Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,684,359 T→C 5.2% D26D (GAT→GAC sigD → RNA polymerase sigma‑28 factor SigD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,684,3590TC 5.2% 499.2 / 11.5 153D26D (GAT→GACsigDRNA polymerase sigma‑28 factor SigD
Reads supporting (aligned to +/- strand):  ref base T (60/85);  new base C (5/3);  total (65/88)
Fisher's exact test for biased strand distribution p-value = 2.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 158 total aligned reads displayed.

GCATATTCGAACTGTTAAACAAGGTGAAAAAACGATTTAATTAAGGTATTAGGGGGATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATGCTTGGTTTATATGATGCCCTTGAAAAATTTGACCCCA  >  NZ_CP034943/1684220‑1684498
                                                                                                                                           |                                                                                                                                           
 cATATTCGAACTGTTAAACAAGGTGAAAAAACGATTTAATTAAGGTATTAGGGGGATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGAtt                                                                                                                                            >  2:733547/1‑140 (MQ=255)
 cATATTCGAACTGTTAAACAAGGTGAAAAAACGATTTAATTAAGGTATTAGGGGGATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGAtt                                                                                                                                            >  1:836478/1‑140 (MQ=255)
 cATATTCGAACTGTTAAACAAGGTGAAAAAACGATTTAATTAAGGTATTAGGGGGATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGAtt                                                                                                                                            >  1:1436739/1‑140 (MQ=255)
      tagAACTGTTAAACAAGGTGAAAAAACGATTTAATTAAGTTATTAGGGGGATAACAATGCAATCCTTGAATTTTGAAGATCATGTGCTTTTGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATg                                                                                                                                       <  2:1160658/138‑1 (MQ=255)
      tCGAACTGTTAAACAAGGTGAAAAAACGATTTAATTAAGGTATTAGGGGGATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATg                                                                                                                                       <  2:1682571/140‑1 (MQ=255)
      tCGAACTGTTAAACAAGGTGAAAAAACGATTTAATTAAGGTATTAGGGGGATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATg                                                                                                                                       <  2:1661012/140‑1 (MQ=255)
        gAACTGTTAAACAAGGTGAAAAAACGATTTAATTAAGGTATTAGGGGGATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATgcg                                                                                                                                     <  1:852825/140‑1 (MQ=255)
        gAACTGTTAAACAAGGTGAAAAAACGATGTAATTAAGGTATTAGGGGGATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATgcg                                                                                                                                     <  2:1764675/140‑1 (MQ=255)
             gTTAAACAAGGTGAAAAAACGATTTAATTAAGGTATTAGGGGGATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGtt                                                                                                                                <  2:1297979/140‑1 (MQ=255)
                aaaCAAGGTGAAAAAACGATTTAATTAAGGTATTAGGGGGATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACa                                                                                                                             >  1:339054/1‑140 (MQ=255)
                  aCAAGGTGAAAAAACGATTTAATTAAGGTATTAGGGGGATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATg                                                                                                                           <  1:2140853/140‑1 (MQ=255)
                      ggTGAAAAAACGATTTAATTAAGGTATTAGGGGGATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGc                                                                                                                       <  2:1898318/140‑1 (MQ=255)
                      ggTGAAAAAACGATTTAATTAAGGTATTAGGGGGATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGc                                                                                                                       <  1:998071/140‑1 (MQ=255)
                      ggTGAAAAAACGATTTAATTAAGGTATTAGGGGGATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGc                                                                                                                       <  1:1989435/140‑1 (MQ=255)
                      ggTGAAAAAACGATTTAATTAAGGTATTAGGGGGATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGc                                                                                                                       <  1:1298939/140‑1 (MQ=255)
                      ggTGAAAAAACGATTTAATTAAGGTATTAGGGGGATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGc                                                                                                                       >  1:721849/1‑140 (MQ=255)
                      ggTGAAAAAACGATTTAATTAAGGTATTAGGGGGATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGACTTAATGCGCCGTTACATGCCGc                                                                                                                       >  1:364308/1‑140 (MQ=255)
                      ggTGAAAAAACGATTTAATTAAGGTATGAGGGGGATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGc                                                                                                                       <  1:1589545/140‑1 (MQ=255)
                       gTGAAAAAACGATTTAATTAAGGTATTAGGGGGATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCt                                                                                                                      <  2:522951/140‑1 (MQ=255)
                        tGAAAAAACGATTTAATTAAGGTATTAGGGGGATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCtt                                                                                                                     >  2:23391/1‑140 (MQ=255)
                          aaaaaaCGATTTAATTAAGGTATTAGGGGGATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGAGCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGt                                                                                                                   <  1:2102710/140‑1 (MQ=255)
                               aCGATTTAATTAAGGTATTAGGGGGATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCGTGTCACat                                                                                                              <  1:610154/140‑1 (MQ=255)
                                     taattaaGGTATTAGGGGGATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATg                                                                                                        <  1:1738449/140‑1 (MQ=255)
                                     taattaaGGTATTAGGGGGATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATg                                                                                                        <  1:1573472/140‑1 (MQ=255)
                                         taaGGTATCAGGGGGATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAgg                                                                                                    <  1:2211448/140‑1 (MQ=255)
                                               aTTAGGGGGATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCAGGTGACGATTTAATGCGCCGTTACATGCCGATTGTCACATATCATGTAGGCAGAAt                                                                                              <  1:2303611/140‑1 (MQ=255)
                                                      ggATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATgcg                                                                                                                                     >  1:2433880/1‑94 (MQ=255)
                                                      ggATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTc                                                                                       >  1:1026502/1‑140 (MQ=255)
                                                       gATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCg                                                                                      <  2:648260/140‑1 (MQ=255)
                                                        aTAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGTAACGATCCTAAAGCCGGTGACGATTTAATTCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCgg                                                                                     >  1:494216/1‑140 (MQ=255)
                                                        aTAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCgg                                                                                     >  1:2074081/1‑140 (MQ=255)
                                                        aTAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCgg                                                                                     >  2:551352/1‑140 (MQ=255)
                                                        aTAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCgg                                                                                     >  1:530917/1‑140 (MQ=255)
                                                        aTAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCgg                                                                                     >  2:753626/1‑140 (MQ=255)
                                                        aTAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCgg                                                                                     >  1:142665/1‑140 (MQ=255)
                                                        aTAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCgg                                                                                     >  2:385932/1‑140 (MQ=255)
                                                        aTAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCgg                                                                                     >  2:455024/1‑140 (MQ=255)
                                                        aTAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCgg                                                                                     >  2:44075/1‑140 (MQ=255)
                                                         tAACAATTCAATCCTGGAATTATGAATATCAGGTGCTTTGGACGCGCTGGAAAGAGTGTAAAGAGCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATCTCCTGTAGGCAGAATTTCTGTCGGa                                                                                    <  1:1302845/140‑1 (MQ=255)
                                                         tAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGa                                                                                    <  1:1818307/140‑1 (MQ=255)
                                                         tAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGa                                                                                    <  1:1723608/140‑1 (MQ=255)
                                                              aTGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGACAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTgac                                                                               >  1:1455711/1‑138 (MQ=255)
                                                              aTGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGcc                                                                               >  1:1390756/1‑140 (MQ=255)
                                                              aTGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGcc                                                                               >  1:1390762/1‑140 (MQ=255)
                                                               tGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAACGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCg                                                                              >  2:305254/1‑140 (MQ=255)
                                                               tGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCg                                                                              >  2:3520/1‑140 (MQ=255)
                                                                  aaTCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGaaa                                                                           >  2:1966598/1‑140 (MQ=255)
                                                                    tCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATg                                                                                                                           <  2:2248617/90‑1 (MQ=255)
                                                                    tCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATg                                                                                                                           >  1:2248617/1‑90 (MQ=255)
                                                                    tCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATc                                                                         >  2:2121742/1‑140 (MQ=255)
                                                                    tCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGACTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATc                                                                         >  2:1652322/1‑140 (MQ=255)
                                                                      cTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAg                                                                       >  2:1859364/1‑140 (MQ=255)
                                                                      cTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAg                                                                       >  2:2328098/1‑140 (MQ=255)
                                                                       ttGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGt                                                                      >  2:2212368/1‑140 (MQ=255)
                                                                         gAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGACTTAATGCGCCGTTACATGCCGc                                                                                                                       >  1:787100/1‑89 (MQ=255)
                                                                         gAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGACTTAATGCGCCGTTACATGCCGc                                                                                                                       <  2:787100/89‑1 (MQ=255)
                                                                              aTGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATc                                                                         >  2:2066978/1‑130 (MQ=255)
                                                                              aTGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATc                                                                         <  1:2066978/130‑1 (MQ=255)
                                                                               tGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGACTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAg                                                              <  2:85977/140‑1 (MQ=255)
                                                                                   gATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGa                                                          <  2:1791862/140‑1 (MQ=255)
                                                                                       aGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCtt                                                      <  2:1436739/140‑1 (MQ=255)
                                                                                       aGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCtt                                                      <  2:836478/140‑1 (MQ=255)
                                                                                         gTGCTTTGGTCGCGCTGGATAGAGTGGAATGATCGTAAAGCCGTGTACGATTTAATGGGCCGTTAGATGCGGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTAt                                                    <  2:1250642/140‑1 (MQ=255)
                                                                                         gTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATCTCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTAt                                                    <  1:1761132/140‑1 (MQ=255)
                                                                                            cTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAg                                                 <  2:1543224/140‑1 (MQ=255)
                                                                                              ttGGACGCGCTGGAAAGAGTGGAAAGATCCGAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCGTGTCACATATCATGTAGGCAGCATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGCTCTTATGAGcc                                               <  1:2321097/140‑1 (MQ=255)
                                                                                                 gACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTg                                            >  2:1051713/1‑140 (MQ=255)
                                                                                                 gACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTg                                            >  2:505195/1‑140 (MQ=255)
                                                                                                   cgcgCTGGAAAGAGTGGACAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAACTCAGTGCATAAAGACGATCTTATGAGCCTTGGt                                          <  1:1006003/140‑1 (MQ=255)
                                                                                                     cgcTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTAt                                        <  2:1723629/140‑1 (MQ=255)
                                                                                                      gcTGGAAACAGTGGAAAGATCCTAAAGCCGGTGACGACTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATg                                       >  2:595504/1‑140 (MQ=255)
                                                                                                       cTGGAAAGAGTGTAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATGc                                      <  2:70481/140‑1 (MQ=255)
                                                                                                       cTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATc                                                                         >  1:1306851/1‑105 (MQ=255)
                                                                                                       cTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATc                                                                         <  2:767998/105‑1 (MQ=255)
                                                                                                       cTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATc                                                                         >  1:767998/1‑105 (MQ=255)
                                                                                                       cTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATGc                                      <  2:723982/140‑1 (MQ=255)
                                                                                                        tGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATGCt                                     <  2:339054/140‑1 (MQ=255)
                                                                                                        tGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATGCt                                     <  1:1207124/140‑1 (MQ=255)
                                                                                                        tGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATGCt                                     <  2:1586459/140‑1 (MQ=255)
                                                                                                        tGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATGCt                                     <  2:2196603/140‑1 (MQ=255)
                                                                                                        tGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATGCt                                     <  1:883732/140‑1 (MQ=255)
                                                                                                        tGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATGCt                                     <  1:656555/140‑1 (MQ=255)
                                                                                                        tGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATGCt                                     <  1:288078/140‑1 (MQ=255)
                                                                                                        tGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATGCt                                     <  1:2223265/140‑1 (MQ=255)
                                                                                                        aggAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATGCt                                     <  1:1806413/139‑1 (MQ=255)
                                                                                                         ggAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATGCtt                                    <  1:90946/140‑1 (MQ=255)
                                                                                                            aaGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATGCTTGGt                                 <  1:753626/140‑1 (MQ=255)
                                                                                                            aaGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATGCTTGGt                                 <  1:44075/140‑1 (MQ=255)
                                                                                                              gagTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATGCTTGGttt                               <  2:348103/140‑1 (MQ=255)
                                                                                                               agTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATGCTTGGt                                 <  2:950444/137‑1 (MQ=255)
                                                                                                               agTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATGCTTGGt                                 >  1:950444/1‑137 (MQ=255)
                                                                                                               agTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCACAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATGCTTGGTTta                              >  1:1375845/1‑140 (MQ=255)
                                                                                                                  ggAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATGCTTGGTTTatat                           >  2:201985/1‑140 (MQ=255)
                                                                                                                    aaaGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGAAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATGCTTGGTTTATatga                         >  1:1607520/1‑140 (MQ=255)
                                                                                                                           cTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATGCTTGGTTTATATGATGCCCtt                  >  2:1865523/1‑140 (MQ=255)
                                                                                                                                   ggTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATGCTTGGTTTATATGATGCCCTTGAAAAAtt          >  1:556368/1‑140 (MQ=255)
                                                                                                                                    gTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATGCTTGGTTTATATGATGCCCTTGAAAAAttt         >  1:1446383/1‑140 (MQ=255)
                                                                                                                                    gTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATGCTTGGTTTATATGATGCCCTTGAAAAAttt         >  2:1025801/1‑140 (MQ=255)
                                                                                                                                    gTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATGCTTGGTGTATATGATGCCCTTGAAAAAttt         >  2:1580128/1‑140 (MQ=255)
                                                                                                                                           tttAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATGCTTGGTTTATATGATGCCCTTGAAAAATTTGACCCCa  >  2:705687/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
GCATATTCGAACTGTTAAACAAGGTGAAAAAACGATTTAATTAAGGTATTAGGGGGATAACAATGCAATCCTTGAATTATGAAGATCAGGTGCTTTGGACGCGCTGGAAAGAGTGGAAAGATCCTAAAGCCGGTGACGATTTAATGCGCCGTTACATGCCGCTTGTCACATATCATGTAGGCAGAATTTCTGTCGGACTGCCGAAATCAGTGCATAAAGACGATCTTATGAGCCTTGGTATGCTTGGTTTATATGATGCCCTTGAAAAATTTGACCCCA  >  NZ_CP034943/1684220‑1684498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: