Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,659,112 G→A 7.1% G16S (GGC→AGC)  flgB → flagellar basal body rod protein FlgB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,659,1120GA 7.1% 301.0 / 9.8 99G16S (GGC→AGC) flgBflagellar basal body rod protein FlgB
Reads supporting (aligned to +/- strand):  ref base G (56/36);  new base A (3/4);  total (59/40)
Fisher's exact test for biased strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01

Only 100 of 113 total aligned reads displayed.

TTTATGCCTGTTATTTCTTACAATAAGCATATAGTTTTACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACTATAAGGCGAAAAAAGTCTCTTTCCAAAATTTATTAGATCAAGAATCCTCTCGTCTTGAAGCGATAAAAACAGACTA  >  NZ_CP034943/1658973‑1659251
                                                                                                                                           |                                                                                                                                           
tttATGCCTGTTATTTCTTACAATAAGCATATAGTTTTACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTgg                                                                                                                                             >  1:424181/1‑140 (MQ=255)
tttATGCCTGTCATTTCTTACAATAAGCATATAGTTTTACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTgg                                                                                                                                             >  1:95568/1‑140 (MQ=255)
 ttATGCCTGTTATTTCTTACAATAAGCATATAGTTTTACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAAAGATACAAAATCTTGAAAATGCCTTggg                                                                                                                                            >  1:1556248/1‑140 (MQ=255)
 ttATGCCTGTTATTTCTTACAATAAGCATATAGTTGTACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAATATCTTGAAAATGCCTTggg                                                                                                                                            >  1:923901/1‑140 (MQ=255)
  tATGCCTGTTATTTCTTACAATAAGCATATAGTTTTACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGc                                                                                                                                           >  1:786508/1‑140 (MQ=255)
   aTGCCTGTTATTTCTTACAATAAGCATATAGTTTTACAATTCTCGACACGGATATGGAGGGTGAAATACACTGAAAGGGAGGTACGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCa                                                                                                                                          >  1:1338375/1‑140 (MQ=255)
   aTGCCTGTTATTTCTTACAATAAGCATATAGTTTTACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGACATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAACATGCCTTGGGCa                                                                                                                                          >  1:620066/1‑140 (MQ=255)
   aTGCCTGTTATTTCTTACAATAAGCATATAGTTTTACAATTCTCGACAAGGATATGGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGACCGATACAAAATCTTGAAAATGCCTTGGGcc                                                                                                                                          >  2:903996/1‑139 (MQ=255)
          ttATTTCTTACAATAAGCATATAGTTTTACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGACAATGCCTTGGGCAGAGCGGa                                                                                                                                   >  1:608182/1‑140 (MQ=255)
          ttATTTCTTACAATAAGCATATAGTTTTACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGa                                                                                                                                   >  2:1598763/1‑140 (MQ=255)
          ttATTTCTTACAATAAGCATATAGTTTTACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGa                                                                                                                                   >  1:522542/1‑140 (MQ=255)
          ttATTTCTTACAATAAGCATATAGTTTTACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGa                                                                                                                                   >  1:479440/1‑140 (MQ=255)
              ttCTTACAATAAGCATATAGTTTTACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATAtt                                                                                                                               <  1:2285026/140‑1 (MQ=255)
                     aaTAAGCATATAGTTTTACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCaaa                                                                                                                        <  1:1022348/140‑1 (MQ=255)
                     aaTAAGCATATAGTTTTACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCaaa                                                                                                                        <  1:122028/140‑1 (MQ=255)
                     aaTAAGCATATAGTTTTACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCaaa                                                                                                                        <  1:1763781/140‑1 (MQ=255)
                     aaTAAGCATATAGTTTTACAATTCTCGACAAGGAGATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCaaa                                                                                                                        <  1:28116/140‑1 (MQ=255)
                       tAAGCATATAGTTTTACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCaaaaa                                                                                                                      >  1:1447640/1‑140 (MQ=255)
                       tAAGCATATAGTTTTACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCaaaaa                                                                                                                      >  1:1917638/1‑140 (MQ=255)
                       tAAGCATATAGTTTTACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGGAAGTGGAGTTGAGCTTATTTTCTGGAACGATACAAAATCTTGACAATGCCTTGGGCAGAGCGGATATTAAGCgaaaa                                                                                                                      >  1:1911100/1‑140 (MQ=255)
                         aGCATATAGTTTTACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGt                                                                                                                    >  2:1001614/1‑140 (MQ=255)
                          gCATATAGTTTTACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGAGCAGAGCGGATATTAAGCAAAAAGTc                                                                                                                   <  1:290497/140‑1 (MQ=255)
                            atatAGTTTTACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCAt                                                                                                                 <  2:1767980/140‑1 (MQ=255)
                              ataGTTTTACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATaa                                                                                                               <  1:1106327/140‑1 (MQ=255)
                               taGTTTTACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAAc                                                                                                              >  1:693771/1‑140 (MQ=255)
                                   tttACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACtaat                                                                                                          >  1:1315860/1‑140 (MQ=255)
                                     tACAATTCTCGACCAGGATATTTAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATCTTAAGCAAAAAGTCATAAGtaagat                                                                                                        >  1:1115076/1‑137 (MQ=255)
                                     tACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTACGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACtaataa                                                                                                        >  2:385822/1‑140 (MQ=255)
                                     tACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACtcataa                                                                                                        >  1:1115052/1‑140 (MQ=255)
                                     tACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACtaataa                                                                                                        >  2:508460/1‑140 (MQ=255)
                                     tACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACtaataa                                                                                                        >  2:2417440/1‑140 (MQ=255)
                                     tACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACtaataa                                                                                                        >  1:2208657/1‑140 (MQ=255)
                                     tACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACtaataa                                                                                                        >  1:2216394/1‑140 (MQ=255)
                                      aCAATTCTGGACAAGGATATTGAGGGTGAAAAAAACTGAATTGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTaataat                                                                                                       <  1:1444417/140‑1 (MQ=255)
                                          ttCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAatat                                                                                                     >  1:1323516/1‑138 (MQ=255)
                                          ttCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAatat                                                                                                     <  2:474099/138‑1 (MQ=255)
                                          ttCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAatat                                                                                                     >  1:474099/1‑138 (MQ=255)
                                          ttCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAatat                                                                                                     <  2:1323516/138‑1 (MQ=255)
                                          ttCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCg                                                                                                   <  2:1061587/140‑1 (MQ=255)
                                          ttCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCg                                                                                                   <  2:2091107/140‑1 (MQ=255)
                                            ctcGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCTcc                                                                                                 >  1:2047662/1‑140 (MQ=255)
                                            ctcGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGcc                                                                                                 >  1:2240415/1‑140 (MQ=255)
                                            ctcGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGcc                                                                                                 <  1:2415244/140‑1 (MQ=255)
                                            ctcGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGcc                                                                                                 >  1:266144/1‑140 (MQ=255)
                                            ctcGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGcc                                                                                                 <  1:877098/140‑1 (MQ=255)
                                            ctcGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGcc                                                                                                 <  1:1239691/140‑1 (MQ=255)
                                            ctcGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGcc                                                                                                 <  2:2144192/140‑1 (MQ=255)
                                            ctcGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGcc                                                                                                 >  1:1840084/1‑140 (MQ=255)
                                            ctcGACAAGGATATTGAGGGTGAAAAAAAATGAAATGGAGGTAAGTTGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGcc                                                                                                 <  1:108750/140‑1 (MQ=255)
                                              cGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCaa                                                                                               <  2:360205/140‑1 (MQ=255)
                                              cGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCaa                                                                                               <  2:339250/140‑1 (MQ=255)
                                                        atTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATaca                                                                                     <  2:693771/140‑1 (MQ=255)
                                                        atTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATaca                                                                                     <  1:1385560/140‑1 (MQ=255)
                                                        atTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATaca                                                                                     <  1:1500652/140‑1 (MQ=255)
                                                           gAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCg                                                                                  >  2:1733994/1‑140 (MQ=255)
                                                           gAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCg                                                                                  >  2:687191/1‑140 (MQ=255)
                                                           gAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCg                                                                                  >  2:1074630/1‑140 (MQ=255)
                                                              ggTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCaaa                                                                                                                        >  1:487208/1‑99 (MQ=255)
                                                              ggTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAAc                                                                               >  1:1159842/1‑140 (MQ=255)
                                                              ggTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAAc                                                                               >  2:538448/1‑140 (MQ=255)
                                                              ggTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAAc                                                                               >  1:1409605/1‑140 (MQ=255)
                                                               gTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATATATACACCGaaat                                                                              >  2:1308499/1‑138 (MQ=255)
                                                               gTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACt                                                                              >  1:292960/1‑140 (MQ=255)
                                                               gTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACt                                                                              >  1:2072271/1‑140 (MQ=255)
                                                               gTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATAAAAAATCTTGAAAATGCCTTGGGCATAGCGGATATTAACCAAAAAGTCATAAATAAAAATATCGCCAATATATATACACCGAACt                                                                              >  2:1243922/1‑140 (MQ=255)
                                                                tGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGc                                                                                                                                      <  2:2124733/83‑1 (MQ=255)
                                                                tGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGc                                                                                                                                      >  1:2124733/1‑83 (MQ=255)
                                                                tGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACta                                                                             >  1:112899/1‑140 (MQ=255)
                                                                tGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACta                                                                             >  1:1652801/1‑140 (MQ=255)
                                                                tGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACta                                                                             >  1:1597126/1‑140 (MQ=255)
                                                                tGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACta                                                                             >  1:296485/1‑140 (MQ=255)
                                                                tGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACta                                                                             >  1:112507/1‑140 (MQ=255)
                                                                  aaaaaaaCTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGAGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACtata                                                                           >  2:2285276/1‑140 (MQ=255)
                                                                  aaaaaaaCTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGAGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACtata                                                                           >  2:165166/1‑140 (MQ=255)
                                                                                                gAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACTATAAGGCGAAAAAAGTCTCTTTCCAAAATttat                                             <  1:495987/140‑1 (MQ=255)
                                                                                                gAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACTATAAGGCGAAAAAAGTCTCTTTCCAAAATttat                                             <  1:1074630/140‑1 (MQ=255)
                                                                                                gAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACTATAAGGCGAAAAAAGTCTCTTTCCAAAATttat                                             <  1:1733994/140‑1 (MQ=255)
                                                                                                gAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACTATAAGGCGAAAAAAGTCTCTTTCCAAAATttat                                             <  1:687191/140‑1 (MQ=255)
                                                                                                gAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGAGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACTATAAGGCAAAAAAAGTCTCTTTCCAAAATttat                                             <  1:161138/140‑1 (MQ=255)
                                                                                                gAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGAGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACTATAAGGCAAAAAAAGTCTCTTTCCAAAATttat                                             <  1:165166/140‑1 (MQ=255)
                                                                                                gAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGAGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACTATAAGGCAAAAAAAGTCTCTTTCCAAAATttat                                             <  1:2285276/140‑1 (MQ=255)
                                                                                                 aGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACTATAAGGCGAAAAAAGTCTCTTTCCAAtatttatt                                            >  1:727133/1‑140 (MQ=255)
                                                                                                   cTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACTATAAGGCGAAAAAAGTCTCTTTCCAAAATTTATTAg                                          >  2:201010/1‑140 (MQ=255)
                                                                                                      aTTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACTATAAGGCGAAAAAAGTCTCTTTCCAAAATTTATTAGATc                                       >  1:10611/1‑140 (MQ=255)
                                                                                                      aTTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACTATAAGGCGAAAAAAGTCTCTTTCCAAAATTTATTAGATc                                       <  2:522542/140‑1 (MQ=255)
                                                                                                      aTTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACTATAAGGCGAAAAAAGTCTCTTTCCAAAATTTATTAGATc                                       >  1:384363/1‑140 (MQ=255)
                                                                                                        tttCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACTATAAGGCGAAAAAAGTCTCTTTCCAAAATTTATTAGATCaa                                     <  2:266144/140‑1 (MQ=255)
                                                                                                        tttCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACTATAAGGCGAAAAAAGTCTCTTTCCAAAATTTATTAGATCaa                                     <  2:1840084/140‑1 (MQ=255)
                                                                                                                aCGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACTATAAGGCGAAAAAAGTCTCTTTCCAAAATTTATTAGATCAAGAATCctc                             <  2:2189277/140‑1 (MQ=255)
                                                                                                                aCGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACTATAAGGCGAAAAAAGTCTCTTTCCAAAATTTATTAGATCAAGAATCctc                             <  2:2077901/140‑1 (MQ=255)
                                                                                                                          aTCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACTATAAGGCGAAAAAAGTCTCTTTCCAAAATTTATTAGATCAAGAATCCTCTCGTCTTGaa                   <  2:1989409/140‑1 (MQ=255)
                                                                                                                                        ttGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACTATAAGGCGAAAAAAGTCTCTTTCCAAAATTTATTAGATCAAGAATCCTCTCGTCTTGAAGCGATAAAAACAGa     <  1:1598763/140‑1 (MQ=255)
                                                                                                                                          gggCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACTATAAGGCGAAAAAAGTCTCTTTCCAAAATTTATTAGATCAAGAATCCTCTCGTCTTGAAGCGATAAAAACAGACt   >  2:297651/1‑140 (MQ=255)
                                                                                                                                          gggCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACTATAAGGCGAAAAAAGTCTCTTTCCAAAATTTATTAGATCAAGAATCCTCTCGTCTTGAAGCGATAAAAACAGACt   >  2:504518/1‑140 (MQ=255)
                                                                                                                                          gggCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACTATAAGGCGAAAAAAGTCTCTTTCCAAAATTTATTAGATCAAGAATCCTCTCGTCTTGAAGCGATAAAAACAGACt   >  2:1760288/1‑140 (MQ=255)
                                                                                                                                          gggCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACTATAAGGCGAAAAAAGTCTCTTTCCAAAATTTATTAGATCAAGAATCCTCTCGTCTTGAAGCGATAAAAAAAGACt   >  2:1201566/1‑140 (MQ=255)
                                                                                                                                           ggCAGAGCTGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACTATAAGGCGAAAAAAGTCTCTTTCCAAAATTTATTAGATCAAGAATCCTCTCGTCTTGAAGCGATAAAAACAGACTa  <  2:1625715/140‑1 (MQ=255)
                                                                                                                                           ggCAGAGCGTATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACTATAAGGCGAAAAAAGTCTCTTTCCAAAATTTATTAGATCAAGAATCCTCTCGTCTTGAAGCGATAAAAACAGACTa  <  2:1447640/140‑1 (MQ=255)
                                                                                                                                           ggCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACTATAAGGCGAAAAAAGTCTCTTTCCAAAATTTATTAGATCAAGAATCCTCTCGTCTTGAAGCGATAAAAACAGACTa  <  2:2010024/140‑1 (MQ=255)
                                                                                                                                           ggCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACTATAAGGCGAAAAAAGTCTCTTTCCAAAATTTATTAGATCAAGAATCCTCTCGTCTTGAAGCGATAAAAACAGACTa  <  2:1911100/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TTTATGCCTGTTATTTCTTACAATAAGCATATAGTTTTACAATTCTCGACAAGGATATTGAGGGTGAAAAAAACTGAAATGGAGGTAAGTGGATTTGAGCTTATTTTCTGGAACGATACAAAATCTTGAAAATGCCTTGGGCAGAGCGGATATTAAGCAAAAAGTCATAACTAATAATATCGCCAATATAGATACACCGAACTATAAGGCGAAAAAAGTCTCTTTCCAAAATTTATTAGATCAAGAATCCTCTCGTCTTGAAGCGATAAAAACAGACTA  >  NZ_CP034943/1658973‑1659251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: