Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 892,908 G→A 5.2% A187A (GCG→GCA EO946_RS04515 → iron ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943892,9080GA 5.2% 570.5 / 12.8 171A187A (GCG→GCAEO946_RS04515iron ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (70/92);  new base A (3/6);  total (73/98)
Fisher's exact test for biased strand distribution p-value = 7.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 172 total aligned reads displayed.

GTTGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCG  >  NZ_CP034943/892771‑893047
                                                                                                                                         |                                                                                                                                           
gTTGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTc                                                                                                                                           <  2:2319471/140‑1 (MQ=255)
 ttGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTca                                                                                                                                          >  2:150711/1‑140 (MQ=255)
 ttGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTca                                                                                                                                          <  2:1567944/140‑1 (MQ=255)
 ttGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTca                                                                                                                                          <  2:350035/140‑1 (MQ=255)
  tGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTcac                                                                                                                                         <  2:571079/140‑1 (MQ=255)
   ggACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACACTGCTGATGATTAAAGGCCCAATTTACAGAGCGTcaca                                                                                                                                        >  2:1490095/1‑140 (MQ=255)
      cTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGc                                                                                                                                     >  1:539705/1‑140 (MQ=255)
      cTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGc                                                                                                                                     <  2:2184677/140‑1 (MQ=255)
       tCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGcc                                                                                                                                    <  1:1382191/140‑1 (MQ=255)
        cATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCa                                                                                                                                   <  2:1890363/140‑1 (MQ=255)
        cATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCa                                                                                                                                   <  1:342018/140‑1 (MQ=255)
        cATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCa                                                                                                                                   <  1:315776/140‑1 (MQ=255)
        cATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCa                                                                                                                                   <  2:1152988/140‑1 (MQ=255)
        cATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCa                                                                                                                                   >  2:1286682/1‑140 (MQ=255)
        cATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCa                                                                                                                                   <  2:1630416/140‑1 (MQ=255)
        cATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCa                                                                                                                                   <  1:18614/140‑1 (MQ=255)
        cATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCa                                                                                                                                   <  2:429872/140‑1 (MQ=255)
         aTCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTcac                                                                                                                                         <  1:1862464/133‑1 (MQ=255)
         aTCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTcac                                                                                                                                         >  2:1862226/1‑133 (MQ=255)
         aTCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCaa                                                                                                                                  >  2:1420413/1‑140 (MQ=255)
          tCGTTTATTTACTGGCATACACAAATGGTGCCTCCACATTTCGGATGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACCGAGCGTCACAAGCCAAt                                                                                                                                 <  1:2277790/140‑1 (MQ=255)
          tCGTTTATTTACTGGCATACAACAATTGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTTAAGGCCCAATTTACAGAGCGTCACAAGCCAAt                                                                                                                                 <  1:23091/140‑1 (MQ=255)
          tCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAt                                                                                                                                 <  2:1221664/140‑1 (MQ=255)
          tCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAt                                                                                                                                 <  1:1986949/140‑1 (MQ=255)
          tCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAt                                                                                                                                 <  1:302251/140‑1 (MQ=255)
          tCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAt                                                                                                                                 <  1:892133/140‑1 (MQ=255)
          tCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAt                                                                                                                                 <  2:1186253/140‑1 (MQ=255)
          tCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAt                                                                                                                                 <  1:1726883/140‑1 (MQ=255)
          tCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAt                                                                                                                                 <  1:2109683/140‑1 (MQ=255)
          tCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATGTCGGCTGGTGGTTATCTGGATCGGATTTTCTATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAt                                                                                                                                 <  2:2243338/140‑1 (MQ=255)
           cGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGTGGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAtg                                                                                                                                >  1:879638/1‑140 (MQ=255)
           cGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTca                                                                                                                                          <  2:692972/130‑1 (MQ=255)
            gTTTATTTACTGGCATACATAAATGGTGCCTCCACATGTCGGCTGGTGATTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCGGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAtgt                                                                                                                               <  2:380107/140‑1 (MQ=255)
               tatttaCTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGta                                                                                                                            >  1:1082299/1‑140 (MQ=255)
               tatttaCTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGta                                                                                                                            >  1:1587921/1‑140 (MQ=255)
                   taCTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATAtt                                                                                                                        >  2:2107617/1‑140 (MQ=255)
                   taCTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATAtt                                                                                                                        >  2:199816/1‑140 (MQ=255)
                          aTACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGt                                                                                                                 <  2:2163195/140‑1 (MQ=255)
                                 aaTGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTa                                                                                                          <  2:923698/140‑1 (MQ=255)
                                 aaTGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTa                                                                                                          <  2:523533/140‑1 (MQ=255)
                                 aaTGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTa                                                                                                          <  2:827064/140‑1 (MQ=255)
                                    ggcgCCTCCACATTTCGGCTGGTGCTTATCGGAATCGGATTTTCAATGTCGGCGCAGGCGATGACTACCCTGTTGATGATCAAAGGCCCGATTTACCGAGCATCACAAGCCAATGTGTATATCACTGGGTCAGTTTATgg                                                                                                       >  2:823750/4‑140 (MQ=255)
                                        ccTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAAt                                                                                                                                 <  1:2368829/110‑1 (MQ=255)
                                        ccTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCa                                                                                                   >  1:917692/1‑140 (MQ=255)
                                        ccTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCGGATGATTAAAGGCCCAATTTACAGAGCGTCACACGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCa                                                                                                   >  2:2335516/1‑140 (MQ=255)
                                               aTTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACATAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGgc                                                                                            >  2:1327447/1‑140 (MQ=255)
                                                         gTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaa                                                                                  >  2:2374898/1‑140 (MQ=255)
                                                         gTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaa                                                                                  >  2:222389/1‑140 (MQ=255)
                                                         gTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaa                                                                                  >  2:1353826/1‑140 (MQ=255)
                                                           gCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaaaa                                                                                <  1:2101567/140‑1 (MQ=255)
                                                           gCTTATCGGAATCGGATTTTCAATGTCGGCGCAGGCGATGACTACCCTGTTGATGATCAAAGGCCCGATTTACCGAGCATCACAAGCCAATGTGTATATCACTGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaaaa                                                                                <  2:465318/140‑1 (MQ=255)
                                                            cTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAAt                                                                               <  1:1357386/140‑1 (MQ=255)
                                                                     aTCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCtattat                                                                      <  1:2132701/140‑1 (MQ=255)
                                                                     aTCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCtattat                                                                      <  1:1467599/140‑1 (MQ=255)
                                                                         gATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTAttatctta                                                                  >  2:2025510/1‑140 (MQ=255)
                                                                                    tCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATtct                                                       >  2:2275411/1‑140 (MQ=255)
                                                                                    tCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATtct                                                       >  2:1674051/1‑140 (MQ=255)
                                                                                     cGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATtctc                                                      <  1:1233867/140‑1 (MQ=255)
                                                                                             gCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTAc                                              <  2:534589/140‑1 (MQ=255)
                                                                                             gCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTAc                                              <  1:199816/140‑1 (MQ=255)
                                                                                             gCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGACAATGTGTATATTACCGGGTCATTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTAc                                              <  2:743829/140‑1 (MQ=255)
                                                                                                       cccTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATc                                    >  2:1700952/1‑140 (MQ=255)
                                                                                                       cccTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATc                                    >  2:1325448/1‑140 (MQ=255)
                                                                                                        ccTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATcg                                   >  1:2196244/1‑140 (MQ=255)
                                                                                                        ccTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATcg                                   >  2:1010167/1‑140 (MQ=255)
                                                                                                           gctgATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCgcgc                                >  2:1132611/1‑140 (MQ=255)
                                                                                                           gctgATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCgcgc                                >  1:278646/1‑140 (MQ=255)
                                                                                                           gctgATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCgcgc                                >  2:1778036/1‑140 (MQ=255)
                                                                                                            ctgATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCt                               >  2:1581920/1‑140 (MQ=255)
                                                                                                                 gatTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCTCTGaaaa                          >  2:657863/1‑140 (MQ=255)
                                                                                                                 gatTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaa                          >  2:449807/1‑140 (MQ=255)
                                                                                                                 gatTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaa                          >  2:657818/1‑140 (MQ=255)
                                                                                                                 gatTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCAGTCTCTTATTTACTTGTTTTATCGCGCTGaaaa                          >  2:1015191/1‑140 (MQ=255)
                                                                                                                  atTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaaa                         <  1:1420413/140‑1 (MQ=255)
                                                                                                                  atTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaaa                         <  1:957280/140‑1 (MQ=255)
                                                                                                                    tAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAata                       <  2:422057/140‑1 (MQ=255)
                                                                                                                     caaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAatat                      <  1:2042893/139‑1 (MQ=255)
                                                                                                                     aaaGGCCCGATTTACCGAGCATCACAAGCCAATGTGTATATCACTGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTCATTTGTTTTGTCGCGCTAAAAAatat                      <  1:1846015/140‑1 (MQ=255)
                                                                                                                     aaaGGCCCGATTTACCGAGCATCACAAGCCAATGTGTATATCACTGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTCATTTGTTTTGTCGCGCTAAAAAatat                      <  1:823750/140‑1 (MQ=255)
                                                                                                                     aaaGGCCCAATTTACAGATCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAatat                      <  1:1187808/140‑1 (MQ=255)
                                                                                                                     aaaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAatat                      <  1:1010167/140‑1 (MQ=255)
                                                                                                                     aaaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAatat                      <  1:100648/140‑1 (MQ=255)
                                                                                                                     aaaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAatat                      <  1:2041651/140‑1 (MQ=255)
                                                                                                                      aaGGCCCGATTTACCGAGCATCACAAGCCAATGTGTATATCACTGGGTCAGTTGAGGGGTCAAACTGGCAGCATGTAAAAATACCTATGATGTTATCTGTCATTCTCTTATTCATGTGTTTTGTCGCGCTAAAAAatatt                     >  2:2097375/1‑139 (MQ=255)
                                                                                                                      aaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaaa                         >  1:2020867/1‑136 (MQ=255)
                                                                                                                      aaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATg                     >  1:2030625/1‑140 (MQ=255)
                                                                                                                         gCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCTCTCTGAAAAATATTAat                  >  2:960402/1‑140 (MQ=255)
                                                                                                                          cccAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAata                 <  1:61086/140‑1 (MQ=255)
                                                                                                                          cccAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAata                 <  1:506268/140‑1 (MQ=255)
                                                                                                                            aaaTTTACAGAGCGTCACACGCAAATTTGTTTTTTCCCGGTTCATTTTATGGGTAAAACGGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAAtata               <  2:337422/139‑1 (MQ=255)
                                                                                                                             aaTTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATAc              <  1:1581920/140‑1 (MQ=255)
                                                                                                                                   cAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTTTCGGGTTTAAAAATATGAATATACAGGTaa        <  2:1864582/140‑2 (MQ=255)
                                                                                                                                   cAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTTTCGATATTATAAATATTAATATACAGGTAc        <  2:1587921/140‑1 (MQ=255)
                                                                                                                                   cAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTAc        <  2:2405895/140‑1 (MQ=255)
                                                                                                                                        cGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTCTTTACTTGTTTTATCGAGCTGAAAAATATTAATATACAGGTACTGGGc   >  1:159974/1‑140 (MQ=255)
                                                                                                                                        cGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGc   >  1:214323/1‑140 (MQ=255)
                                                                                                                                        cGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGc   >  1:206810/1‑140 (MQ=255)
                                                                                                                                        cGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGc   >  1:392074/1‑140 (MQ=255)
                                                                                                                                        cGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGc   >  1:442282/1‑140 (MQ=255)
                                                                                                                                        cGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCCTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGc   >  1:1164990/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                           
GTTGGACTCATCGTTTATTTACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCG  >  NZ_CP034943/892771‑893047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: