Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,397,487 T→C 5.1% V58V (GTT→GTC mtnE → methionine‑glutamine aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,397,4870TC 5.1% 313.6 / 5.0 97V58V (GTT→GTCmtnEmethionine‑glutamine aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (36/56);  new base C (2/3);  total (38/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

TAAACAATTCTTCGCTTCTTTGGTGCAGAAAGTGAACCGAAAGCTCTCAGAAGGACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGAAGTCGCTGTATTATTCGGCGG  >  NZ_CP034943/1397352‑1397621
                                                                                                                                       |                                                                                                                                      
tAACCAAGTCGTCGCGTCTTTGGTGCAGAAAGTGCACCGAAAGCTCTCAGAAGGACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTg                                                                                                                                    <  2:995188/140‑1 (MQ=255)
tAAACAATTCTTCGCTTCTTTGGTGCAGAAAGTGAACCGAAAGCTCTCAGAAGGACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTg                                                                                                                                    <  2:2090534/140‑1 (MQ=255)
      aTTCTTCGCTTCTTTGGTGCAGAAAGTGAACCGAAAGCTCTCAGAAGGACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTg                                                                                                                              >  1:451400/1‑140 (MQ=255)
      aTTCTTCGCTTCTTTGGTGCAGAAAGTGAACCGAAAGCTCTCAGAAGGACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTg                                                                                                                              <  2:1534433/140‑1 (MQ=255)
       ttcttcGCTTCTTTGGTGCAGAAAGTGAACCGAAAGCTCTCAGAAGGACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGa                                                                                                                             <  1:1137380/140‑1 (MQ=255)
        tcttcGCTTCTTTGGTGCAGAAAGTGAACCGAAAGCTCTCAGAAGGACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGaa                                                                                                                            <  2:2128102/140‑1 (MQ=255)
        tcttcGCTTCTTTGGTGCAGAAAGTGAACCGAAAGCTCTCAGAAGGACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGaa                                                                                                                            <  2:2401839/140‑1 (MQ=255)
        tcttcGCTTCTTTGGTGCAGAAAGTGAACCGAAAGCTCTCAGAAGGACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGaa                                                                                                                            <  2:302096/140‑1 (MQ=255)
        tcttcGCTTCTTTGGTGCAGAAAGTGAACCGAAAGCTCTCAGAAGGACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGaa                                                                                                                            >  2:423482/1‑140 (MQ=255)
        tcttcGCTTCTTTGGTGCAGAAAGTGAACCGAAAGCTCTCAGAAGGACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTCaa                                                                                                                            >  2:1851225/1‑140 (MQ=255)
                 cttTGGTGCAGAAAGTGAACCGAAAGCTCTCAGAAGGACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATaaa                                                                                                                   >  1:996375/1‑140 (MQ=255)
                  tttGGTGCAGAAAGTGAACCGAAAGCTCTCAGAAGGACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAACTGAAACGAGCCGTTGCTGATCCTGAAAATCCTaact                                                                                                                  >  2:1590252/1‑138 (MQ=255)
                  tttGGTGCAGAAAGTGAACCGAAAGCTCTCAGAAGGACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAat                                                                                                                  >  2:363519/1‑140 (MQ=255)
                  tttGGTGCAGAAAGTGAACCGAAAGCTCTCAGAAGGACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATAATAAat                                                                                                                  >  2:2180660/1‑140 (MQ=255)
                     ggTGCAGAAAGTGAACCGAAAGCTCTCAGAAGGACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATAtt                                                                                                               <  2:1743629/140‑1 (MQ=255)
                           gAAAGTGAACCGAAAGCTCTCAGAAGGACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGACCATATCGTCGAGGAAATGAACCGAGCCGTTGCTGATCCTGACAATCATAAATATTCGTCtt                                                                                                         >  1:239081/1‑140 (MQ=255)
                              aGTGAACCGAAAGCTCTCAGAAGGACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTc                                                                                                      <  2:276111/140‑1 (MQ=255)
                              aGTGAACCGAAAGCTCTCAGAAGGACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTc                                                                                                      <  2:1053080/140‑1 (MQ=255)
                              aGTGAACCGAAAGCTCTCAGAAGGACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTc                                                                                                      <  2:1426444/140‑1 (MQ=255)
                              aGTGAACCGAAAGCTCTCAGAAGGACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTc                                                                                                      <  2:1737907/140‑1 (MQ=255)
                              aGTGAACCGAAAGCTCTCAGAAGGACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTc                                                                                                      <  1:1790889/140‑1 (MQ=255)
                              aGTGAACCGAAAGCTCTCAGAAGGACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTc                                                                                                      <  2:2377835/140‑1 (MQ=255)
                                  aaCCGAAAGCTTGCAGAAGGACATGACGTTATCAATCTGGGACAGGGAAATCCAGATCAGCCGACTCCTGAGCATATTGTTGAGGAGATGAAACGAGCTGTCGCTGATCCTGAGAATCATAAATATTCGTCTTTTCGCgg                                                                                                  <  1:1481665/140‑1 (MQ=255)
                                  aaCCGAAAGCTCTCAGAAGGACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCgg                                                                                                  <  1:2332951/140‑1 (MQ=255)
                                  aaCCGAAAGCTCTCAGAAGGACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCgg                                                                                                  <  1:2429809/140‑1 (MQ=255)
                                  aaCCGAAAGCTCTCAGAAGGACACGACGTTATCAATCTCAGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCgg                                                                                                  <  1:2261912/140‑1 (MQ=255)
                                         aGCTCTCAGAAGGACACGACGTTATAAATCTCGGACAGGGAAATCCGGATCAGCAGACTCCGGAACATATCGTCGAGGAAATCAAAAGAGCCGTTGCTTATCCTGAAAATCATAAATATTCGTCTTTTCGCGTCTCATAc                                                                                           <  2:182439/140‑1 (MQ=255)
                                            tctcAGACGGACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGACCATCTCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATACATATTCGTCTTTTCGCGGCTCATACAGc                                                                                        <  2:331700/140‑1 (MQ=255)
                                            cctcAGCAGGACACGACCTGATCAATCCCGCACACGGACCTCCGGACCAGCCGACTCCCGAAGAGATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCCGCAAATCCGACATATTCGTCTGTTCGCGGCTCATACAGc                                                                                        <  2:1915650/139‑1 (MQ=255)
                                                  aaGGCCACGACGTTATCAATCTCGCACAGGGAAATCCGTATCAGCCGACTCCGGAACATATCGTGTTGGAAATGAAACTCGCCGTTGCTGCTCCTGAAAATCATAAATATTCGACTTTTCGCGCCTCATACACCCTGaaa                                                                                  <  1:567379/140‑1 (MQ=255)
                                                  aaGGACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGaaa                                                                                  <  1:1003149/140‑1 (MQ=255)
                                                     gACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGACATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCa                                                                               >  1:586984/1‑140 (MQ=255)
                                                     gACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCa                                                                               >  1:1344644/1‑140 (MQ=255)
                                                     gACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCa                                                                               >  1:799011/1‑140 (MQ=255)
                                                        acgacgTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGcc                                                                            >  1:1032893/1‑140 (MQ=255)
                                                        acgacgTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGcc                                                                            >  1:255797/1‑140 (MQ=255)
                                                         cgacgTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCg                                                                           <  2:1818403/140‑1 (MQ=255)
                                                         cgacgTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCg                                                                           <  2:1683428/140‑1 (MQ=255)
                                                         cgacgTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACAGATCGTCGAGGAAATGCAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCg                                                                           <  2:1403189/140‑1 (MQ=255)
                                                                 tCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCgcagcagca                                                                   <  2:76434/140‑1 (MQ=255)
                                                                 tCAATCTCGGACAGCGACATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATACATATTCGTCTTTTCGCGGCTCAGACAGCCTGAAATCAGCCgcagcagca                                                                   <  1:1965074/140‑1 (MQ=255)
                                                                        cGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGATCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGACGCAGCAGCATTTtata                                                            >  1:1582626/1‑140 (MQ=255)
                                                                        cGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTtata                                                            >  1:2363553/1‑140 (MQ=255)
                                                                        cGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTtata                                                            >  1:1890920/1‑140 (MQ=255)
                                                                          gACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAg                                                          >  1:495549/1‑140 (MQ=255)
                                                                             aGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCga                                                       <  2:1344644/140‑1 (MQ=255)
                                                                              gggAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTTCTGATCCTTAAAATCATAAATATTAGTCTTTTAGCGGCTCATACAGCCTGAAATAAGCCCCAGCAGCATTTTATAAGCgag                                                      >  1:23807/1‑140 (MQ=255)
                                                                              gggAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCTCGGCTCAAACAGACTGAAAACAGCCGCAGAAGCATTTTATAAACgag                                                      >  1:2426310/1‑140 (MQ=255)
                                                                               ggAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCgaga                                                     >  1:1685273/1‑140 (MQ=255)
                                                                               ggAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCgaga                                                     >  1:1685333/1‑140 (MQ=255)
                                                                               ggAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCgaga                                                     >  1:2081509/1‑140 (MQ=255)
                                                                               ggAAATCCAGATCAGCCGACTCCTGAGCATATTGTTGAGGAGATGAAACGAGCTGTCGCTGATCCTGAGAATCATAAATATTCGTCTTTTCGCGGCTCATACCGTCTGAAGTCAGCGGCTGCTGCATTTTACAAAAgaga                                                     >  2:1844741/1‑140 (MQ=255)
                                                                               ggAAATCCAGATCAGCCGACTCCTGAGCATATTGTTGAGGAGATGAAACGAGCTGTCGCTGATCCTGAGAATCATAAATATTCGTCTTTTCGCGGCTCATACCGTCTGAAGTCAGCGGCTGCTGCATTTTACAAAAgaga                                                     >  2:1341566/1‑140 (MQ=255)
                                                                                       ggATCAGCCGCCTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCa                                             <  1:1408434/140‑1 (MQ=255)
                                                                                       ggATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCa                                             <  1:1389318/140‑1 (MQ=255)
                                                                                       ggATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCa                                             <  2:346093/140‑1 (MQ=255)
                                                                                       ggATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCa                                             <  1:786317/140‑1 (MQ=255)
                                                                                         aTCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCAtt                                           >  2:763878/1‑140 (MQ=255)
                                                                                               cGACTCGGGATCATCTCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGGAGCAGCATTTTATAAGCGAGAATACGGCATTGATCtt                                     <  2:1262175/140‑1 (MQ=255)
                                                                                               cGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCtt                                     <  2:2316492/140‑1 (MQ=255)
                                                                                               cGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCtt                                     <  2:126514/140‑1 (MQ=255)
                                                                                               cGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCtt                                     <  2:1920973/140‑1 (MQ=255)
                                                                                                   acatgAACATAACATAGAGGAAAAGAAACGAGCCGTTGCTGATCCTGAAAAACATAAAAATTCGTCTTTTCGACGATCATAAAGAATTAAATCAGCCGAAGCAGCATTATAAAAGCGAGAATACGGCATTGATCATGAca                                 >  2:590809/5‑139 (MQ=255)
                                                                                                        aaCATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGaa                            >  2:2058002/1‑140 (MQ=255)
                                                                                                             atCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCATCCGCAGCAGCATTTTATAAGCGAGAATACGGCAATGATCTTGACCCAGAAACTGa                       >  1:329314/1‑140 (MQ=255)
                                                                                                             atCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGa                       <  1:925436/140‑1 (MQ=255)
                                                                                                             atCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGa                       >  1:1442225/1‑140 (MQ=255)
                                                                                                             atCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGa                       >  1:825461/1‑140 (MQ=255)
                                                                                                             atCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGa                       <  1:749969/140‑1 (MQ=255)
                                                                                                             atCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGa                       <  1:2189928/140‑1 (MQ=255)
                                                                                                              tcgtcgAGGAAATGAAACGAGCCGTTGCTTATCCTGAACATCAGAAATATTCGTCTTTTCGCGGCTGATACAGCCTGAAATCAGCCGCAGCATGATTTTATAAGCCAGTATACGGCATTGATCTTGACCCATAAACTGaa                      <  2:583811/140‑1 (MQ=255)
                                                                                                               cgtcgAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGAAg                     >  2:577429/1‑140 (MQ=255)
                                                                                                               cgtcgAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGAAg                     >  2:2124187/1‑140 (MQ=255)
                                                                                                               cgtcgAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGAAg                     >  2:2220263/1‑140 (MQ=255)
                                                                                                               cgtcgAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATCTTATAAGCGAGAATACGGCATGGATCTTGACCCAGAAACTGAAg                     <  2:169469/140‑1 (MQ=255)
                                                                                                               cctcgAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGAAg                     <  2:14655/138‑1 (MQ=255)
                                                                                                                 tcgAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGAAGTc                   >  2:535596/1‑140 (MQ=255)
                                                                                                                 tcgAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGAAGTc                   >  2:622289/1‑140 (MQ=255)
                                                                                                                  cgAGGAAATGAAACGAGCCGTTGCTGATCCTTAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGAAGTCg                  <  1:1851225/140‑1 (MQ=255)
                                                                                                                  cgAGGAAATGAAACGAGCCGTTGCTGATCCTGAAACTCATAAATATTCGTCTGTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGAAGGCg                  <  2:522120/140‑1 (MQ=255)
                                                                                                                  cgAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGAAGTCg                  <  2:541161/140‑1 (MQ=255)
                                                                                                                  cgAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGAAGTCg                  <  2:626595/140‑1 (MQ=255)
                                                                                                                  cgAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGAAGTCg                  <  1:1238295/140‑1 (MQ=255)
                                                                                                                  cgAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGAAGTCg                  <  1:423482/140‑1 (MQ=255)
                                                                                                                  cgAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGAAGTCg                  <  1:590984/140‑1 (MQ=255)
                                                                                                                  cgAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGAAGTCg                  <  1:590809/140‑1 (MQ=255)
                                                                                                                  cgAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGAAGTCg                  <  1:514281/140‑1 (MQ=255)
                                                                                                                  cgAGGAAAGGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGAAGTCg                  <  1:1468256/140‑1 (MQ=255)
                                                                                                                    aGGACATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATCTTCGTCTTTTCGCGGCTCATACAGCCTGAAAGCAGCCGCCGCAGCATTTTCTAAGCGAGAATACGGCCTTGATCTTGCCCCAGCAACGGAAGTCGCt                >  2:2041891/1‑140 (MQ=255)
                                                                                                                               cGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGAAGTCGCTGTATTATTcgg     <  2:445788/140‑1 (MQ=255)
                                                                                                                               cGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGAAGTCGCTGTATTATTcgg     <  2:1958246/140‑1 (MQ=255)
                                                                                                                               cGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGAAGTCGCTGTATTATTcgg     <  2:1067291/140‑1 (MQ=255)
                                                                                                                                gAGCTGTCGCTGATCCTGAGAATCATAAATATTCGTCTTTTCGCGGCTCATACCGTCTGAAGTCAGCGGCTGCTGCATTTTACAAAAGAGAATACGGCATTGATCTGGATCCGGAAACCGAAGTCGCTGTATTATTcggc    <  1:1844741/140‑1 (MQ=255)
                                                                                                                                gAGCTGTCGCTGATCCTGAGAATCATAAATATTCGTCTTTTCGCGGCTCATACCGTCTGAAGTCAGCGGCTGCTGCATATTACAAAAGAGAATACGGCATTGATCTGGATCCGGAAACCGAAGTCGCTGTATTATTcggc    <  1:1341566/140‑1 (MQ=255)
                                                                                                                                 aGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGAAGTCGCTGTATTATTcggcg   >  1:665720/1‑140 (MQ=255)
                                                                                                                                 aGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGAAGTCGCTGTATTATTcggcg   >  1:543605/1‑140 (MQ=255)
                                                                                                                                  gCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGAAGTCGCTGTATTATTcggcgg  <  2:495549/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
TAAACAATTCTTCGCTTCTTTGGTGCAGAAAGTGAACCGAAAGCTCTCAGAAGGACACGACGTTATCAATCTCGGACAGGGAAATCCGGATCAGCCGACTCCGGAACATATCGTCGAGGAAATGAAACGAGCCGTTGCTGATCCTGAAAATCATAAATATTCGTCTTTTCGCGGCTCATACAGCCTGAAATCAGCCGCAGCAGCATTTTATAAGCGAGAATACGGCATTGATCTTGACCCAGAAACTGAAGTCGCTGTATTATTCGGCGG  >  NZ_CP034943/1397352‑1397621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: