Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,061,275 G→A 7.2% R134R (AGG→AGA hemY → protoporphyrinogen oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,061,2750GA 7.2% 525.0 / 20.6 167R134R (AGG→AGAhemYprotoporphyrinogen oxidase
Reads supporting (aligned to +/- strand):  ref base G (98/57);  new base A (6/6);  total (104/63)
Fisher's exact test for biased strand distribution p-value = 3.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 172 total aligned reads displayed.

TCAACAATGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTGGCGACA  >  NZ_CP034943/1061136‑1061414
                                                                                                                                           |                                                                                                                                           
tCAACAATGCAACAGGTCAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAgg                                                                                                                                             >  1:1812200/1‑140 (MQ=255)
tCAACAATGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAgg                                                                                                                                             >  1:1205146/1‑140 (MQ=255)
      aTGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCt                                                                                                                                       <  1:462377/140‑1 (MQ=255)
      aTGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCt                                                                                                                                       <  1:2287569/140‑1 (MQ=255)
       tGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGACGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCGGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCtc                                                                                                                                      >  2:1306992/1‑139 (MQ=255)
       tGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTa                                                                                                                                      >  2:1001897/1‑140 (MQ=255)
       tGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTGGGTTGCACCCGGGGTGTTTTGTGTGTGTGGGACGGCAAGGGCGGCTa                                                                                                                                      >  2:10975/1‑140 (MQ=255)
       tGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATAAAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTa                                                                                                                                      >  2:1598237/1‑140 (MQ=255)
        gCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTAt                                                                                                                                     <  2:280318/140‑1 (MQ=255)
            cAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGAt                                                                                                                                 >  1:1639893/1‑140 (MQ=255)
            cAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGAt                                                                                                                                 >  1:1212363/1‑140 (MQ=255)
                 cAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCGATGGATTTCAt                                                                                                                            >  1:1244168/1‑140 (MQ=255)
                  aaTCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCAtt                                                                                                                           >  1:22141/1‑140 (MQ=255)
                  aaTCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCAtt                                                                                                                           <  2:1812200/140‑1 (MQ=255)
                  aaTCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCAtt                                                                                                                           <  2:1205146/140‑1 (MQ=255)
                  aaTCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCAtt                                                                                                                           >  1:1544778/1‑140 (MQ=255)
                    tCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTAt                                                                                                                                     <  2:445164/128‑1 (MQ=255)
                    tCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTct                                                                                                                         >  1:2087014/1‑140 (MQ=255)
                         tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                                    >  1:26051/1‑140 (MQ=255)
                         tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                                    >  1:961574/1‑140 (MQ=255)
                         tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                                    >  1:932462/1‑140 (MQ=255)
                         tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                                    >  2:1466940/1‑140 (MQ=255)
                         tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                                    >  2:2161382/1‑140 (MQ=255)
                         tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                                    >  1:1060441/1‑140 (MQ=255)
                             cTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaag                                                                                                                >  1:594998/1‑140 (MQ=255)
                             cTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaag                                                                                                                >  1:594950/1‑140 (MQ=255)
                              ttGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaagc                                                                                                               <  1:1593978/140‑1 (MQ=255)
                              ttGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaagc                                                                                                               >  1:1527111/1‑140 (MQ=255)
                              ttGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTGTCTGTGTCTGGGAAGGCACGGGCCGCTATGGATTTCATTCTGCCTgcaagc                                                                                                               >  1:1286644/1‑140 (MQ=255)
                               tGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCTGTAATGGGGATACCGACAAAAATTGCGCCGTTTGTTTCTACGGGTCTGTTTTCCTTGTCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTgcaagca                                                                                                              >  2:878510/1‑140 (MQ=255)
                               tGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCTGTAATGGGGATACCGACAAAAATTGCGCCGTTTGTTTCTACGGGTCTGTTTTCCTTGTCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTgcaagca                                                                                                              >  2:1004191/1‑140 (MQ=255)
                               tGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCTGTAATGGGGATACCGACAAAAATTGCGCCGTTTGTTTCTACGGGTCTGTTTTCCTTGTCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTgcaagca                                                                                                              >  2:1096181/1‑140 (MQ=255)
                               tGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCTGTAATGGGGATACCGACAAAAATTGCGCCGTTTGTTTCTACGGGTCTGTTTTCCTTGTCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTgcaagca                                                                                                              >  2:1112546/1‑140 (MQ=255)
                               tGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaagca                                                                                                              >  1:257340/1‑140 (MQ=255)
                                gTAAACCGCACCCTGCATCCAATGCCGACGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaagcaa                                                                                                             >  1:432726/1‑140 (MQ=255)
                                gTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCt                                                                                                                                       <  1:1454257/114‑1 (MQ=255)
                                        cACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAg                                                                                                     >  1:490189/1‑140 (MQ=255)
                                        cACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAg                                                                                                     >  2:1146440/1‑140 (MQ=255)
                                             tGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAgacgac                                                                                                <  1:1027344/140‑1 (MQ=255)
                                             tGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGACTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAgacgac                                                                                                <  1:1161128/140‑1 (MQ=255)
                                                     aTGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGGTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCAtt                                                                                        >  1:562965/1‑140 (MQ=255)
                                                      tGCCGACGGGCGCCGGGAGGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTg                                                                                       >  2:533090/1‑140 (MQ=255)
                                                      tGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTg                                                                                       >  2:2196550/1‑140 (MQ=255)
                                                         cGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGga                                                                                    >  2:771624/1‑140 (MQ=255)
                                                         cGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGga                                                                                    >  2:160747/1‑140 (MQ=255)
                                                         cGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGga                                                                                    >  2:533904/1‑140 (MQ=255)
                                                              ggCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGCGAAGGCAAGGGCCGCTATGGATTTAATTATGCCTGAAAGCAATTCAAAACACGACAAGTCATTGGGAGACtt                                                                               >  2:1370010/1‑140 (MQ=255)
                                                              ggCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTATGGGAAGGCAAGGGCCGCTATGGATTTAATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAAtt                                                                               >  2:2042600/1‑140 (MQ=255)
                                                                cgcCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAAtttt                                                                             >  2:2104603/1‑140 (MQ=255)
                                                                  ccGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACTACCAGTCATTGGGAGAATTTTTc                                                                           >  2:2155409/1‑140 (MQ=255)
                                                                  ccGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTc                                                                           >  1:636612/1‑140 (MQ=255)
                                                                   cGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTcc                                                                          >  1:1094938/1‑140 (MQ=255)
                                                                   cGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTcc                                                                          <  2:730422/140‑1 (MQ=255)
                                                                       aTGGGGATACCGACAAAACTTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAg                                                                      <  1:2364277/140‑1 (MQ=255)
                                                                            gATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCgtg                                                                 >  2:314080/1‑140 (MQ=255)
                                                                             aTACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCgtgt                                                                <  1:1573014/140‑1 (MQ=255)
                                                                             aTACCGACAAAAAGTGCGCCGTTCGTTACAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCgtgt                                                                <  1:620304/140‑1 (MQ=255)
                                                                               aCCGACAACAAGTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCg                                                              <  2:2343075/140‑1 (MQ=255)
                                                                               aCCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCg                                                              >  2:1042226/1‑140 (MQ=255)
                                                                               aCCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCg                                                              <  2:902157/140‑1 (MQ=255)
                                                                                 cGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCAGCAGGCGTGTCGga                                                            >  2:423091/1‑140 (MQ=255)
                                                                                 cGACAAAAATAGCTCCTATAGTTTCAACCAGTCTGTTTTCTGTTTATGGTAAGGCAATGGCGCCTATTCATTTAATTCTGCATGCAAGCAAGTCAAAAGACGAACAGTAATTGCGATAATATTTCAGCAGCAgtgtatga                                                            >  2:1469948/1‑136 (MQ=255)
                                                                                     aaaaaTTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATg                                                        >  2:2191518/1‑140 (MQ=255)
                                                                                       aaaTTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGaa                                                      <  1:1146440/140‑1 (MQ=255)
                                                                                             cgcCTTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAATGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGAAGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                                <  1:1598237/140‑1 (MQ=255)
                                                                                             cgcCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                                <  1:1001897/140‑1 (MQ=255)
                                                                                             cgcCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                                <  1:1892671/140‑1 (MQ=255)
                                                                                             cgcCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                                <  1:508295/140‑1 (MQ=255)
                                                                                             cgcCGGTCGTTTCAACCGGTCTGTTTACTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                                <  1:1306992/140‑1 (MQ=255)
                                                                                                 gttcgttTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTgaga                                            >  1:1904396/1‑140 (MQ=255)
                                                                                                        tCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAAt                                     <  2:25111/140‑1 (MQ=255)
                                                                                                        tCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAAt                                     <  1:2148167/140‑1 (MQ=255)
                                                                                                            ccGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAg                                 <  2:1651755/140‑1 (MQ=255)
                                                                                                            ccGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAg                                 <  2:490189/140‑1 (MQ=255)
                                                                                                            ccGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAg                                 <  2:319352/140‑1 (MQ=255)
                                                                                                            ccGGTCTGTTTTCGGTGTCGGGGAAGGCACGGGCCGCTATGGATTTCATTCTGCCTGCACGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGAGGAAGTGGTTGAGAATTTAATCGAg                                 <  2:1880338/140‑1 (MQ=255)
                                                                                                               gTCTGTTTTCTGTGTCTGGGAAGGCAATGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACAAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGccc                              >  1:2170403/1‑139 (MQ=255)
                                                                                                               gTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGc                                                                    <  2:1584369/102‑1 (MQ=255)
                                                                                                               gTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCg                              >  1:829524/1‑140 (MQ=255)
                                                                                                               gTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCg                              >  1:2316172/1‑140 (MQ=255)
                                                                                                               gTCTCTTTTCTGTGTCTGTTAATTCAATGGCAACAATTGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCg                              >  2:735745/1‑140 (MQ=255)
                                                                                                                tCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGc                             >  2:1945099/1‑140 (MQ=255)
                                                                                                                tCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGc                             >  2:649961/1‑140 (MQ=255)
                                                                                                                      ttCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTAt                       <  1:121345/140‑1 (MQ=255)
                                                                                                                        ccttgtCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTGCAAGCAAAACAAAGGATGATCAGTCATTGGGAGAATTCTTCCGCAGACGTGTCGGAGATGAAGTGGTCGAGAACTTAATCGAGCCGCTACTATCa                     <  1:1004191/137‑1 (MQ=255)
                                                                                                                        ccttgtCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTGCAAGCAAAACAAAGGATGATCAGTCATTGGGAGAATTCTTCCGCAGACGTGTCGGAGATGAAGTGGTCGAGAACTTAATCGAGCCGCTACTATCa                     <  1:878510/137‑1 (MQ=255)
                                                                                                                        ccttgtCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTGCAAGCAAAACAAAGGATGATCAGTCATTGGGAGAATTCTTCCGCAGACGTGTCGGAGATGAAGTGGTCGAGAACTTAATCGAGCCGCTACTATCa                     <  1:1096181/137‑1 (MQ=255)
                                                                                                                        ccttgtCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTGCAAGCAAAACAAAGGATGATCAGTCATTGGGAGAATTCTTCCGCAGACGTGTCGGAGATGAAGTGGTCGAGAACTTAATCGAGCCGCTACTATCa                     <  1:1112546/137‑1 (MQ=255)
                                                                                                                        cTGTGTCTGGGAAGGCAAGGGCGGCTATGGATGTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCa                     <  2:1891680/140‑1 (MQ=255)
                                                                                                                        cTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCa                     <  1:2149283/140‑1 (MQ=255)
                                                                                                                        cTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCa                     <  2:191059/140‑1 (MQ=255)
                                                                                                                         tgtgTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAg                    >  1:301343/1‑140 (MQ=255)
                                                                                                                         tgtgTCTGGGAAGGCAAGGGCCGATATGGATTTCATTATGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAg                    >  1:2314591/1‑140 (MQ=255)
                                                                                                                                 ggAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGc                                <  1:2390896/120‑1 (MQ=255)
                                                                                                                                 ggAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGc                                >  2:2390896/1‑120 (MQ=255)
                                                                                                                                  gAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACg           <  1:1679511/140‑1 (MQ=255)
                                                                                                                                       cAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATAGAGCCGCTTCTATCAGGCATCTACGCTGGc      >  2:2301531/1‑140 (MQ=255)
                                                                                                                                         aGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                                >  1:2369011/1‑96 (MQ=255)
                                                                                                                                         aGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTGGCGa    >  1:1743431/1‑140 (MQ=255)
                                                                                                                                           ggCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTCGCGACa  >  2:2060769/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TCAACAATGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTGGCGACA  >  NZ_CP034943/1061136‑1061414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: