Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,196,397 A→G 5.0% K110K (AAA→AAG EO946_RS06155 → GNAT family N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,196,3970AG 5.0% 319.9 / 5.0 99K110K (AAA→AAGEO946_RS06155GNAT family N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (31/63);  new base G (2/3);  total (33/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01

GGCAGGGGCTTGGCGGCAAATTGATCGCGAAGCTGAAAAAGAAAAATAAGCCGATATTGCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCAGAAGAAGAAATTCTTGAAGCCATGAAACAGACATATGAAAA  >  NZ_CP034943/1196261‑1196531
                                                                                                                                        |                                                                                                                                      
ggCAGGGGCTTGGCGGCAAATTGATCGCGAAGCTGAAAAAGAAAAATAAGCCGATATTGCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACAt                                                                                                                                     >  1:431997/1‑140 (MQ=255)
  cAGGGGCTTGGCGGCAAATTGATCGCGAAGCTGAAAAAGAAAAATAAGCCGATATTGCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGc                                                                                                                                   >  1:393341/1‑140 (MQ=255)
    ggggCTTGGCGGCAAATTGATCGCGAAGCTGAAAAAGAAAAATAAGCCGATATTGCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTc                                                                                                                                 <  2:995322/140‑1 (MQ=255)
    ggggCTTGGCGGCAAATTGATCGCGAAGCTGAAAAAGAAAAATAAGCCGATATTGCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTc                                                                                                                                 <  2:1220435/140‑1 (MQ=255)
    ggggCTTGGCGGCAAATTGATCGCGAAGCTGAAAAAGAAAAATAAGCCGATATTGCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTc                                                                                                                                 <  2:1032038/140‑1 (MQ=255)
    ggggCTTGGCGGCAAATTGATCGCGAAGCTGAAAAAGAAAAATAAGCCGATATTGCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTc                                                                                                                                 <  2:1652866/140‑1 (MQ=255)
    ggggCTTGGCGGCAAATTGATCGCGAAGCTGAAAAAGAAAAATAAGCCGATATTGCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTc                                                                                                                                 <  1:366155/140‑1 (MQ=255)
    ggggCTTGGCGGCAAATTGATCGCGAAGCTGAAAAAGAAAAATAAGCCGATATTGCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTc                                                                                                                                 <  2:1904760/140‑1 (MQ=255)
    ggggCTTGGCGGCAAATTGATCGCGAAGCTGAAAAAGAAAAATAAGCCGATATTGCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTc                                                                                                                                 <  1:1342716/140‑1 (MQ=255)
     gggCTTGGCGGCAAATTGATCGCGAAGCTGAAAAATAAAAATAAGCCGATATTGCTCGATGTGGAGCCTGTTGATTAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCa                                                                                                                                <  1:2262112/140‑1 (MQ=255)
     gggCTTGGCGGCAAATTGATCGCGAAGCTGAAAAAGAAAAATAAGCCGATATTGCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCa                                                                                                                                <  2:150947/140‑1 (MQ=255)
     gggCTTGGCGGCAAATTGATCGCGAAGCTGAAAAAGAAAAATAAGCCGATATTGCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCa                                                                                                                                <  2:1751055/140‑1 (MQ=255)
     gggCTTGGCGGCAAATTGATCGCGAAGCTGAAAAAGAAAAATAAGCCGATATTGCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCa                                                                                                                                <  2:2068571/140‑1 (MQ=255)
       gCTTTGCGGCCAAATGAGAGCGACGGTGAATCACAAATATAAGCCGATATTGCTCTACGTGGAGCCTGTGGCTGAAGATGATACTGATACAGAAAAACGCGTGAGGTTTTATCAATGAGATCATTTCAAACATGCTCAGt                                                                                                                              <  1:1435672/140‑1 (MQ=255)
       gCTTGGCGGCAAATTGATCGCGAAGCTGAAAAAGAAAAATAAGCCGATATTGCTCTAGGTGGAGCCTGTGGATTAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGt                                                                                                                              <  1:1213464/140‑1 (MQ=255)
       gCTTGGCGGCAAATTGATCGCGAAGCTGAAAAAGAAAAATAAGCCGATATTGCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGt                                                                                                                              <  1:1609915/140‑1 (MQ=255)
       gCTTGGCGGCAAATTGATCGCGAAGCTGAAAAAGAAAAATAAGCCGATATTGCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGt                                                                                                                              <  2:2212480/140‑1 (MQ=255)
       gCTTGGCGGCAAATTGATCGCGAAGCTGAAAAAGAAAAATAAGCCGATATTGCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGt                                                                                                                              <  2:67331/140‑1 (MQ=255)
          tGGCGGCAAATTGATCGCGAAGCTGAAAAAGAAAAATAAGCCGATATTGCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCaa                                                                                                                           <  1:2363265/140‑1 (MQ=255)
          tGGCGGCAAATTGATCGCGAAGCTGAAAAAGAAAAATAAGCCGATATTGCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCaa                                                                                                                           >  2:1517022/1‑140 (MQ=255)
                  aaTTGATCGCGAAGCTGAAAAAGAAAAATAAGCCGATATTGCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTAc                                                                                                                   <  1:1715201/140‑1 (MQ=255)
                     ggATCGCGAAGCTGAAAAAGAAAAATAAGCCGATATTGCTCGAGGTGGAGCCTGTGGATGAAGATGAGACTGCTACAGAAAAACGCCTGAGGTTTTATCAACGAGAGCATTTCAAACATGCTCAGTCACTTGGGTACCgg                                                                                                                <  2:1124480/139‑1 (MQ=255)
                          gcgAAGCTGAAAAAGAAAAATAAGCCGATATTGCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCg                                                                                                           <  2:2298441/140‑1 (MQ=255)
                                   aaaaagaaaaaTAAGCCGATATTGCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGc                                                                                                  <  1:1229809/140‑1 (MQ=255)
                                         aaaaaTAAGCCGATATTGCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGaa                                                                                            <  2:2147352/140‑1 (MQ=255)
                                         aaaaaTAAGCCGATATTGCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGaa                                                                                            <  2:2151855/140‑1 (MQ=255)
                                             aTAAGCCGATATTGCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGaa                                                                                        <  1:1165308/140‑1 (MQ=255)
                                             aTAAGCCGATATTGCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCAATGGCTACGAATGaa                                                                                        <  1:2310462/140‑1 (MQ=255)
                                                   cGATATTGCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAAc                                                                                  <  1:1517022/140‑1 (MQ=255)
                                                          gCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATgg                                                                           <  2:350358/140‑1 (MQ=255)
                                                          gCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATgg                                                                           <  2:1818705/140‑1 (MQ=255)
                                                          gCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATgg                                                                           <  2:2177282/140‑1 (MQ=255)
                                                          gCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATgg                                                                           <  2:1709705/140‑1 (MQ=255)
                                                          gCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATgg                                                                           <  2:1713527/140‑1 (MQ=255)
                                                          gCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATgg                                                                           <  2:1710021/140‑1 (MQ=255)
                                                           cTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATCga                                                                          >  2:1503059/1‑140 (MQ=255)
                                                             cGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGgaga                                                                        <  2:129671/140‑1 (MQ=255)
                                                                   ggAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGt                                                                  <  1:1762056/140‑1 (MQ=255)
                                                                   ggAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGt                                                                  <  1:2190091/140‑1 (MQ=255)
                                                                    gAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTa                                                                 <  2:242113/140‑1 (MQ=255)
                                                                    gAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTa                                                                 <  2:1459445/140‑1 (MQ=255)
                                                                     aGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTAc                                                                >  2:315755/1‑140 (MQ=255)
                                                                     aGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTAc                                                                >  2:780294/1‑140 (MQ=255)
                                                                        cTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAGCATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTgg                                                             <  2:2176260/140‑1 (MQ=255)
                                                                        cTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTgg                                                             <  1:780294/140‑1 (MQ=255)
                                                                        cTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTgg                                                             <  1:315755/140‑1 (MQ=255)
                                                                        cTGTGGATGAAGATGATACTGAGACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAGCATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTgg                                                             <  2:1722568/140‑1 (MQ=255)
                                                                         tgtgGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGt                                                            >  1:2361084/1‑140 (MQ=255)
                                                                         ggtgGATGAAGATGAGACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGt                                                            >  1:105166/2‑140 (MQ=255)
                                                                          gtgGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTc                                                           >  2:194186/1‑140 (MQ=255)
                                                                           tgGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCGCAGAGAGCATTTCAAACATGCTCAGTCACTGGGGTAGCGGCGCCGGGGATTGGCTACGAATGACGTCATCAGGGTGGAGATTGTGTACTggggc                                                          >  2:1831516/1‑137 (MQ=255)
                                                                                     tgatactgatacAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAg                                                >  2:956316/1‑140 (MQ=255)
                                                                                      gatactgatacAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGa                                               >  2:2191808/1‑140 (MQ=255)
                                                                                       atactgatacAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAGCATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGaa                                              >  1:710886/1‑140 (MQ=255)
                                                                                       atactgatacAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGaa                                              >  2:1504578/1‑140 (MQ=255)
                                                                                       atactgatacAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGaa                                              >  2:37521/1‑140 (MQ=255)
                                                                                       atactgatacAGAAAAACGCCTGAGGGTTTAACAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGaa                                              >  1:2251308/1‑140 (MQ=255)
                                                                                        tactgatacAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAAt                                             >  2:889171/1‑140 (MQ=255)
                                                                                           tgatacAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCag                                          <  1:975785/140‑1 (MQ=255)
                                                                                           tgatacAGAAAAACGCCTGAGGATTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCag                                          <  1:1622391/140‑1 (MQ=255)
                                                                                            gatacAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTACACAAGATGGAGATTCTGTAATGGTCACCAAAAACAGAATCaga                                         <  2:182573/140‑1 (MQ=255)
                                                                                            gatacAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCaga                                         <  2:467305/140‑1 (MQ=255)
                                                                                            gatacAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCaga                                         <  2:964074/140‑1 (MQ=255)
                                                                                             atacAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGgaga                                                                        <  1:1902155/108‑1 (MQ=255)
                                                                                             atacAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGgaga                                                                        >  2:1902155/1‑108 (MQ=255)
                                                                                             atacAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCagaa                                        >  1:137476/1‑140 (MQ=255)
                                                                                             atacAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCagaa                                        >  1:2345852/1‑140 (MQ=255)
                                                                                             atacAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCagaa                                        >  2:1153405/1‑140 (MQ=255)
                                                                                             atacAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCagaa                                        >  1:975513/1‑140 (MQ=255)
                                                                                             atacAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCagaa                                        >  1:825377/1‑140 (MQ=255)
                                                                                             atacAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCagaa                                        >  1:57134/1‑140 (MQ=255)
                                                                                             atacAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCagaa                                        >  1:459549/1‑140 (MQ=255)
                                                                                             atacAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACAGGCTCAGTCACGTGGGTCCCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCGGTACTGGTCACCAACAACAGAATCagaa                                        >  1:554672/1‑140 (MQ=255)
                                                                                             atacAGAAAAACGCCTGAGGTTTTAACAAAGAGATCATTTCAAACATGATCAGTCAATTGGTTACCGGCGCCGGTCATTGGCTACGAATTAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAAtaataa                                        >  1:2083972/1‑135 (MQ=255)
                                                                                              tacAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCagaag                                       >  2:482592/1‑140 (MQ=255)
                                                                                              tacAGAAAAACGCCGGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCagaag                                       >  2:698044/1‑140 (MQ=255)
                                                                                               acAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCACTTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCagaaga                                      <  2:1306090/140‑1 (MQ=255)
                                                                                               acAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCagaaga                                      <  2:1258541/140‑1 (MQ=255)
                                                                                                   aaaaaCGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCACTTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCAgaagaagaa                                  >  1:816652/1‑140 (MQ=255)
                                                                                                   aaaaaCGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCAgaagaagaa                                  >  2:182031/1‑140 (MQ=255)
                                                                                                           cTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCAGAAGAAGAAATTCTTGa                          <  1:1772230/140‑1 (MQ=255)
                                                                                                                gTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCAGAAGAAGAAATTCTTGAAGCCa                     <  1:1718730/140‑1 (MQ=255)
                                                                                                                   ttATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCAGAAGAAGAAATTCTTGAAGCCATGa                  >  1:853112/1‑140 (MQ=255)
                                                                                                                     aTCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCAGAAGAAGAAATTCTTGAAGCCATGaaa                <  1:194186/140‑1 (MQ=255)
                                                                                                                       cAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCAGAAGAAGAAATTCTTGAAGCCATGAAACa              <  2:213458/140‑1 (MQ=255)
                                                                                                                       cAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCAGAAGAAGAAATTCTTGAAGCCATGAAACa              <  1:1504578/140‑1 (MQ=255)
                                                                                                                       cAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCAGAAGAAGAAATTCTTGAAGCCATGAAACa              <  2:213306/140‑1 (MQ=255)
                                                                                                                       cAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCAGAAGAAGAAATTCTTGAAGCCATGAAACa              <  1:37521/140‑1 (MQ=255)
                                                                                                                       cAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCAGAAGAAGAAATTCTTGAAGCCATGAAACa              <  2:1821774/140‑1 (MQ=255)
                                                                                                                       cAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCAGAAGAAGAAATTCTTGAAGCCATGAAACa              <  2:819590/140‑1 (MQ=255)
                                                                                                                       cAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCAGAAGAAGAAATTCTTGAAGCCATGAAACa              <  2:819664/140‑1 (MQ=255)
                                                                                                                       cAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCAGAAGAAGAAATTCTTGAAGCCATGAAACa              <  2:1865273/140‑1 (MQ=255)
                                                                                                                         aaGAGAGCATTTGAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACTAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCAGAAGAAGAAATTCTTGAAGCCATGAAACAGa            <  1:1418936/140‑1 (MQ=255)
                                                                                                                         aaGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCAGAAGAAGAAATTCTTGAAGCCATGAAACAGa            <  1:1285250/140‑1 (MQ=255)
                                                                                                                                 aTTTCAAGCATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCAGAAGAAGAAATTCTTGAAGCCATGAAACAGACATATGaa    <  2:1771130/140‑1 (MQ=255)
                                                                                                                                 aTTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCAGAAGAAGAAATTCTTGAAGCCATGAAACAGACATATGaa    <  1:1028949/140‑1 (MQ=255)
                                                                                                                                   ttCAAGCATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCAGAAGAAGAAATTCTTGAAGCCATGAAACAGACATATGaaaa  >  2:2036704/1‑140 (MQ=255)
                                                                                                                                   ttCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCAGAAGAAGAAATTCTTGAAGCCATGAAACAGACATATGaaaa  <  1:163958/140‑1 (MQ=255)
                                                                                                                                   ttCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCAGAAGAAGAAATTCTTGAAGCCATGAAACAGACATATGaaaa  <  1:2368457/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
GGCAGGGGCTTGGCGGCAAATTGATCGCGAAGCTGAAAAAGAAAAATAAGCCGATATTGCTCGAGGTGGAGCCTGTGGATGAAGATGATACTGATACAGAAAAACGCCTGAGGTTTTATCAAAGAGAGCATTTCAAACATGCTCAGTCAATTGGGTACCGGCGCCGGTCATTGGCTACGAATGAAGTAAACAAGATGGAGATTCTGTACTGGTCACCAAAAACAGAATCAGAAGAAGAAATTCTTGAAGCCATGAAACAGACATATGAAAA  >  NZ_CP034943/1196261‑1196531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: