| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NZ_CP034943 | 1,418,409 | G→A | 12.5% | intergenic (+140/‑95) | EO946_RS07425 → / → pfeT | TlpA family protein disulfide reductase/metal‑transporting ATPase PfeT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP034943 | 1,418,409 | 0 | G | A | 12.5% | 125.1 / 9.2 | 48 | intergenic (+140/‑95) | EO946_RS07425/pfeT | TlpA family protein disulfide reductase/metal‑transporting ATPase PfeT |
| Reads supporting (aligned to +/- strand): ref base G (31/11); new base A (3/3); total (34/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-01 | |||||||||||
AGCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTG‑ATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGTTATCGTTCAACACGATCCGCATGAG > NZ_CP034943/1418275‑1418542 | agCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTG‑ATGGATGTTAATGATAATTATTATCAAGAAGAAATTAACATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACt > 1:2018595/1‑140 (MQ=255)agCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTG‑ATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATTATAAAGGAAGACATCAAGAAGTAACt < 2:375379/140‑1 (MQ=255)agCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTG‑ATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACt < 2:2261663/140‑1 (MQ=255) aGACTCTCAGCTTTTTTCATATAGAGAAGAAGTGAATAAACATTAATGATAATTATTATCAAAAATAAATTAAAATAATTATAAATGAAATTCTCTTCGTGCGTGCTATAATAAAGGAAGACATCAAGAAATAACTGACg > 1:77718/1‑140 (MQ=255) aGACTCTCAGCTTTTTTCATATAGAGAAGAAGTGAATAAACATTAATGATAATTATTATCAAAAAGAAATTAAAATAATTATAATTGAAATTCTCTTCGTGCGTGCTATAATAAAGGAAGACATCAAGAAATAACTGACg > 1:1298973/1‑140 (MQ=255) aGACTCTCAGCTTTTTTCATATAGAGAAGAAGTGAATAAACATTAATGATAATTATTATCAAAAAGAAATTAAAATAATTATAATTGAAATTCTCTTCGTGCGTGCTATAATAAAGGAAGACATCAAGAAATAACTGACg > 1:2290597/1‑140 (MQ=255) cAGCTTTTTTCATGTAATGAAGAATTG‑ATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCt < 2:2028712/140‑1 (MQ=255) cAGCTTTTTTCATGTAATGAAGAATTG‑ATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCt < 2:364665/140‑1 (MQ=255) ttAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAtaga > 2:1263704/1‑140 (MQ=255) ttAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAgaga > 2:98494/1‑140 (MQ=255) aaTGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGaaa < 1:459855/140‑1 (MQ=255) ttaTAATTATTATCAAAAAGAAATTAAAATAATTATAATTGAAATTCTATTAGTGCGTGCTATAATAAAGGAAGACATCAAGAAATAACTGACGATAAAGCTGCCTTTTGGGCAGCGATTTTGTTTTTATAAGAGAAATg < 2:77718/138‑1 (MQ=255) tGATAATTATTATCAAAAAGAAATTAAAATAATTATAATTGAAATTCTCTTCGTGCGTGCTATAATAAAGGAAGACATCAAGAAATAACTGACGATAAAGCTGCCTTTTGGGCAGCGATTTTGTTTTTATAAGAGAAATg < 2:2290597/140‑1 (MQ=255) tGATAATTATTATCAAAAAGAAATTAAAATAATTATAATTGAAATTCTCTTCGTGCGTGCTATAATAAAGGAAGACATCAAGAAATAACTGACGATAAAGCTGCCTTTTGGGCAGCGATTTTGTTTTTATAAGAGAAATg < 2:1298973/140‑1 (MQ=255) aaTTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAga > 2:760861/1‑140 (MQ=255) aaTTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTGTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAga > 2:524689/1‑140 (MQ=255) aagaaATTAAAATAATTATATTTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTtct > 2:1041725/1‑140 (MQ=255) aagaaATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACGGACGATAAAGCTGCCTTTTGGGCGGCAAGTTTGTTTTTATAAGAGAAATGAAGTTGATTTGCGTtct > 2:1233491/1‑140 (MQ=255) ttataattGAAATTCTTGTTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGgaga > 1:204222/1‑140 (MQ=255) ttataattGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGgaga > 1:830206/1‑140 (MQ=255) aTTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAAc < 2:830206/140‑1 (MQ=255) cgcATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGt > 2:1604642/1‑140 (MQ=255) cgcATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGt > 2:1488087/1‑140 (MQ=255) cATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGTTa < 1:1444685/140‑1 (MQ=255) cATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGTTa < 1:698396/140‑1 (MQ=255) aGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAATATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGTTATCGTTCAACACGa > 2:1186838/1‑140 (MQ=255) aGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGatat < 2:804620/113‑1 (MQ=255) aGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGatat > 1:804620/1‑113 (MQ=255) aGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATTAATTAACAAGTTATCGTTCAACAAGa > 2:2270187/1‑140 (MQ=255) aGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGTTATCGTTCAACACGa > 1:802211/1‑140 (MQ=255) aGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGTTATCGTTCAACACGa > 2:639691/1‑140 (MQ=255) aGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGTTATCGTTCAACACGa > 2:48918/1‑140 (MQ=255) aGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGTTATCGTTCAACACGa > 2:1128199/1‑140 (MQ=255) aGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGTTATCGTTCAACACGa > 2:2217980/1‑140 (MQ=255) aGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGTTATCGTTCAACACGa > 2:1221802/1‑140 (MQ=255) aGGAAGACATCAAGAAGTAACAGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAACTAATCGTTCAACACGa > 2:1535242/1‑140 (MQ=255) gAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGTTATCGTTCAACTCGATc > 2:2308394/1‑140 (MQ=255) gAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGTTATCGTTCAACACGATc > 2:468525/1‑140 (MQ=255) gAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGTTATCGTTCAACACGATc > 2:18775/1‑140 (MQ=255) gAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGTTATCGTTCAACACGATc > 2:1838778/1‑140 (MQ=255) gAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGTTATCGTTCAACACGATc > 2:725934/1‑140 (MQ=255) gAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGTTATCGTTCAACACGATc > 1:2412925/1‑140 (MQ=255) aaGACATCAAGAAGTACCTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGTTATCGTTCAACACGATcc > 1:837721/1‑140 (MQ=255) aaGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGTTATCGTTCAACACGATcc > 1:2129406/1‑140 (MQ=255) aGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATACGAGAAATGAAGAGGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGTTATCGTTCAACACGATCCg > 2:456648/1‑140 (MQ=255) gACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAAAAAGTTATAGTTCAACACGATCCGc > 2:896026/1‑140 (MQ=255) tCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGTTATCGTTCAACACGATCCGCATGa < 1:2286145/140‑1 (MQ=255) cAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGTTATCGTTCAACACGATCCGCATGAg < 2:204222/140‑1 (MQ=255) | AGCACAGACTCTCAGCTTTTTTCATGTAATGAAGAATTG‑ATGGATGTTAATGATAATTATTATCAAGAAGAAATTAAAATAATTATAATTGAAATTCTTATTGCGCATGCTATAATAAAGGAAGACATCAAGAAGTAACTGACGATAAAGCTGCCTTTTGGGCGGCAATTTTGTTTTTATAAGAGAAATGAAGATGATTTGCGTTCTCAATTAGAGAGGAGATTTTGATATGAATGAACAAGTTATCGTTCAACACGATCCGCATGAG > NZ_CP034943/1418275‑1418542 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |