Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 892,929 T→C 6.7% I194I (ATT→ATC EO946_RS04515 → iron ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943892,9290TC 6.7% 422.7 / 14.3 137I194I (ATT→ATCEO946_RS04515iron ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (59/68);  new base C (3/6);  total (63/74)
Fisher's exact test for biased strand distribution p-value = 5.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

Only 100 of 139 total aligned reads displayed.

TACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGAAGATATTGCGGCGGGTGC  >  NZ_CP034943/892790‑893066
                                                                                                                                           |                                                                                                                                         
taCTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATAtt                                                                                                                                           >  2:2107617/1‑140 (MQ=255)
       aTACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGt                                                                                                                                    <  1:517522/140‑1 (MQ=255)
       aTACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGt                                                                                                                                    <  2:483619/140‑1 (MQ=255)
              aaTGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTa                                                                                                                             <  2:923698/140‑1 (MQ=255)
              aaTGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTa                                                                                                                             <  2:827064/140‑1 (MQ=255)
              aaTGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTa                                                                                                                             <  2:117513/140‑1 (MQ=255)
              aaTGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTa                                                                                                                             <  2:523533/140‑1 (MQ=255)
              aaTGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAAGGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTa                                                                                                                             <  2:154507/140‑1 (MQ=255)
                 ggcgCCTCCACATTTCGGCTGGTGCTTATCGGAATCGGATTTTCAATGTCGGCGCAGGCGATGACTACCCTGTTGATGATCAAAGGCCCGATTTACCGAGCATCACAAGCCAATGTGTATATCACTGGGTCAGTTTATgg                                                                                                                          >  2:1846015/4‑140 (MQ=255)
                 ggcgCCTCCACATTTCGGCTGGTGCTTATCGGAATCGGATTTTCAATGTCGGCGCAGGCGATGACTACCCTGTTGATGATCAAAGGCCCGATTTACCGAGCATCACAAGCCAATGTGTATATCACTGGGTCAGTTTATgg                                                                                                                          >  2:823750/4‑140 (MQ=255)
                     ccTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCa                                                                                                                      >  1:917692/1‑140 (MQ=255)
                     ccTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCa                                                                                                                      >  1:2013975/1‑140 (MQ=255)
                     ccTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCGGATGATTAAAGGCCCAATTTACAGAGCGTCACACGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCa                                                                                                                      >  2:2335516/1‑140 (MQ=255)
                     ccTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTAACCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCa                                                                                                                      >  2:1147592/1‑140 (MQ=255)
                            aTTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACATAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGgc                                                                                                               >  2:1327447/1‑140 (MQ=255)
                              ttCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGgcag                                                                                                             <  1:20225/140‑1 (MQ=255)
                                   cTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGt                                                                                                        >  1:337422/1‑140 (MQ=255)
                                      gTGCTTATCGGGATCGGATTTTCAATGTCGGGGCAGGCTCTGACTACCCTGCTGATGAGTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATTGGTCAAACTGGCAGCATGTaaa                                                                                                     >  2:2179431/1‑140 (MQ=255)
                                      gTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaa                                                                                                     >  2:2374898/1‑140 (MQ=255)
                                      gTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaa                                                                                                     >  2:222389/1‑140 (MQ=255)
                                        gCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaaaa                                                                                                   <  2:2071014/140‑1 (MQ=255)
                                        gCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaaaa                                                                                                   <  2:364940/140‑1 (MQ=255)
                                        gCTTATCGGCATCGGATTTTCAATGTCGGCGCAGGCGATGACTACCCTGTTGATGATCAAAGGCCCGATTTACCGAGCATCACAAGCCAATGTGTATATCACTGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaaaa                                                                                                   <  2:2151325/140‑1 (MQ=255)
                                        gCTTATCGGAATCGGATTTTCAATGTCGGCGCAGGCGATGACTACCCTGTTGATGATCAAAGGCCCGATTTACCGAGCATCACAAGCCAATGTGTATATCACTGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaaaa                                                                                                   <  2:465318/140‑1 (MQ=255)
                                                  aTCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCtattat                                                                                         <  1:1011359/140‑1 (MQ=255)
                                                  aTCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCtattat                                                                                         <  1:2132701/140‑1 (MQ=255)
                                                      gATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAATCTGGCAGCATGTAAAAATAGCTAttatctta                                                                                     >  2:546966/1‑140 (MQ=255)
                                                      gATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTAttatctta                                                                                     >  2:1640461/1‑140 (MQ=255)
                                                      gATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTAttatctta                                                                                     >  2:2025510/1‑140 (MQ=255)
                                                                 tCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATtct                                                                          >  2:1674051/1‑140 (MQ=255)
                                                                 tCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATtct                                                                          >  2:2275411/1‑140 (MQ=255)
                                                                  cGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATtctc                                                                         <  1:1233867/140‑1 (MQ=255)
                                                                          gCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGACAATGTGTATATTACCGGGTCATTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTAc                                                                 <  2:743829/140‑1 (MQ=255)
                                                                                    cccTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATc                                                       >  2:1325448/1‑140 (MQ=255)
                                                                                    cccTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATc                                                       >  1:1380192/1‑140 (MQ=255)
                                                                                    cccTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATc                                                       >  2:1700952/1‑140 (MQ=255)
                                                                                    cccTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATc                                                       >  2:1363696/1‑140 (MQ=255)
                                                                                      ctgctgATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATcgc                                                     >  2:1568803/1‑140 (MQ=255)
                                                                                        gctgATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCgcgc                                                   >  2:1778036/1‑140 (MQ=255)
                                                                                         ctgATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCt                                                  >  2:1581920/1‑140 (MQ=255)
                                                                                              gatTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaa                                             >  2:1619347/1‑140 (MQ=255)
                                                                                              gatTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaa                                             >  2:242865/1‑140 (MQ=255)
                                                                                              gatTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaa                                             >  2:657818/1‑140 (MQ=255)
                                                                                              gatTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaa                                             >  2:449807/1‑140 (MQ=255)
                                                                                              gatTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCAGTCTCTTATTTACTTGTTTTATCGCGCTGaaaa                                             >  2:1015191/1‑140 (MQ=255)
                                                                                               atTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaaa                                            <  1:1420413/140‑1 (MQ=255)
                                                                                               atTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaaa                                            <  1:957280/140‑1 (MQ=255)
                                                                                                 tAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAata                                          <  2:422057/140‑1 (MQ=255)
                                                                                                 tAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAata                                          <  2:1330447/140‑1 (MQ=255)
                                                                                                  caaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAatat                                         <  1:2042893/139‑1 (MQ=255)
                                                                                                  aaaGGCCCGATTTACCGAGCATCACAAGCCAATGTGTATATCACTGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTCATTTGTTTTGTCGCGCTAAAAAatat                                         <  1:1846015/140‑1 (MQ=255)
                                                                                                  aaaGGCCCGATTTACCGAGCATCACAAGCCAATGTGTATATCACTGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTCATTTGTTTTGTCGCGCTAAAAAatat                                         <  1:823750/140‑1 (MQ=255)
                                                                                                  aaaGGCCCAATTTACAGATCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAatat                                         <  1:1187808/140‑1 (MQ=255)
                                                                                                  aaaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAatat                                         <  1:2041651/140‑1 (MQ=255)
                                                                                                  aaaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAatat                                         <  1:100648/140‑1 (MQ=255)
                                                                                                  aaaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAatat                                         <  1:1010167/140‑1 (MQ=255)
                                                                                                   aaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaaa                                            >  1:2020867/1‑136 (MQ=255)
                                                                                                   aaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaaa                                            <  2:2020867/136‑1 (MQ=255)
                                                                                                   aaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATg                                        >  1:2030645/1‑140 (MQ=255)
                                                                                                   aaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGGTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATg                                        >  2:2267900/1‑140 (MQ=255)
                                                                                                     ggCCCGATTTACCGAGCATCACAAGCCAATGTGTATATCACTGGGTCAGTTTATGGTTCAAACTGGCAGCTTGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTCATTTGTTTTGTCGCGCTAAAAAATATGaa                                      <  1:2097375/140‑1 (MQ=255)
                                                                                                     ggCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGaa                                      <  1:1759570/140‑1 (MQ=255)
                                                                                                      gCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCTCTCTGAAAAATATTAat                                     >  2:960402/1‑140 (MQ=255)
                                                                                                       cccAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAata                                    <  1:1286682/140‑1 (MQ=255)
                                                                                                       cccAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAata                                    <  1:2395713/140‑1 (MQ=255)
                                                                                                       cccAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAata                                    <  1:61086/140‑1 (MQ=255)
                                                                                                       cccAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAata                                    <  1:506268/140‑1 (MQ=255)
                                                                                                         aaaTTTACAGAGCGTCACACGCAAATTTGTTTTTTCCCGGTTCATTTTATGGGTAAAACGGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAAtata                                  <  2:337422/139‑1 (MQ=255)
                                                                                                          aaTTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATAc                                 <  1:1581920/140‑1 (MQ=255)
                                                                                                                cAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTTTCGGGTTTAAAAATATGAATATACAGGTaa                           <  2:1864582/140‑2 (MQ=255)
                                                                                                                cAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTTATAAATATGAATATACAGGTaa                           <  2:1082299/140‑2 (MQ=255)
                                                                                                                cAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTAc                           <  2:1293242/140‑1 (MQ=255)
                                                                                                                cAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTAc                           <  1:2107617/140‑1 (MQ=255)
                                                                                                                     cGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTCTGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGc                      >  1:2193364/1‑140 (MQ=255)
                                                                                                                     cGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTCTTTACTTGTTTTATCGAGCTGAAAAATATTAATATACAGGTACTGGGc                      >  1:159974/1‑140 (MQ=255)
                                                                                                                     cGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGc                      >  1:1126925/1‑140 (MQ=255)
                                                                                                                     cGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGc                      >  1:392074/1‑140 (MQ=255)
                                                                                                                     cGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGc                      >  1:442282/1‑140 (MQ=255)
                                                                                                                     cGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGc                      >  1:871452/1‑140 (MQ=255)
                                                                                                                     cGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCCTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGc                      >  1:1164990/1‑140 (MQ=255)
                                                                                                                      gTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCg                     >  1:1445547/1‑140 (MQ=255)
                                                                                                                       tCACAAGCCAATGTTTATATTACCGGGTCAGTTTATGGGTCAACCTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGa                    <  1:2088248/140‑1 (MQ=255)
                                                                                                                       tCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGa                    <  2:2196244/140‑1 (MQ=255)
                                                                                                                       tCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGa                    <  1:984581/140‑1 (MQ=255)
                                                                                                                             gCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGAAGatat              >  2:1150674/1‑140 (MQ=255)
                                                                                                                                aaTGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGAAGATCTTgc           >  1:1580084/1‑140 (MQ=255)
                                                                                                                                aaTGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGAAGATATTgc           >  1:353547/1‑140 (MQ=255)
                                                                                                                                  tgtgTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTCTTATCTTATCTGTCATtctc                                                                         >  2:217728/1‑76 (MQ=255)
                                                                                                                                  tgtgTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATtctc                                                                         <  1:217728/76‑1 (MQ=255)
                                                                                                                                  tgtgTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAatat                                         >  1:1838964/1‑108 (MQ=255)
                                                                                                                                    tgtATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGACAAATATGAATATACAGGTACTGGGCGAAGATATTgcggcg       <  1:425853/140‑1 (MQ=255)
                                                                                                                                    tgtATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATCTACAGGTACTGGGCGAAGATATTgcggcg       <  1:2105358/140‑1 (MQ=255)
                                                                                                                                    tgtATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGAAGATATTgcggcg       <  1:273604/140‑1 (MQ=255)
                                                                                                                                       atatTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGAAGATATTGCGGCGGGt    <  2:199380/140‑1 (MQ=255)
                                                                                                                                       atatTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGAAGATATTGCGGCGGGt    >  2:1919761/1‑140 (MQ=255)
                                                                                                                                       atatTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGAAGATATTGCGGCGGGt    <  2:199481/140‑1 (MQ=255)
                                                                                                                                       atatTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGAAGATATTGCGGCGGGt    <  1:2382100/140‑1 (MQ=255)
                                                                                                                                       atatTACCGCGTCAGTTTATGGGTCAAACTGTCAGCATGTAAAAATAGCTATTATCGTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGAAGATATTGCGGCGGGt    <  2:1252423/140‑1 (MQ=255)
                                                                                                                                        tatTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGAAGATATTGCGGCGGGTg   >  1:1721522/1‑140 (MQ=255)
                                                                                                                                         atTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGAAGATATTGCGGCGGGTGc  <  2:1380192/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
TACTGGCATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGAAGATATTGCGGCGGGTGC  >  NZ_CP034943/892790‑893066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: