Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,061,263 T→C 7.1% S130S (TCT→TCC hemY → protoporphyrinogen oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,061,2630TC 7.1% 526.8 / 21.4 170S130S (TCT→TCChemYprotoporphyrinogen oxidase
Reads supporting (aligned to +/- strand):  ref base T (97/60);  new base C (6/6);  total (103/67)
Fisher's exact test for biased strand distribution p-value = 5.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.21e-01

Only 100 of 170 total aligned reads displayed.

ATTTGCTTGTCAACAATGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCA  >  NZ_CP034943/1061127‑1061399
                                                                                                                                        |                                                                                                                                        
aTTTGCTTGTCAACAATGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTggg                                                                                                                                       <  1:1717830/140‑1 (MQ=255)
      ttGTCAACAATGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCa                                                                                                                                 <  2:2252713/140‑1 (MQ=255)
       tGTCAACAATGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCaa                                                                                                                                >  1:730422/1‑140 (MQ=255)
       tGTCAACAATGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCaa                                                                                                                                >  1:1110933/1‑140 (MQ=255)
        gTCAACAATGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAg                                                                                                                               >  2:121345/1‑140 (MQ=255)
        gTCAACAATGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAg                                                                                                                               <  1:827391/140‑1 (MQ=255)
        gTCAACAATGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAg                                                                                                                               >  2:635293/1‑140 (MQ=255)
        gTCAACAATGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAg                                                                                                                               <  1:1628634/140‑1 (MQ=255)
         tCAACAATGCAACAGGTCAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAgg                                                                                                                              >  1:1812200/1‑140 (MQ=255)
               aTGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCt                                                                                                                        <  1:462377/140‑1 (MQ=255)
               aTGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCt                                                                                                                        <  1:2287569/140‑1 (MQ=255)
                tGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGACGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCGGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCtc                                                                                                                       >  2:1306992/1‑139 (MQ=255)
                tGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTa                                                                                                                       >  2:1598049/1‑140 (MQ=255)
                tGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTa                                                                                                                       >  2:1892671/1‑140 (MQ=255)
                tGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTa                                                                                                                       >  2:1001897/1‑140 (MQ=255)
                tGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTGGGTTGCACCCGGGGTGTTTTGTGTGTGTGGGACGGCAAGGGCGGCTa                                                                                                                       >  2:10975/1‑140 (MQ=255)
                tGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGAGGGGGCTACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTa                                                                                                                       >  2:508295/1‑140 (MQ=255)
                 gCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTAt                                                                                                                      <  2:280318/140‑1 (MQ=255)
                     cAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGAt                                                                                                                  <  1:959543/140‑1 (MQ=255)
                     cAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGAt                                                                                                                  >  1:1212363/1‑140 (MQ=255)
                           aaTCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCAtt                                                                                                            >  1:2374454/1‑140 (MQ=255)
                           aaTCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCAtt                                                                                                            <  2:1205146/140‑1 (MQ=255)
                           aaTCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCAtt                                                                                                            >  1:22141/1‑140 (MQ=255)
                           aaTCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCAtt                                                                                                            >  1:1544778/1‑140 (MQ=255)
                             tCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTAt                                                                                                                      <  2:445164/128‑1 (MQ=255)
                             tCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTct                                                                                                          >  1:2087014/1‑140 (MQ=255)
                                  tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                     >  1:1689942/1‑140 (MQ=255)
                                  tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                     >  2:1466940/1‑140 (MQ=255)
                                  tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                     >  1:26051/1‑140 (MQ=255)
                                  tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                     >  1:932462/1‑140 (MQ=255)
                                  tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                     >  1:1060441/1‑140 (MQ=255)
                                  tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                     >  2:1860849/1‑140 (MQ=255)
                                  tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                     >  2:1916864/1‑140 (MQ=255)
                                      cTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaag                                                                                                 >  2:2192099/1‑140 (MQ=255)
                                      cTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaag                                                                                                 >  1:594998/1‑140 (MQ=255)
                                      cTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaag                                                                                                 >  1:594950/1‑140 (MQ=255)
                                       ttGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaagc                                                                                                >  2:323262/1‑140 (MQ=255)
                                       ttGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaagc                                                                                                >  1:1527111/1‑140 (MQ=255)
                                       ttGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaagc                                                                                                <  1:504630/140‑1 (MQ=255)
                                       ttGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTGTCTGTGTCTGGGAAGGCACGGGCCGCTATGGATTTCATTCTGCCTgcaagc                                                                                                >  1:1286644/1‑140 (MQ=255)
                                        tGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCTGTAATGGGGATACCGACAAAAATTGCGCCGTTTGTTTCTACGGGTCTGTTTTCCTTGTCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTgcaagca                                                                                               >  2:1112546/1‑140 (MQ=255)
                                        tGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCTGTAATGGGGATACCGACAAAAATTGCGCCGTTTGTTTCTACGGGTCTGTTTTCCTTGTCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTgcaagca                                                                                               >  2:1096180/1‑140 (MQ=255)
                                        tGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCTGTAATGGGGATACCGACAAAAATTGCGCCGTTTGTTTCTACGGGTCTGTTTTCCTTGTCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTgcaagca                                                                                               >  2:1004191/1‑140 (MQ=255)
                                        tGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCTGTAATGGGGATACCGACAAAAATTGCGCCGTTTGTTTCTACGGGTCTGTTTTCCTTGTCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTgcaagca                                                                                               >  2:878510/1‑140 (MQ=255)
                                        tGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCTGTAATGGGGATACCGACAAAAATTGCGCCGTTTGTTTCTACGGGTCTGTTTTCCTTGTCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTgcaagca                                                                                               >  2:1004858/1‑140 (MQ=255)
                                        tGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaagca                                                                                               >  1:257340/1‑140 (MQ=255)
                                         gTAAACCGCACCCTGCATCCAATGCCGACGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaagcaa                                                                                              >  1:432726/1‑140 (MQ=255)
                                                      tGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAgacgac                                                                                 <  1:1027344/140‑1 (MQ=255)
                                                      tGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAgacgac                                                                                 <  1:367092/140‑1 (MQ=255)
                                                              aTGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGGTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCAtt                                                                         >  1:562965/1‑140 (MQ=255)
                                                               tGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTGTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTg                                                                        >  2:1142292/1‑140 (MQ=255)
                                                                  cGACGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGga                                                                     >  2:2393147/1‑140 (MQ=255)
                                                                  cGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGga                                                                     >  2:160747/1‑140 (MQ=255)
                                                                  cGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGga                                                                     >  2:533904/1‑140 (MQ=255)
                                                                  cGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGga                                                                     >  2:771624/1‑140 (MQ=255)
                                                                       ggCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGCGAAGGCAAGGGCCGCTATGGATTTAATTATGCCTGAAAGCAATTCAAAACACGACAAGTCATTGGGAGACtt                                                                >  2:1370010/1‑140 (MQ=255)
                                                                       ggCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTATGGGAAGGCAAGGGCCGCTATGGATTTAATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAAtt                                                                >  2:2042600/1‑140 (MQ=255)
                                                                         cgcCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAAtttt                                                              >  1:700619/1‑140 (MQ=255)
                                                                         cgcCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAAtttt                                                              >  2:2104603/1‑140 (MQ=255)
                                                                           ccGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACTACCAGTCATTGGGAGAATTTTTc                                                            >  2:2155409/1‑140 (MQ=255)
                                                                           ccGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTAATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCAATGGGAGAATTTTTc                                                            >  2:585387/1‑140 (MQ=255)
                                                                           ccGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTGTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTc                                                            >  1:1227493/1‑140 (MQ=255)
                                                                           ccGTGATGGGGATACCGACAAAAATTGCGCCCTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTc                                                            >  2:725157/1‑140 (MQ=255)
                                                                            cGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTcc                                                           >  1:1510287/1‑140 (MQ=255)
                                                                            cGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTcc                                                           >  1:1094938/1‑140 (MQ=255)
                                                                                aTGGGGATACCGACAAAACTTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAg                                                       <  1:2364277/140‑1 (MQ=255)
                                                                                     gATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCgtg                                                  >  2:314080/1‑140 (MQ=255)
                                                                                        aCCGACAACAAGTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCg                                               <  2:2343075/140‑1 (MQ=255)
                                                                                        aCCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCg                                               <  2:902157/140‑1 (MQ=255)
                                                                                          cGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGga                                             >  2:1983039/1‑140 (MQ=255)
                                                                                          cGACAAAAATAGCTCCTATAGTTTCAACCAGTCTGTTTTCTGTTTATGGTAAGGCAATGGCGCCTATTCATTTAATTCTGCATGCAAGCAAGTCAAAAGACGAACAGTAATTGCGATAATATTTCAGCAGCAgtgtatga                                             >  2:1469948/1‑136 (MQ=255)
                                                                                              aaaaaTTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATg                                         >  2:2191518/1‑140 (MQ=255)
                                                                                                aaaTTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGaa                                       <  1:1146440/140‑1 (MQ=255)
                                                                                                aaaTTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGaa                                       <  1:1210536/140‑1 (MQ=255)
                                                                                                      cgcCTTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAATGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGAAGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                 <  1:1598237/140‑1 (MQ=255)
                                                                                                      cgcCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                 <  1:1892671/140‑1 (MQ=255)
                                                                                                      cgcCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                 <  1:1001897/140‑1 (MQ=255)
                                                                                                      cgcCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                 <  1:1598049/140‑1 (MQ=255)
                                                                                                      cgcCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                 <  2:1639893/140‑1 (MQ=255)
                                                                                                      cgcCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                 <  1:508295/140‑1 (MQ=255)
                                                                                                      cgcCGGTCGTTTCAACCGGTCTGTTTACTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                 <  1:1306992/140‑1 (MQ=255)
                                                                                                          gttcgttTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTgaga                             >  1:1904396/1‑140 (MQ=255)
                                                                                                                 tCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAAt                      <  1:2148167/140‑1 (MQ=255)
                                                                                                                     ccGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAg                  <  2:490189/140‑1 (MQ=255)
                                                                                                                     ccGGGCTGTATTCTGTGTCTGGGAACGCACCGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGATAATTTTTCCGCAGGCGGGTCGGAGATGAAGTGGTTGAGAATTTAATCGAg                  <  2:319337/140‑1 (MQ=255)
                                                                                                                        gTCTGTTTTCTGTGTCTGGGAAGGCAATGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACAAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGccc               >  1:2170403/1‑139 (MQ=255)
                                                                                                                        gTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGc                                                     >  1:1584369/1‑102 (MQ=255)
                                                                                                                        gTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCg               >  1:2316172/1‑140 (MQ=255)
                                                                                                                        gTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCg               >  1:2170141/1‑140 (MQ=255)
                                                                                                                        gTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCg               >  1:1182142/1‑140 (MQ=255)
                                                                                                                        gTCTCTTTTCTGTGTCTGTTAATTCAATGGCAACAATTGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCg               >  2:735745/1‑140 (MQ=255)
                                                                                                                          cTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCt             >  2:1679511/1‑140 (MQ=255)
                                                                                                                               ttCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTAt        <  1:635293/140‑1 (MQ=255)
                                                                                                                                 ccttgtCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTGCAAGCAAAACAAAGGATGATCAGTCATTGGGAGAATTCTTCCGCAGACGTGTCGGAGATGAAGTGGTCGAGAACTTAATCGAGCCGCTACTATCa      <  1:878510/137‑1 (MQ=255)
                                                                                                                                 ccttgtCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTGCAAGCAAAACAAAGGATGATCAGTCATTGGGAGAATTCTTCCGCAGACGTGTCGGAGATGAAGTGGTCGAGAACTTAATCGAGCCGCTACTATCa      <  1:1112546/137‑1 (MQ=255)
                                                                                                                                 ccttgtCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTGCAAGCAAAACAAAGGATGATCAGTCATTGGGAGAATTCTTCCGCAGACGTGTCGGAGATGAAGTGGTCGAGAACTTAATCGAGCCGCTACTATCa      <  1:1096181/137‑1 (MQ=255)
                                                                                                                                 ccttgtCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTGCAAGCAAAACAAAGGATGATCAGTCATTGGGAGAATTCTTCCGCAGACGTGTCGGAGATGAAGTGGTCGAGAACTTAATCGAGCCGCTACTATCa      <  1:1004191/137‑1 (MQ=255)
                                                                                                                                 cTGTGTCTGGGAAGGCAAGGGCGGCTATGGATGTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCa      <  2:1891680/140‑1 (MQ=255)
                                                                                                                                  tgtgTCTGGGAAGGCAAGGGCCGATATGGATTTCATTATGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAg     >  1:2314591/1‑140 (MQ=255)
                                                                                                                                     gtCTGGGAACGCAAGGGCCGCTAAGGATTTCATTCTGCCTGCAAGCAACTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCa  <  1:1286449/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
ATTTGCTTGTCAACAATGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCA  >  NZ_CP034943/1061127‑1061399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: