Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 344,548 T→C 5.2% Y132Y (TAT→TAC putR → proline utilization transcriptional regulator PutR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943344,5480TC 5.2% 432.3 / 7.4 133Y132Y (TAT→TACputRproline utilization transcriptional regulator PutR
Reads supporting (aligned to +/- strand):  ref base T (78/48);  new base C (4/3);  total (82/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

TTCGCGTCAATAAAATTGAAAGCATTGGGTTAAACCAGAGGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAA  >  NZ_CP034943/344409‑344683
                                                                                                                                           |                                                                                                                                       
ttCGCGTCAATAAAATTGAAAGCATTGGGTTAAACCAGAGGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTAt                                                                                                                                         >  2:1244396/1‑140 (MQ=255)
     gTCAATAAAATTGAAAGCATTGGGTTAAACCAGAGGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGAc                                                                                                                                    <  2:2347193/140‑1 (MQ=255)
      tCAATAAAATTGAAAGCATTGGGTTAAACCAGAGGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGAcc                                                                                                                                   <  1:2053651/140‑1 (MQ=255)
      tCAATAAAATTGAAAGCATTGGGTTAAACCAGAGGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGAcc                                                                                                                                   <  1:1267113/140‑1 (MQ=255)
        aaTAAAATTGAAAGCATTGGGTTAAACCAGAGGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACctc                                                                                                                                 >  1:1575945/1‑140 (MQ=255)
            aaaTTGAAAGCATTGGGTTAAACCAGAGGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCtttt                                                                                                                             <  1:1056744/140‑1 (MQ=255)
            aaaTTGAAAGCATTGGGTTAAACCAGAGGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCtttt                                                                                                                             <  1:226250/140‑1 (MQ=255)
            aaaTTGAAAGCATTGGGTTAAACCAGAGGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCtttt                                                                                                                             <  1:2260511/140‑1 (MQ=255)
            aaaTTGAAAGCATTGGGTTAAACCAGAGGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCtttt                                                                                                                             <  1:1129676/140‑1 (MQ=255)
                        ttGGGTTAAACCAGAGGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGaaa                                                                                                                 <  1:873882/140‑1 (MQ=255)
                         tGGGTTAAACCAGAGGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGaaaa                                                                                                                >  2:1417074/1‑140 (MQ=255)
                                aaCCAGAGGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTta                                                                                                         >  1:716562/1‑140 (MQ=255)
                                aaCCAGAGGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTta                                                                                                         >  1:1086800/1‑140 (MQ=255)
                                aaCCAGAGGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAAGCATTta                                                                                                         >  1:1019004/1‑140 (MQ=255)
                                aaCCAGAGGGTGGTAGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTta                                                                                                         >  1:1175369/1‑140 (MQ=255)
                                     gagGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTAt                                                                                                                                         <  2:1271196/103‑1 (MQ=255)
                                     gagGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTAt                                                                                                                                         >  1:1664670/1‑103 (MQ=255)
                                     gagGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATaaaa                                                                                                    >  2:1572318/1‑140 (MQ=255)
                                     gagGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATaaaa                                                                                                    >  2:257015/1‑140 (MQ=255)
                                     gagGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATaaaa                                                                                                    >  2:1383945/1‑140 (MQ=255)
                                          tggtCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAAc                                                                                               >  2:205379/1‑140 (MQ=255)
                                          tggtCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGGCGGGAAAATCATTTATAAAACGAAc                                                                                               >  2:715500/1‑140 (MQ=255)
                                           ggtCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGAATTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACa                                                                                              >  2:303136/1‑140 (MQ=255)
                                           ggtCGGGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGACGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCAGTTAGAAAACGAACa                                                                                              >  2:1805193/1‑140 (MQ=255)
                                            gtCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACaa                                                                                             >  2:599032/1‑140 (MQ=255)
                                            gtCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACaa                                                                                             >  1:1457787/1‑140 (MQ=255)
                                            gtCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACaa                                                                                             >  1:5990/1‑140 (MQ=255)
                                            gtCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACaa                                                                                             >  2:2268142/1‑140 (MQ=255)
                                            gtCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACaa                                                                                             >  1:405301/1‑140 (MQ=255)
                                             tCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAg                                                                                            >  1:2361675/1‑140 (MQ=255)
                                             tCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAg                                                                                            >  2:320770/1‑140 (MQ=255)
                                                   gTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAg                                                                                      <  1:91775/140‑1 (MQ=255)
                                                                aGGGGAAGTCATGGGCTACATCTGGATTCAGGAGCTGGACCAAAATCTGACGGATGAAGAGCTCGACTTTTTGTACGAGACCTCCTTCCATGTCGGGAAAATCATTTATAAAACGAATAAATTGAAACAGGAAAAGgaag                                                                         >  1:1278619/1‑140 (MQ=255)
                                                                aGGGGAAGTCATGGGCTACATCTGGATTCAGGAGCTGGACCAAAATCTGACGGATGAAGAGCTCGACTTTTTGTACGAGACCTCCTTCCATGTCGGGAAAATCATTTATAAAACGAATAAATTGAAACAGGAAAAGgaag                                                                         >  1:1278980/1‑140 (MQ=255)
                                                                aGGGGAAGTCATGGGCTACATCTGGATTCAGGAGCTGGACCAAAATCTGACGGATGAAGAGCTCGACTTTTTGTACGAGACCTCCTTCCATGTCGGGAAAATCATTTATAAAACGAATAAATTGAAACAGGAAAAGgaag                                                                         >  1:512300/1‑140 (MQ=255)
                                                                 ggggAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATTTGGGTAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGgaaga                                                                        <  1:332783/140‑1 (MQ=255)
                                                                     aaGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaaaa                                                                    >  1:2160852/1‑140 (MQ=255)
                                                                     aaGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaaaa                                                                    >  1:1352220/1‑140 (MQ=255)
                                                                     aaGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaaaa                                                                    >  1:1352800/1‑140 (MQ=255)
                                                                     aaGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaaaa                                                                    >  1:468743/1‑140 (MQ=255)
                                                                     aaGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaaaa                                                                    >  1:2087031/1‑140 (MQ=255)
                                                                     aaGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCAGTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaaaa                                                                    >  1:1553304/1‑140 (MQ=255)
                                                                      aGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAg                                                                   >  2:508645/1‑140 (MQ=255)
                                                                      aGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAg                                                                   >  2:1981343/1‑140 (MQ=255)
                                                                         cATGGGCTACATCTTGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGTACTGGTCTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCTTTTATAAAACGACCAAGCTGAAGCAGGAAAAGGTAGAAAAAGCgg                                                                <  2:1653395/140‑1 (MQ=255)
                                                                            gggCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAg                                                             >  2:129322/1‑140 (MQ=255)
                                                                              gatACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAt                                                           <  2:2237701/138‑1 (MQ=255)
                                                                              gCTACATCTGGGTTCAGGAGCTGGACCAAAATTTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAt                                                           <  2:146890/140‑1 (MQ=255)
                                                                              gCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAt                                                           <  1:256806/140‑1 (MQ=255)
                                                                              gCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAt                                                           <  2:1992608/140‑1 (MQ=255)
                                                                              gCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAt                                                           <  2:2398903/140‑1 (MQ=255)
                                                                              gCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAt                                                           <  2:1422390/140‑1 (MQ=255)
                                                                                  cATCTGGGTTCAGGAGCTGGAGCAAAATCTGACGGAAGAAGTACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAATTCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAGCTCa                                                       >  1:1653395/1‑140 (MQ=255)
                                                                                  cATCTGGGTTCAGGAGCTGGACCCAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGCAAAGGAAGAAAAAGCGGAAGATCTCa                                                       >  2:1635291/1‑140 (MQ=255)
                                                                                  cATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGACAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGACGATCTCa                                                       >  2:1517513/1‑140 (MQ=255)
                                                                                  cATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCa                                                       >  2:166829/1‑140 (MQ=255)
                                                                                  cATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCa                                                       >  2:804740/1‑140 (MQ=255)
                                                                                  cATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCa                                                       >  1:147326/1‑140 (MQ=255)
                                                                                  cATCTGGGTTCAGGACCTGGACCAACATCTGACGGACGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCTTGTCGGGAAAATCATTTATAAAACGAACAAGCGGCCGCAGGCAAAGGCCGAAAAAGCGGAACATCTCa                                                       >  2:1310169/1‑140 (MQ=255)
                                                                                       gggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTaaa                                                  <  1:588675/140‑1 (MQ=255)
                                                                                       gggTTCAGGAGCTGGAACAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTaaa                                                  <  1:226394/140‑1 (MQ=255)
                                                                                        ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGACAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAAc                                                 >  2:2323991/1‑140 (MQ=255)
                                                                                        ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaa                                                                      >  2:1298617/1‑119 (MQ=255)
                                                                                        ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAAc                                                 >  2:53412/1‑140 (MQ=255)
                                                                                        ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAAc                                                 >  2:1482957/1‑140 (MQ=255)
                                                                                        ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAAc                                                 >  2:2049134/1‑140 (MQ=255)
                                                                                        ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAAc                                                 >  2:781036/1‑140 (MQ=255)
                                                                                        ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAAc                                                 >  2:1915116/1‑140 (MQ=255)
                                                                                        ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAACGAAGAAAAAGCGGAAGATCACATTAAAc                                                 >  2:416637/1‑140 (MQ=255)
                                                                                           tCAGGAGCTGGACCAAAATCTGAGGGAAGAAGAACTAGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACggg                                              <  2:1357432/140‑1 (MQ=255)
                                                                                           tCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACggg                                              <  2:2182117/140‑1 (MQ=255)
                                                                                            cAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGc                                             <  2:1086800/140‑1 (MQ=255)
                                                                                            cAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGc                                             >  1:1298834/1‑140 (MQ=255)
                                                                                             aGGAGCTGGACCAAAATCTGACGGATGAAGAGCTCGACTTTTTGTACGAGACCTCCTTCCATGTCGGGAAAATCATTTATAAAACGAATAAATTGAAACAGGAAAAGGAAGAAAAAGCAGAGGATCTCATCAAACGGTCg                                            >  2:157467/1‑140 (MQ=255)
                                                                                             aGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGc                                             <  1:1898390/139‑1 (MQ=255)
                                                                                             aGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCg                                            >  1:1185537/1‑140 (MQ=255)
                                                                                             aGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCACGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCg                                            >  2:2378229/1‑140 (MQ=255)
                                                                                              ggAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGa                                           <  2:1943809/140‑1 (MQ=255)
                                                                                              ggAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGa                                           <  2:1943849/140‑1 (MQ=255)
                                                                                                 gCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCt                                        <  2:1185537/140‑1 (MQ=255)
                                                                                                  cTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTa                                       >  2:1698153/1‑140 (MQ=255)
                                                                                                   tGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTAt                                      <  2:1175369/140‑1 (MQ=255)
                                                                                                    ggACCAAAATCTGACGGACGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGAGGAAGATCTCATTAAACGGGCGATCTATc                                     >  2:1599909/1‑140 (MQ=255)
                                                                                                    ggACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATc                                     >  1:781061/1‑140 (MQ=255)
                                                                                                    ggACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGAAGGAAAAGGAAGAAAAAGCGGTAGATCTCATTAAAAGGGAGAtctctc                                     >  2:2035925/1‑140 (MQ=255)
                                                                                                      aCCAAACTCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAg                                   <  2:105420/140‑1 (MQ=255)
                                                                                                       ccAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGc                                  <  1:1365925/140‑1 (MQ=255)
                                                                                                          aaaTCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTTTCAGCAAt                               >  2:1870211/1‑140 (MQ=255)
                                                                                                          aaaTCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAAt                               >  2:1730250/1‑140 (MQ=255)
                                                                                                             tCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTa                            <  1:1244396/140‑1 (MQ=255)
                                                                                                              cTGACGGAAGCAGAACTCGCCTTTTTGTATGAGACCTCTTTTCATGTCGGGAACATCATGTATAAAACGAACACGCTGAAGCAGTAAAAGGAAGACACAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTAc                           <  2:1447331/140‑1 (MQ=255)
                                                                                                                 aCGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCAc                        <  1:1443870/140‑1 (MQ=255)
                                                                                                                 aCGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCAc                        <  2:468743/140‑1 (MQ=255)
                                                                                                                  cGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACt                       <  1:455489/140‑1 (MQ=255)
                                                                                                                      agaagaaCTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTgaga                   <  1:1572318/140‑1 (MQ=255)
                                                                                                                      agaagaaCTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTgaga                   <  1:1383945/140‑1 (MQ=255)
                                                                                                                           aaCTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGaa              <  1:1274719/140‑1 (MQ=255)
                                                                                                                           aaCTCGACTTTTTGTATGAGACCTATTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGaa              <  1:2402241/140‑1 (MQ=255)
                                                                                                                                  cTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGAc       >  1:217623/1‑140 (MQ=255)
                                                                                                                                     tttGTACGAGACCTCCTTCCATGTCGGGAAAATCATTTATAAAACGAATAAATTGAAACAGGAAAAGGAAGAAAAAGCAGAGGATCTCATCAAACGGGCGATCTATCAGCAATTTACCTCTGAGAAGGAACTCAGACggg    <  2:1278619/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                       
TTCGCGTCAATAAAATTGAAAGCATTGGGTTAAACCAGAGGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAA  >  NZ_CP034943/344409‑344683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: