Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 892,903 A→C 5.8% R186R (AGA→CGA)  EO946_RS04515 → iron ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943892,9030AC 5.8% 519.8 / 13.3 162R186R (AGA→CGA) EO946_RS04515iron ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (61/91);  new base C (3/7);  total (64/98)
Fisher's exact test for biased strand distribution p-value = 7.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 172 total aligned reads displayed.

CTCAGCTGTTGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTAC  >  NZ_CP034943/892764‑893041
                                                                                                                                           |                                                                                                                                          
   aGCTGTTGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTATAGGCCCAATTTACagag                                                                                                                                         >  2:610297/1‑140 (MQ=255)
   aGCTGTTGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACagag                                                                                                                                         <  2:726366/140‑1 (MQ=255)
   aGCTGTTGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACagag                                                                                                                                         <  2:2416040/140‑1 (MQ=255)
   aGCTGTTGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACagag                                                                                                                                         <  2:836055/140‑1 (MQ=255)
   aGCTGTTGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACagag                                                                                                                                         >  2:1999849/1‑140 (MQ=255)
   aGCTGTTGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACagag                                                                                                                                         <  2:569107/140‑1 (MQ=255)
       gTTGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTc                                                                                                                                     <  2:2319471/140‑1 (MQ=255)
        ttGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTca                                                                                                                                    <  2:350035/140‑1 (MQ=255)
        ttGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTca                                                                                                                                    <  2:1567944/140‑1 (MQ=255)
          ggACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACACTGCTGATGATTAAAGGCCCAATTTACAGAGCGTcaca                                                                                                                                  >  2:1490095/1‑140 (MQ=255)
             cTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGc                                                                                                                               <  2:2184677/140‑1 (MQ=255)
               cATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCa                                                                                                                             >  2:1286682/1‑140 (MQ=255)
               cATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCa                                                                                                                             <  2:1152988/140‑1 (MQ=255)
               cATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCa                                                                                                                             >  1:953217/1‑140 (MQ=255)
               cATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCa                                                                                                                             >  2:61086/1‑140 (MQ=255)
               cATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCa                                                                                                                             <  1:315762/140‑1 (MQ=255)
               cATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCa                                                                                                                             >  1:2327626/1‑140 (MQ=255)
               cATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCa                                                                                                                             <  2:429872/140‑1 (MQ=255)
               cATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCa                                                                                                                             <  2:818965/140‑1 (MQ=255)
               cATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCa                                                                                                                             <  1:18614/140‑1 (MQ=255)
                aTCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTcac                                                                                                                                   <  1:1862464/133‑1 (MQ=255)
                aTCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTcac                                                                                                                                   >  2:1862226/1‑133 (MQ=255)
                aTCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCaa                                                                                                                            >  2:1420413/1‑140 (MQ=255)
                 tCGTTTATTTACTGGCATACACAAATGGTGCCTCCACATTTCGGATGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACCGAGCGTCACAAGCCAAt                                                                                                                           <  1:2277790/140‑1 (MQ=255)
                 tCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTTCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAt                                                                                                                           <  1:415186/140‑1 (MQ=255)
                 tCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAt                                                                                                                           <  1:2109683/140‑1 (MQ=255)
                 tCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAt                                                                                                                           <  1:2136781/140‑1 (MQ=255)
                 tCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAt                                                                                                                           <  2:1186253/140‑1 (MQ=255)
                 tCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATGTCGGCTGGTGGTTATCTGGATCGGATTTTCTATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAt                                                                                                                           <  2:2243338/140‑1 (MQ=255)
                 tCGTTTATTTACTCGCATACAAAAATGGTGCCTCCACATGTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAt                                                                                                                           <  1:229971/140‑1 (MQ=255)
                 tCGTTTATGTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAt                                                                                                                           <  1:1686309/140‑1 (MQ=255)
                  cGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGTGGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAtg                                                                                                                          >  1:879638/1‑140 (MQ=255)
                  cGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTca                                                                                                                                    <  2:692972/130‑1 (MQ=255)
                  cGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTca                                                                                                                                    >  1:692972/1‑130 (MQ=255)
                   gTTTATTTACTGGCATACATAAATGGTGCCTCCACATGTCGGCTGGTGATTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCGGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAtgt                                                                                                                         <  2:380107/140‑1 (MQ=255)
                      tatttaCTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGta                                                                                                                      >  1:1082299/1‑140 (MQ=255)
                      tatttaCTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGta                                                                                                                      >  1:1864582/1‑140 (MQ=255)
                          taCTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATAtt                                                                                                                  >  2:199816/1‑140 (MQ=255)
                          taCTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATAtt                                                                                                                  >  2:2107617/1‑140 (MQ=255)
                                 aTACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGTCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGt                                                                                                           <  1:912148/140‑1 (MQ=255)
                                 aTACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGt                                                                                                           <  1:517522/140‑1 (MQ=255)
                                 aTACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGt                                                                                                           <  2:2163195/140‑1 (MQ=255)
                                 aTACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGt                                                                                                           <  2:483619/140‑1 (MQ=255)
                                        aaTGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTa                                                                                                    <  2:923698/140‑1 (MQ=255)
                                        aaTGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTa                                                                                                    <  2:827064/140‑1 (MQ=255)
                                        aaTGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAAGGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTa                                                                                                    <  2:154507/140‑1 (MQ=255)
                                           ggcgCCTCCACATTTCGGCTGGTGCTTATCGGAATCGGATTTTCAATGTCGGCGCAGGCGATGACTACCCTGTTGATGATCAAAGGCCCGATTTACCGAGCATCACAAGCCAATGTGTATATCACTGGGTCAGTTTATgg                                                                                                 >  2:1846015/4‑140 (MQ=255)
                                               ccTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAt                                                                                                                           >  2:2368829/1‑110 (MQ=255)
                                                        ttCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGgcag                                                                                    <  1:20225/140‑1 (MQ=255)
                                                             cTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGt                                                                               >  1:337422/1‑140 (MQ=255)
                                                                gTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaa                                                                            >  2:222389/1‑140 (MQ=255)
                                                                gTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaa                                                                            >  2:2374898/1‑140 (MQ=255)
                                                                  gCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaaaa                                                                          <  1:2101567/140‑1 (MQ=255)
                                                                  gCTTATCGGAATCGGATTTTCAATGTCGGCGCAGGCGATGACTACCCTGTTGATGATCAAAGGCCCGATTTACCGAGCATCACAAGCCAATGTGTATATCACTGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaaaa                                                                          <  2:465318/140‑1 (MQ=255)
                                                                   cTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAAt                                                                         <  1:1591690/140‑1 (MQ=255)
                                                                            aTCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCtattat                                                                <  1:1467599/140‑1 (MQ=255)
                                                                                taTTTTCAATGTCGGCCCAGGCTCTGACTACCCTGCTGATGATTAAAGTCACAAATTACACAGCGTCACAAGCCAATGTGTATATTAAATGGTCAGTTTATGGGTAAAACTGTCCGCATGTAAAAATAGCTAttaactta                                                            >  2:1227909/2‑140 (MQ=255)
                                                                                gATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTAttatctta                                                            >  2:1640461/1‑140 (MQ=255)
                                                                                gATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTAttatctta                                                            >  2:2025510/1‑140 (MQ=255)
                                                                                           tCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATtct                                                 >  2:2275411/1‑140 (MQ=255)
                                                                                           tCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATtct                                                 >  2:1674051/1‑140 (MQ=255)
                                                                                            cGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATtctc                                                <  1:120595/140‑1 (MQ=255)
                                                                                                    gCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTAc                                        <  1:199816/140‑1 (MQ=255)
                                                                                                    gCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTAc                                        <  2:534589/140‑1 (MQ=255)
                                                                                                    gCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGACAATGTGTATATTACCGGGTCATTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTAc                                        <  2:743829/140‑1 (MQ=255)
                                                                                                              cccTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATc                              >  2:1700952/1‑140 (MQ=255)
                                                                                                              cccTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATc                              >  1:1380192/1‑140 (MQ=255)
                                                                                                              cccTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATc                              >  2:1325448/1‑140 (MQ=255)
                                                                                                              cccTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATc                              >  2:1363696/1‑140 (MQ=255)
                                                                                                               ccTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATcg                             >  2:1010167/1‑140 (MQ=255)
                                                                                                                  gctgATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCgcgc                          >  2:1132611/1‑140 (MQ=255)
                                                                                                                  gctgATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCgcgc                          >  1:278646/1‑140 (MQ=255)
                                                                                                                   ctgATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCt                         >  2:1581920/1‑140 (MQ=255)
                                                                                                                        gatTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCTCTGaaaa                    >  2:657863/1‑140 (MQ=255)
                                                                                                                        gatTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaa                    >  2:1619347/1‑140 (MQ=255)
                                                                                                                        gatTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaa                    >  2:449807/1‑140 (MQ=255)
                                                                                                                        gatTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaa                    >  2:242865/1‑140 (MQ=255)
                                                                                                                         atTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaaa                   <  1:1420413/140‑1 (MQ=255)
                                                                                                                         atTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaaa                   <  1:957280/140‑1 (MQ=255)
                                                                                                                           tAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAata                 <  2:1330447/140‑1 (MQ=255)
                                                                                                                           tAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAata                 <  2:422057/140‑1 (MQ=255)
                                                                                                                            caaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAatat                <  1:2042893/139‑1 (MQ=255)
                                                                                                                            aaaGGCCCAATTTACAGATCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAatat                <  1:1187808/140‑1 (MQ=255)
                                                                                                                            aaaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAatat                <  1:1010167/140‑1 (MQ=255)
                                                                                                                            aaaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAatat                <  2:1958370/140‑1 (MQ=255)
                                                                                                                            aaaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAatat                <  1:100648/140‑1 (MQ=255)
                                                                                                                             aaGGCCCGATTTACCGAGCATCACAAGCCAATGTGTATATCACTGGGTCAGTTGAGGGGTCAAACTGGCAGCATGTAAAAATACCTATGATGTTATCTGTCATTCTCTTATTCATGTGTTTTGTCGCGCTAAAAAatatt               >  2:2097375/1‑139 (MQ=255)
                                                                                                                             aaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaaa                   >  1:2020867/1‑136 (MQ=255)
                                                                                                                             aaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATg               >  1:2030645/1‑140 (MQ=255)
                                                                                                                               ggCCCGATTTACCGAGCATCACAAGCCAATGTGTATATCACTGGGTCAGTTTATGGTTCAAACTGGCAGCTTGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTCATTTGTTTTGTCGCGCTAAAAAATATGaa             <  1:2097375/140‑1 (MQ=255)
                                                                                                                               ggCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGaa             <  1:1759570/140‑1 (MQ=255)
                                                                                                                                gCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCTCTCTGAAAAATATTAat            >  2:960402/1‑140 (MQ=255)
                                                                                                                                 cccAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAata           <  1:61086/140‑1 (MQ=255)
                                                                                                                                 cccAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAata           <  1:506267/140‑1 (MQ=255)
                                                                                                                                 cccAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAata           <  1:2395713/140‑1 (MQ=255)
                                                                                                                                 cccAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAata           <  1:1286682/140‑1 (MQ=255)
                                                                                                                                    aaTTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATAc        <  1:1581920/140‑1 (MQ=255)
                                                                                                                                          cAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTTTCGGGTTTAAAAATATGAATATACAGGTaa  <  2:1864582/140‑2 (MQ=255)
                                                                                                                                          cAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTTATAAATATGAATATACAGGTaa  <  2:1082299/140‑2 (MQ=255)
                                                                                                                                          cAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTAc  <  2:2405895/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
CTCAGCTGTTGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTAC  >  NZ_CP034943/892764‑893041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: