| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NZ_CP034943 | 1,418,381 | A→G | 20.7% | intergenic (+112/‑123) | EO946_RS07425 → / → pfeT | TlpA family protein disulfide reductase/metal‑transporting ATPase PfeT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP034943 | 1,418,381 | 0 | A | G | 20.7% | 56.4 / 10.6 | 29 | intergenic (+112/‑123) | EO946_RS07425/pfeT | TlpA family protein disulfide reductase/metal‑transporting ATPase PfeT |
| Reads supporting (aligned to +/- strand): ref base A (12/11); new base G (3/3); total (15/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACAACTGAAAGAATGGACGGAAGAATAGCTGAGAGCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTG‑ATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGTTA > NZ_CP034943/1418242‑1418519 | cagacTGAAAGAATGGACGGAAGAATAGCTGAGAGCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTG‑ATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCa < 1:1629360/137‑1 (MQ=255)cagacTGAAAGAATGGACGGAAGAATAGCTGAGAGCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTG‑ATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCa < 1:1629447/137‑1 (MQ=255)acaacTTAAAGAATGGACGGAAGAATAGCTGAGAGCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTG‑ATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCa < 1:2135071/140‑1 (MQ=255)acaacTGAAAGAATGGACGGAAGAATAGCTGAGAGCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTG‑ATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCa < 2:2222103/140‑1 (MQ=255)acaacTGAAAGAATGGACGGAAGAATAGCTGAGAGCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTG‑ATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCa < 2:1171371/140‑1 (MQ=255)acaacTGAAAGAATGGACGGAAGAATAGCTGAGAGCACAGACTCTCAGCTATTTTCATGTAATGAAGAATTG‑ATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCa < 2:2402961/140‑1 (MQ=255) gAATGGACGGAAGAATAGCTGAGAGCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTG‑ATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTataata < 2:2307177/140‑1 (MQ=255) aTGGACGGAAGAATAGCTGAGAGCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTG‑ATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTCTAATaaa > 2:1444685/1‑140 (MQ=255) aaTAGCTGAGAGCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTG‑ATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCa < 2:1686008/140‑1 (MQ=255) aaTAGCTGAGAGCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTG‑ATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCa < 2:863697/140‑1 (MQ=255) agCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTG‑ATGGATGTTAATGATAATTATTATCAAGAAGAAATTAACATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACt > 1:2018595/1‑140 (MQ=255) agCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTG‑ATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATTATAAAGGAAGACATCAAGAAGTAACt < 2:375379/140‑1 (MQ=255) agCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTG‑ATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACt < 2:2261663/140‑1 (MQ=255) aGACTCTCAGCTTTTTTCATATAGAGAAGAAGTGAATAAACATTAATGATAATTATTATCAAAAATAAATTAAAATAATTATAAATGAAATTCTCTTCGTGCGTGCTATAATAAAGGAAGACATCAAGAAATAACTGACg > 1:77718/1‑140 (MQ=255) aGACTCTCAGCTTTTTTCATATAGAGAAGAAGTGAATAAACATTAATGATAATTATTATCAAAAAGAAATTAAAATAATTATAATTGAAATTCTCTTCGTGCGTGCTATAATAAAGGAAGACATCAAGAAATAACTGACg > 1:2290597/1‑140 (MQ=255) aGACTCTCAGCTTTTTTCATATAGAGAAGAAGTGAATAAACATTAATGATAATTATTATCAAAAAGAAATTAAAATAATTATAATTGAAATTCTCTTCGTGCGTGCTATAATAAAGGAAGACATCAAGAAATAACTGACg > 1:1298973/1‑140 (MQ=255) cAGCTTTTTTCATGTAATGAAGAATTG‑ATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCt < 2:364665/140‑1 (MQ=255) cAGCTTTTTTCATGTAATGAAGAATTG‑ATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCt < 2:2028712/140‑1 (MQ=255) ttAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAtaga > 2:1263704/1‑140 (MQ=255) ttAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAgaga > 2:98494/1‑140 (MQ=255) aaTGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGaaa < 1:459855/140‑1 (MQ=255) ttaTAATTATTATCAAAAAGAAATTAAAATAATTATAATTGAAATTCTATTAGTGCGTGCTATAATAAAGGAAGACATCAAGAAATAACTGACGATAAAGCTGCCTTTTGGGCAGCGATTTTGTTTTTATAAGAGAAATg < 2:77718/138‑1 (MQ=255) tGATAATTATTATCAAAAAGAAATTAAAATAATTATAATTGAAATTCTCTTCGTGCGTGCTATAATAAAGGAAGACATCAAGAAATAACTGACGATAAAGCTGCCTTTTGGGCAGCGATTTTGTTTTTATAAGAGAAATg < 2:1298973/140‑1 (MQ=255) tGATAATTATTATCAAAAAGAAATTAAAATAATTATAATTGAAATTCTCTTCGTGCGTGCTATAATAAAGGAAGACATCAAGAAATAACTGACGATAAAGCTGCCTTTTGGGCAGCGATTTTGTTTTTATAAGAGAAATg < 2:2290597/140‑1 (MQ=255) aaTTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAga > 2:760861/1‑140 (MQ=255) aaTTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTGTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAga > 2:524689/1‑140 (MQ=255) aagaaATTAAAATAATTATATTTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTtct > 2:1041725/1‑140 (MQ=255) aagaaATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACGGACGATAAAGCTGCCTTTTGGGCGGCAAGTTTGTTTTTATAAGAGAAATGAAGTTGATTTGCGTtct > 2:1233491/1‑140 (MQ=255) ttataattGAAATTCTTGTTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGgaga > 1:204222/1‑140 (MQ=255) ttataattGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGgaga > 1:830206/1‑140 (MQ=255) aTTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAAc < 2:830206/140‑1 (MQ=255) cgcATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGt > 2:1604642/1‑140 (MQ=255) cgcATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGt > 2:1488087/1‑140 (MQ=255) cATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGTTa < 1:698396/140‑1 (MQ=255) cATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGTTa < 1:1444685/140‑1 (MQ=255) | ACAACTGAAAGAATGGACGGAAGAATAGCTGAGAGCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTG‑ATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGTTA > NZ_CP034943/1418242‑1418519 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |