcac                                                                                                                             <  1:1862464/133‑1 (MQ=255)
                  aTCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTcac                                                                                                                             >  2:1862464/1‑133 (MQ=255)
                  aTCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCaa                                                                                                                      >  2:1420413/1‑140 (MQ=255)
                   tCGTTTATTTACTGGCATACACAAATGGTGCCTCCACATTTCGGATGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACCGAGCGTCACAAGCCAAt                                                                                                                     <  1:2277790/140‑1 (MQ=255)
                   tCGTTTATTTACTGGCATACAACAATTGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTTAAGGCCCAATTTACAGAGCGTCACAAGCCAAt                                                                                                                     <  1:23091/140‑1 (MQ=255)
                   tCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTTCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAt                                                                                                                     <  1:415186/140‑1 (MQ=255)
                   tCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAt                                                                                                                     <  1:1726883/140‑1 (MQ=255)
                   tCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAt                                                                                                                     <  1:892133/140‑1 (MQ=255)
                   tCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAt                                                                                                                     <  1:1986949/140‑1 (MQ=255)
                   tCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAt                                                                                                                     <  2:1186253/140‑1 (MQ=255)
                   tCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAt                                                                                                                     <  2:736378/140‑1 (MQ=255)
                   tCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAt                                                                                                                     <  1:302251/140‑1 (MQ=255)
                    cGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGTGGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAtg                                                                                                                    >  1:879638/1‑140 (MQ=255)
                     gTTTATTTACTGGCATACATAAATGGTGCCTCCACATGTCGGCTGGTGATTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCGGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAtgt                                                                                                                   <  2:380107/140‑1 (MQ=255)
                        tatttaCTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGta                                                                                                                >  1:1082299/1‑140 (MQ=255)
                            taCTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATAtt                                                                                                            >  2:2107617/1‑140 (MQ=255)
                                   aTACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGt                                                                                                     <  2:483619/140‑1 (MQ=255)
                                   aTACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGt                                                                                                     <  2:458781/140‑1 (MQ=255)
                                    tACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTa                                                                                                                                        <  2:693878/104‑1 (MQ=255)
                                    tACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTa                                                                                                                                        >  1:693878/1‑104 (MQ=255)
                                          aaTGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTa                                                                                              <  2:117513/140‑1 (MQ=255)
                                          aaTGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTa                                                                                              <  2:923698/140‑1 (MQ=255)
                                          aaTGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTa                                                                                              <  2:827064/140‑1 (MQ=255)
                                          aaTGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTa                                                                                              <  2:2291609/140‑1 (MQ=255)
                                             ggcgCCTCCACATTTCGGCTGGTGCTTATCGGAATCGGATTTTCAATGTCGGCGCAGGCGATGACTACCCTGTTGATGATCAAAGGCCCGATTTACCGAGCATCACAAGCCAATGTGTATATCACTGGGTCAGTTTATgg                                                                                           >  2:1846015/4‑140 (MQ=255)
                                              gTGCCTCCACATTTCGGCTGGTGATTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCAGACAACACTGCTGATGATTAAAGGCCCAATTAACAGAGAGTCACAATCCCCAGTGTAAAATACCGGGTCAGTTTATggg                                                                                          >  2:169759/1‑140 (MQ=255)
                                                 ccTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAt                                                                                                                     >  2:2368829/1‑110 (MQ=255)
                                                 ccTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAt                                                                                                                     <  1:2368829/110‑1 (MQ=255)
                                                 ccTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCa                                                                                       >  1:917692/1‑140 (MQ=255)
                                                 ccTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCGGATGATTAAAGGCCCAATTTACAGAGCGTCACACGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCa                                                                                       >  2:2335516/1‑140 (MQ=255)
                                                 ccTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTAACCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCa                                                                                       >  2:1147592/1‑140 (MQ=255)
                                                        aTTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACATAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGgc                                                                                >  2:1327447/1‑140 (MQ=255)
                                                                  gTGCTTATCGGGATCGGATTTTCAATGTCGGGGCAGGCTCTGACTACCCTGCTGATGAGTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATTGGTCAAACTGGCAGCATGTaaa                                                                      >  2:2179431/1‑140 (MQ=255)
                                                                  gTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaa                                                                      >  2:222389/1‑140 (MQ=255)
                                                                  gTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaa                                                                      >  2:1353826/1‑140 (MQ=255)
                                                                    tcTTATCGGAATCGGATTTTCAATGTCGGCGCAGGCGATGACTACCCTGTTGATGATCAAAGGCCCGATTTACCGAGCATCACAAGCCAATGTGTATATCACTGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaaaa                                                                    <  2:2213790/139‑1 (MQ=255)
                                                                    gCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaaaa                                                                    <  1:2101567/140‑1 (MQ=255)
                                                                    gCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaaaa                                                                    <  2:2071014/140‑1 (MQ=255)
                                                                    gCTTATCGGCATCGGATTTTCAATGTCGGCGCAGGCGATGACTACCCTGTTGATGATCAAAGGCCCGATTTACCGAGCATCACAAGCCAATGTGTATATCACTGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaaaa                                                                    <  2:2151325/140‑1 (MQ=255)
                                                                    gCTGATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaaaa                                                                    <  2:1480603/140‑1 (MQ=255)
                                                                     cTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAAt                                                                   <  1:1591690/140‑1 (MQ=255)
                                                                     cTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAAt                                                                   <  1:1357386/140‑1 (MQ=255)
                                                                              aTCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCtattat                                                          <  1:2132701/140‑1 (MQ=255)
                                                                                  taTTTTCAATGTCGGCCCAGGCTCTGACTACCCTGCTGATGATTAAAGTCACAAATTACACAGCGTCACAAGCCAATGTGTATATTAAATGGTCAGTTTATGGGTAAAACTGTCCGCATGTAAAAATAGCTAttaactta                                                      >  2:1227909/2‑140 (MQ=255)
                                                                                  gATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTAttatctta                                                      >  2:1640461/1‑140 (MQ=255)
                                                                                              cGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATtctc                                          <  1:120595/140‑1 (MQ=255)
                                                                                                      gCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTAc                                  <  2:534589/140‑1 (MQ=255)
                                                                                                      gCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTAc                                  <  1:199816/140‑1 (MQ=255)
                                                                                                      gCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGACAATGTGTATATTACCGGGTCATTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTAc                                  <  2:743829/140‑1 (MQ=255)
                                                                                                                cccTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATc                        >  1:1380192/1‑140 (MQ=255)
                                                                                                                cccTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATc                        >  2:1325448/1‑140 (MQ=255)
                                                                                                                cccTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATc                        >  2:1700952/1‑140 (MQ=255)
                                                                                                                 ccTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATcg                       >  1:2196244/1‑140 (MQ=255)
                                                                                                                  ctgctgATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATcgc                      >  2:1568803/1‑140 (MQ=255)
                                                                                                                    gctgATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCgcgc                    >  1:278646/1‑140 (MQ=255)
                                                                                                                          gatTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCTCTGaaaa              >  2:657863/1‑140 (MQ=255)
                                                                                                                          gatTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaa              >  2:242865/1‑140 (MQ=255)
                                                                                                                          gatTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaa              >  2:449807/1‑140 (MQ=255)
                                                                                                                          gatTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaa              >  2:1619347/1‑140 (MQ=255)
                                                                                                                          gatTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCAGTCTCTTATTTACTTGTTTTATCGCGCTGaaaa              >  2:1015191/1‑140 (MQ=255)
                                                                                                                           atTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaaa             <  1:957280/140‑1 (MQ=255)
                                                                                                                           atTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaaa             >  2:415114/1‑140 (MQ=255)
                                                                                                                           atTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaaa             <  1:1420413/140‑1 (MQ=255)
                                                                                                                              caaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAatat          <  1:2042893/139‑1 (MQ=255)
                                                                                                                              aaaGGCCCAATTTACAGATCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAatat          <  1:1187808/140‑1 (MQ=255)
                                                                                                                              aaaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAatat          <  1:1010167/140‑1 (MQ=255)
                                                                                                                              aaaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAatat          <  1:100648/140‑1 (MQ=255)
                                                                                                                               aaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaaa             <  2:2020867/136‑1 (MQ=255)
                                                                                                                               aaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaaa             >  1:2020867/1‑136 (MQ=255)
                                                                                                                               aaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATg         >  1:2030645/1‑140 (MQ=255)
                                                                                                                               aaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATg         >  1:2030625/1‑140 (MQ=255)
                                                                                                                               aaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGGTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATg         >  2:2267900/1‑140 (MQ=255)
                                                                                                                                 ggCCCGATTTACCGAGCATCACAAGCCAATGTGTATATCACTGGGTCAGTTTATGGTTCAAACTGGCAGCTTGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTCATTTGTTTTGTCGCGCTAAAAAATATGaa       <  1:2097375/140‑1 (MQ=255)
                                                                                                                                 ggCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGaa       <  1:1759570/140‑1 (MQ=255)
                                                                                                                                  gCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCTCTCTGAAAAATATTAat      >  2:960402/1‑140 (MQ=255)
                                                                                                                                   cccAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAata     <  1:61086/140‑1 (MQ=255)
                                                                                                                                   cccAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAata     <  1:506268/140‑1 (MQ=255)
                                                                                                                                   cccAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAata     <  1:1286682/140‑1 (MQ=255)
                                                                                                                                      aaTTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATAc  <  1:1581920/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                           
GCCTCAGCTGTTGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATAC  >  NZ_CP034943/892762‑893035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: