Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,061,293 T→C 7.3% I140I (ATT→ATC hemY → protoporphyrinogen oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,061,2930TC 7.3% 510.6 / 21.4 164I140I (ATT→ATChemYprotoporphyrinogen oxidase
Reads supporting (aligned to +/- strand):  ref base T (94/58);  new base C (6/6);  total (100/64)
Fisher's exact test for biased strand distribution p-value = 5.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 169 total aligned reads displayed.

AATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTGGCGACATTGACAAGCTTAGTC  >  NZ_CP034943/1061154‑1061429
                                                                                                                                           |                                                                                                                                        
aaTCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCAtt                                                                                                                                          >  1:22141/1‑140 (MQ=255)
aaTCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCAtt                                                                                                                                          >  1:2374454/1‑140 (MQ=255)
aaTCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCAtt                                                                                                                                          >  1:1544778/1‑140 (MQ=255)
aaTCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCAtt                                                                                                                                          <  2:1205146/140‑1 (MQ=255)
  tCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTct                                                                                                                                        >  1:2087014/1‑140 (MQ=255)
       tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGACGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                                                   >  2:301882/1‑140 (MQ=255)
       tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                                                   >  1:932462/1‑140 (MQ=255)
       tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                                                   >  2:1860849/1‑140 (MQ=255)
       tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                                                   >  1:961574/1‑140 (MQ=255)
       tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                                                   >  1:26051/1‑140 (MQ=255)
       tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                                                   >  2:1466940/1‑140 (MQ=255)
       tgtgCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTctgcctg                                                                                                                                   >  1:1060441/1‑140 (MQ=255)
           cTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaag                                                                                                                               >  1:594998/1‑140 (MQ=255)
           cTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaag                                                                                                                               >  2:2192099/1‑140 (MQ=255)
            ttGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaagc                                                                                                                              >  2:323262/1‑140 (MQ=255)
            ttGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaagc                                                                                                                              <  1:504630/140‑1 (MQ=255)
            ttGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaagc                                                                                                                              >  1:1527111/1‑140 (MQ=255)
            ttGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaagc                                                                                                                              <  2:833307/140‑1 (MQ=255)
            ttGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaagc                                                                                                                              >  2:879711/1‑140 (MQ=255)
            ttGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTGTCTGTGTCTGGGAAGGCACGGGCCGCTATGGATTTCATTCTGCCTgcaagc                                                                                                                              >  1:1286644/1‑140 (MQ=255)
             tGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCTGTAATGGGGATACCGACAAAAATTGCGCCGTTTGTTTCTACGGGTCTGTTTTCCTTGTCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTgcaagca                                                                                                                             >  2:1096180/1‑140 (MQ=255)
             tGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCTGTAATGGGGATACCGACAAAAATTGCGCCGTTTGTTTCTACGGGTCTGTTTTCCTTGTCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTgcaagca                                                                                                                             >  2:1004191/1‑140 (MQ=255)
             tGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTgcaagca                                                                                                                             >  1:257340/1‑140 (MQ=255)
                      cACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAg                                                                                                                    >  1:490189/1‑140 (MQ=255)
                      cACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAg                                                                                                                    >  2:1210536/1‑140 (MQ=255)
                           tGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAgacgac                                                                                                               <  1:367092/140‑1 (MQ=255)
                           tGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAgacgac                                                                                                               <  1:1027344/140‑1 (MQ=255)
                           tGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAgacgac                                                                                                               <  1:2192099/140‑1 (MQ=255)
                                    tGCCGACGGGCGCCGGGAGGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTg                                                                                                      >  2:533090/1‑140 (MQ=255)
                                    tGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTGTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTg                                                                                                      >  2:1142292/1‑140 (MQ=255)
                                       cGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGga                                                                                                   >  2:533904/1‑140 (MQ=255)
                                       cGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGga                                                                                                   >  2:771624/1‑140 (MQ=255)
                                              cgcCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAAtttt                                                                                            >  1:700619/1‑140 (MQ=255)
                                                ccGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACTACCAGTCATTGGGAGAATTTTTc                                                                                          >  2:2155409/1‑140 (MQ=255)
                                                ccGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTGTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTc                                                                                          >  1:1227493/1‑140 (MQ=255)
                                                ccGTGATGGGGATACCGACAAAAATTGCGCCCTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTc                                                                                          >  2:725157/1‑140 (MQ=255)
                                                 cGTGATGGGGATACCGACAACAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTcc                                                                                         <  2:1110933/140‑1 (MQ=255)
                                                     aTGGGGATACCGACAAAACTTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAg                                                                                     <  1:2364277/140‑1 (MQ=255)
                                                           aTACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCgtgt                                                                               <  1:1573014/140‑1 (MQ=255)
                                                           aTACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCgtgt                                                                               <  1:58430/140‑1 (MQ=255)
                                                             aCCGACAACAAGTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCg                                                                             <  2:2343075/140‑1 (MQ=255)
                                                             aCCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCg                                                                             >  2:1042226/1‑140 (MQ=255)
                                                             aCCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCg                                                                             >  2:2149283/1‑140 (MQ=255)
                                                               cGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGga                                                                           >  2:1983039/1‑140 (MQ=255)
                                                               cGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCAGCAGGCGTGTCGga                                                                           >  2:423091/1‑140 (MQ=255)
                                                               cGACAAAAATAGCTCCTATAGTTTCAACCAGTCTGTTTTCTGTTTATGGTAAGGCAATGGCGCCTATTCATTTAATTCTGCATGCAAGCAAGTCAAAAGACGAACAGTAATTGCGATAATATTTCAGCAGCAgtgtatga                                                                           >  2:1469948/1‑136 (MQ=255)
                                                                   aaaaaTTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATg                                                                       >  2:2191518/1‑140 (MQ=255)
                                                                     aaaTTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGaa                                                                     <  1:1146440/140‑1 (MQ=255)
                                                                     aaaTTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGaa                                                                     <  1:1210536/140‑1 (MQ=255)
                                                                           cgcCTTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAATGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGAAGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                                               <  1:1598237/140‑1 (MQ=255)
                                                                           cgcCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGGAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                                               <  1:10975/140‑1 (MQ=255)
                                                                           cgcCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                                               <  1:1001897/140‑1 (MQ=255)
                                                                           cgcCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                                               <  1:1892671/140‑1 (MQ=255)
                                                                           cgcCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                                               <  1:1598049/140‑1 (MQ=255)
                                                                           cgcCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                                               <  2:1639893/140‑1 (MQ=255)
                                                                           cgcCGGTCGTTTCAACCGGTCTGTTTACTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGtt                                                               <  1:1306992/140‑1 (MQ=255)
                                                                                      tCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAAt                                                    <  2:25111/140‑1 (MQ=255)
                                                                                          ccGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAg                                                <  2:1651755/140‑1 (MQ=255)
                                                                                          ccGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAg                                                <  2:132439/140‑1 (MQ=255)
                                                                                          ccGGTCTGTTTTCGGTGTCGGGGAAGGCACGGGCCGCTATGGATTTCATTCTGCCTGCACGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGAGGAAGTGGTTGAGAATTTAATCGAg                                                <  2:1880338/140‑1 (MQ=255)
                                                                                          ccGGGCTGTATTCTGTGTCTGGGAACGCACCGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGATAATTTTTCCGCAGGCGGGTCGGAGATGAAGTGGTTGAGAATTTAATCGAg                                                <  2:319337/140‑1 (MQ=255)
                                                                                             gTCTGTTTTCTGTGTCTGGGAAGGCAATGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACAAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGccc                                             >  1:2170403/1‑139 (MQ=255)
                                                                                             gTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGc                                                                                   >  1:1584369/1‑102 (MQ=255)
                                                                                             gTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGc                                                                                   <  2:1584369/102‑1 (MQ=255)
                                                                                             gTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCg                                             >  1:1182142/1‑140 (MQ=255)
                                                                                             gTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCg                                             >  1:2316172/1‑140 (MQ=255)
                                                                                             gTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGAAGAAGTGGTTGAGAATTTAATCGAGCCg                                             >  2:996408/1‑140 (MQ=255)
                                                                                              tCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGc                                            >  2:1945099/1‑140 (MQ=255)
                                                                                                    ttCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTAt                                      <  1:635293/140‑1 (MQ=255)
                                                                                                    ttCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTAt                                      <  1:2317881/140‑1 (MQ=255)
                                                                                                      ccttgtCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTGCAAGCAAAACAAAGGATGATCAGTCATTGGGAGAATTCTTCCGCAGACGTGTCGGAGATGAAGTGGTCGAGAACTTAATCGAGCCGCTACTATCa                                    <  1:878510/137‑1 (MQ=255)
                                                                                                      ccttgtCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTGCAAGCAAAACAAAGGATGATCAGTCATTGGGAGAATTCTTCCGCAGACGTGTCGGAGATGAAGTGGTCGAGAACTTAATCGAGCCGCTACTATCa                                    <  1:1004191/137‑1 (MQ=255)
                                                                                                      ccttgtCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTGCAAGCAAAACAAAGGATGATCAGTCATTGGGAGAATTCTTCCGCAGACGTGTCGGAGATGAAGTGGTCGAGAACTTAATCGAGCCGCTACTATCa                                    <  1:1096180/137‑1 (MQ=255)
                                                                                                      ccttgtCCGGGAAGGCGAGAGCTGCTATGGATTTCATCCTGCCTGCAAGCAAAACAAAGGATGATCAGTCATTGGGAGAATTCTTCCGCAGACGTGTCGGAGATGAAGTGGTCGAGAACTTAATCGAGCCGCTACTATCa                                    <  1:1112546/137‑1 (MQ=255)
                                                                                                      cTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCa                                    <  1:2149283/140‑1 (MQ=255)
                                                                                                      cTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCa                                    <  2:191059/140‑1 (MQ=255)
                                                                                                       tgtgTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAg                                   >  1:1763990/1‑140 (MQ=255)
                                                                                                       tgtgTCTGGGAAGGCAAGGGCCGATATGGATTTCATTATGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAg                                   >  1:2314591/1‑140 (MQ=255)
                                                                                                          gtCTGGGAACGCAAGGGCCGCTAAGGATTTCATTCTGCCTGCAAGCAACTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCa                                <  1:1286449/140‑1 (MQ=255)
                                                                                                               ggAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGc                                               >  2:2390896/1‑120 (MQ=255)
                                                                                                               ggAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGc                                               <  1:2390896/120‑1 (MQ=255)
                                                                                                                gAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACg                          <  1:1679511/140‑1 (MQ=255)
                                                                                                                   ggCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTg                       >  1:59736/1‑140 (MQ=255)
                                                                                                                     cAAGGGCCGCTATGGATTTCATTCTGCCTGCACGCAAGTCAAAAGACGAACAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAAATTAATCGAGCCGCTTCTATCAGGCAACTACGCTGGc                     >  2:2301512/1‑140 (MQ=255)
                                                                                                                     cAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTGGc                     >  2:453999/1‑140 (MQ=255)
                                                                                                                       aGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTGGCGa                   >  1:1743431/1‑140 (MQ=255)
                                                                                                                         ggCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTGGCGACa                 >  2:732474/1‑140 (MQ=255)
                                                                                                                         ggCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTCGCGACa                 >  2:2060769/1‑140 (MQ=255)
                                                                                                                              cTATGGATTTCATTCTGCCTTCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGACTTTAATCGAGCCGCTTCTATCAGGCATCTACGCTGGCCACCTTGAc            >  2:678487/1‑140 (MQ=255)
                                                                                                                              cTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTGGCGACATTGAc            >  1:2928/1‑140 (MQ=255)
                                                                                                                              cTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTGGCGACATTGAc            <  2:531610/140‑1 (MQ=255)
                                                                                                                              cTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTGGCGACATTGAc            <  2:2087014/140‑1 (MQ=255)
                                                                                                                              cTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTGGCGACATTGAc            >  1:1052882/1‑140 (MQ=255)
                                                                                                                                   gATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTGGCGACATTGACAAGCt       <  1:649961/140‑1 (MQ=255)
                                                                                                                                   gATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTGGCGACATTGACAAGCt       <  1:1945099/140‑1 (MQ=255)
                                                                                                                                   gATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTGGCGACATTGACAAGCt       <  2:2334280/140‑1 (MQ=255)
                                                                                                                                      ttCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTGGCGACATTGACAAGCTTAg    >  2:631421/1‑140 (MQ=255)
                                                                                                                                      ttCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTGGCGACATTGACAAGATTAg    >  2:1699468/1‑140 (MQ=255)
                                                                                                                                        cATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAATTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTAAGCTGGCGACATTGACAAGCTTAGTc  >  2:2171471/1‑140 (MQ=255)
                                                                                                                                         aTTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAg                                                >  1:532418/1‑93 (MQ=255)
                                                                                                                                           |                                                                                                                                        
AATCCTATGTGCTTGTAAACCGCACCCTGCATCCAATGCCGAAGGGCGCCGTGATGGGGATACCGACAAAAATTGCGCCGTTCGTTTCAACCGGTCTGTTTTCTGTGTCTGGGAAGGCAAGGGCCGCTATGGATTTCATTCTGCCTGCAAGCAAGTCAAAAGACGACCAGTCATTGGGAGAATTTTTCCGCAGGCGTGTCGGAGATGAAGTGGTTGAGAATTTAATCGAGCCGCTTCTATCAGGCATCTACGCTGGCGACATTGACAAGCTTAGTC  >  NZ_CP034943/1061154‑1061429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: