Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 344,560 T→C 5.2% F136F (TTT→TTC putR → proline utilization transcriptional regulator PutR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943344,5600TC 5.2% 435.4 / 9.4 133F136F (TTT→TTCputRproline utilization transcriptional regulator PutR
Reads supporting (aligned to +/- strand):  ref base T (81/45);  new base C (4/3);  total (85/48)
Fisher's exact test for biased strand distribution p-value = 7.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 148 total aligned reads displayed.

AAATTGAAAGCATTGGGTTAAACCAGAGGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACA  >  NZ_CP034943/344421‑344699
                                                                                                                                           |                                                                                                                                           
aaaTTGAAAGCATTGGGTTAAACCAGAGGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCtttt                                                                                                                                             <  1:1056744/140‑1 (MQ=255)
aaaTTGAAAGCATTGGGTTAAACCAGAGGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCtttt                                                                                                                                             <  1:226250/140‑1 (MQ=255)
            ttGGGTTAAACCAGAGGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGaaa                                                                                                                                 <  1:873882/140‑1 (MQ=255)
             tGGGTTAAACCAGAGGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGaaaa                                                                                                                                >  2:1417074/1‑140 (MQ=255)
                    aaCCAGAGGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTta                                                                                                                         >  1:1086800/1‑140 (MQ=255)
                    aaCCAGAGGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAAGCATTta                                                                                                                         >  1:1019004/1‑140 (MQ=255)
                    aaCCAGAGGGTGGTAGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTta                                                                                                                         >  1:1175369/1‑140 (MQ=255)
                         gagGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATaaaa                                                                                                                    >  2:1572318/1‑140 (MQ=255)
                         gagGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATaaaa                                                                                                                    >  2:257015/1‑140 (MQ=255)
                              tggtCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGGCGGGAAAATCATTTATAAAACGAAc                                                                                                               >  2:715500/1‑140 (MQ=255)
                               ggtCGGGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGACGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCAGTTAGAAAACGAACa                                                                                                              >  2:1805193/1‑140 (MQ=255)
                                gtCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACaa                                                                                                             >  1:5990/1‑140 (MQ=255)
                                gtCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACaa                                                                                                             >  1:1457787/1‑140 (MQ=255)
                                gtCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACaa                                                                                                             >  2:2268142/1‑140 (MQ=255)
                                gtCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACaa                                                                                                             >  1:405566/1‑140 (MQ=255)
                                gtCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACaa                                                                                                             >  2:599032/1‑140 (MQ=255)
                                gtCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACaa                                                                                                             >  2:749453/1‑140 (MQ=255)
                                 tCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAg                                                                                                            >  2:320770/1‑140 (MQ=255)
                                       gTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAg                                                                                                      <  1:91775/140‑1 (MQ=255)
                                                    aGGGGAAGTCATGGGCTACATCTGGATTCAGGAGCTGGACCAAAATCTGACGGATGAAGAGCTCGACTTTTTGTACGAGACCTCCTTCCATGTCGGGAAAATCATTTATAAAACGAATAAATTGAAACAGGAAAAGgaag                                                                                         >  1:512300/1‑140 (MQ=255)
                                                    aGGGGAAGTCATGGGCTACATCTGGATTCAGGAGCTGGACCAAAATCTGACGGATGAAGAGCTCGACTTTTTGTACGAGACCTCCTTCCATGTCGGGAAAATCATTTATAAAACGAATAAATTGAAACAGGAAAAGgaag                                                                                         >  1:1278980/1‑140 (MQ=255)
                                                     ggggAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATTTGGGTAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGgaaga                                                                                        <  1:332783/140‑1 (MQ=255)
                                                         aaGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaaaa                                                                                    >  1:2087031/1‑140 (MQ=255)
                                                         aaGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaaaa                                                                                    >  1:1352800/1‑140 (MQ=255)
                                                         aaGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaaaa                                                                                    >  1:1352220/1‑140 (MQ=255)
                                                         aaGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCAGTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaaaa                                                                                    >  1:1553304/1‑140 (MQ=255)
                                                          aGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGACCTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGACAAGGAAGAACAAg                                                                                   >  2:1550829/1‑140 (MQ=255)
                                                          aGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAg                                                                                   >  2:1981343/1‑140 (MQ=255)
                                                          aGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAg                                                                                   >  2:1213220/1‑140 (MQ=255)
                                                          aGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAg                                                                                   >  2:508645/1‑140 (MQ=255)
                                                             cATGGGCTACATCTTGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGTACTGGTCTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCTTTTATAAAACGACCAAGCTGAAGCAGGAAAAGGTAGAAAAAGCgg                                                                                <  2:1653395/140‑1 (MQ=255)
                                                              aTGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTGTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGCAAAAGGAAGAAAAATCGGa                                                                               >  2:207914/1‑140 (MQ=255)
                                                                gggCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGCAAAGGAAGAAAAAGCGGAAg                                                                             >  2:301548/1‑140 (MQ=255)
                                                                gggCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAg                                                                             >  2:129322/1‑140 (MQ=255)
                                                                  gCTACATCTGGGTTCAGGAGCTGGACCAAAATTTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAt                                                                           <  2:146890/140‑1 (MQ=255)
                                                                  gCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAt                                                                           <  2:2398903/140‑1 (MQ=255)
                                                                  gCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAt                                                                           <  1:1648870/140‑1 (MQ=255)
                                                                  gCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAt                                                                           <  1:256806/140‑1 (MQ=255)
                                                                     aCATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAtctc                                                                        >  1:2306252/1‑140 (MQ=255)
                                                                      cATCTGGGTTCAGGAGCTGGAGCAAAATCTGACGGAAGAAGTACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAATTCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAGCTCa                                                                       >  1:1653395/1‑140 (MQ=255)
                                                                      cATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCgg                                                                                <  2:141672/131‑1 (MQ=255)
                                                                      cATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCa                                                                       >  2:804740/1‑140 (MQ=255)
                                                                      cATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCa                                                                       >  2:166829/1‑140 (MQ=255)
                                                                      cATCTGGGTTCAGGACCTGGACCAACATCTGACGGACGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCTTGTCGGGAAAATCATTTATAAAACGAACAAGCGGCCGCAGGCAAAGGCCGAAAAAGCGGAACATCTCa                                                                       >  2:1310169/1‑140 (MQ=255)
                                                                           gggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTaaa                                                                  <  1:588675/140‑1 (MQ=255)
                                                                           gggTTCAGGAGCTGGAACAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTaaa                                                                  <  1:226394/140‑1 (MQ=255)
                                                                            ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaa                                                                                      <  1:1298617/119‑1 (MQ=255)
                                                                            ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaa                                                                                      >  2:1298617/1‑119 (MQ=255)
                                                                            ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAAc                                                                 >  2:53412/1‑140 (MQ=255)
                                                                            ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAAc                                                                 >  2:1915116/1‑140 (MQ=255)
                                                                            ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAAc                                                                 >  2:2049134/1‑140 (MQ=255)
                                                                            ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAAc                                                                 >  2:199675/1‑140 (MQ=255)
                                                                            ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAAc                                                                 >  2:1482957/1‑140 (MQ=255)
                                                                            ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAACGAAGAAAAAGCGGAAGATCACATTAAAc                                                                 >  2:416637/1‑140 (MQ=255)
                                                                            ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGGCGGGAAAATCATTGATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAAc                                                                 >  2:1162843/1‑140 (MQ=255)
                                                                               tCAGGAGCTGGACCAAAATCTGAGGGAAGAAGAACTAGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACggg                                                              <  2:1357432/140‑1 (MQ=255)
                                                                               tCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACggg                                                              <  2:2182117/140‑1 (MQ=255)
                                                                                cAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGc                                                             <  2:1086800/140‑1 (MQ=255)
                                                                                cAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGc                                                             <  2:2361675/140‑1 (MQ=255)
                                                                                 aGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGACAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCg                                                            >  1:1489631/1‑140 (MQ=255)
                                                                                 aGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGc                                                             <  1:1898390/139‑1 (MQ=255)
                                                                                 aGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGc                                                             >  2:1898390/1‑139 (MQ=255)
                                                                                 aGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCg                                                            >  1:2186428/1‑140 (MQ=255)
                                                                                 aGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCg                                                            >  1:1185537/1‑140 (MQ=255)
                                                                                  ggAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGa                                                           <  2:1943849/140‑1 (MQ=255)
                                                                                      cTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTa                                                       >  2:1698153/1‑140 (MQ=255)
                                                                                       tGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTAt                                                      <  2:1175369/140‑1 (MQ=255)
                                                                                        ggACCAAAATCTGACGGACGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGAGGAAGATCTCATTAAACGGGCGATCTATc                                                     >  2:1599909/1‑140 (MQ=255)
                                                                                        ggACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATc                                                     >  1:781061/1‑140 (MQ=255)
                                                                                        ggACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATc                                                     >  1:2111707/1‑140 (MQ=255)
                                                                                        ggACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGAAGGAAAAGGAAGAAAAAGCGGTAGATCTCATTAAAAGGGAGAtctctc                                                     >  2:2035925/1‑140 (MQ=255)
                                                                                          aCCAAACTCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAg                                                   <  2:105420/140‑1 (MQ=255)
                                                                                           ccAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGc                                                  <  1:1365925/140‑1 (MQ=255)
                                                                                              aaaTCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAAt                                               >  2:1730250/1‑140 (MQ=255)
                                                                                                 tCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTa                                            <  1:1244396/140‑1 (MQ=255)
                                                                                                 tCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTa                                            <  2:1575945/140‑1 (MQ=255)
                                                                                                  cTGACGGAAGCAGAACTCGCCTTTTTGTATGAGACCTCTTTTCATGTCGGGAACATCATGTATAAAACGAACACGCTGAAGCAGTAAAAGGAAGACACAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTAc                                           <  2:1447331/140‑1 (MQ=255)
                                                                                                  cTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTAc                                           <  2:2298500/140‑1 (MQ=255)
                                                                                                     aCGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCAc                                        <  1:1443870/140‑1 (MQ=255)
                                                                                                     aCGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCAc                                        <  2:468743/140‑1 (MQ=255)
                                                                                                      cGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACt                                       <  1:455489/140‑1 (MQ=255)
                                                                                                          agaagaaCTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTgaga                                   <  1:1383945/140‑1 (MQ=255)
                                                                                                               aaCTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGaa                              <  1:1274719/140‑1 (MQ=255)
                                                                                                               aaCTCGACTTTTTGTATGAGACCTATTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGaa                              <  1:2402241/140‑1 (MQ=255)
                                                                                                                      cTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGAc                       >  1:217623/1‑140 (MQ=255)
                                                                                                                      cTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGAc                       >  1:723/1‑140 (MQ=255)
                                                                                                                         tttGTACGAGACCTCCTTCCATGTCGGGAAAATCATTTATAAAACGAATAAATTGAAACAGGAAAAGGAAGAAAAAGCAGAGGATCTCATCAAACGGGCGATCTATCAGCAATTTACCTCTGAGAAGGAACTCAGACggg                    <  2:1278619/140‑1 (MQ=255)
                                                                                                                                gagaCCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGa             >  2:233247/1‑140 (MQ=255)
                                                                                                                                gagaCCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGa             >  2:804530/1‑140 (MQ=255)
                                                                                                                                 agaCCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCTGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGaa            <  1:3050/140‑1 (MQ=255)
                                                                                                                                 agaCCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGaa            <  1:1780566/140‑1 (MQ=255)
                                                                                                                                   aCCTCTTTTCATGTCGTGAAAATCATTTATAAAAAGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAg          <  2:1441695/140‑1 (MQ=255)
                                                                                                                                   aCCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAg          >  2:963355/1‑140 (MQ=255)
                                                                                                                                   aCCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAg          >  2:963105/1‑140 (MQ=255)
                                                                                                                                     ctctTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGaa        >  2:1781053/1‑140 (MQ=255)
                                                                                                                                         tttCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCaa    <  1:508645/140‑1 (MQ=255)
                                                                                                                                          ttCATGTCGGGAAAATCAGTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCAATGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAac   >  2:2414198/1‑140 (MQ=255)
                                                                                                                                           tCATTTCGTGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAaca  <  2:1331248/140‑1 (MQ=255)
                                                                                                                                           tCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAaca  <  1:832963/140‑1 (MQ=255)
                                                                                                                                           tCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAaca  <  1:963105/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AAATTGAAAGCATTGGGTTAAACCAGAGGGTGGTCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACA  >  NZ_CP034943/344421‑344699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: