Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,061,272 A→G 7.0% A133A (GCA→GCG hemY → protoporphyrinogen oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,061,2720AG 7.0% 532.0 / 21.5 171A133A (GCA→GCGhemYprotoporphyrinogen oxidase
Reads supporting (aligned to +/- strand):  ref base A (101/58);  new base G (6/6);  total (107/64)
Fisher's exact test for biased strand distribution p-value = 3.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.20e-01

Only 100 of 175 total aligned reads displayed.

TTGTCAACAATGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTGGC  >  NZ_CP034943/1061133‑1061410
                                                                                                                                           |                                                                                                                                          
ttGTCAACAATGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCa                                                                                                                                            <  2:2252713/140‑1 (MQ=255)
 tGTCAACAATGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCaa                                                                                                                                           >  1:1110933/1‑140 (MQ=255)
  gTCAACAATGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAg                                                                                                                                          >  2:121345/1‑140 (MQ=255)
  gTCAACAATGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAg                                                                                                                                          <  1:827391/140‑1 (MQ=255)
  gTCAACAATGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAg                                                                                                                                          >  2:635293/1‑140 (MQ=255)
   tCAACAATGCAACAGGTCAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAgg                                                                                                                                         >  1:1812200/1‑140 (MQ=255)
         aTGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCt                                                                                                                                   <  1:2287569/140‑1 (MQ=255)
          tGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTa                                                                                                                                  >  2:1892671/1‑140 (MQ=255)
          tGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTa                                                                                                                                  >  2:1001897/1‑140 (MQ=255)
          tGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTa                                                                                                                                  >  2:1598049/1‑140 (MQ=255)
          tGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTGGGTTGCACCCGGGGTGTTTTGTGTGTGTGGGACGGCAAGGGCGGCTa                                                                                                                                  >  2:10975/1‑140 (MQ=255)
               cAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGAt                                                                                                                             <  1:959543/140‑1 (MQ=255)
               cAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGAt                                                                                                                             >  1:1639893/1‑140 (MQ=255)
                    cAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCGATGGATTTCAt                                                                                                                        >  1:1244168/1‑140 (MQ=255)
                     aaTCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCAtt                                                                                                                       >  1:22141/1‑140 (MQ=255)
                     aaTCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCAtt                                                                                                                       <  2:1205146/140‑1 (MQ=255)
                       tCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTAt                                                                                                                                 >  1:445164/1‑128 (MQ=255)
                       tCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTct                                                                                                                     >  1:2087014/1‑140 (MQ=255)
                            tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGACGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                                >  2:301882/1‑140 (MQ=255)
                            tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                                >  2:1860849/1‑140 (MQ=255)
                            tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                                >  1:1689942/1‑140 (MQ=255)
                            tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                                >  2:1466940/1‑140 (MQ=255)
                            tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                                >  2:1916864/1‑140 (MQ=255)
                            tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                                >  1:932462/1‑140 (MQ=255)
                            tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                                >  1:1060441/1‑140 (MQ=255)
                                cTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaag                                                                                                            >  1:594998/1‑140 (MQ=255)
                                 ttGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaagc                                                                                                           <  1:1593978/140‑1 (MQ=255)
                                 ttGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaagc                                                                                                           >  1:1527111/1‑140 (MQ=255)
                                 ttGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaagc                                                                                                           >  2:323262/1‑140 (MQ=255)
                                 ttGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTATGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGACAgcaagc                                                                                                           >  1:1538806/1‑140 (MQ=255)
                                  tGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCTGTAATGGGGATACCGACAAAAATTGCGCCGTTTGTTTCTACGGGTCTGTTTTCCTTGTCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTgcaagca                                                                                                          >  2:1004191/1‑140 (MQ=255)
                                  tGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCTGTAATGGGGATACCGACAAAAATTGCGCCGTTTGTTTCTACGGGTCTGTTTTCCTTGTCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTgcaagca                                                                                                          >  2:1096180/1‑140 (MQ=255)
                                  tGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCTGTAATGGGGATACCGACAAAAATTGCGCCGTTTGTTTCTACGGGTCTGTTTTCCTTGTCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTgcaagca                                                                                                          >  2:1112546/1‑140 (MQ=255)
                                  tGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCTGTAATGGGGATACCGACAAAAATTGCGCCGTTTGTTTCTACGGGTCTGTTTTCCTTGTCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTgcaagca                                                                                                          >  2:878510/1‑140 (MQ=255)
                                   gTAAACCGCACCCTGCATCCAATGCCGACGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaagcaa                                                                                                         >  1:432726/1‑140 (MQ=255)
                                   gTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCt                                                                                                                                   >  2:1454257/1‑114 (MQ=255)
                                   gTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCt                                                                                                                                   <  1:1454257/114‑1 (MQ=255)
                                   gTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaagcaa                                                                                                         >  1:1288798/1‑140 (MQ=255)
                                           cACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAg                                                                                                 >  1:490189/1‑140 (MQ=255)
                                           cACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAg                                                                                                 >  2:1146440/1‑140 (MQ=255)
                                                tGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACACAAAGTGCGCCGTTCGTTTCAACCGGTCTGTTTTCGGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAgacgac                                                                                            <  1:1114667/140‑1 (MQ=255)
                                                tGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAgacgac                                                                                            <  1:2407667/140‑1 (MQ=255)
                                                tGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAgacgac                                                                                            <  1:1027344/140‑1 (MQ=255)
                                                tGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGACTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAgacgac                                                                                            <  1:1161128/140‑1 (MQ=255)
                                                        aTGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCAtt                                                                                    >  1:259600/1‑140 (MQ=255)
                                                        aTGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGGTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCAtt                                                                                    >  1:562965/1‑140 (MQ=255)
                                                         tGCCGACGGGCGCCGGGAGGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTg                                                                                   >  2:533090/1‑140 (MQ=255)
                                                         tGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTg                                                                                   >  2:2196550/1‑140 (MQ=255)
                                                            cGACGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGga                                                                                >  2:2393147/1‑140 (MQ=255)
                                                            cGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGga                                                                                >  2:771624/1‑140 (MQ=255)
                                                            cGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGga                                                                                >  2:533904/1‑140 (MQ=255)
                                                            cGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGga                                                                                >  2:160747/1‑140 (MQ=255)
                                                                   cgcCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAAtttt                                                                         >  1:700619/1‑140 (MQ=255)
                                                                   cgcCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAAtttt                                                                         >  1:1848934/1‑140 (MQ=255)
                                                                   cgcCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAAtttt                                                                         >  2:2104603/1‑140 (MQ=255)
                                                                     ccGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACTACCAGTCATTGGGAGAATTTTTc                                                                       >  2:2155409/1‑140 (MQ=255)
                                                                     ccGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTc                                                                       >  1:636612/1‑140 (MQ=255)
                                                                     ccGTGATGGGGATACCGACAAAAATTGCGCCCTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTc                                                                       >  2:725157/1‑140 (MQ=255)
                                                                          aTGGGGATACCGACAAAACTTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAg                                                                  <  1:2364277/140‑1 (MQ=255)
                                                                                aTACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCgtgt                                                            <  1:1573014/140‑1 (MQ=255)
                                                                                aTACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCgtgt                                                            <  1:58430/140‑1 (MQ=255)
                                                                                aTACCGACAAAAAGTGCGCCGTTCGTTACAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCgtgt                                                            <  1:620304/140‑1 (MQ=255)
                                                                                  aCCGACAACAAGTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCg                                                          <  2:2343075/140‑1 (MQ=255)
                                                                                  aCCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCg                                                          >  2:2068980/1‑140 (MQ=255)
                                                                                  aCCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCg                                                          <  2:902157/140‑1 (MQ=255)
                                                                                  aCCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCg                                                          >  2:1042226/1‑140 (MQ=255)
                                                                                    cGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGga                                                        >  2:1983039/1‑140 (MQ=255)
                                                                                    cGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCAGCAGGCGTGTCGga                                                        >  2:423091/1‑140 (MQ=255)
                                                                                        aaaaaTTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATg                                                    >  2:2191518/1‑140 (MQ=255)
                                                                                          aaaTTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGaa                                                  <  1:1210536/140‑1 (MQ=255)
                                                                                                cgcCTTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAATGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGAAGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                            <  1:1598237/140‑1 (MQ=255)
                                                                                                cgcCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                            <  2:1639893/140‑1 (MQ=255)
                                                                                                cgcCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                            <  1:508295/140‑1 (MQ=255)
                                                                                                cgcCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                            <  1:1001897/140‑1 (MQ=255)
                                                                                                    gttcgttTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTgaga                                        >  1:1904396/1‑140 (MQ=255)
                                                                                                     ttcgttTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGaa                                       >  1:1607257/1‑140 (MQ=255)
                                                                                                           tCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAAt                                 <  2:25111/140‑1 (MQ=255)
                                                                                                           tCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAAt                                 <  1:2148167/140‑1 (MQ=255)
                                                                                                               ccGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAg                             <  2:132439/140‑1 (MQ=255)
                                                                                                               ccGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAg                             <  2:490189/140‑1 (MQ=255)
                                                                                                               ccGGGCTGTATTCTGTGTCTGGGAACGCACCGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGATAATTTTTCCGCAGGCGGGTCGGAGATGAAGTGGTTGAGAATTTAATCGAg                             <  2:319337/140‑1 (MQ=255)
                                                                                                                  gTCTGTTTTCTGTGTCTGGGAAGGCAATGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACAAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGccc                          >  1:2170403/1‑139 (MQ=255)
                                                                                                                  gTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCg                          >  1:2316172/1‑140 (MQ=255)
                                                                                                                  gTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCg                          >  1:1182142/1‑140 (MQ=255)
                                                                                                                  gTCTCTTTTCTGTGTCTGTTAATTCAATGGCAACAATTGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCg                          >  2:735745/1‑140 (MQ=255)
                                                                                                                    cTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCt                        >  2:1679511/1‑140 (MQ=255)
                                                                                                                         ttCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTAt                   <  1:121345/140‑1 (MQ=255)
                                                                                                                           ccttgtCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTGCAAGCAAAACAAAGGATGATCAGTCATTGGGAGAATTCTTCCGCAGACGTGTCGGAGATGAAGTGGTCGAGAACTTAATCGAGCCGCTACTATCa                 <  1:1004191/137‑1 (MQ=255)
                                                                                                                           ccttgtCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTGCAAGCAAAACAAAGGATGATCAGTCATTGGGAGAATTCTTCCGCAGACGTGTCGGAGATGAAGTGGTCGAGAACTTAATCGAGCCGCTACTATCa                 <  1:878510/137‑1 (MQ=255)
                                                                                                                           ccttgtCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTGCAAGCAAAACAAAGGATGATCAGTCATTGGGAGAATTCTTCCGCAGACGTGTCGGAGATGAAGTGGTCGAGAACTTAATCGAGCCGCTACTATCa                 <  1:1096181/137‑1 (MQ=255)
                                                                                                                           ccttgtCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTGCAAGCAAAACAAAGGATGATCAGTCATTGGGAGAATTCTTCCGCAGACGTGTCGGAGATGAAGTGGTCGAGAACTTAATCGAGCCGCTACTATCa                 <  1:1096180/137‑1 (MQ=255)
                                                                                                                           cTGTGTCTGGGAAGGCAAGGGCGGCTATGGATGTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCa                 <  2:1891680/140‑1 (MQ=255)
                                                                                                                           cTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCa                 <  1:2149283/140‑1 (MQ=255)
                                                                                                                            tgtgTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAg                >  1:301343/1‑140 (MQ=255)
                                                                                                                            tgtgTCTGGGAAGGCAAGGGCCGATATGGATTTCATTATGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAg                >  1:2314591/1‑140 (MQ=255)
                                                                                                                               gtCTGGGAACGCAAGGGCCGCTAAGGATTTCATTCTGCCTGCAAGCAACTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCa             <  1:1286449/140‑1 (MQ=255)
                                                                                                                                    ggAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGc                            <  1:2390896/120‑1 (MQ=255)
                                                                                                                                          cAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTGGc  >  2:1512094/1‑140 (MQ=255)
                                                                                                                                          cAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTGGc  >  2:453999/1‑140 (MQ=255)
                                                                                                                                          cAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATAGAGCCGCTTCTATCAGGCATCTACGCTGGc  >  2:2301531/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TTGTCAACAATGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTGGC  >  NZ_CP034943/1061133‑1061410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: