Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,013,969 C→A 5.4% D70Y (GAT→TAT)  EO946_RS05230 ← polysaccharide deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,013,9690CA 5.4% 467.1 / 10.3 147D70Y (GAT→TAT) EO946_RS05230polysaccharide deacetylase
Reads supporting (aligned to +/- strand):  ref base C (67/72);  new base A (4/4);  total (71/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 147 total aligned reads displayed.

GTCGCTTTTACATCAGCGCTTTTCAGTACGTCGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATA  >  NZ_CP034943/1013836‑1014103
                                                                                                                                     |                                                                                                                                      
 tCGCTTTTACATCAGCGCTTTTCAGTACGTCGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAg                                                                                                                                 <  1:1697198/140‑1 (MQ=255)
         aCATCAGCGCTTTTCAGTACGTCGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGttttt                                                                                                                         <  2:1816847/140‑1 (MQ=255)
               gcgcTTTTCAGTACGTCGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGt                                                                                                                                >  2:2047103/1‑127 (MQ=255)
               gcgcTTTTCAGTACGTCGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCtt                                                                                                                   <  1:1079896/140‑1 (MQ=255)
               gcgcTTTTCAGTACGTCGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCtt                                                                                                                   >  1:2024304/1‑140 (MQ=255)
               gcgcTTTTCAGTACGTCGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTAAGCtt                                                                                                                   >  1:148765/1‑140 (MQ=255)
               gcgcTTTTCAGTACGTCGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGAGGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAgggt                                                                                                                   >  1:2170841/1‑137 (MQ=255)
               gcgcTTTTCAGGACGTCGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGGCTTATCTCCCATGTTCTGCTTTGTCTGCGGTTGTTTTATCAGCAGATCGGGGGAGTGGTTTTTCTGCtt                                                                                                                   >  1:2412695/1‑140 (MQ=255)
                cgcTTTTCAGTACGTCGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTc                                                                                                                  <  1:1059233/140‑1 (MQ=255)
                cgcTTTTCAGTACGTCGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTc                                                                                                                  <  1:1566149/140‑1 (MQ=255)
                cgcTTTTCAGTACGTCGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTc                                                                                                                  <  1:1566148/140‑1 (MQ=255)
                 gcTTTTCAGTACGTCGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGCTGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCa                                                                                                                 <  1:1206831/140‑1 (MQ=255)
                 gcTTTTCAGTACGTCGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCa                                                                                                                 <  1:775796/140‑1 (MQ=255)
                  cTTTTCAGTACGTCGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAg                                                                                                                >  2:1088023/1‑140 (MQ=255)
                   ttttCAGTACGTCGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAgg                                                                                                               <  1:928495/140‑1 (MQ=255)
                          tACGTCGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTAAGCTTCAGGCTTTCCt                                                                                                        >  1:1520487/1‑140 (MQ=255)
                             gTCGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTtct                                                                                                     <  1:591517/140‑1 (MQ=255)
                             gTCGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTtct                                                                                                     <  1:2188556/140‑1 (MQ=255)
                             gTCGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTtct                                                                                                     <  1:1159742/140‑1 (MQ=255)
                             gTCGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTtct                                                                                                     <  1:1924288/140‑1 (MQ=255)
                               cGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTCAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCATCTTCAGGCTTTCCttataa                                                                                                   >  1:281955/1‑136 (MQ=255)
                               cGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTGTCa                                                                                                   >  1:619391/1‑140 (MQ=255)
                               cGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCa                                                                                                   >  2:738260/1‑140 (MQ=255)
                               cGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGATTCAGGCTTTCCTTCTCa                                                                                                   >  2:1769116/1‑140 (MQ=255)
                               cGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATAGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCa                                                                                                   >  2:807911/1‑140 (MQ=255)
                               cGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATAGGGGGAGTCGTTTTTAAGCTTCAGGCTTTACTTCTCa                                                                                                   >  2:1441114/1‑140 (MQ=255)
                               cGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCGGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTGTTTCAGCTTAAGGCTTTGCTTcaca                                                                                                   >  2:379769/1‑140 (MQ=255)
                                            tGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCGTCTCATTGCGAAGCTGGc                                                                                      >  1:1347160/1‑140 (MQ=255)
                                                taGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGtt                                                                                  <  1:2380342/140‑1 (MQ=255)
                                                taGGCTCCCGGGCCATAATCGAAGGTTAAGTAAACCGTCTGATCTCCCATGTTCAGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGtt                                                                                  <  2:2290457/140‑1 (MQ=255)
                                                  ggCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTAAGGCTTTACTTCTAATTGCGAAGCTGGCCGgttt                                                                                >  1:515720/1‑139 (MQ=255)
                                                   gCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTACTTCTCATTGCGAAGCTGGCCGGTtgt                                                                               >  1:720158/1‑140 (MQ=255)
                                                       aCGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCGTCATGCTTTCCGTCTCATTGCGAACCTGGCCGGTTgtggtg                                                                           >  1:2023549/1‑140 (MQ=255)
                                                        cGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTGCTTCTCATTGCGAAGCTGGCCGTGTGtggggg                                                                          >  1:1357430/1‑140 (MQ=255)
                                                        cGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGtggtgg                                                                          >  2:863823/1‑140 (MQ=255)
                                                        cGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCAATGCGAAGCTGGCCGTTTGtggtgg                                                                          >  1:1164092/1‑140 (MQ=255)
                                                        cGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTGTTTCGGCTTCAGGCTTTCCTTCTCATTGCGAAGCGGGCCGTTTGtggggg                                                                          >  1:1569513/1‑140 (MQ=255)
                                                        cGGGCCATCATCGAAGGTTAAGTAAACCGTCGTCGCTCCCATGTTCTGCGTTGTCTGCAGATGGTTTATCAGCAGAGCGGGGGAGTCGTTTGTCAGCTTCAGGCTTTCCGGCTCATTGCGAAGCTGGCCGTTTGtggtgg                                                                          >  2:1169811/1‑140 (MQ=255)
                                                             catcatCGAAGGTTAAGTCAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTGTTTCTGCGTGAGGGGTTGCTTCTCATTGCGACGCTGGCCGTGTGGGGGGGCCGTg                                                                     >  2:2217545/1‑140 (MQ=255)
                                                             catcatCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTg                                                                     <  2:257850/140‑1 (MQ=255)
                                                             catcatCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCAGTGCGAAGCTGGCCGTTTGTGGTGGCAGTg                                                                     <  2:429882/140‑1 (MQ=255)
                                                                                aaCCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATg                                                  <  1:2106712/140‑1 (MQ=255)
                                                                                   cGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGc                                               <  2:826612/140‑1 (MQ=255)
                                                                                   cGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGc                                               <  2:1984708/140‑1 (MQ=255)
                                                                                   cGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGc                                               <  2:1627430/140‑1 (MQ=255)
                                                                                     tCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATg                                                  <  1:471478/135‑1 (MQ=255)
                                                                                     tCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATg                                                  >  2:471478/1‑135 (MQ=255)
                                                                                      cTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTGAGCTTCAGGCTTTCCTTGTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGGGACGTCCTTTGGTTTCGATGAGCTGt                                            >  1:808369/1‑140 (MQ=255)
                                                                                      cTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTAAGCTTCAGGCGTTCCGTCTCATTGGGAAGCTGGCCGTTTGTGGGGGCAGTGGCGTGCTTTTGTTTCGAGGAGCTGt                                            >  1:808411/1‑140 (MQ=255)
                                                                                       ttATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCGCATTGCGAAGCTGGCCGTTTGTGGTGGCCGTGACTTCCTTTGCTTTCGATGAGCTGtt                                           >  2:1052479/1‑140 (MQ=255)
                                                                                       ttATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCATAGCATTGGGCACCTGGCCGTTTGTGGTGGCCGTGACTTCCTTTGCTTTAGATTAggggtt                                           >  2:158536/1‑135 (MQ=255)
                                                                                       ttATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTACTTCTCATTTCGAAGCTGGCCGTTTTTGGTGGCAGTGACTTCCTTTGCTTTAGATTAGCTGtt                                           >  2:2199789/1‑140 (MQ=255)
                                                                                          tctcCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGa                                        <  1:357144/140‑1 (MQ=255)
                                                                                          tctcCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGa                                        <  1:1993991/140‑1 (MQ=255)
                                                                                          tctcCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATAGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGa                                        <  1:1441114/140‑1 (MQ=255)
                                                                                          tctcCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATAGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGa                                        <  1:2224186/140‑1 (MQ=255)
                                                                                          tcgccccTGTTCTGCTTTGTCGGCAGATGTTGTCTCAGCAGATAGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGa                                        <  1:1243493/137‑1 (MQ=255)
                                                                                           ctcCCATGTTCTGCTTTGTCTGCAGATGTTTTCTCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGaa                                       <  1:2084934/140‑1 (MQ=255)
                                                                                           ctcCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGaa                                       <  2:1803065/140‑1 (MQ=255)
                                                                                           ctcCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGaa                                       <  2:1965084/140‑1 (MQ=255)
                                                                                           ctcCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGaa                                       <  1:1636011/140‑1 (MQ=255)
                                                                                           ctcCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGaa                                       <  2:2221919/140‑1 (MQ=255)
                                                                                           ctcCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGaa                                       <  2:2429071/140‑1 (MQ=255)
                                                                                           ctcCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGaa                                       <  2:1366120/140‑1 (MQ=255)
                                                                                           ctcCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGaa                                       <  1:1980534/140‑1 (MQ=255)
                                                                                           ctcCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGaa                                       <  1:398472/140‑1 (MQ=255)
                                                                                           ctcCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGaa                                       <  1:689723/140‑1 (MQ=255)
                                                                                               cATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGtt                                                                                  >  2:1209112/1‑93 (MQ=255)
                                                                                               cATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGtt                                                                                  <  1:1971351/93‑1 (MQ=255)
                                                                                               cATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGtt                                                                                  >  2:1971351/1‑93 (MQ=255)
                                                                                               cATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCACGCTTTCCATCTAATTGCGAAACTGGCCGTTTGTGGTGGCATTGACATCAATTTCATTAGATGAGCTGTTTTAATAcc                                   >  1:1280945/1‑140 (MQ=255)
                                                                                                aTGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATAccc                                  <  2:1701738/140‑1 (MQ=255)
                                                                                                 tgttgtGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGGTTGAGGCTTTCCGTCTCATTCCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTTGTTTCGGTGAGCTGGTGGAAAACCCt                                 >  2:442159/1‑140 (MQ=255)
                                                                                                 tGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTGCACATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGACCTGTTTGACTACCCt                                 >  2:651824/1‑140 (MQ=255)
                                                                                                     cTGGTTTGTCTGCAGATGTTGTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGaa                             <  2:1460013/140‑1 (MQ=255)
                                                                                                      tGCTTTGTCTGCAGATTTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGGCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAAt                            <  1:624285/140‑1 (MQ=255)
                                                                                                      tGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTGAGCTTCAGGCTTTCCTTCTCATTGCGAAGCAGGCCGTTTGTGGTGGGAGTGACTTACTTTTCTGTCGTTGTGCTGTTTTAATAaccaaagag                            >  1:244912/1‑134 (MQ=255)
                                                                                                      tGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAAt                            <  1:1759032/140‑1 (MQ=255)
                                                                                                      tGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCGTAAGGCTTTCCTTCTCATTTCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTTCTTTCGATGAGCTGTTGGAATAACCTAGAAt                            >  1:1046409/1‑140 (MQ=255)
                                                                                                           tGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCa                       <  1:1594637/140‑1 (MQ=255)
                                                                                                           tGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCa                       <  1:852430/140‑1 (MQ=255)
                                                                                                           tGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCa                       <  1:1584535/140‑1 (MQ=255)
                                                                                                           tGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCGTTCCTTCTCATTTGGAAGCTGGCCGTTTTTTGTGGCAGTGACATCCTTTGGTTTCGCTGAGCGGTTTGCATAACCGAGAATCCCCa                       >  1:939085/1‑140 (MQ=255)
                                                                                                             tCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTATCATTGCGAAGCTGGCCGTTTTTGGTGGCAGTGACTTACTTTGCTTTAGATGATCTGTTTGGATACCCACGAAACACCaaa                     >  1:442011/1‑138 (MQ=255)
                                                                                                             tCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTACTTCTCATTTCGAAGCAGGCCGTTTGTGGTGGCAGTGACATCCTTTTCGTTAGATGAGCTGTTTGAATACACGAGAAACACCaaa                     >  1:854267/1‑138 (MQ=255)
                                                                                                             tCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTACTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTACATTTCTTTGGATTAGCTGTTTTAATAACCAATAATAACCaaa                     >  1:1935605/1‑138 (MQ=255)
                                                                                                             tCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTACTTATCAATTCGAAGCTGGCCGGTTTTGGTGGCCGTTACAGACTTTTCTTTAGATGAGCTGTTTGAAAAACCTATAATCAcccaa                     >  1:442010/1‑137 (MQ=255)
                                                                                                             tCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCGTCAGGCTTTCCTTCTCATTGCGACGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGGTTTCGCTGAGCTGTTTGAATACCCGAGAATAACCATa                     >  1:2276492/1‑140 (MQ=255)
                                                                                                               tGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGGGAAGCTGGCCGGTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTTGTCTACCCTATAATCAACATaaa                   >  2:1302572/1‑139 (MQ=255)
                                                                                                                gCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGc                  <  2:163744/140‑1 (MQ=255)
                                                                                                                ccAGACGTTTTACCAGCAGATCGGGGGAGTCGAGTTTCACCCTCAGCCTGCCCTACACATCGCGACCCGCCCCCTCTGCGCTGGCACTCACTTCCCCTGCCACCGATCACCTCATGGACTACCCTACAATCACCATaacc                  <  2:13755/139‑3 (MQ=255)
                                                                                                                  aGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCGTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTAGATGAGCTGTTGGAATACCCTAGAATCACCAAAAGCaa                >  2:1660950/1‑140 (MQ=255)
                                                                                                                       ttttATCAGCAGATCGGGGGAGTCGTTTTTACGCTTGAGGCTTTCCTTCTCATTGCGAAGCTGGGCGGTTGTGGTGGCAGTGTATTCCATTGGTTTGGATGATCTGTTGTGATACCCAACAATCAACAAAAGCAAAaaca           >  1:749314/1‑140 (MQ=255)
                                                                                                                         ttATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAagcagc         <  1:344301/140‑1 (MQ=255)
                                                                                                                         ttATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAagcagc         <  1:1438911/140‑1 (MQ=255)
                                                                                                                         ttATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAagcagc         <  2:856551/140‑1 (MQ=255)
                                                                                                                          tATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAgcagca        <  1:348584/140‑1 (MQ=255)
                                                                                                                                cagATCGGGGGAGTCGTTTTTGACCTTCAGGCTTTACTTCTAAATGCGAAGCATGCCGTTTTTTGTTGCAATGACATCCTTTTCTTTAGATGATGTGTTTGGATAACCTAGTATCACCAAAAAAAAAAGCACCACAAATa  >  1:2270058/1‑140 (MQ=255)
                                                                                                                                cagATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCCGTGACTTCCTTTGCTTTCGATTAGCTGTTGGTATACACTATAATCACCATAAGCAAAAGCAGCACACATa  >  2:2300952/1‑140 (MQ=255)
                                                                                                                                cagATCGGGGGAGTCGTTTTTCAGCGTCAGGCTTTGCGTGTCATTTCGAAGCTGGCCCTTTGTGGTGGCAGTGACATGCTTTTCGTTCGATGAGATGTTGGAATAAACGAGAATAACCAAAAGCAAAAGCAGcacacaaa  >  1:1191241/1‑138 (MQ=255)
                                                                                                                                     |                                                                                                                                      
GTCGCTTTTACATCAGCGCTTTTCAGTACGTCGAGCAAGCGGTTTGTATAGGCTGACGGGCCATCATCGAAGGTTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATA  >  NZ_CP034943/1013836‑1014103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: