Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,704,957 A→C 6.5% Q94H (CAA→CAC truB → tRNA pseudouridine(55) synthase TruB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,704,9570AC 6.5% 359.6 / 7.7 114Q94H (CAA→CACtruBtRNA pseudouridine(55) synthase TruB
Reads supporting (aligned to +/- strand):  ref base A (45/61);  new base C (5/3);  total (50/64)
Fisher's exact test for biased strand distribution p-value = 2.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

TGTGGGAAGAGCGACAAAAATTGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAA  >  NZ_CP034943/1704822‑1705095
                                                                                                                                       |                                                                                                                                          
tgtgGGAAGAGCGACAAAAATTGTCGAATACTTAACTGAAAAGTCTAAAACGTATGTTGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACa                                                                                                                                        >  2:1123427/1‑140 (MQ=255)
  tgGGAAGAGCGACAAAAATTGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACAtt                                                                                                                                      <  2:2260497/140‑1 (MQ=255)
    ggAAGAGCGACAAAAATTGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGTCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGa                                                                                                                                    >  2:2432722/1‑140 (MQ=255)
    ggAAGAGCGACAAAAATTGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACAGTGa                                                                                                                                    >  2:1773651/1‑140 (MQ=255)
         agCGACAAAAATTGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAg                                                                                                                               <  1:1707695/140‑1 (MQ=255)
         agCGACAAAAATTGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAg                                                                                                                               <  1:146018/140‑1 (MQ=255)
         agCGACAAAAATTGTCGAATACTGAACTGAAAAGTCTAAAACGTATGATGCCGAAATAACTCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTAAATCAAGACATTGATAAAg                                                                                                                               <  1:1365639/140‑1 (MQ=255)
          gCGACAAAAATTGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGc                                                                                                                              >  1:1269682/1‑140 (MQ=255)
          gCGACAAAAATTGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGc                                                                                                                              >  2:45013/1‑140 (MQ=255)
          gCGACAAAAATTGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGc                                                                                                                              >  1:41925/1‑140 (MQ=255)
           cGACAAAAATTGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCg                                                                                                                             >  2:884471/1‑140 (MQ=255)
             aCAAAAATTGTCTAATAATTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAATCCTGTCAATCAAGACATTGATAAAGCGgt                                                                                                                           <  1:1338135/140‑1 (MQ=255)
             aCAAAAATTGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGgt                                                                                                                           <  1:1810199/140‑1 (MQ=255)
             aCAAAAATTGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGgt                                                                                                                           <  1:2246490/140‑1 (MQ=255)
             aCAAAAATTGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGgt                                                                                                                           <  1:1809937/140‑1 (MQ=255)
             aCAAAAATTGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGgt                                                                                                                           <  2:2270505/140‑1 (MQ=255)
             aCAAAAATTGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGgt                                                                                                                           <  2:1083366/140‑1 (MQ=255)
             aCAAAAATTGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGgt                                                                                                                           <  1:1730133/140‑1 (MQ=255)
             aCAAAAATTGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGgt                                                                                                                           <  2:1322624/140‑1 (MQ=255)
                     tGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGaaa                                                                                                                   <  2:2099599/140‑1 (MQ=255)
                            tACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGtt                                                                                                            >  2:1896826/1‑140 (MQ=255)
                               ttAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTaaa                                                                                                         <  2:698824/140‑1 (MQ=255)
                                tAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAACGCGGTAGTAGAAAAGGTGTTAAAc                                                                                                        <  1:1009875/140‑1 (MQ=255)
                                tAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAAc                                                                                                        <  1:2689/140‑1 (MQ=255)
                                tAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAAc                                                                                                        <  2:1397327/140‑1 (MQ=255)
                                tAACTGAAAAGTCTAAAACGTATGAGGCGGAAATAACGCTTGGCTTTTCAACGACAACTGCGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAAc                                                                                                        <  2:1274948/140‑1 (MQ=255)
                                tAACTGAAAAGTCGAAAACGGCTGATGCGGAAATAACGCTTGGCTTTTCAACTACAACGGACGATCAAACGGGCGAAACCGTTGAAACAAAGCCTGTCAATCACGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAAc                                                                                                        <  1:1132203/140‑1 (MQ=255)
                                      aaaaGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTa                                                                                                  <  2:1843283/140‑1 (MQ=255)
                                        aaGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTaaa                                                                                                >  2:312006/1‑140 (MQ=255)
                                         aGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAg                                                                                               >  2:607168/1‑140 (MQ=255)
                                         aGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAg                                                                                               >  2:2270343/1‑140 (MQ=255)
                                                 aCGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACa                                                                                       <  2:1351461/140‑1 (MQ=255)
                                                 aCGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACa                                                                                       <  2:710580/140‑1 (MQ=255)
                                                            ggAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtc                                                                            <  2:1670312/140‑1 (MQ=255)
                                                            ggAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtc                                                                            <  2:1767522/140‑1 (MQ=255)
                                                            ggAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtc                                                                            <  2:387829/140‑1 (MQ=255)
                                                             gAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTATAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtcc                                                                           >  1:2078402/1‑140 (MQ=255)
                                                             gAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtcc                                                                           >  2:2355822/1‑140 (MQ=255)
                                                             gAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtcc                                                                           >  2:2292654/1‑140 (MQ=255)
                                                             gAAATAACGCTGGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACACAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTCAACAAGTACAAATacc                                                                           >  1:1586640/1‑140 (MQ=255)
                                                              ataTAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTCAAGGGTAAACAAGAACAAATtcct                                                                          <  1:1280400/138‑1 (MQ=255)
                                                              aaaGAACTCTTGGCTTATCAACGACAACTGAGCATCACACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtcct                                                                          <  1:1353175/140‑1 (MQ=255)
                                                                 tAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTccc                                                                       <  2:670791/140‑1 (MQ=255)
                                                                   aCGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGaaa                                                                                                                   >  2:2156247/1‑94 (MQ=255)
                                                                   aCGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGaaa                                                                                                                   <  1:889979/94‑1 (MQ=255)
                                                                   aCGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGaaa                                                                                                                   <  1:2156247/94‑1 (MQ=255)
                                                                   aCGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGaaa                                                                                                                   >  2:889979/1‑94 (MQ=255)
                                                                   aCGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCAt                                                                     >  1:569549/1‑140 (MQ=255)
                                                                    cGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTCGTAGAACTGGTGTTAAACAGTTTCAAGGGTAAACAAGAGCAAATTCCTCCCATg                                                                    >  1:360355/1‑140 (MQ=255)
                                                                        tGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAACCAAAGCCTGTCAATCAAGACAGTGATAAAGCGGGAGTAGAAAAGGTGTTAATCAGTTTAAAGGGTACACAAGAACAAATTCCTCCCATGTAtt                                                                >  1:1661484/1‑140 (MQ=255)
                                                                        tGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTAtt                                                                >  1:1735548/1‑140 (MQ=255)
                                                                        tGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTAtt                                                                >  1:1860263/1‑140 (MQ=255)
                                                                        tGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTAtt                                                                >  1:291116/1‑140 (MQ=255)
                                                                        tGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTAtt                                                                >  2:62353/1‑140 (MQ=255)
                                                                        tGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTAtt                                                                >  1:1318597/1‑140 (MQ=255)
                                                                         ggCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTc                                                               <  1:2273289/140‑1 (MQ=255)
                                                                         ggCATTTCAACGACAACTGATGATCAAACGGGCTAAACGGTTGAAACAAAGCCTTTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTc                                                               <  2:2078402/140‑1 (MQ=255)
                                                                              ttCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAAACCAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAAGTCCTCCCATGTATTCCGCTg                                                          <  1:2241367/140‑1 (MQ=255)
                                                                               tCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACCTTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGt                                                         >  1:1870780/1‑140 (MQ=255)
                                                                                      cAACTGAGCATCAAACGGGCGAAACGGTTGAAACTAAGCCTGTCAATCAAGACATTGATAACGCGGTAGTAGAATAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAACTTCCTCCCATGTATTCCGCTGTAAAAGTa                                                  <  2:1658981/140‑1 (MQ=255)
                                                                                              gATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCaa                                          >  2:1445797/1‑140 (MQ=255)
                                                                                              gATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCaa                                          >  2:1703167/1‑140 (MQ=255)
                                                                                              gATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCaa                                          <  1:607168/140‑1 (MQ=255)
                                                                                              gATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCaa                                          <  1:2270343/140‑1 (MQ=255)
                                                                                                 cAAACCGGAGAAACGGTGGAAACAAAGCCTGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCa                                           >  2:49753/1‑136 (MQ=255)
                                                                                                 cAAACCGGAGAAACGGTGGAAACAAAGCCTGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCa                                           <  1:1249608/136‑1 (MQ=255)
                                                                                                 cAAACCGGAGAAACGGTGGAAACAAAGCCTGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCa                                           <  1:49753/136‑1 (MQ=255)
                                                                                                          gAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGa                              <  2:287186/140‑1 (MQ=255)
                                                                                                          gAAACGGTTGAAACAAAGCATGTCAATAAAGACATTGATAAAGCGGTAGTAGAAAAGTTGTTAAACAGTTTAAAGGGTAAAAAAGAACAAATTCCTCCCATGTATTCAGCTGTAAAAGTAAACGGCAAAAAATTATACGa                              <  2:287228/140‑1 (MQ=255)
                                                                                                             aCGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTa                           <  1:284029/140‑1 (MQ=255)
                                                                                                               ggTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACg                         <  2:1964190/140‑1 (MQ=255)
                                                                                                                gTTGAAACAAAGCCTGTCAATCTAGCCATTGATTAAGCGGGAGTAGAAAAGGTGTTTAACAGTTTAAAGGTTATCCATTAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTTAAGGGCTAAAAAGTATACGAGTACGc                        <  2:1114208/140‑1 (MQ=255)
                                                                                                                gTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCCTGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGc                        <  2:315434/140‑1 (MQ=255)
                                                                                                                       cAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAAAAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCa                 <  1:1461947/140‑1 (MQ=255)
                                                                                                                          aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGACAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCCGGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAggg              >  1:815284/1‑140 (MQ=255)
                                                                                                                          aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAggg              >  2:323004/1‑140 (MQ=255)
                                                                                                                          aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAggg              >  1:2182150/1‑140 (MQ=255)
                                                                                                                          aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAggg              >  2:2004007/1‑140 (MQ=255)
                                                                                                                          aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAggg              >  2:1529310/1‑140 (MQ=255)
                                                                                                                          aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATAATACGAGTACGCACGAGCAggg              >  2:795255/1‑140 (MQ=255)
                                                                                                                           gCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGa             <  2:1860263/140‑1 (MQ=255)
                                                                                                                           gCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGa             <  2:1747095/140‑1 (MQ=255)
                                                                                                                           gCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGa             <  2:1493648/140‑1 (MQ=255)
                                                                                                                            ccTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGAt            <  1:1703167/140‑1 (MQ=255)
                                                                                                                            ccTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGAt            <  1:990313/140‑1 (MQ=255)
                                                                                                                            ccTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGAt            <  1:1445797/140‑1 (MQ=255)
                                                                                                                            ccTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGAt            <  1:496222/140‑1 (MQ=255)
                                                                                                                              tGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAATACGCAAGAGCAGGGATTg          >  2:1249879/1‑140 (MQ=255)
                                                                                                                              tGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAATACGCAAGAGCAGGGATTg          >  2:2098156/1‑140 (MQ=255)
                                                                                                                               gTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGa         >  2:592867/1‑140 (MQ=255)
                                                                                                                               gTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGa         >  1:724685/1‑140 (MQ=255)
                                                                                                                               gTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGa         >  2:795812/1‑140 (MQ=255)
                                                                                                                               gTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGa         >  2:925529/1‑140 (MQ=255)
                                                                                                                               gTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGa         >  2:957286/1‑140 (MQ=255)
                                                                                                                                tcaatcaaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGaa        >  1:295359/1‑140 (MQ=255)
                                                                                                                                tcaatcaaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGaa        >  1:2082564/1‑140 (MQ=255)
                                                                                                                                tcaatcaaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGaa        >  1:2052236/1‑140 (MQ=255)
                                                                                                                                tcaatcaaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGaa        >  1:1305249/1‑140 (MQ=255)
                                                                                                                                      aaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGaa  >  2:395535/1‑140 (MQ=255)
                                                                                                                                      aaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTACTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGaa  >  2:1024465/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                          
TGTGGGAAGAGCGACAAAAATTGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAA  >  NZ_CP034943/1704822‑1705095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: