Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,365,757 G→A 5.0% S85S (TCG→TCA EO946_RS07135 → PepSY domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,365,7570GA 5.0% 323.7 / 3.6 99S85S (TCG→TCAEO946_RS07135PepSY domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (32/61);  new base A (3/2);  total (36/63)
Fisher's exact test for biased strand distribution p-value = 3.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAGAAGCCTCTTATGAAGGAAAGGTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTGCCACATATACCATTCAA  >  NZ_CP034943/1365623‑1365889
                                                                                                                                      |                                                                                                                                    
gTAGAAGCCTCTTATGAAGGAAAGGTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGaa                                                                                                                                 <  2:1354650/140‑1 (MQ=255)
gTAGAAGCCTCTTATGAAGGAAAGGTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGaa                                                                                                                                 <  2:1549287/140‑1 (MQ=255)
gTAGAAGCCTCTTATGAAGGAAAGGTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGaa                                                                                                                                 <  1:1195447/140‑1 (MQ=255)
gTAGAAGCCTCTTATGAAGGAAAGGTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGaa                                                                                                                                 <  2:2017788/140‑1 (MQ=255)
gTAGAAGCCTCTTATGAAGGAAAGGTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGaa                                                                                                                                 <  2:793047/140‑1 (MQ=255)
gTAGAAGCCTCTTATGAAGGAAAGGTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGaa                                                                                                                                 <  1:2214708/140‑1 (MQ=255)
gTAGAAGCCTCTTATGAAGGAAAGGTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGaa                                                                                                                                 <  1:1634324/140‑1 (MQ=255)
  aGAAGCCTCTTATGAAGGAAAGGTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACa                                                                                                                               <  2:325104/140‑1 (MQ=255)
                  ggAAAGGTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGaa                                                                                                               >  1:2428146/1‑140 (MQ=255)
                  ggAAAGGTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGaa                                                                                                               >  1:266964/1‑140 (MQ=255)
                     aaGGTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATcc                                                                                                            >  2:841445/1‑140 (MQ=255)
                     aaGGTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATcc                                                                                                            >  1:870942/1‑140 (MQ=255)
                      aGGTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTCAAAGCGATGAATCCa                                                                                                           <  1:2072588/140‑1 (MQ=255)
                      aGGTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCa                                                                                                           <  1:2072550/140‑1 (MQ=255)
                      aGGTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCa                                                                                                           <  1:797976/140‑1 (MQ=255)
                        gTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCACACTCAGCTAACATTCTTTCGATGAACAGAGTCAAAGCGAGGAATCCATc                                                                                                         >  2:1871338/1‑140 (MQ=255)
                        gTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATc                                                                                                         <  1:2124567/140‑1 (MQ=255)
                        gTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATc                                                                                                         <  1:606169/140‑1 (MQ=255)
                        gTCACGAAACTAACACAGTCAAAGTATAAAAAAACATATGACATGACCCTTGAAAATCCAACGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATc                                                                                                         <  1:1952488/140‑1 (MQ=255)
                             gAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCa                                                                                                    >  1:924237/1‑140 (MQ=255)
                             gAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCa                                                                                                    >  1:924249/1‑140 (MQ=255)
                             gAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCa                                                                                                    >  1:1976355/1‑140 (MQ=255)
                              aaaGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCAt                                                                                                   <  2:292756/140‑1 (MQ=255)
                              aaaGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCAt                                                                                                   <  2:2368823/140‑1 (MQ=255)
                              aaaGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCAt                                                                                                   <  2:2332934/140‑1 (MQ=255)
                                 gcaaCACAATCAAAGGATAAAAAAACATATGATATAACCCTTGAAAATCCAAAGGGAACTTACTTTGTAAAAGCAGACGCAATATCGGCTGACATTCTTTCAATGAATAGAGTAAAAGCGATGAATCCATCTGCCATGAc                                                                                                >  1:188033/3‑140 (MQ=255)
                                 gcaaCACAATCAAAGGATAAAAAAACATATGATATAACCCTTGAAAATCCAAAGGGAACTTACTTTGTAAAAGCAGACGCAATATCGGCTGACATTCTTTCAATGAATAGAGTAAAAGCGATGAATCCATCTGCCATGAc                                                                                                >  1:1409462/3‑140 (MQ=255)
                                  tAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACa                                                                                               >  2:2251873/1‑140 (MQ=255)
                                  tAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACa                                                                                               >  2:1357668/1‑140 (MQ=255)
                                  tAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACa                                                                                               <  1:782492/140‑1 (MQ=255)
                                  tAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACa                                                                                               <  1:2153211/140‑1 (MQ=255)
                                  tAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACa                                                                                               <  1:1837027/140‑1 (MQ=255)
                                         gTCAAAGGATAAAAAAACATATGACATGACCCTTGACAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAaag                                                                                        >  1:1190762/1‑140 (MQ=255)
                                         gTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAaag                                                                                        >  1:872954/1‑140 (MQ=255)
                                          tCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAaaga                                                                                       >  1:1516984/1‑140 (MQ=255)
                                                 ctAAAAAAACAAATGACATGACCCTTGAAAATCCAAATGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAg                                                                                <  2:351993/139‑1 (MQ=255)
                                                 aTAACAAAACATCTGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAg                                                                                <  2:1825944/140‑1 (MQ=255)
                                                 aTAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAg                                                                                <  1:183084/140‑1 (MQ=255)
                                                    aaaaaaCATATGACATTAAAATATAAAATCCAAAGGGAACTTATTTTATAAAATATTACGCAAAATCAGCTAACATTCTTTCGATGAACAGAATAAAACCGATGAATCCATCTGCCATGAAAGAAAAAAAAAcccaacaa                                                                             >  2:1412272/1‑134 (MQ=255)
                                                    aaaaaaCATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCAt                                                                             >  2:1727414/1‑140 (MQ=255)
                                                    aaaaaaCATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCAt                                                                             >  2:225913/1‑140 (MQ=255)
                                                    aaaaaaCATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCAt                                                                             >  2:1399774/1‑140 (MQ=255)
                                                    aaaaaaCATATAACATTAAAATTGAAAATCCAAAGGGAAATTATAAAATAAAATCTGACGCAAAATCAGATAAAATTCTTTCTATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCAt                                                                             >  2:1122767/1‑140 (MQ=255)
                                                      aaaaCATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCAtct                                                                           <  2:1231823/140‑1 (MQ=255)
                                                                 catgacCCTGGAAAATCCAAAGGGAACGTATTTTATAACAGCTGACGCAAAATCAGCTAACATTCGTTCGATGAACAGAGTAAAAGCGATGCATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGc                                                                <  2:794211/140‑1 (MQ=255)
                                                                   tgacCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCgg                                                              <  1:1727414/140‑1 (MQ=255)
                                                                   tgacCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCgg                                                              <  1:872669/140‑1 (MQ=255)
                                                                   tgacCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCgg                                                              <  1:1399774/140‑1 (MQ=255)
                                                                   tgacCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCgg                                                              <  1:225913/140‑1 (MQ=255)
                                                                   taacCCTTGAAAATCCAAAGGGAACTTACTTTGTAAAAGCAGACGCAATATCGGCTGACATTCTTTCAATGAATAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCgg                                                              <  2:188033/138‑1 (MQ=255)
                                                                   taacCCTTGAAAATCCAAAGGGAACTTACTTTGTAAAAGCAGACGCAATATCGGCTGACATTCTTTCAATGAATAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCgg                                                              <  2:1409462/138‑1 (MQ=255)
                                                                    gacCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCggg                                                             >  1:1726816/1‑140 (MQ=255)
                                                                     acCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGt                                                            >  1:877511/1‑140 (MQ=255)
                                                                     acCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGt                                                            >  1:513795/1‑140 (MQ=255)
                                                                       ccTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGc                                                          >  2:620939/1‑140 (MQ=255)
                                                                         ttGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCg                                                        >  1:246580/1‑140 (MQ=255)
                                                                          tGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCgg                                                       >  1:1311607/1‑140 (MQ=255)
                                                                                          aCTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAACAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAaaaca                                       <  1:1166342/140‑1 (MQ=255)
                                                                                           cTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAaaacaa                                      >  1:1386231/1‑140 (MQ=255)
                                                                                            ttATTTTATAAAAGCTGACGCAACATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAaaacaaa                                     <  2:1516725/140‑1 (MQ=255)
                                                                                            ttATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAaaacaaa                                     <  2:112910/140‑1 (MQ=255)
                                                                                            ttATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAaaacaaa                                     <  2:829386/140‑1 (MQ=255)
                                                                                            ttATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAaaacaaa                                     <  2:1515451/140‑1 (MQ=255)
                                                                                            ttATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAaaacaaa                                     <  2:112885/140‑1 (MQ=255)
                                                                                            ttATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAaaacaaa                                     <  2:1118751/140‑1 (MQ=255)
                                                                                            ttATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAaaacaaa                                     <  2:13648/140‑1 (MQ=255)
                                                                                            ttATGTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAaaacaaa                                     <  2:1714521/140‑1 (MQ=255)
                                                                                              aTTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAaacaaaca                                   <  2:870942/140‑1 (MQ=255)
                                                                                                 ttATAAAATATGACTCATAATGTGCTTACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCTTATGCCATGACAGAATAAGAAGCGGAGCATCTGGCGGTTGCGCGGGTGCCTGGAACCGTGAAAAAACAAACagaa                                <  2:1858794/140‑1 (MQ=255)
                                                                                                 ttATAAAAGCTGAAGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCTAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACacaa                                <  1:888648/140‑1 (MQ=255)
                                                                                                   ataAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCTGGTGCCGGGAACCGTCAAAAAACAAACacaaca                              >  2:941125/1‑140 (MQ=255)
                                                                                                   ataAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACacaaca                              >  2:2403863/1‑140 (MQ=255)
                                                                                                   ataAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACacaaca                              >  1:386331/1‑140 (MQ=255)
                                                                                                   ataAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACacaaca                              >  1:1691981/1‑140 (MQ=255)
                                                                                                      aaaGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGaa                           <  1:1357668/140‑1 (MQ=255)
                                                                                                             aCGCAAAATCAGCTAACATTCTTTCGATGAACAGATTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGtt                    <  1:2230616/140‑1 (MQ=255)
                                                                                                             aCGCAAAATCAGCTAACATTCTTTCGATGAACAGATTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGtt                    <  2:2432301/140‑1 (MQ=255)
                                                                                                             aCGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGtt                    <  2:634775/140‑1 (MQ=255)
                                                                                                             aCGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGtt                    <  2:978262/140‑1 (MQ=255)
                                                                                                             aCGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGtt                    <  2:262816/140‑1 (MQ=255)
                                                                                                             aCGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGtt                    <  2:2432135/140‑1 (MQ=255)
                                                                                                             aCGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGtt                    <  2:1342552/140‑1 (MQ=255)
                                                                                                             aCGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGtt                    <  2:2223725/140‑1 (MQ=255)
                                                                                                             aCGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGtt                    <  2:2216782/140‑1 (MQ=255)
                                                                                                             aCGCAAAATCAGCTAACATTCTGTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGtt                    <  2:24759/140‑1 (MQ=255)
                                                                                                              cGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAACCACAACAGAATCGTGTTg                   <  1:2315747/140‑1 (MQ=255)
                                                                                                              cGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTg                   <  1:883919/140‑1 (MQ=255)
                                                                                                              cGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTg                   <  1:660011/140‑1 (MQ=255)
                                                                                                              cGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTg                   <  1:599802/140‑1 (MQ=255)
                                                                                                              cGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTg                   <  1:599813/140‑1 (MQ=255)
                                                                                                               gCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTGc                  <  2:1367351/140‑1 (MQ=255)
                                                                                                               gCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTGc                  <  1:841445/140‑1 (MQ=255)
                                                                                                                cAAAATGAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTGcc                 <  2:2119688/140‑1 (MQ=255)
                                                                                                                cAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTGcc                 <  1:1920860/140‑1 (MQ=255)
                                                                                                                       ggCTGACATTCTTTCAATGAATAGAGTAAAAGCAGTGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACGGGCAAAAAACAAACACGGCAAAGTCAAGTTGCCACTtata          >  1:2268561/2‑140 (MQ=255)
                                                                                                                           aaCATTCTTTCGATGACCAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCGTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTGCCACATATCCCAt      >  1:945731/1‑140 (MQ=255)
                                                                                                                           aaCATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTGCCACATATACCAt      >  1:507114/1‑140 (MQ=255)
                                                                                                                           aaCATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTGCCACATATACCAt      >  1:364727/1‑140 (MQ=255)
                                                                                                                               ttCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTGCCACATATACCATTCaa  >  1:1008105/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                    
GTAGAAGCCTCTTATGAAGGAAAGGTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTGCCACATATACCATTCAA  >  NZ_CP034943/1365623‑1365889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: