Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 892,932 C→T 6.5% T195T (ACC→ACT EO946_RS04515 → iron ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943892,9320CT 6.5% 436.5 / 13.5 138T195T (ACC→ACTEO946_RS04515iron ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (58/69);  new base T (3/6);  total (63/75)
Fisher's exact test for biased strand distribution p-value = 7.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 142 total aligned reads displayed.

ATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGAAGATATTGCGGCGGGTGCGGGAA  >  NZ_CP034943/892797‑893071
                                                                                                                                       |                                                                                                                                           
aTACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGTCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGt                                                                                                                                         <  1:912148/140‑1 (MQ=255)
aTACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGt                                                                                                                                         <  2:458781/140‑1 (MQ=255)
aTACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGt                                                                                                                                         <  1:517522/140‑1 (MQ=255)
       aaTGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTa                                                                                                                                  <  2:523533/140‑1 (MQ=255)
       aaTGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTa                                                                                                                                  <  2:923698/140‑1 (MQ=255)
       aaTGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTa                                                                                                                                  <  2:827064/140‑1 (MQ=255)
       aaTGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTa                                                                                                                                  <  2:117513/140‑1 (MQ=255)
       aaTGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTa                                                                                                                                  <  2:2291609/140‑1 (MQ=255)
       aaTGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAAGGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTa                                                                                                                                  <  2:154507/140‑1 (MQ=255)
          ggcgCCTCCACATTTCGGCTGGTGCTTATCGGAATCGGATTTTCAATGTCGGCGCAGGCGATGACTACCCTGTTGATGATCAAAGGCCCGATTTACCGAGCATCACAAGCCAATGTGTATATCACTGGGTCAGTTTATgg                                                                                                                               >  2:823750/4‑140 (MQ=255)
          ggcgCCTCCACATTTCGGCTGGTGCTTATCGGAATCGGATTTTCAATGTCGGCGCAGGCGATGACTACCCTGTTGATGATCAAAGGCCCGATTTACCGAGCATCACAAGCCAATGTGTATATCACTGGGTCAGTTTATgg                                                                                                                               >  2:1846015/4‑140 (MQ=255)
           gTGCCTCCACATTTCGGCTGGTGATTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCAGACAACACTGCTGATGATTAAAGGCCCAATTAACAGAGAGTCACAATCCCCAGTGTAAAATACCGGGTCAGTTTATggg                                                                                                                              >  2:169759/1‑140 (MQ=255)
              ccTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCa                                                                                                                           >  1:2013975/1‑140 (MQ=255)
              ccTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCa                                                                                                                           >  1:917692/1‑140 (MQ=255)
              ccTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCGGATGATTAAAGGCCCAATTTACAGAGCGTCACACGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCa                                                                                                                           >  2:2335516/1‑140 (MQ=255)
                     aTTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACATAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGgc                                                                                                                    >  2:1327447/1‑140 (MQ=255)
                               gTGCTTATCGGGATCGGATTTTCAATGTCGGGGCAGGCTCTGACTACCCTGCTGATGAGTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATTGGTCAAACTGGCAGCATGTaaa                                                                                                          >  2:2179431/1‑140 (MQ=255)
                               gTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaa                                                                                                          >  2:222389/1‑140 (MQ=255)
                               gTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaa                                                                                                          >  2:1353826/1‑140 (MQ=255)
                                 tcTTATCGGAATCGGATTTTCAATGTCGGCGCAGGCGATGACTACCCTGTTGATGATCAAAGGCCCGATTTACCGAGCATCACAAGCCAATGTGTATATCACTGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaaaa                                                                                                        <  2:2213790/139‑1 (MQ=255)
                                 gCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaaaa                                                                                                        <  2:2071014/140‑1 (MQ=255)
                                 gCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaaaa                                                                                                        <  2:364940/140‑1 (MQ=255)
                                 gCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaaaa                                                                                                        <  1:2101567/140‑1 (MQ=255)
                                 gCTTATCGGCATCGGATTTTCAATGTCGGCGCAGGCGATGACTACCCTGTTGATGATCAAAGGCCCGATTTACCGAGCATCACAAGCCAATGTGTATATCACTGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaaaa                                                                                                        <  2:2151325/140‑1 (MQ=255)
                                 gCTTATCGGAATCGGATTTTCAATGTCGGCGCAGGCGATGACTACCCTGTTGATGATCAAAGGCCCGATTTACCGAGCATCACAAGCCAATGTGTATATCACTGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaaaa                                                                                                        <  2:465318/140‑1 (MQ=255)
                                 gCTGATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTaaaaa                                                                                                        <  2:1480603/140‑1 (MQ=255)
                                  cTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAAt                                                                                                       <  1:1591690/140‑1 (MQ=255)
                                           aTCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCtattat                                                                                              <  1:1011359/140‑1 (MQ=255)
                                           aTCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCtattat                                                                                              <  1:2132701/140‑1 (MQ=255)
                                           aTCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCtattat                                                                                              <  1:1467599/140‑1 (MQ=255)
                                               taTTTTCAATGTCGGCCCAGGCTCTGACTACCCTGCTGATGATTAAAGTCACAAATTACACAGCGTCACAAGCCAATGTGTATATTAAATGGTCAGTTTATGGGTAAAACTGTCCGCATGTAAAAATAGCTAttaactta                                                                                          >  2:1227909/2‑140 (MQ=255)
                                               gATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAATCTGGCAGCATGTAAAAATAGCTAttatctta                                                                                          >  2:546966/1‑140 (MQ=255)
                                                          tCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATtct                                                                               >  2:1674051/1‑140 (MQ=255)
                                                          tCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATtct                                                                               >  2:2275411/1‑140 (MQ=255)
                                                           cGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATtctc                                                                              <  1:120595/140‑1 (MQ=255)
                                                           cGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATtctc                                                                              <  1:1233867/140‑1 (MQ=255)
                                                                   gCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTAc                                                                      <  1:199816/140‑1 (MQ=255)
                                                                   gCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTAc                                                                      <  2:534589/140‑1 (MQ=255)
                                                                             cccTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATc                                                            >  1:1380192/1‑140 (MQ=255)
                                                                              ccTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATcg                                                           >  1:2196244/1‑140 (MQ=255)
                                                                              ccTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATcg                                                           >  2:1010167/1‑140 (MQ=255)
                                                                                 gctgATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCgcgc                                                        >  2:1132611/1‑140 (MQ=255)
                                                                                 gctgATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCgcgc                                                        >  1:278646/1‑140 (MQ=255)
                                                                                 gctgATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCgcgc                                                        >  2:1778036/1‑140 (MQ=255)
                                                                                  ctgATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCt                                                       >  2:1581920/1‑140 (MQ=255)
                                                                                       gatTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCTCTGaaaa                                                  >  2:657863/1‑140 (MQ=255)
                                                                                       gatTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaa                                                  >  2:1619347/1‑140 (MQ=255)
                                                                                       gatTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaa                                                  >  2:242865/1‑140 (MQ=255)
                                                                                       gatTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaa                                                  >  2:657818/1‑140 (MQ=255)
                                                                                        atTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaaa                                                 >  2:415114/1‑140 (MQ=255)
                                                                                        atTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaaa                                                 <  1:1420413/140‑1 (MQ=255)
                                                                                          tAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAata                                               <  2:422057/140‑1 (MQ=255)
                                                                                          tAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAata                                               <  2:1330447/140‑1 (MQ=255)
                                                                                           caaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAatat                                              <  1:2042893/139‑1 (MQ=255)
                                                                                           aaaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAatat                                              <  2:1958370/140‑1 (MQ=255)
                                                                                           aaaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAatat                                              <  1:1010167/140‑1 (MQ=255)
                                                                                           aaaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAatat                                              <  1:100648/140‑1 (MQ=255)
                                                                                            aaGGCCCGATTTACCGAGCATCACAAGCCAATGTGTATATCACTGGGTCAGTTGAGGGGTCAAACTGGCAGCATGTAAAAATACCTATGATGTTATCTGTCATTCTCTTATTCATGTGTTTTGTCGCGCTAAAAAatatt                                             >  2:2097375/1‑139 (MQ=255)
                                                                                            aaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaaa                                                 <  2:2020867/136‑1 (MQ=255)
                                                                                            aaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGaaaaa                                                 >  1:2020867/1‑136 (MQ=255)
                                                                                            aaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATg                                             >  1:2030645/1‑140 (MQ=255)
                                                                                            aaGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATg                                             >  1:2030625/1‑140 (MQ=255)
                                                                                              ggCCCGATTTACCGAGCATCACAAGCCAATGTGTATATCACTGGGTCAGTTTATGGTTCAAACTGGCAGCTTGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTCATTTGTTTTGTCGCGCTAAAAAATATGaa                                           <  1:2097375/140‑1 (MQ=255)
                                                                                               gCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCTCTCTGAAAAATATTAat                                          >  2:960402/1‑140 (MQ=255)
                                                                                                cccAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAata                                         <  1:2395713/140‑1 (MQ=255)
                                                                                                cccAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAata                                         <  1:1286682/140‑1 (MQ=255)
                                                                                                cccAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAata                                         <  1:506267/140‑1 (MQ=255)
                                                                                                cccAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAata                                         <  1:61086/140‑1 (MQ=255)
                                                                                                         cAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTTTCGATATTATAAATATTAATATACAGGTAc                                <  2:1587921/140‑1 (MQ=255)
                                                                                                         cAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTTATAAATATGAATATACAGGTaa                                <  2:1082299/140‑2 (MQ=255)
                                                                                                         cAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTAc                                <  2:1293242/140‑1 (MQ=255)
                                                                                                         cAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTAc                                <  2:2405895/140‑1 (MQ=255)
                                                                                                              cGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTCTGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGc                           >  1:2193364/1‑140 (MQ=255)
                                                                                                              cGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTCTTTACTTGTTTTATCGAGCTGAAAAATATTAATATACAGGTACTGGGc                           >  1:159974/1‑140 (MQ=255)
                                                                                                              cGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGc                           >  1:1126925/1‑140 (MQ=255)
                                                                                                              cGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGc                           >  1:206810/1‑140 (MQ=255)
                                                                                                              cGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGc                           >  1:214323/1‑140 (MQ=255)
                                                                                                              cGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGc                           >  1:442282/1‑140 (MQ=255)
                                                                                                              cGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGc                           >  1:871452/1‑140 (MQ=255)
                                                                                                              cGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCCTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGc                           >  1:1164990/1‑140 (MQ=255)
                                                                                                                tCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGa                         <  1:984581/140‑1 (MQ=255)
                                                                                                                tCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGa                         <  1:358563/140‑1 (MQ=255)
                                                                                                                tCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCGTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGa                         <  1:682258/140‑1 (MQ=255)
                                                                                                                 aacaAGCCAATGTGTATATAACATTGACATTTAATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCTCGCTGAAAAATATGAATATACAGGTAATGGGCGaa                        >  2:1318642/2‑140 (MQ=255)
                                                                                                                      gCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGAAGatat                   >  2:1150674/1‑140 (MQ=255)
                                                                                                                         aaTGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGAAGATCTTgc                >  1:1580084/1‑140 (MQ=255)
                                                                                                                         aaTGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGAAGATATTgc                >  1:353547/1‑140 (MQ=255)
                                                                                                                           tgtgTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTCTTATCTTATCTGTCATtctc                                                                              >  2:217728/1‑76 (MQ=255)
                                                                                                                           tgtgTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAatat                                              >  1:1838964/1‑108 (MQ=255)
                                                                                                                           tgtgTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAatat                                              <  2:1838964/108‑1 (MQ=255)
                                                                                                                             tgtATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGACAAATATGAATATACAGGTACTGGGCGAAGATATTgcggcg            <  1:425853/140‑1 (MQ=255)
                                                                                                                             tgtATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATCTACAGGTACTGGGCGAAGATATTgcggcg            <  1:2105358/140‑1 (MQ=255)
                                                                                                                             tgtATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGAAGATATTgcggcg            <  1:273604/140‑1 (MQ=255)
                                                                                                                                atatTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGAAGATATTGCGGCGGGt         <  2:199481/140‑1 (MQ=255)
                                                                                                                                atatTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGAAGATATTGCGGCGGGt         >  2:1919761/1‑140 (MQ=255)
                                                                                                                                 tatTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGAAGATATTGCGGCGGGTg        >  1:178652/1‑140 (MQ=255)
                                                                                                                                 tatTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGAAGATATTGCGGCGGGTg        >  1:1721522/1‑140 (MQ=255)
                                                                                                                                  atTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGAAGATATTGCGGCGGGTGc       <  2:1380192/140‑1 (MQ=255)
                                                                                                                                       cGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGACGAAATTGCCGCGGGTGCGGGac  >  2:1549936/1‑139 (MQ=255)
                                                                                                                                       cGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGAAGATATTGCGGCGGGTGCGGGaa  >  1:667642/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                           
ATACAAAAATGGTGCCTCCACATTTCGGCTGGTGCTTATCGGGATCGGATTTTCAATGTCGGCGCAGGCTCTGACTACCCTGCTGATGATTAAAGGCCCAATTTACAGAGCGTCACAAGCCAATGTGTATATTACCGGGTCAGTTTATGGGTCAAACTGGCAGCATGTAAAAATAGCTATTATCTTATCTGTCATTCTCTTATTTACTTGTTTTATCGCGCTGAAAAATATGAATATACAGGTACTGGGCGAAGATATTGCGGCGGGTGCGGGAA  >  NZ_CP034943/892797‑893071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: