Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,418,378 C→T 19.3% intergenic (+109/‑126) EO946_RS07425 → / → pfeT TlpA family protein disulfide reductase/metal‑transporting ATPase PfeT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,418,3780CT19.3% 63.1 / 10.0 31intergenic (+109/‑126)EO946_RS07425/pfeTTlpA family protein disulfide reductase/metal‑transporting ATPase PfeT
Reads supporting (aligned to +/- strand):  ref base C (10/15);  new base T (3/3);  total (13/18)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

GAACAACTGAAAGAATGGACGGAAGAATAGCTGAGAGCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTGATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGT  >  NZ_CP034943/1418240‑1418517
                                                                                                                                           |                                                                                                                                           
gAACAACTGAAAGAATGTACGGAAGAATAGCTGAGAGCACAGACTCTCAGCTTTTTTAATGTAATGAAGAATTGATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTgcg                                                                                                                                            <  2:2279809/140‑1 (MQ=255)
gAACAACTGAAAGAATGGACGGAAGAATAGCTGAGAGCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTGATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTgcg                                                                                                                                            <  1:1926151/140‑1 (MQ=255)
gAACAACTGAAAGAATGGACGGAAGAATAGCTGAGAGCACACACTCTCAGCTTTTTTCATGTAATGAAGAATTGATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTgcg                                                                                                                                            <  1:2314337/140‑1 (MQ=255)
  cagacTGAAAGAATGGACGGAAGAATAGCTGAGAGCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTGATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCa                                                                                                                                          <  1:1629360/137‑1 (MQ=255)
  cagacTGAAAGAATGGACGGAAGAATAGCTGAGAGCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTGATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCa                                                                                                                                          <  1:1629447/137‑1 (MQ=255)
  acaacTTAAAGAATGGACGGAAGAATAGCTGAGAGCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTGATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCa                                                                                                                                          <  1:2135071/140‑1 (MQ=255)
  acaacTGAAAGAATGGACGGAAGAATAGCTGAGAGCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTGATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCa                                                                                                                                          <  2:2222103/140‑1 (MQ=255)
  acaacTGAAAGAATGGACGGAAGAATAGCTGAGAGCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTGATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCa                                                                                                                                          <  2:1171371/140‑1 (MQ=255)
  acaacTGAAAGAATGGACGGAAGAATAGCTGAGAGCACAGACTCTCAGCTATTTTCATGTAATGAAGAATTGATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCa                                                                                                                                          <  2:2402961/140‑1 (MQ=255)
            gAATGGACGGAAGAATAGCTGAGAGCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTGATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTataata                                                                                                                                <  2:2307177/140‑1 (MQ=255)
              aTGGACGGAAGAATAGCTGAGAGCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTGATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTCTAATaaa                                                                                                                              >  2:1444685/1‑140 (MQ=255)
                         aaTAGCTGAGAGCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTGATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCa                                                                                                                   <  2:863697/140‑1 (MQ=255)
                         aaTAGCTGAGAGCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTGATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCa                                                                                                                   <  2:1686008/140‑1 (MQ=255)
                                   agCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTGATGGATGTTAATGATAATTATTATCAAGAAGAAATTAACATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACt                                                                                                         >  1:2018595/1‑140 (MQ=255)
                                   agCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTGATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATTATAAAGGAAGACATCAAGAAGTAACt                                                                                                         <  2:375379/140‑1 (MQ=255)
                                   agCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTGATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACt                                                                                                         <  2:2261663/140‑1 (MQ=255)
                                        aGACTCTCAGCTTTTTTCATATAGAGAAGAAGTGAATAAACATTAATGATAATTATTATCAAAAATAAATTAAAATAATTATAAATGAAATTCTCTTCGTGCGTGCTATAATAAAGGAAGACATCAAGAAATAACTGACg                                                                                                     >  1:77718/1‑140 (MQ=255)
                                        aGACTCTCAGCTTTTTTCATATAGAGAAGAAGTGAATAAACATTAATGATAATTATTATCAAAAAGAAATTAAAATAATTATAATTGAAATTCTCTTCGTGCGTGCTATAATAAAGGAAGACATCAAGAAATAACTGACg                                                                                                     >  1:2290597/1‑140 (MQ=255)
                                        aGACTCTCAGCTTTTTTCATATAGAGAAGAAGTGAATAAACATTAATGATAATTATTATCAAAAAGAAATTAAAATAATTATAATTGAAATTCTCTTCGTGCGTGCTATAATAAAGGAAGACATCAAGAAATAACTGACg                                                                                                     >  1:1298973/1‑140 (MQ=255)
                                               cAGCTTTTTTCATGTAATGAAGAATTGATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCt                                                                                             <  2:364665/140‑1 (MQ=255)
                                               cAGCTTTTTTCATGTAATGAAGAATTGATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCt                                                                                             <  2:2028712/140‑1 (MQ=255)
                                                                                  ttAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAtaga                                                           >  2:1263704/1‑140 (MQ=255)
                                                                                  ttAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAgaga                                                           >  2:98494/1‑140 (MQ=255)
                                                                                    aaTGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGaaa                                                         <  1:459855/140‑1 (MQ=255)
                                                                                      ttaTAATTATTATCAAAAAGAAATTAAAATAATTATAATTGAAATTCTATTAGTGCGTGCTATAATAAAGGAAGACATCAAGAAATAACTGACGATAAAGCTGCCTTTTGGGCAGCGATTTTGTTTTTATAAGAGAAATg                                                       <  2:77718/138‑1 (MQ=255)
                                                                                      tGATAATTATTATCAAAAAGAAATTAAAATAATTATAATTGAAATTCTCTTCGTGCGTGCTATAATAAAGGAAGACATCAAGAAATAACTGACGATAAAGCTGCCTTTTGGGCAGCGATTTTGTTTTTATAAGAGAAATg                                                       <  2:2290597/140‑1 (MQ=255)
                                                                                      tGATAATTATTATCAAAAAGAAATTAAAATAATTATAATTGAAATTCTCTTCGTGCGTGCTATAATAAAGGAAGACATCAAGAAATAACTGACGATAAAGCTGCCTTTTGGGCAGCGATTTTGTTTTTATAAGAGAAATg                                                       <  2:1298973/140‑1 (MQ=255)
                                                                                          aaTTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAga                                                   >  2:760861/1‑140 (MQ=255)
                                                                                          aaTTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTGTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAga                                                   >  2:524689/1‑140 (MQ=255)
                                                                                                       aagaaATTAAAATAATTATATTTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTtct                                      >  2:1041725/1‑140 (MQ=255)
                                                                                                       aagaaATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACGGACGATAAAGCTGCCTTTTGGGCGGCAAGTTTGTTTTTATAAGAGAAATGAAGTTGATTTGCGTtct                                      >  2:1233491/1‑140 (MQ=255)
                                                                                                                      ttataattGAAATTCTTGTTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGgaga                       >  1:204222/1‑140 (MQ=255)
                                                                                                                      ttataattGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGgaga                       >  1:830206/1‑140 (MQ=255)
                                                                                                                                       aTTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAAc      <  2:830206/140‑1 (MQ=255)
                                                                                                                                           cgcATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGt  >  2:1604642/1‑140 (MQ=255)
                                                                                                                                           cgcATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGt  >  2:1488087/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
GAACAACTGAAAGAATGGACGGAAGAATAGCTGAGAGCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTGATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGT  >  NZ_CP034943/1418240‑1418517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: