Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,342,854 T→C 5.0% N67N (AAT→AAC hmpA → NO‑inducible flavohemoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,342,8540TC 5.0% 385.7 / 6.6 120N67N (AAT→AAChmpANO‑inducible flavohemoprotein
Reads supporting (aligned to +/- strand):  ref base T (32/80);  new base C (3/3);  total (35/85)
Fisher's exact test for biased strand distribution p-value = 3.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 129 total aligned reads displayed.

AGAAACCATCACTGGCCGTTTTTACGATCTGATGTTTCAAGACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAACATTACCCGATCGTTGGGAAATATTTGCTGATTGCCATTAAAGATGTATTGGGAGATGCTG  >  NZ_CP034943/1342716‑1342993
                                                                                                                                          |                                                                                                                                           
agaAACCATCACTGGCCGTTTTTACGATCTGATGTTTCAAGACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAata                                                                                                                                            >  2:1356419/1‑140 (MQ=255)
agaAACCATCACTGGCCGTTTTTACGATCTGATGTTTCAAGACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAata                                                                                                                                            >  2:1356394/1‑140 (MQ=255)
    aCCATCACTGGCCGTTTTTACGCTCTGATGTTTCAAGACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGa                                                                                                                                        >  1:793259/1‑140 (MQ=255)
    aCCATCACTGGCCGTTTTTACGATCTGATTTTTCAATACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGACGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTCTTGCAGCTGCGGCAAATATAGa                                                                                                                                        >  1:1525956/1‑140 (MQ=255)
    aCCATCACTGGCCGTTTTTACGATCTGATGTTTCAAGACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGa                                                                                                                                        >  1:1091993/1‑140 (MQ=255)
    aCCATCACTGGCCGTTTTTACGATCTGATGTTTCAAGACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGa                                                                                                                                        >  1:1667680/1‑140 (MQ=255)
    aCCATCACTGGCCGTTTTTAAGATCTGATGTTTCAAGAACATCCATAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAAtatata                                                                                                                                        >  1:464554/1‑138 (MQ=255)
    aCCATCACCGGCCGTTTTTACGATCTGATGTTTCAAGACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCGTGCAAATGCCCGTATTGCAGCTGCGGCAAATATAGa                                                                                                                                        >  1:1490846/1‑140 (MQ=255)
     ccATCACTGGCCGTTTTTACGATCTGATGTTTCAAGACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGAc                                                                                                                                       >  1:1327577/1‑140 (MQ=255)
     ccATCACTGGCCGTTTTTACGATCTGATGTTTCAAGACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGAc                                                                                                                                       >  1:683466/1‑140 (MQ=255)
        tCACTGGCCGTTTTTACGATCTGATGTTTCAAGACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAg                                                                                                                                    >  2:1510994/1‑140 (MQ=255)
          aCTGGCCGTTTTTACGATCTGATGTTTCAAGACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCt                                                                                                                                  <  2:89852/140‑1 (MQ=255)
          aCTGGCCGTTTTTACGATCTGATGTTTCAAGACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCt                                                                                                                                  <  2:1032067/140‑1 (MQ=255)
          aCTGGCCGTTTTTACGATCTGATGTTTCAAGACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCGGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCt                                                                                                                                  <  2:203535/140‑1 (MQ=255)
             ggCCGTTTTTACGATCTGATGTTTCAAGACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCgg                                                                                                                               <  2:1877889/140‑1 (MQ=255)
              gCCGTTTTTACGATCGGATGTTTCAAGACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAAAAAACACAGCGAACTGCACTGGCAAATGCCGTTATTGCAGCTGCGGCAAACATTGACCaattagga                                                                                                                              >  2:1601035/1‑133 (MQ=255)
               ccGTTTTTACGATCTGATGTTTCAAGACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAATAAAACTCAGCGCGCTGCCCTTGCAAATGCCGGTATTGCAGCTGCGGCAAATATAGACCAGCTCGGac                                                                                                                             >  1:750879/1‑139 (MQ=255)
                           tCTGATGTTTCAAGACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCGGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCgg                                                                                                                 <  2:2336308/140‑1 (MQ=255)
                                 gTTTCAAGACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCa                                                                                                           <  2:583073/140‑1 (MQ=255)
                                 gTTTCAAGACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCa                                                                                                           <  2:413826/140‑1 (MQ=255)
                                 gTTTCAAGACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCa                                                                                                           <  2:2132802/140‑1 (MQ=255)
                                 gTTTCAAGACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCa                                                                                                           <  2:1854993/140‑1 (MQ=255)
                                     cAAGACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACACAGCGCGCTGCCCTTGCAAAAGCCGTTATTGCAGCTGCGGCAAATATAGACCCGCACGGACCTATCAGTCCGGTCGTCAAAcc                                                                                                       >  2:2392758/1‑139 (MQ=255)
                                       aGACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGa                                                                                                     >  2:46509/1‑140 (MQ=255)
                                        gACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGac                                                                                                    <  1:889652/140‑2 (MQ=255)
                                        gACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGAt                                                                                                    <  2:1685266/140‑1 (MQ=255)
                                        gACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGAt                                                                                                    <  1:685385/140‑1 (MQ=255)
                                        gACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGAt                                                                                                    <  1:1356419/140‑1 (MQ=255)
                                        gACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGAt                                                                                                    <  2:1492962/140‑1 (MQ=255)
                                        gACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGAt                                                                                                    <  1:2395767/140‑1 (MQ=255)
                                        gACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGAt                                                                                                    <  1:667000/140‑1 (MQ=255)
                                        gACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGAt                                                                                                    <  1:439385/140‑1 (MQ=255)
                                        gACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGAt                                                                                                    <  1:4596/140‑1 (MQ=255)
                                        gACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGAt                                                                                                    <  2:1002093/140‑1 (MQ=255)
                                        gACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAAATATCATTGCGGTCGTCAAACAGAt                                                                                                    <  1:2331309/140‑1 (MQ=255)
                                        gACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCGGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGAt                                                                                                    <  1:1342316/140‑1 (MQ=255)
                                            aTCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGc                                                                                                >  2:867/1‑140 (MQ=255)
                                               cAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCAt                                                                                             <  1:1146748/140‑1 (MQ=255)
                                                  agCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAg                                                                                          <  2:1327577/140‑1 (MQ=255)
                                                  agCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAg                                                                                          <  2:683466/140‑1 (MQ=255)
                                                    cTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCa                                                                                        >  1:157620/1‑140 (MQ=255)
                                                     ttttGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCAc                                                                                       >  2:324242/1‑140 (MQ=255)
                                                      tttGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCAcc                                                                                      >  2:1740033/1‑140 (MQ=255)
                                                       ttGAACATTTTTAATCAAACAAACCAACAGAAGAACACTCAGCGCGCGGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGACCTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCg                                                                                     <  2:330512/140‑1 (MQ=255)
                                                       ttGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCg                                                                                     >  1:1511131/1‑140 (MQ=255)
                                                       ttGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCg                                                                                     >  2:289662/1‑140 (MQ=255)
                                                       ttGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCg                                                                                     >  1:967472/1‑140 (MQ=255)
                                                       ttGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCg                                                                                     <  2:1736133/140‑1 (MQ=255)
                                                       ttGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCg                                                                                     <  2:603422/140‑1 (MQ=255)
                                                       ttGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCGTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCg                                                                                     <  2:2093828/140‑1 (MQ=255)
                                                              tttttAATCAAACACACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATc                                                                              <  1:1456686/140‑1 (MQ=255)
                                                              tttttAATCAAACAAACCAAAAGAAGAAACCTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATc                                                                              <  1:1017167/140‑1 (MQ=255)
                                                              tttttAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACCGATCGGCCATAAGCACCGCAGTATc                                                                              <  1:1437169/140‑1 (MQ=255)
                                                              tttttAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATc                                                                              <  1:24557/140‑1 (MQ=255)
                                                              tttttAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATc                                                                              <  1:2054974/140‑1 (MQ=255)
                                                              tttttAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATc                                                                              <  1:2065391/140‑1 (MQ=255)
                                                              tttttAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATc                                                                              <  1:649582/140‑1 (MQ=255)
                                                              tttttAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATc                                                                              <  1:2244829/140‑1 (MQ=255)
                                                              tttttAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATc                                                                              <  2:157620/140‑1 (MQ=255)
                                                              tttttAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATc                                                                              <  1:2366224/140‑1 (MQ=255)
                                                              tttttAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCGGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATc                                                                              <  1:1902135/140‑1 (MQ=255)
                                                                 ttAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCAGAAGCACCGCAGTATCGGa                                                                           >  2:584863/1‑140 (MQ=255)
                                                                    aTCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAAtt                                                                        <  1:1997349/140‑1 (MQ=255)
                                                                    aTCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAAGGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAAtt                                                                        <  2:1248972/140‑1 (MQ=255)
                                                                         acaaacCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGc                                                                   >  1:1902837/1‑140 (MQ=255)
                                                                          caaacCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCt                                                                  <  2:1091993/140‑1 (MQ=255)
                                                                          caaacCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCt                                                                  <  2:793259/140‑1 (MQ=255)
                                                                          caaacCAAAAGAAGAAAACTCAGCGCGCGGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCt                                                                  <  2:1667680/140‑1 (MQ=255)
                                                                             acCAAAAGAAGAAAACTCAGCGCGCTGCCCGTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGaa                                                               <  1:1580104/140‑1 (MQ=255)
                                                                             acCAAAAGAAAAAAACACAGCGAACTGCACTGGCAAATGCCGTTATTGCAGCTGCGGCAAACATTGACCAATTAGGAAACATCATTCCGGTTGTCAAACAAATCGGCCACAAGCACCGCAGTATCGGAATTAAGCCTGag                                                               <  1:1601035/140‑2 (MQ=255)
                                                                             acCAAAAGAAAAAAACACAGCGAACTGCACTGGCAAATGCCGTTATTGCAGCTGCGGCAAACATTGACCAATTAGGAAACATCATTCCGGTTGTCAAACAAATCGGCCACAAGCACCGCAGTATCGGAATTAAGCCTGag                                                               <  1:1824331/140‑2 (MQ=255)
                                                                             acCAAAAGAAAAAAACACAGCGAACTGCACTGGCAAATGCCGTTATTGCAGCTGCGGCAAACATTGACCAATTAGGAAACATCATTCCGGTTGTCAAACAAATCGGCCACAAGCACCGCAGTATCGGAATTAAGCCTGag                                                               <  1:593025/140‑2 (MQ=255)
                                                                                   agaagaAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAACATTAc                                                         >  1:2288815/1‑140 (MQ=255)
                                                                                                 cgcgcTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAACATTACCCGATCGTTGGGaa                                           >  2:2244226/1‑140 (MQ=255)
                                                                                                      tGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAACATTACCCGATCGTTGGGAAATAtt                                      >  2:642576/1‑140 (MQ=255)
                                                                                                             gCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAACATTACCCGATCGTTGGGAAATATTTGCTGAt                               <  1:76787/140‑1 (MQ=255)
                                                                                                             gCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAACATTACCCGATCGTTGGGAAATATTTGCTGAt                               <  1:752433/140‑1 (MQ=255)
                                                                                                             gCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAACATTACCCGATCGTTGGGAAATATTTGCTGAt                               <  1:139774/140‑1 (MQ=255)
                                                                                                             gCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAACATTACCCGATCGTTGGGAAATATTTGCTGAt                               <  1:2087338/140‑1 (MQ=255)
                                                                                                               aaaTGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAACATTACCCGATCGTTGGGAAATATTTGCTGATTg                             <  2:1687310/140‑1 (MQ=255)
                                                                                                               aaaTGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAACATTACCCGATCGTTGGGAAATATTTGCTGATTg                             <  2:1041745/140‑1 (MQ=255)
                                                                                                                aaTGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAACATTACCCGATCGTTGGGAAATATTTGCTGATTGc                            <  2:1834090/140‑1 (MQ=255)
                                                                                                                aaTGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAACATTACCCGATCGTTGGGAAATATTTGCTGATTGc                            <  2:1825158/140‑1 (MQ=255)
                                                                                                                  tGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAACATTACCCGATCGTTGGGAAATATTTGCTGATTGCCa                          >  2:2132740/1‑140 (MQ=255)
                                                                                                                   gCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAACATTACCCGATCGTTGGGAAATATTTGCTGATTGCCAt                         <  1:1281447/140‑1 (MQ=255)
                                                                                                                   gCCGTTATTGCAGCTGAGGCAAATATAGACCACCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCTCAGTATCGGAATTAAGGCTGAACATTACCCGATCGTTGGGAAATATTTGCTGATTGCCAt                         <  1:1212977/140‑1 (MQ=255)
                                                                                                                     cGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAACATTACCCGATCGTTGGGAAATATTTGCTGATTGCCATTa                       <  2:577021/140‑1 (MQ=255)
                                                                                                                       ttATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAACATTACCCGATCGTTGGGAAATATTTGCTGATTGCCATTaaa                     <  2:796572/140‑1 (MQ=255)
                                                                                                                        tATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAACATTACCCGATCGTTGGGAAATATTTGCTGATTGCCATTAAAg                    <  2:1511131/140‑1 (MQ=255)
                                                                                                                          ttGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAACATTACCCGATCGTTGGGAAATATTTGCTGATTGCCATTAAAGAt                  <  2:1771000/140‑1 (MQ=255)
                                                                                                                             cAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAACATTACCCGATCGTTGGGAAATATTTGCTGATTGCCATTAAAGATGTa               <  2:625375/140‑1 (MQ=255)
                                                                                                                             cAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAACATTACCCGATCGTTGGGAAATATTTGCTGATTGCCATTAAAGATGTa               <  2:1969176/140‑1 (MQ=255)
                                                                                                                             cAGCTGCGGCAAAGATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAACATTACCCGATCGTTGGGAAATATTTGCTGATTGCCATTAAAGATGTa               <  2:624977/140‑1 (MQ=255)
                                                                                                                               gCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAACATTACCCGATCGTTGGGAAATATTTGCTGATTGCCATTAAAGATGTAtt             >  1:2250544/1‑140 (MQ=255)
                                                                                                                                    ggCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAACATTACCCGATCGTTGGGAAATATTTGCTGATTGCCATTAAAGATGTATTGGgag        <  2:878065/140‑1 (MQ=255)
                                                                                                                                    ggCAAAGATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAAGATGACCCGATCGTTGGGAAATATTTGCTGATTGCCATTAAAGATGTATTGGgag        <  2:1570673/140‑1 (MQ=255)
                                                                                                                                          tataGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAACATTACCCGATCGTTGGGAAATATTTGCTGATTGCCATTAAAGATGTATTGGGAGAtgctg  <  2:144713/140‑1 (MQ=255)
                                                                                                                                          tataGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAACATTACCCGATCGTTGGGAAATATTTGCTGATTGCCATTAAAGATGTATTGGGAGAtgctg  <  2:1256801/140‑1 (MQ=255)
                                                                                                                                          tataGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAACATTACCCGATCGTTGGGAAATATTTGCTGATTGCCATTAAAGATGTATTGGGAGAtgctg  <  2:2188761/140‑1 (MQ=255)
                                                                                                                                          tataGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAACATTACCCGATCGTTGGGAAATATTTGCTGATTGCCATTAAAGAGGTATTGGGAGAtgctg  <  2:1610684/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
AGAAACCATCACTGGCCGTTTTTACGATCTGATGTTTCAAGACCATCCAGAGCTTTTGAACATTTTTAATCAAACAAACCAAAAGAAGAAAACTCAGCGCGCTGCCCTTGCAAATGCCGTTATTGCAGCTGCGGCAAATATAGACCAGCTCGGAACTATCATTCCGGTCGTCAAACAGATCGGCCATAAGCACCGCAGTATCGGAATTAAGGCTGAACATTACCCGATCGTTGGGAAATATTTGCTGATTGCCATTAAAGATGTATTGGGAGATGCTG  >  NZ_CP034943/1342716‑1342993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: