Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,268,698 T→A 6.4% intergenic (+8/‑192) EO946_RS06595 → / → uxaC NAD(P)/FAD‑dependent oxidoreductase/glucuronate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,268,6980TA 6.4% 318.7 / 10.1 94intergenic (+8/‑192)EO946_RS06595/uxaCNAD(P)/FAD‑dependent oxidoreductase/glucuronate isomerase
Reads supporting (aligned to +/- strand):  ref base T (47/41);  new base A (3/3);  total (50/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 105 total aligned reads displayed.

GCAAAGACGCGGTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGTAAAATAAAAATA  >  NZ_CP034943/1268560‑1268837
                                                                                                                                          |                                                                                                                                           
gCAAAGACGCGGTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTtg                                                                                                                                            <  2:2018541/140‑1 (MQ=255)
 cAAAGACGCGGTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTtgt                                                                                                                                           <  2:2385492/140‑1 (MQ=255)
 cAAAGACGCGGTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTtgt                                                                                                                                           <  2:1905111/140‑1 (MQ=255)
 cAAAGACGCGGTTGCCATCATTGGTGCAAATTCTACACCGCTGACAGGTCTTCCGGCTTCCTTGATGACAGAAGCAAGTAACGTACGCTACTTAACTCACAGTAAAGGACTTTTCAGCCTGGCTTACTAATCCGTTTtgt                                                                                                                                           <  1:2336579/140‑1 (MQ=255)
           gTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGAc                                                                                                                                 <  2:1001528/140‑1 (MQ=255)
           gTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGAc                                                                                                                                 <  2:744100/140‑1 (MQ=255)
           gTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGAc                                                                                                                                 <  2:321500/140‑1 (MQ=255)
           gTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGAc                                                                                                                                 <  2:1680290/140‑1 (MQ=255)
           gTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGAc                                                                                                                                 <  2:1685163/140‑1 (MQ=255)
           gTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGAc                                                                                                                                 <  2:2288541/140‑1 (MQ=255)
           gTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGAc                                                                                                                                 <  2:2121562/140‑1 (MQ=255)
           gTTGCCATCATTGGTGCAAAGTCTACACCGCTTAAAGGTCGTCCTGCTTCCTGGATCACAGAAGCAAGTAACGTACGCGACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGAc                                                                                                                                 <  2:339263/140‑1 (MQ=255)
                 atcatTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACCTTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGcc                                                                                                                           <  2:606683/140‑1 (MQ=255)
                 atcatTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGcc                                                                                                                           <  2:298926/140‑1 (MQ=255)
                 atcatTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGCACATTGcc                                                                                                                           <  2:1609886/140‑1 (MQ=255)
                 atcatTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTATTTGTGAACTCGGACATTGcc                                                                                                                           <  2:388495/140‑1 (MQ=255)
                   catTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCaa                                                                                                                         <  1:1538333/140‑1 (MQ=255)
                   catTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCaa                                                                                                                         <  1:1252852/140‑1 (MQ=255)
                   catTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCaa                                                                                                                         <  1:2197981/140‑1 (MQ=255)
                        gTGCAAATTCTCCACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTATTGTGAACTCGGACATTGCCAAAAgtg                                                                                                                    <  1:213088/140‑1 (MQ=255)
                        gTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAgtg                                                                                                                    <  2:2366464/140‑1 (MQ=255)
                            aaaTTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAGGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTcc                                                                                                                <  2:1779655/140‑1 (MQ=255)
                                   acacCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCggg                                                                                                         <  1:328395/140‑1 (MQ=255)
                                    cacCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAATGTACGATATTTGACACATATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTAATGAATCTGGCATTGCCAAAAGCATTCCTTTccgagt                                                                                                       >  2:1981214/1‑135 (MQ=255)
                                    cacCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAATGTACGATATTTGACACATATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTAATGAATCTGGCATTGCCAAAAGCATTCCTTTccgagt                                                                                                       >  2:1874139/1‑135 (MQ=255)
                                    cacCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAATGTACGATATTTGACACATATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTAATGAATCTGGCATTGCCAAAAGCATTCCTTTccgagt                                                                                                       >  2:1683706/1‑135 (MQ=255)
                                    cacCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGa                                                                                                        <  1:539588/140‑1 (MQ=255)
                                    cacCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGa                                                                                                        <  1:787256/140‑1 (MQ=255)
                                    cacCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGa                                                                                                        <  1:2075838/140‑1 (MQ=255)
                                         cTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCtt                                                                                                   >  2:375062/1‑140 (MQ=255)
                                         cTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCtt                                                                                                   >  1:1370350/1‑140 (MQ=255)
                                         cTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCtt                                                                                                   >  2:1631204/1‑140 (MQ=255)
                                                  cTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAAt                                                                                          <  1:1398782/140‑1 (MQ=255)
                                                  cTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAAt                                                                                          <  1:447508/140‑1 (MQ=255)
                                                   ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGTCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                         >  1:2054837/1‑140 (MQ=255)
                                                   ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGTCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                         >  1:25696/1‑140 (MQ=255)
                                                   ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGTCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                         >  1:25710/1‑140 (MQ=255)
                                                   ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGa                                                                                                        <  2:1865859/125‑1 (MQ=255)
                                                   ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGa                                                                                                        >  1:1865859/1‑125 (MQ=255)
                                                   ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                         >  1:1729249/1‑140 (MQ=255)
                                                   ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                         >  1:1386329/1‑140 (MQ=255)
                                                   ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                         >  1:1474854/1‑140 (MQ=255)
                                                   ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                         >  2:2370962/1‑140 (MQ=255)
                                                   ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                         >  1:1650519/1‑140 (MQ=255)
                                                   ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                         >  2:1888319/1‑140 (MQ=255)
                                                   ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                         >  2:2152710/1‑140 (MQ=255)
                                                   ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                         >  2:1927045/1‑140 (MQ=255)
                                                   ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                         >  1:157544/1‑140 (MQ=255)
                                                   ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                         >  1:894214/1‑140 (MQ=255)
                                                   ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                         >  1:113109/1‑140 (MQ=255)
                                                   ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                         >  1:64070/1‑140 (MQ=255)
                                                   ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                         >  1:660574/1‑140 (MQ=255)
                                                    tCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGt                                                                                        <  2:1370350/140‑1 (MQ=255)
                                                           tCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCtttttt                                                                                 >  1:2148494/1‑140 (MQ=255)
                                                           tCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCtttttt                                                                                 >  1:512596/1‑140 (MQ=255)
                                                           tCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCtttttt                                                                                 >  1:1244088/1‑140 (MQ=255)
                                                                   gaAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTa                                                                         <  2:1476096/140‑1 (MQ=255)
                                                                   gaAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTa                                                                         <  1:700995/140‑1 (MQ=255)
                                                                      agaagCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTg                                                                      >  2:931996/1‑140 (MQ=255)
                                                                      agaagCAAGTAACGTACGCTACTTAACTCACAATAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGTACAGTGACAAAAGTGTTCCTTTCGGGATGCTTTACGGCAATGTCTTTTTTTGTTTTTATTg                                                                      >  2:116609/1‑140 (MQ=255)
                                                                        aagCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCt                                                                    >  2:1322398/1‑140 (MQ=255)
                                                                        aagCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCt                                                                    <  1:2251533/140‑1 (MQ=255)
                                                                              gTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGtt                                                              >  2:1273199/1‑140 (MQ=255)
                                                                              gTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGtt                                                              >  2:1631445/1‑140 (MQ=255)
                                                                              gTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGtt                                                              >  2:1991388/1‑140 (MQ=255)
                                                                                        cTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAAcatttcat                                                    <  2:1244088/140‑1 (MQ=255)
                                                                                        cTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAAcatttcat                                                    <  2:512596/140‑1 (MQ=255)
                                                                                           cTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTCACGTTAACATTTCAtttt                                                 >  1:1633936/1‑140 (MQ=255)
                                                                                           cTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCAtttt                                                 >  1:2001668/1‑140 (MQ=255)
                                                                                           cTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCAtttt                                                 >  1:1254148/1‑140 (MQ=255)
                                                                                           cTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCAtttt                                                 >  1:1497229/1‑140 (MQ=255)
                                                                                           cTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTGTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTGTTATTGCTAACGTTAACATTTCAtttt                                                 >  1:2001611/1‑140 (MQ=255)
                                                                                             tAACTCACATTAAAGGACTTTTCAGACTGGCTTACTAAACCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCAGGCAATGTCTTTTTTTTTTTTTATTGCTAACGTTAACATTTCATTTTCa                                               <  2:1671933/140‑1 (MQ=255)
                                                                                             tAACTCACATTAAAGGACTTGTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCa                                               <  2:1671932/140‑1 (MQ=255)
                                                                                              aaCTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCaa                                              >  1:1605778/1‑140 (MQ=255)
                                                                                                   acaTTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAag                                         >  1:457622/1‑140 (MQ=255)
                                                                                                   acaTTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAag                                         >  1:325443/1‑140 (MQ=255)
                                                                                                   acaTTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAag                                         >  1:2075481/1‑140 (MQ=255)
                                                                                                     aTTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGc                                                                                                                            <  1:1185121/55‑1 (MQ=255)
                                                                                                     aTTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGc                                                                                                                            >  2:1185121/1‑55 (MQ=255)
                                                                                                        aaaGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGAtt                                    <  2:236110/140‑1 (MQ=255)
                                                                                                        aaaGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGAtt                                    <  2:1225926/140‑1 (MQ=255)
                                                                                                        aaaGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGAtt                                    <  2:641350/140‑1 (MQ=255)
                                                                                                             aCTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATcc                               <  2:1650519/140‑1 (MQ=255)
                                                                                                                tttCAGCCTGGCTTACTAATCCTTTTAATGAATCTGGCATTGCCAAAAGCATTCCTTTCCGAGTGCTTTCTGGCAATGTCTTTTTTTATGTTTATGACAGCGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCaaag                          <  1:1683706/140‑4 (MQ=255)
                                                                                                                tttCAGCCTGGCTTACTAATCCTTTTAATGAATCTGGCATTGCCAAAAGCATTCCTTTCCGAGTGCTTTCTGGCAATGTCTTTTTTTATGTTTATGACAGCGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCaaag                          <  1:1874139/140‑4 (MQ=255)
                                                                                                                 ttCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTCACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCaga                           >  1:662280/1‑140 (MQ=255)
                                                                                                                 ttCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCaga                           >  2:545804/1‑140 (MQ=255)
                                                                                                                         ggattACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAAtata                   <  2:1200960/137‑1 (MQ=255)
                                                                                                                         ggCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAAtata                   <  2:948770/140‑1 (MQ=255)
                                                                                                                           cTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATaaa                 >  1:1646355/1‑140 (MQ=255)
                                                                                                                           cTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATaaa                 >  1:2125902/1‑140 (MQ=255)
                                                                                                                                 aaTCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTCACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGtaaa           >  2:976556/1‑140 (MQ=255)
                                                                                                                                 aaTCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGtaaa           >  2:906930/1‑140 (MQ=255)
                                                                                                                                       ttttGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGTtaaaataaaa     >  2:713244/1‑140 (MQ=255)
                                                                                                                                       ttttGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGtaaaataaaa     >  2:1085865/1‑140 (MQ=255)
                                                                                                                                        tttGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGTaaaataaaaa    >  2:1069706/1‑140 (MQ=255)
                                                                                                                                          tgtgAACTCGGACATTGCCAATAGTGTTCCTTTCGGGATGCTTGCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCACAGAAGAGATTGATGCCAGAAGAATATAAAGGTaaaataaaaata  >  1:21108/1‑140 (MQ=255)
                                                                                                                                          tgtgAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGTaaaataaaaata  >  1:685948/1‑140 (MQ=255)
                                                                                                                                          tgtgAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGTaaaataaaaata  >  1:2411384/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                           
GCAAAGACGCGGTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGTAAAATAAAAATA  >  NZ_CP034943/1268560‑1268837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: