Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,365,763 C→T 5.2% N87N (AAC→AAT EO946_RS07135 → PepSY domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,365,7630CT 5.2% 310.5 / 4.5 96N87N (AAC→AATEO946_RS07135PepSY domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (34/57);  new base T (3/2);  total (37/59)
Fisher's exact test for biased strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

AGAAGCCTCTTATGAAGGAAAGGTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTGCCACATATACCATTCAAAAAGAAGATG  >  NZ_CP034943/1365625‑1365899
                                                                                                                                          |                                                                                                                                        
aGAAGCCTCTTATGAAGGAAAGGTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACa                                                                                                                                         <  2:325104/140‑1 (MQ=255)
                ggAAAGGTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGaa                                                                                                                         >  1:2428146/1‑140 (MQ=255)
                ggAAAGGTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGaa                                                                                                                         >  1:266964/1‑140 (MQ=255)
                   aaGGTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATcc                                                                                                                      >  2:841445/1‑140 (MQ=255)
                   aaGGTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATcc                                                                                                                      >  1:870942/1‑140 (MQ=255)
                    aGGTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTCAAAGCGATGAATCCa                                                                                                                     <  1:2072588/140‑1 (MQ=255)
                    aGGTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCa                                                                                                                     <  1:797976/140‑1 (MQ=255)
                    aGGTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCa                                                                                                                     <  1:2072550/140‑1 (MQ=255)
                      gTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCACACTCAGCTAACATTCTTTCGATGAACAGAGTCAAAGCGAGGAATCCATc                                                                                                                   >  2:1871338/1‑140 (MQ=255)
                      gTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATc                                                                                                                   <  1:2124567/140‑1 (MQ=255)
                      gTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATc                                                                                                                   <  1:606169/140‑1 (MQ=255)
                      gTCACGAAACTAACACAGTCAAAGTATAAAAAAACATATGACATGACCCTTGAAAATCCAACGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATc                                                                                                                   <  1:1952488/140‑1 (MQ=255)
                           gAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCa                                                                                                              >  1:924249/1‑140 (MQ=255)
                           gAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCa                                                                                                              >  1:924237/1‑140 (MQ=255)
                           gAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCa                                                                                                              >  1:1976355/1‑140 (MQ=255)
                            aaaGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCAt                                                                                                             <  2:292756/140‑1 (MQ=255)
                            aaaGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCAt                                                                                                             <  2:2368823/140‑1 (MQ=255)
                            aaaGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCAt                                                                                                             <  2:2332934/140‑1 (MQ=255)
                               gcaaCACAATCAAAGGATAAAAAAACATATGATATAACCCTTGAAAATCCAAAGGGAACTTACTTTGTAAAAGCAGACGCAATATCGGCTGACATTCTTTCAATGAATAGAGTAAAAGCGATGAATCCATCTGCCATGAc                                                                                                          >  1:188033/3‑140 (MQ=255)
                               gcaaCACAATCAAAGGATAAAAAAACATATGATATAACCCTTGAAAATCCAAAGGGAACTTACTTTGTAAAAGCAGACGCAATATCGGCTGACATTCTTTCAATGAATAGAGTAAAAGCGATGAATCCATCTGCCATGAc                                                                                                          >  1:1409462/3‑140 (MQ=255)
                                tAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACa                                                                                                         >  2:1357668/1‑140 (MQ=255)
                                tAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACa                                                                                                         >  2:2251873/1‑140 (MQ=255)
                                tAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACa                                                                                                         <  1:782492/140‑1 (MQ=255)
                                tAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACa                                                                                                         <  1:2153211/140‑1 (MQ=255)
                                tAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACa                                                                                                         <  1:1837027/140‑1 (MQ=255)
                                       gTCAAAGGATAAAAAAACATATGACATGACCCTTGACAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAaag                                                                                                  >  1:1190762/1‑140 (MQ=255)
                                       gTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAaag                                                                                                  >  1:872954/1‑140 (MQ=255)
                                        tCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAaaga                                                                                                 >  1:1516984/1‑140 (MQ=255)
                                               ctAAAAAAACAAATGACATGACCCTTGAAAATCCAAATGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAg                                                                                          <  2:351993/139‑1 (MQ=255)
                                               aTAACAAAACATCTGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAg                                                                                          <  2:1825944/140‑1 (MQ=255)
                                               aTAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAg                                                                                          <  1:183084/140‑1 (MQ=255)
                                                  aaaaaaCATATGACATTAAAATATAAAATCCAAAGGGAACTTATTTTATAAAATATTACGCAAAATCAGCTAACATTCTTTCGATGAACAGAATAAAACCGATGAATCCATCTGCCATGAAAGAAAAAAAAAcccaacaa                                                                                       >  2:1412272/1‑134 (MQ=255)
                                                  aaaaaaCATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCAt                                                                                       >  2:1399774/1‑140 (MQ=255)
                                                  aaaaaaCATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCAt                                                                                       >  2:1727414/1‑140 (MQ=255)
                                                  aaaaaaCATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCAt                                                                                       >  2:225913/1‑140 (MQ=255)
                                                  aaaaaaCATATAACATTAAAATTGAAAATCCAAAGGGAAATTATAAAATAAAATCTGACGCAAAATCAGATAAAATTCTTTCTATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCAt                                                                                       >  2:1122767/1‑140 (MQ=255)
                                                    aaaaCATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCAtct                                                                                     <  2:1231823/140‑1 (MQ=255)
                                                               catgacCCTGGAAAATCCAAAGGGAACGTATTTTATAACAGCTGACGCAAAATCAGCTAACATTCGTTCGATGAACAGAGTAAAAGCGATGCATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGc                                                                          <  2:794211/140‑1 (MQ=255)
                                                                 tgacCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCgg                                                                        <  1:225913/140‑1 (MQ=255)
                                                                 tgacCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCgg                                                                        <  1:872669/140‑1 (MQ=255)
                                                                 tgacCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCgg                                                                        <  1:1399774/140‑1 (MQ=255)
                                                                 tgacCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCgg                                                                        <  1:1727414/140‑1 (MQ=255)
                                                                 taacCCTTGAAAATCCAAAGGGAACTTACTTTGTAAAAGCAGACGCAATATCGGCTGACATTCTTTCAATGAATAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCgg                                                                        <  2:1409462/138‑1 (MQ=255)
                                                                 taacCCTTGAAAATCCAAAGGGAACTTACTTTGTAAAAGCAGACGCAATATCGGCTGACATTCTTTCAATGAATAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCgg                                                                        <  2:188033/138‑1 (MQ=255)
                                                                  gacCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCggg                                                                       >  1:1726816/1‑140 (MQ=255)
                                                                   acCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGt                                                                      >  1:877511/1‑140 (MQ=255)
                                                                   acCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGt                                                                      >  1:513795/1‑140 (MQ=255)
                                                                     ccTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGc                                                                    >  2:620939/1‑140 (MQ=255)
                                                                       ttGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCg                                                                  >  1:246580/1‑140 (MQ=255)
                                                                        tGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCgg                                                                 >  1:1311607/1‑140 (MQ=255)
                                                                                        aCTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAACAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAaaaca                                                 <  1:1166342/140‑1 (MQ=255)
                                                                                         cTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAaaacaa                                                >  1:1386231/1‑140 (MQ=255)
                                                                                          ttATTTTATAAAAGCTGACGCAACATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAaaacaaa                                               <  2:1516725/140‑1 (MQ=255)
                                                                                          ttATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAaaacaaa                                               <  2:1118751/140‑1 (MQ=255)
                                                                                          ttATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAaaacaaa                                               <  2:829386/140‑1 (MQ=255)
                                                                                          ttATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAaaacaaa                                               <  2:112885/140‑1 (MQ=255)
                                                                                          ttATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAaaacaaa                                               <  2:112910/140‑1 (MQ=255)
                                                                                          ttATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAaaacaaa                                               <  2:1515451/140‑1 (MQ=255)
                                                                                          ttATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAaaacaaa                                               <  2:13648/140‑1 (MQ=255)
                                                                                          ttATGTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAaaacaaa                                               <  2:1714521/140‑1 (MQ=255)
                                                                                            aTTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAaacaaaca                                             <  2:870942/140‑1 (MQ=255)
                                                                                               ttATAAAATATGACTCATAATGTGCTTACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCTTATGCCATGACAGAATAAGAAGCGGAGCATCTGGCGGTTGCGCGGGTGCCTGGAACCGTGAAAAAACAAACagaa                                          <  2:1858794/140‑1 (MQ=255)
                                                                                               ttATAAAAGCTGAAGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCTAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACacaa                                          <  1:888648/140‑1 (MQ=255)
                                                                                                 ataAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCTGGTGCCGGGAACCGTCAAAAAACAAACacaaca                                        >  2:941125/1‑140 (MQ=255)
                                                                                                 ataAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACacaaca                                        >  2:2403863/1‑140 (MQ=255)
                                                                                                 ataAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACacaaca                                        >  1:1691981/1‑140 (MQ=255)
                                                                                                 ataAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACacaaca                                        >  1:386331/1‑140 (MQ=255)
                                                                                                    aaaGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGaa                                     <  1:1357668/140‑1 (MQ=255)
                                                                                                           aCGCAAAATCAGCTAACATTCTTTCGATGAACAGATTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGtt                              <  2:2432301/140‑1 (MQ=255)
                                                                                                           aCGCAAAATCAGCTAACATTCTTTCGATGAACAGATTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGtt                              <  1:2230616/140‑1 (MQ=255)
                                                                                                           aCGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGtt                              <  2:2432135/140‑1 (MQ=255)
                                                                                                           aCGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGtt                              <  2:262816/140‑1 (MQ=255)
                                                                                                           aCGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGtt                              <  2:978262/140‑1 (MQ=255)
                                                                                                           aCGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGtt                              <  2:634775/140‑1 (MQ=255)
                                                                                                           aCGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGtt                              <  2:2216782/140‑1 (MQ=255)
                                                                                                           aCGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGtt                              <  2:1342552/140‑1 (MQ=255)
                                                                                                           aCGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGtt                              <  2:2223725/140‑1 (MQ=255)
                                                                                                           aCGCAAAATCAGCTAACATTCTGTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGtt                              <  2:24759/140‑1 (MQ=255)
                                                                                                            cGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAACCACAACAGAATCGTGTTg                             <  1:2315747/140‑1 (MQ=255)
                                                                                                            cGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTg                             <  1:599802/140‑1 (MQ=255)
                                                                                                            cGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTg                             <  1:599813/140‑1 (MQ=255)
                                                                                                            cGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTg                             <  1:883919/140‑1 (MQ=255)
                                                                                                            cGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTg                             <  1:660011/140‑1 (MQ=255)
                                                                                                             gCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTGc                            <  1:841445/140‑1 (MQ=255)
                                                                                                             gCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTGc                            <  2:1367351/140‑1 (MQ=255)
                                                                                                              cAAAATGAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTGcc                           <  2:2119688/140‑1 (MQ=255)
                                                                                                              cAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTGcc                           <  1:1920860/140‑1 (MQ=255)
                                                                                                                     ggCTGACATTCTTTCAATGAATAGAGTAAAAGCAGTGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACGGGCAAAAAACAAACACGGCAAAGTCAAGTTGCCACTtata                    >  1:2268561/2‑140 (MQ=255)
                                                                                                                         aaCATTCTTTCGATGACCAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCGTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTGCCACATATCCCAt                >  1:945731/1‑140 (MQ=255)
                                                                                                                         aaCATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTGCCACATATACCAt                >  1:507114/1‑140 (MQ=255)
                                                                                                                         aaCATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTGCCACATATACCAt                >  1:364727/1‑140 (MQ=255)
                                                                                                                             ttCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTGCCACATATACCATTCaa            >  1:1008105/1‑140 (MQ=255)
                                                                                                                                      tGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTGCCACATATACCATTCAAAAAGAAGAt   >  2:964541/1‑140 (MQ=255)
                                                                                                                                       gAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTGCCACATATACCATTCAAAAAGAAGATg  <  2:877511/140‑1 (MQ=255)
                                                                                                                                       gAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTGCCACATAGACCATTCAAAAAGAAGATg  <  2:513795/140‑1 (MQ=255)
                                                                                                                                       gAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTGCCACATAGACCATTCAAAAAGAAGATg  <  2:1221110/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                        
AGAAGCCTCTTATGAAGGAAAGGTCACGAAAGTAACACAGTCAAAGGATAAAAAAACATATGACATGACCCTTGAAAATCCAAAGGGAACTTATTTTATAAAAGCTGACGCAAAATCAGCTAACATTCTTTCGATGAACAGAGTAAAAGCGATGAATCCATCTGCCATGACAGAAAAAGAAGCCGAGCATCTCGCGCTTGAGCGGGTGCCGGGAACCGTCAAAAAACAAACACAACAGAATCGTGTTGCCACATATACCATTCAAAAAGAAGATG  >  NZ_CP034943/1365625‑1365899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: