Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,268,709 A→G 5.6% intergenic (+19/‑181) EO946_RS06595 → / → uxaC NAD(P)/FAD‑dependent oxidoreductase/glucuronate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,268,7090AG 5.6% 341.4 / 6.4 106intergenic (+19/‑181)EO946_RS06595/uxaCNAD(P)/FAD‑dependent oxidoreductase/glucuronate isomerase
Reads supporting (aligned to +/- strand):  ref base A (55/45);  new base G (3/3);  total (58/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

GTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGTAAAATAAAAATAAATCAAA  >  NZ_CP034943/1268571‑1268844
                                                                                                                                          |                                                                                                                                       
gTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGAc                                                                                                                                        <  2:1680290/140‑1 (MQ=255)
gTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGAc                                                                                                                                        <  2:1685163/140‑1 (MQ=255)
gTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGAc                                                                                                                                        <  2:321500/140‑1 (MQ=255)
gTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGAc                                                                                                                                        <  2:744100/140‑1 (MQ=255)
gTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGAc                                                                                                                                        <  2:2288541/140‑1 (MQ=255)
gTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGAc                                                                                                                                        <  2:2121562/140‑1 (MQ=255)
gTTGCCATCATTGGTGCAAAGTCTACACCGCTTAAAGGTCGTCCTGCTTCCTGGATCACAGAAGCAAGTAACGTACGCGACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGAc                                                                                                                                        <  2:339263/140‑1 (MQ=255)
      atcatTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACCTTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGcc                                                                                                                                  <  2:606683/140‑1 (MQ=255)
      atcatTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGcc                                                                                                                                  <  2:298926/140‑1 (MQ=255)
      atcatTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGCACATTGcc                                                                                                                                  <  2:1609886/140‑1 (MQ=255)
      atcatTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTATTTGTGAACTCGGACATTGcc                                                                                                                                  <  2:388495/140‑1 (MQ=255)
        catTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCaa                                                                                                                                <  1:2197981/140‑1 (MQ=255)
        catTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCaa                                                                                                                                <  1:1538333/140‑1 (MQ=255)
        catTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCaa                                                                                                                                <  1:1252852/140‑1 (MQ=255)
             gTGCAAATTCTCCACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTATTGTGAACTCGGACATTGCCAAAAgtg                                                                                                                           <  1:213088/140‑1 (MQ=255)
             gTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAgtg                                                                                                                           <  2:2366464/140‑1 (MQ=255)
                 aaaTTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAGGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTcc                                                                                                                       <  2:1779655/140‑1 (MQ=255)
                        acacCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCggg                                                                                                                <  1:328395/140‑1 (MQ=255)
                         cacCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAATGTACGATATTTGACACATATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTAATGAATCTGGCATTGCCAAAAGCATTCCTTTccgagt                                                                                                              >  2:1981214/1‑135 (MQ=255)
                         cacCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAATGTACGATATTTGACACATATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTAATGAATCTGGCATTGCCAAAAGCATTCCTTTccgagt                                                                                                              >  2:1874139/1‑135 (MQ=255)
                         cacCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAATGTACGATATTTGACACATATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTAATGAATCTGGCATTGCCAAAAGCATTCCTTTccgagt                                                                                                              >  2:1683706/1‑135 (MQ=255)
                         cacCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGa                                                                                                               <  1:539588/140‑1 (MQ=255)
                         cacCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGa                                                                                                               <  1:787256/140‑1 (MQ=255)
                         cacCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGa                                                                                                               <  1:2075838/140‑1 (MQ=255)
                              cTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCtt                                                                                                          >  2:1631204/1‑140 (MQ=255)
                              cTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCtt                                                                                                          >  2:375062/1‑140 (MQ=255)
                              cTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCtt                                                                                                          >  1:1370350/1‑140 (MQ=255)
                                       cTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAAt                                                                                                 <  1:447508/140‑1 (MQ=255)
                                       cTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAAt                                                                                                 <  1:1398782/140‑1 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGTCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                                >  1:2054837/1‑140 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGTCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                                >  1:25696/1‑140 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGTCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                                >  1:25710/1‑140 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGa                                                                                                               <  2:1865859/125‑1 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGa                                                                                                               >  1:1865859/1‑125 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                                >  1:660574/1‑140 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                                >  1:64070/1‑140 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                                >  1:894214/1‑140 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                                >  2:2152710/1‑140 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                                >  2:1927045/1‑140 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                                >  2:1888319/1‑140 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                                >  2:1512847/1‑140 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                                >  1:1729249/1‑140 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                                >  1:1386329/1‑140 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                                >  1:1474854/1‑140 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                                >  1:113109/1‑140 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                                >  1:1650519/1‑140 (MQ=255)
                                        ttCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATg                                                                                                >  1:157544/1‑140 (MQ=255)
                                         tCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGt                                                                                               <  2:1370350/140‑1 (MQ=255)
                                                tCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCtttttt                                                                                        >  1:512596/1‑140 (MQ=255)
                                                tCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCtttttt                                                                                        >  1:2148494/1‑140 (MQ=255)
                                                tCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCtttttt                                                                                        >  1:1244088/1‑140 (MQ=255)
                                                        gaAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTa                                                                                <  1:700995/140‑1 (MQ=255)
                                                        gaAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTa                                                                                <  2:1476096/140‑1 (MQ=255)
                                                           agaagCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTg                                                                             >  2:931996/1‑140 (MQ=255)
                                                           agaagCAAGTAACGTACGCTACTTAACTCACAATAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGTACAGTGACAAAAGTGTTCCTTTCGGGATGCTTTACGGCAATGTCTTTTTTTGTTTTTATTg                                                                             >  2:116609/1‑140 (MQ=255)
                                                             aagCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCt                                                                           <  1:2251533/140‑1 (MQ=255)
                                                             aagCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCt                                                                           >  2:1322398/1‑140 (MQ=255)
                                                                   gTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGtt                                                                     >  2:1991388/1‑140 (MQ=255)
                                                                   gTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGtt                                                                     >  2:1631445/1‑140 (MQ=255)
                                                                   gTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGtt                                                                     >  2:1273199/1‑140 (MQ=255)
                                                                             cTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAAcatttcat                                                           <  2:1244088/140‑1 (MQ=255)
                                                                             cTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAAcatttcat                                                           <  2:512596/140‑1 (MQ=255)
                                                                                cTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTCACGTTAACATTTCAtttt                                                        >  1:1633936/1‑140 (MQ=255)
                                                                                cTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCAtttt                                                        >  1:1497229/1‑140 (MQ=255)
                                                                                cTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCAtttt                                                        >  1:514806/1‑140 (MQ=255)
                                                                                cTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCAtttt                                                        >  1:2001668/1‑140 (MQ=255)
                                                                                cTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCAtttt                                                        >  1:1254148/1‑140 (MQ=255)
                                                                                cTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTGTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTGTTATTGCTAACGTTAACATTTCAtttt                                                        >  1:2001611/1‑140 (MQ=255)
                                                                                  tAACTCACATTAAAGGACTTTTCAGACTGGCTTACTAAACCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCAGGCAATGTCTTTTTTTTTTTTTATTGCTAACGTTAACATTTCATTTTCa                                                      <  2:1671933/140‑1 (MQ=255)
                                                                                   aaCTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCaa                                                     >  1:1605778/1‑140 (MQ=255)
                                                                                        acaTTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAag                                                >  1:2075481/1‑140 (MQ=255)
                                                                                        acaTTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAag                                                >  1:325443/1‑140 (MQ=255)
                                                                                        acaTTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAag                                                >  1:457622/1‑140 (MQ=255)
                                                                                          aTTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGc                                                                                                                                   <  1:1185121/55‑1 (MQ=255)
                                                                                          aTTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGc                                                                                                                                   >  2:1185121/1‑55 (MQ=255)
                                                                                             acacgACTGTTCAGCCTGGCGTACAAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCCGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGAtt                                           <  2:2154652/136‑1 (MQ=255)
                                                                                             aaaGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGAtt                                           <  2:1225926/140‑1 (MQ=255)
                                                                                             aaaGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGAtt                                           <  2:641350/140‑1 (MQ=255)
                                                                                             aaaGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGAtt                                           <  2:236110/140‑1 (MQ=255)
                                                                                                  aCTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATcc                                      <  2:1650519/140‑1 (MQ=255)
                                                                                                     tttCAGCCTGGCTTACTAATCCTTTTAATGAATCTGGCATTGCCAAAAGCATTCCTTTCCGAGTGCTTTCTGGCAATGTCTTTTTTTATGTTTATGACAGCGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCaaag                                 <  1:1874139/140‑4 (MQ=255)
                                                                                                     tttCAGCCTGGCTTACTAATCCTTTTAATGAATCTGGCATTGCCAAAAGCATTCCTTTCCGAGTGCTTTCTGGCAATGTCTTTTTTTATGTTTATGACAGCGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCaaag                                 <  1:1981214/140‑4 (MQ=255)
                                                                                                      ttCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTCACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCaga                                  >  1:662280/1‑140 (MQ=255)
                                                                                                      ttCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCaga                                  >  2:545804/1‑140 (MQ=255)
                                                                                                              ggattACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAAtata                          <  2:1200960/137‑1 (MQ=255)
                                                                                                              ggCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAAtata                          <  2:948770/140‑1 (MQ=255)
                                                                                                                cTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATaaa                        >  1:1646355/1‑140 (MQ=255)
                                                                                                                cTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATaaa                        >  1:2125902/1‑140 (MQ=255)
                                                                                                                      aaTCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTCACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGtaaa                  >  2:976556/1‑140 (MQ=255)
                                                                                                                      aaTCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGtaaa                  >  2:906930/1‑140 (MQ=255)
                                                                                                                            ttttGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGTtaaaataaaa            >  2:713244/1‑140 (MQ=255)
                                                                                                                            ttttGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGtaaaataaaa            >  2:1085865/1‑140 (MQ=255)
                                                                                                                             tttGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGTaaaataaaaa           >  2:1069706/1‑140 (MQ=255)
                                                                                                                               tgtgAACTCGGACATTGCCAATAGTGTTCCTTTCGGGATGCTTGCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCACAGAAGAGATTGATGCCAGAAGAATATAAAGGTaaaataaaaata         >  1:21108/1‑140 (MQ=255)
                                                                                                                               tgtgAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGTaaaataaaaata         >  1:685948/1‑140 (MQ=255)
                                                                                                                                    aCTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGTAAAATAAAAATAAATCa    <  1:2370962/140‑1 (MQ=255)
                                                                                                                                    aCTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGTAAAATAAAAATAAATCa    <  1:1888319/140‑1 (MQ=255)
                                                                                                                                    aCTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGTAAAATAAAAATAAATCa    <  1:1512847/140‑1 (MQ=255)
                                                                                                                                      tCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGTAAAATAAAAATAAATCaaa  <  2:113109/140‑1 (MQ=255)
                                                                                                                                      tCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTGTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGTAAAATAAAAATAAATCaaa  >  2:248963/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                       
GTTGCCATCATTGGTGCAAATTCTACACCGCTTAAAGGTCTTCCTGCTTCCTTGATGAAAGAAGCAAGTAACGTACGCTACTTAACTCACATTAAAGGACTTTTCAGCCTGGCTTACTAATCCTTTTTGTGAACTCGGACATTGCCAAAAGTGTTCCTTTCGGGATGCTTTCCGGCAATGTCTTTTTTTGTTTTTATTGCTAACGTTAACATTTCATTTTCAAAAAAGAGATTGATCCCAGAAGAATATAAAGGTAAAATAAAAATAAATCAAA  >  NZ_CP034943/1268571‑1268844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: