Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,061,278 C→T 7.4% A135A (GCC→GCT hemY → protoporphyrinogen oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,061,2780CT 7.4% 526.4 / 20.6 170A135A (GCC→GCThemYprotoporphyrinogen oxidase
Reads supporting (aligned to +/- strand):  ref base C (98/56);  new base T (6/6);  total (107/63)
Fisher's exact test for biased strand distribution p-value = 3.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 170 total aligned reads displayed.

ATGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTGGCGACA  >  NZ_CP034943/1061142‑1061414
                                                                                                                                        |                                                                                                                                        
 tGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTa                                                                                                                                      >  2:1892671/1‑140 (MQ=255)
 tGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTa                                                                                                                                      >  2:1001897/1‑140 (MQ=255)
 tGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTa                                                                                                                                      >  2:1598049/1‑140 (MQ=255)
 tGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATAAAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTa                                                                                                                                      >  2:1598237/1‑140 (MQ=255)
      cAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGAt                                                                                                                                 <  1:2122980/140‑1 (MQ=255)
      cAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGAt                                                                                                                                 <  1:959543/140‑1 (MQ=255)
      cAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGAt                                                                                                                                 >  1:1639893/1‑140 (MQ=255)
      cAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGAt                                                                                                                                 <  1:558917/140‑1 (MQ=255)
            aaTCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCAtt                                                                                                                           <  2:1812200/140‑1 (MQ=255)
            aaTCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCAtt                                                                                                                           >  1:22141/1‑140 (MQ=255)
            aaTCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCAtt                                                                                                                           >  1:1544778/1‑140 (MQ=255)
            aaTCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCAtt                                                                                                                           <  2:1205146/140‑1 (MQ=255)
              tCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTAt                                                                                                                                     >  1:445164/1‑128 (MQ=255)
              tCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTct                                                                                                                         >  1:2087014/1‑140 (MQ=255)
                   tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGACGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                                    >  2:301882/1‑140 (MQ=255)
                   tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                                    >  2:1466940/1‑140 (MQ=255)
                   tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                                    >  2:1916864/1‑140 (MQ=255)
                   tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                                    >  1:902157/1‑140 (MQ=255)
                   tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                                    >  1:1689942/1‑140 (MQ=255)
                   tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                                    >  1:1060441/1‑140 (MQ=255)
                   tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                                    >  2:100396/1‑140 (MQ=255)
                       cTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaag                                                                                                                >  2:2192099/1‑140 (MQ=255)
                       cTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaag                                                                                                                >  1:594998/1‑140 (MQ=255)
                        ttGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTATGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGACAgcaagc                                                                                                               >  1:1538806/1‑140 (MQ=255)
                         tGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCTGTAATGGGGATACCGACAAAAATTGCGCCGTTTGTTTCTACGGGTCTGTTTTCCTTGTCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTgcaagca                                                                                                              >  2:1096181/1‑140 (MQ=255)
                         tGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCTGTAATGGGGATACCGACAAAAATTGCGCCGTTTGTTTCTACGGGTCTGTTTTCCTTGTCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTgcaagca                                                                                                              >  2:1004858/1‑140 (MQ=255)
                         tGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCTGTAATGGGGATACCGACAAAAATTGCGCCGTTTGTTTCTACGGGTCTGTTTTCCTTGTCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTgcaagca                                                                                                              >  2:1096180/1‑140 (MQ=255)
                         tGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCTGTAATGGGGATACCGACAAAAATTGCGCCGTTTGTTTCTACGGGTCTGTTTTCCTTGTCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTgcaagca                                                                                                              >  2:878510/1‑140 (MQ=255)
                          gTAAACCGCACCCTGCATCCAATGCCGACGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaagcaa                                                                                                             >  1:432726/1‑140 (MQ=255)
                          gTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCt                                                                                                                                       >  2:1454257/1‑114 (MQ=255)
                          gTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaagcaa                                                                                                             >  1:1288798/1‑140 (MQ=255)
                                  cACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAg                                                                                                     >  1:490189/1‑140 (MQ=255)
                                  cACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAg                                                                                                     >  2:1210536/1‑140 (MQ=255)
                                       tGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACACAAAGTGCGCCGTTCGTTTCAACCGGTCTGTTTTCGGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAgacgac                                                                                                <  1:1114667/140‑1 (MQ=255)
                                       tGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAgacgac                                                                                                <  1:1027344/140‑1 (MQ=255)
                                       tGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAgacgac                                                                                                <  1:2407667/140‑1 (MQ=255)
                                       tGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGACTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAgacgac                                                                                                <  1:1161128/140‑1 (MQ=255)
                                               aTGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCAtt                                                                                        >  1:259600/1‑140 (MQ=255)
                                                tGCCGACGGGCGCCGGGAGGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTg                                                                                       >  2:533090/1‑140 (MQ=255)
                                                tGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTg                                                                                       >  2:2196550/1‑140 (MQ=255)
                                                tGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTGTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTg                                                                                       >  2:1142292/1‑140 (MQ=255)
                                                   cGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGga                                                                                    >  2:771624/1‑140 (MQ=255)
                                                        ggCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTATGGGAAGGCAAGGGCCGCTATGGATTTAATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAAtt                                                                               >  2:2042600/1‑140 (MQ=255)
                                                          cgcCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAAtttt                                                                             >  2:2104603/1‑140 (MQ=255)
                                                          cgcCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAAtttt                                                                             >  1:700619/1‑140 (MQ=255)
                                                          cgcCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAAtttt                                                                             >  1:1848934/1‑140 (MQ=255)
                                                            ccGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACTACCAGTCATTGGGAGAATTTTTc                                                                           >  2:2155409/1‑140 (MQ=255)
                                                            ccGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTAATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCAATGGGAGAATTTTTc                                                                           >  2:585387/1‑140 (MQ=255)
                                                            ccGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTGTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTc                                                                           >  1:1227493/1‑140 (MQ=255)
                                                             cGTGATGGGGATACCGACAACAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTcc                                                                          <  2:1110933/140‑1 (MQ=255)
                                                             cGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTcc                                                                          >  1:1094938/1‑140 (MQ=255)
                                                             cGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTcc                                                                          <  2:730422/140‑1 (MQ=255)
                                                             cGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTcc                                                                          >  1:1510287/1‑140 (MQ=255)
                                                                      gATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCgtg                                                                 >  2:314080/1‑140 (MQ=255)
                                                                       aTACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCgtgt                                                                <  1:58430/140‑1 (MQ=255)
                                                                         aCCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCg                                                              >  2:2068980/1‑140 (MQ=255)
                                                                         aCCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCg                                                              <  2:902157/140‑1 (MQ=255)
                                                                         aCCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCg                                                              >  1:2215770/1‑140 (MQ=255)
                                                                           cGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCAGCAGGCGTGTCGga                                                            >  2:423091/1‑140 (MQ=255)
                                                                                 aaaTTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGaa                                                      <  1:1210536/140‑1 (MQ=255)
                                                                                       cgcCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                                <  1:508295/140‑1 (MQ=255)
                                                                                       cgcCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                                <  1:1598049/140‑1 (MQ=255)
                                                                                       cgcCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                                <  2:1639893/140‑1 (MQ=255)
                                                                                       cgcCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                                <  1:1001897/140‑1 (MQ=255)
                                                                                       cgcCGGTCGTTTCAACCGGTCTGTTTACTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                                <  1:1306992/140‑1 (MQ=255)
                                                                                           gttcgttTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTgaga                                            >  1:1904396/1‑140 (MQ=255)
                                                                                            ttcgttTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGaa                                           >  1:1607257/1‑140 (MQ=255)
                                                                                                  tCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAAt                                     <  2:25111/140‑1 (MQ=255)
                                                                                                      ccGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAg                                 <  2:319352/140‑1 (MQ=255)
                                                                                                      ccGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAg                                 <  2:132439/140‑1 (MQ=255)
                                                                                                      ccGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAg                                 <  2:490189/140‑1 (MQ=255)
                                                                                                      ccGGTCTGTTTTCGGTGTCGGGGAAGGCACGGGCCGCTATGGATTTCATTCTGCCTGCACGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGAGGAAGTGGTTGAGAATTTAATCGAg                                 <  2:1880338/140‑1 (MQ=255)
                                                                                                         gTCTGTTTTCTGTGTCTGGGAAGGCAATGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACAAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGccc                              >  1:2170403/1‑139 (MQ=255)
                                                                                                         gTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGc                                                                    >  1:1584369/1‑102 (MQ=255)
                                                                                                         gTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCg                              >  1:2316172/1‑140 (MQ=255)
                                                                                                         gTCTCTTTTCTGTGTCTGTTAATTCAATGGCAACAATTGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCg                              >  2:735745/1‑140 (MQ=255)
                                                                                                          tCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGc                             >  2:649961/1‑140 (MQ=255)
                                                                                                          tCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGc                             >  2:1945099/1‑140 (MQ=255)
                                                                                                           cTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCt                            >  2:1679511/1‑140 (MQ=255)
                                                                                                                ttCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTAt                       <  1:121345/140‑1 (MQ=255)
                                                                                                                ttCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTAt                       <  1:2317881/140‑1 (MQ=255)
                                                                                                                ttCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTAt                       <  1:635293/140‑1 (MQ=255)
                                                                                                                  ccttgtCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTGCAAGCAAAACAAAGGATGATCAGTCATTGGGAGAATTCTTCCGCAGACGTGTCGGAGATGAAGTGGTCGAGAACTTAATCGAGCCGCTACTATCa                     <  1:878510/137‑1 (MQ=255)
                                                                                                                  ccttgtCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTGCAAGCAAAACAAAGGATGATCAGTCATTGGGAGAATTCTTCCGCAGACGTGTCGGAGATGAAGTGGTCGAGAACTTAATCGAGCCGCTACTATCa                     <  1:1004191/137‑1 (MQ=255)
                                                                                                                  ccttgtCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTGCAAGCAAAACAAAGGATGATCAGTCATTGGGAGAATTCTTCCGCAGACGTGTCGGAGATGAAGTGGTCGAGAACTTAATCGAGCCGCTACTATCa                     <  1:1096181/137‑1 (MQ=255)
                                                                                                                  ccttgtCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTGCAAGCAAAACAAAGGATGATCAGTCATTGGGAGAATTCTTCCGCAGACGTGTCGGAGATGAAGTGGTCGAGAACTTAATCGAGCCGCTACTATCa                     <  1:1112546/137‑1 (MQ=255)
                                                                                                                  cTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCa                     <  1:2149283/140‑1 (MQ=255)
                                                                                                                   tgtgTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAg                    >  1:1763990/1‑140 (MQ=255)
                                                                                                                   tgtgTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAg                    >  1:301343/1‑140 (MQ=255)
                                                                                                                   tgtgTCTGGGAAGGCAAGGGCCGATATGGATTTCATTATGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAg                    >  1:2314591/1‑140 (MQ=255)
                                                                                                                      gtCTGGGAACGCAAGGGCCGCTAAGGATTTCATTCTGCCTGCAAGCAACTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCa                 <  1:1286449/140‑1 (MQ=255)
                                                                                                                           ggAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGc                                <  1:2390896/120‑1 (MQ=255)
                                                                                                                           ggAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGc                                >  2:2390896/1‑120 (MQ=255)
                                                                                                                               ggCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTg        >  1:59736/1‑140 (MQ=255)
                                                                                                                                 cAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTGGc      >  2:453999/1‑140 (MQ=255)
                                                                                                                                 cAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTGGc      >  2:1512094/1‑140 (MQ=255)
                                                                                                                                 cAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATAGAGCCGCTTCTATCAGGCATCTACGCTGGc      >  2:2301531/1‑140 (MQ=255)
                                                                                                                                   aGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                                >  1:2369011/1‑96 (MQ=255)
                                                                                                                                   aGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                                <  2:2369011/96‑1 (MQ=255)
                                                                                                                                     ggCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCATTCATTGGGAGAATTTTTCCGCAGCCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGATGGCGACa  >  2:2188916/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                        
ATGCAACAGGACAATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTGGCGACA  >  NZ_CP034943/1061142‑1061414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: