Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,566,216 A→G 7.5% N132D (AAT→GAT)  EO946_RS08175 → S8 family serine peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,566,2160AG 7.5% 290.2 / 8.4 95N132D (AAT→GAT) EO946_RS08175S8 family serine peptidase
Reads supporting (aligned to +/- strand):  ref base A (46/41);  new base G (4/3);  total (50/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGAAATCGAAGAAGCTGACTGCCGCTAAGACGGAATATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTGCTTCCAAATGAGAAACGGCAGTTGT  >  NZ_CP034943/1566089‑1566354
                                                                                                                               |                                                                                                                                          
aGCGAAATAGAAGAAGCTGACTGCCGCTAAGACGGAATATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATg                                                                                                                                <  1:1249231/140‑1 (MQ=255)
   gAAATCGAAGAAGCTGTCTGCCGCTAAGACGGAATATCAAAAGCGTTCTGCTGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGCAAGATGTCCTAAAATACTTGAACACCCAGAAAGATAAAGGAAATGCAg                                                                                                                             >  1:1031550/1‑140 (MQ=255)
   gAAATCGAAGAAGCTGTCTGCCGCTAAGACGGAATATCAAAAGCGTTCTGCTGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGCAAGATCTCCTAAAATACTTGAACACCCAGAAAGATAAAGGAAATGCAg                                                                                                                             >  1:12116/1‑140 (MQ=255)
   gAAATCGAAGAAGCTGTCTGCCGCTAAGACGGAATATCAAAAGCGTTCTGCTGTTGTGTCATCGGTAAAAGTCACAGCCGATGAATCCCAGCAAGATGTCCTAAAATACTTGAACACCCAGAAAGATAAAGGAACTGCAg                                                                                                                             >  1:344932/1‑140 (MQ=255)
   gAAATCGAAGAAGCTGACTGCCGCTAAGACGGAATATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAg                                                                                                                             >  2:699237/1‑140 (MQ=255)
   gAAATCGAAGAAGCTGACTGCCGCTAAGACGGAATATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAg                                                                                                                             >  2:64897/1‑140 (MQ=255)
   gAAATCGAAGAAGCTGACTGCCGCTAAGACGGAATATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAg                                                                                                                             >  2:424298/1‑140 (MQ=255)
     aaTCGAAGAAGCTGACTGCCGCTAAGACGGAATATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGAc                                                                                                                           <  2:879214/140‑1 (MQ=255)
     aaTAGAAGCAGCTGACTGCCGCTAAGACGGAATATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGAc                                                                                                                           <  2:1904073/140‑1 (MQ=255)
           agaagCTGACTGCCGCTAAGACGGAATATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAAtt                                                                                                                     <  1:2280990/140‑1 (MQ=255)
              agCTGACTGCCGCTAAGACGGAATATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCAc                                                                                                                  <  2:388800/140‑1 (MQ=255)
              agCTGACTGCCGCTAAGACGGAATATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCAc                                                                                                                  <  1:543420/140‑1 (MQ=255)
              agCTGACTGCCGCTAAGACGGAATATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCAc                                                                                                                  <  1:1487966/140‑1 (MQ=255)
              agCTGACTGCCGCTAAGACGGAATATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCAc                                                                                                                  <  1:2357028/140‑1 (MQ=255)
                cTGACTGCCGCTAAGACGGAATATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCActc                                                                                                                <  1:820407/140‑1 (MQ=255)
                cTGACTGCCGCTAAGACGGAATATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCActc                                                                                                                <  1:690877/140‑1 (MQ=255)
                cTGACTGCCGCTAAGACGGAATATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCActc                                                                                                                <  1:1070376/140‑1 (MQ=255)
                cTGACTGCCGCTAAGACGGAATATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCActc                                                                                                                <  1:2254203/140‑1 (MQ=255)
                   aCTGCCGCTAAGACGGAATATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAACTGCAGACCAAATTCACTCtta                                                                                                             <  1:1124822/140‑1 (MQ=255)
                   aCTGCCGCTAAGACGGAATATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCtta                                                                                                             <  2:645469/140‑1 (MQ=255)
                   aCTGCCGCTAAGACGGAATATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAAGTCACTCtta                                                                                                             <  2:2248411/140‑1 (MQ=255)
                   aCTGCCGCTAAGACGGAATATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGGCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCtta                                                                                                             <  2:1356889/140‑1 (MQ=255)
                        cGCTAAGACGGAATATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATg                                                                                                        <  2:2406641/140‑1 (MQ=255)
                          cTAAGACGGAATATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGACTCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTa                                                                                                      >  2:886511/1‑140 (MQ=255)
                               tctgAATATCACACGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCAGATGAATCCCAGAGCGATGTCTTAACATACTTGATCAGCCAGAAATATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGaa                                                                                                 <  1:1819444/137‑1 (MQ=255)
                               aCGGAATATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGaa                                                                                                 <  1:942204/140‑1 (MQ=255)
                               aCGGAATATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGaa                                                                                                 <  1:1161503/140‑1 (MQ=255)
                                  gAATATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATgg                                                                                              <  2:317772/140‑1 (MQ=255)
                                  gAATATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATgg                                                                                              <  1:1552702/140‑1 (MQ=255)
                                  gAATATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATgg                                                                                              <  1:2393446/140‑1 (MQ=255)
                                   aaTATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATggg                                                                                             >  2:1227119/1‑140 (MQ=255)
                                   aaTATCAAAAGCGTTCTGCCGTTGTGTCATCTGTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATggg                                                                                             >  2:671519/1‑140 (MQ=255)
                                       tCAAAACCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCCATGAATCCCAGAGAGATGTCTTAACATACGTGAACAGCCAGAAAAATAAGCGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCg                                                                                         <  1:2292172/140‑1 (MQ=255)
                                         aaaaGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCttatt                                                                                                           <  1:2312486/120‑1 (MQ=255)
                                         aaaaGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCttatt                                                                                                           >  2:2312486/1‑120 (MQ=255)
                                          aaaGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGACAAATAAGGGAAATGCAGACCAAATTCACTCTTATTCTGTAGTGAATGGGATCGCTg                                                                                      >  2:1979928/1‑140 (MQ=255)
                                                ttCTGCCGTTGTGTCATCTTTAAAAGTCACATCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTATTGAATGGGATCGCTGTTCAtt                                                                                >  1:1382450/1‑139 (MQ=255)
                                                ttCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATg                                                                                >  1:565514/1‑140 (MQ=255)
                                                 tCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGc                                                                               >  1:302170/1‑140 (MQ=255)
                                                          gtgtCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAaaga                                                                      >  2:1309745/1‑140 (MQ=255)
                                                              cATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTg                                                                  <  1:1715344/140‑1 (MQ=255)
                                                               aTCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCActc                                                                                                                >  2:2286657/1‑93 (MQ=255)
                                                               aTCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCActc                                                                                                                <  1:2286657/93‑1 (MQ=255)
                                                               aTCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGTATGGGATCGCTGTTCATGCCTCAAAAGTAGTGa                                                                 >  1:2401762/1‑140 (MQ=255)
                                                               aTCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGa                                                                 >  1:754957/1‑140 (MQ=255)
                                                                           cacaGCCGATGAATCCCAGCAAGATGTCCTAAAATACTTGAACACCCAGAAAGATAAAGGAAATGCAGACCAAATTCATTCTTATTATGTGGTGAACGGGATTGCTGTTCATGCCTCAAAAGAGGTTATGGAAAAAGtgg                                                     >  1:1725982/1‑138 (MQ=255)
                                                                           cacaGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAg                                                     >  2:45081/1‑140 (MQ=255)
                                                                             caGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAgcg                                                   <  1:699237/140‑1 (MQ=255)
                                                                             caGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAgcg                                                   <  1:64897/140‑1 (MQ=255)
                                                                             caGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAgcg                                                   <  1:2256279/140‑1 (MQ=255)
                                                                             caGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAgcg                                                   <  1:424298/140‑1 (MQ=255)
                                                                                   aTGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTc                                             >  1:1072817/1‑140 (MQ=255)
                                                                                     gAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTccc                                           <  2:145542/140‑1 (MQ=255)
                                                                                        tCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGa                                        >  1:1969260/1‑140 (MQ=255)
                                                                                        tCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGGAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGCAAAAGTAGCGCAGTTCCCCGa                                        >  2:1528378/1‑140 (MQ=255)
                                                                                                  aTGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAg                              >  1:1382348/1‑140 (MQ=255)
                                                                                                   tGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAgg                             >  2:400538/1‑140 (MQ=255)
                                                                                                   tGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAgg                             >  2:1764760/1‑140 (MQ=255)
                                                                                                   tGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAgg                             >  1:1151295/1‑140 (MQ=255)
                                                                                                   tGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATCGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAgg                             >  1:2368175/1‑140 (MQ=255)
                                                                                                    gTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTTATGGAATTAGTATATAATTTACCCGATGTGGaaattgt                            <  2:5932/140‑5 (MQ=255)
                                                                                                    gTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGt                            <  2:1117270/140‑1 (MQ=255)
                                                                                                    gTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGt                            <  2:2351456/140‑1 (MQ=255)
                                                                                                     tCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAACTTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTg                           >  2:311552/1‑140 (MQ=255)
                                                                                                     tCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTg                           >  2:136530/1‑140 (MQ=255)
                                                                                                     tCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTg                           >  2:1990016/1‑140 (MQ=255)
                                                                                                     tCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTg                           >  2:1675194/1‑140 (MQ=255)
                                                                                                     tCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTg                           >  2:1836331/1‑140 (MQ=255)
                                                                                                     tCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTg                           >  2:1836341/1‑140 (MQ=255)
                                                                                                             tACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTGATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTGCTTCaaaa                   >  1:884106/1‑140 (MQ=255)
                                                                                                              aCTTGAACAGCCAGTAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTGCTTCCACAt                  >  1:1541757/1‑140 (MQ=255)
                                                                                                               cTTGAACAGCCAGAGAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAgcg                                                   <  2:1553618/106‑1 (MQ=255)
                                                                                                               cTTGAACAGCCAGAGAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAgcg                                                   >  1:1553618/1‑106 (MQ=255)
                                                                                                               cTTGAACAGCCAGAAAAATAAGGTAAATTCAGACCAAATCAACTCTTATTATGTAGTGAATGTGATCTCTGTTCATGACTAAAAAGAAGTGATGTAAAAAGTAGCGCAGTTCCCCGAACTGGACAAGGTGCTTCCAAAtc                 >  1:135416/1‑139 (MQ=255)
                                                                                                               cTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTGCTTCCAAATg                 >  2:965414/1‑140 (MQ=255)
                                                                                                               cTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTGCTTCCAAATg                 >  1:1051045/1‑140 (MQ=255)
                                                                                                               cTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTGCTTCCAAATg                 >  2:2154130/1‑140 (MQ=255)
                                                                                                               cTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTGCTTCCAAATg                 >  1:1051086/1‑140 (MQ=255)
                                                                                                               cTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTGCTTCCAAATg                 >  1:17152/1‑140 (MQ=255)
                                                                                                               cTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTGCTTCCAAATg                 >  1:1910048/1‑140 (MQ=255)
                                                                                                               cTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTGCTTCCAAATg                 >  2:110267/1‑140 (MQ=255)
                                                                                                               cTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTGCTTCCAAATg                 >  1:1816016/1‑140 (MQ=255)
                                                                                                               cTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTAGGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGTAGTGATGGCAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTGCTTCCAAATg                 >  1:1891409/1‑140 (MQ=255)
                                                                                                               cTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTAGTATGTAGTGAATGGGATCGCTGTTCATGCGTCAAAAGCAGGGATGGAAAATGTAGCGCAGTTCCCCGAAGTGGAATAGGTGCTTCCAAGTg                 >  1:1909776/1‑140 (MQ=255)
                                                                                                                        ccAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGACAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTGCTTCCAAATGAGAAACGGc        >  2:400570/1‑140 (MQ=255)
                                                                                                                         cAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTGCTTCCAAATGAGAAACGGCa       <  1:173827/140‑1 (MQ=255)
                                                                                                                          aGAAAGATAAAGGCAATGCAGACCAAATTCATTCTTATTATGTGGTGAACGGGTTTGCTGTTCATGCCTCAAAAGAGGTTATGGAAAAAGTGGTGCAGTTTCCCGAAGTGGAAAAGGTGCTTCCTAATGAGAAACGGCAg      <  2:344932/140‑1 (MQ=255)
                                                                                                                          aGAAAGATAAAGGAAATGCAGACCAAATTCATTCTTATTCTGTGGTGAACGGGATTGCTGTTCATGCCTCAAAAGAGGTTATGGAAAAAGTGGTGCAGTTTCCCGAAGTGGAAAAGGTGCTTCCTAATGAGAAACGGCAg      <  2:12116/140‑1 (MQ=255)
                                                                                                                          aGAAAGATAAAGGAAATGCAGAACAAATTCATTCTTATTATGTGGTGAACGGGATTGCTGTTCATGCCTCAAAAGAGGTTATGGAAAAAGTGGTGCAGTTTCCCGAAGTGGAAAAGGTGCTTCCTAATGAGAAACGGCAg      <  2:1031550/140‑1 (MQ=255)
                                                                                                                          aGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTGCTTCCAAATgaga              >  2:2217688/1‑132 (MQ=255)
                                                                                                                          aGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTGCTTCCAAATgaga              <  1:2217688/132‑1 (MQ=255)
                                                                                                                          aGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTGCTTCCAAATGAGAAACGGCAg      <  1:965414/140‑1 (MQ=255)
                                                                                                                          aGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTGCTTCCAAATGAGAAACGGCAg      <  2:62799/140‑1 (MQ=255)
                                                                                                                          aGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTGCTTCCAAATGAGAAACGGCAg      <  2:1323180/140‑1 (MQ=255)
                                                                                                                          aGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTGCTTCCAAATGAGAAACGGCAg      <  2:2176141/140‑1 (MQ=255)
                                                                                                                              aaaTAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGTAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTGCTTCCAAATGAGAAACGGCAgttgt  <  2:1165463/140‑1 (MQ=255)
                                                                                                                              aaaTAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTGCTTCCAAATGAGAAACGGCAgttgt  <  2:477316/140‑1 (MQ=255)
                                                                                                                              aaaTAAGGCAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGCTCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTGCTTCCAAATGAGAAACGGCAgttgt  <  2:39269/140‑1 (MQ=255)
                                                                                                                               |                                                                                                                                          
AGCGAAATCGAAGAAGCTGACTGCCGCTAAGACGGAATATCAAAAGCGTTCTGCCGTTGTGTCATCTTTAAAAGTCACAGCCGATGAATCCCAGAGAGATGTCTTAACATACTTGAACAGCCAGAAAAATAAGGGAAATGCAGACCAAATTCACTCTTATTATGTAGTGAATGGGATCGCTGTTCATGCCTCAAAAGAAGTGATGGAAAAAGTAGCGCAGTTCCCCGAAGTGGAAAAGGTGCTTCCAAATGAGAAACGGCAGTTGT  >  NZ_CP034943/1566089‑1566354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: