Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,704,974 T→A 6.3% V100E (GTA→GAA) ‡ truB → tRNA pseudouridine(55) synthase TruB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,704,9740TA 6.3% 362.0 / 12.4 112V100E (GTA→GAA) ‡truBtRNA pseudouridine(55) synthase TruB
Reads supporting (aligned to +/- strand):  ref base T (49/54);  new base A (5/2);  total (55/57)
Fisher's exact test for biased strand distribution p-value = 2.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

Only 100 of 123 total aligned reads displayed.

ACAAAAATTGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGAT  >  NZ_CP034943/1704835‑1705112
                                                                                                                                           |                                                                                                                                          
aCAAAAATTGTCTAATAATTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAATCCTGTCAATCAAGACATTGATAAAGCGgt                                                                                                                                            <  1:1338135/140‑1 (MQ=255)
aCAAAAATTGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGgt                                                                                                                                            <  2:1083366/140‑1 (MQ=255)
aCAAAAATTGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGgt                                                                                                                                            <  1:2246490/140‑1 (MQ=255)
aCAAAAATTGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGgt                                                                                                                                            <  1:1810199/140‑1 (MQ=255)
aCAAAAATTGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGgt                                                                                                                                            <  1:1809937/140‑1 (MQ=255)
aCAAAAATTGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGgt                                                                                                                                            <  2:2270505/140‑1 (MQ=255)
aCAAAAAGTGTCGAATACTTAACTGAAAAGTCTAAACCGGATGATGCGGAAATAACTCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGgt                                                                                                                                            <  2:1399779/140‑1 (MQ=255)
        tGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGaaa                                                                                                                                    <  2:2099599/140‑1 (MQ=255)
               tACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGtt                                                                                                                             >  2:1896826/1‑140 (MQ=255)
                  ttAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTaaa                                                                                                                          <  2:31433/140‑1 (MQ=255)
                  ttAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTaaa                                                                                                                          <  2:698824/140‑1 (MQ=255)
                   tAACTGAAAATTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAAc                                                                                                                         <  2:1825313/140‑1 (MQ=255)
                   tAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAACGCGGTAGTAGAAAAGGTGTTAAAc                                                                                                                         <  1:1009875/140‑1 (MQ=255)
                   tAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAAc                                                                                                                         <  1:2689/140‑1 (MQ=255)
                   tAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAAc                                                                                                                         <  2:224797/140‑1 (MQ=255)
                   tAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAAc                                                                                                                         <  2:1397327/140‑1 (MQ=255)
                   tAACTGAAAAGTCTAAAACGTATGAGGCGGAAATAACGCTTGGCTTTTCAACGACAACTGCGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAAc                                                                                                                         <  2:1274948/140‑1 (MQ=255)
                   tAACTGAAAAGTCGAAAACGGCTGATGCGGAAATAACGCTTGGCTTTTCAACTACAACGGACGATCAAACGGGCGAAACCGTTGAAACAAAGCCTGTCAATCACGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAAc                                                                                                                         <  1:1132203/140‑1 (MQ=255)
                         aaaaGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTa                                                                                                                   >  2:860171/1‑140 (MQ=255)
                         aaaaGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTa                                                                                                                   <  2:1843283/140‑1 (MQ=255)
                           aaGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTaaa                                                                                                                 >  2:312006/1‑140 (MQ=255)
                            aGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAg                                                                                                                >  2:607168/1‑140 (MQ=255)
                            aGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAg                                                                                                                >  2:2270343/1‑140 (MQ=255)
                                    aCGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACa                                                                                                        <  2:710580/140‑1 (MQ=255)
                                    aCGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACa                                                                                                        <  2:1351461/140‑1 (MQ=255)
                                               ggAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtc                                                                                             <  2:1670312/140‑1 (MQ=255)
                                               ggAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtc                                                                                             <  2:387829/140‑1 (MQ=255)
                                               ggAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtc                                                                                             <  2:1767522/140‑1 (MQ=255)
                                                gAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTATAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtcc                                                                                            >  1:2078402/1‑140 (MQ=255)
                                                gAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtcc                                                                                            >  1:1297138/1‑140 (MQ=255)
                                                gAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtcc                                                                                            >  2:2355822/1‑140 (MQ=255)
                                                gAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtcc                                                                                            >  2:2292654/1‑140 (MQ=255)
                                                gAAATAACGCTGGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACACAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTCAACAAGTACAAATacc                                                                                            >  1:1586640/1‑140 (MQ=255)
                                                 ataTAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTCAAGGGTAAACAAGAACAAATtcct                                                                                           <  1:1280400/138‑1 (MQ=255)
                                                 aaaTAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtcct                                                                                           <  1:158847/140‑1 (MQ=255)
                                                 aaaGAACTCTTGGCTTATCAACGACAACTGAGCATCACACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtcct                                                                                           <  1:1353175/140‑1 (MQ=255)
                                                      aCGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGaaa                                                                                                                                    <  1:2156247/94‑1 (MQ=255)
                                                      aCGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGaaa                                                                                                                                    >  2:889979/1‑94 (MQ=255)
                                                      aCGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGaaa                                                                                                                                    <  1:889979/94‑1 (MQ=255)
                                                      aCGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCAt                                                                                      >  1:569549/1‑140 (MQ=255)
                                                       cGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTCGTAGAACTGGTGTTAAACAGTTTCAAGGGTAAACAAGAGCAAATTCCTCCCATg                                                                                     >  1:360355/1‑140 (MQ=255)
                                                           tGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAACCAAAGCCTGTCAATCAAGACAGTGATAAAGCGGGAGTAGAAAAGGTGTTAATCAGTTTAAAGGGTACACAAGAACAAATTCCTCCCATGTAtt                                                                                 >  1:1661484/1‑140 (MQ=255)
                                                           tGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTAtt                                                                                 >  2:62353/1‑140 (MQ=255)
                                                           tGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTAtt                                                                                 >  1:291116/1‑140 (MQ=255)
                                                           tGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTAtt                                                                                 >  1:1318597/1‑140 (MQ=255)
                                                           tGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTAtt                                                                                 >  1:1735548/1‑140 (MQ=255)
                                                            ggCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTc                                                                                <  1:2273289/140‑1 (MQ=255)
                                                            ggCATTTCAACGACAACTGATGATCAAACGGGCTAAACGGTTGAAACAAAGCCTTTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTc                                                                                <  2:2078402/140‑1 (MQ=255)
                                                                  tCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACCTTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGt                                                                          >  1:1870780/1‑140 (MQ=255)
                                                                         cAACTGAGCATCAAACGGGCGAAACGGTTGAAACTAAGCCTGTCAATCAAGACATTGATAACGCGGTAGTAGAATAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAACTTCCTCCCATGTATTCCGCTGTAAAAGTa                                                                   <  2:1658981/140‑1 (MQ=255)
                                                                                 gATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCaa                                                           >  2:1445797/1‑140 (MQ=255)
                                                                                 gATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCaa                                                           >  2:1703167/1‑140 (MQ=255)
                                                                                 gATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCaa                                                           <  1:607168/140‑1 (MQ=255)
                                                                                 gATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCaa                                                           >  2:496222/1‑140 (MQ=255)
                                                                                 gATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCaa                                                           <  1:2270343/140‑1 (MQ=255)
                                                                                    cAAACCGGAGAAACGGTGGAAACAAAGCCTGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCa                                                            >  2:1249608/1‑136 (MQ=255)
                                                                                    cAAACCGGAGAAACGGTGGAAACAAAGCCTGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCa                                                            <  1:1249608/136‑1 (MQ=255)
                                                                                    cAAACCGGAGAAACGGTGGAAACAAAGCCTGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCa                                                            <  1:49753/136‑1 (MQ=255)
                                                                                         gggCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATtat                                                   <  2:656421/140‑1 (MQ=255)
                                                                                             gAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTTATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGa                                               <  2:2043433/140‑1 (MQ=255)
                                                                                             gAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGa                                               <  2:287186/140‑1 (MQ=255)
                                                                                             gAAACGGTTGAAACAAAGCATGTCAATAAAGACATTGATAAAGCGGTAGTAGAAAAGTTGTTAAACAGTTTAAAGGGTAAAAAAGAACAAATTCCTCCCATGTATTCAGCTGTAAAAGTAAACGGCAAAAAATTATACGa                                               <  2:287228/140‑1 (MQ=255)
                                                                                                aCGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTa                                            <  1:284029/140‑1 (MQ=255)
                                                                                                  ggTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACg                                          <  2:1964190/140‑1 (MQ=255)
                                                                                                          cAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCa                                  <  1:400775/140‑1 (MQ=255)
                                                                                                          cAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCa                                  <  1:2218918/140‑1 (MQ=255)
                                                                                                          cAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAAAAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCa                                  <  1:1461947/140‑1 (MQ=255)
                                                                                                             aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGACAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCCGGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAggg                               >  1:815284/1‑140 (MQ=255)
                                                                                                             aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAggg                               >  2:323004/1‑140 (MQ=255)
                                                                                                             aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAggg                               >  2:1529310/1‑140 (MQ=255)
                                                                                                             aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAggg                               >  1:1752251/1‑140 (MQ=255)
                                                                                                             aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAggg                               >  1:2182150/1‑140 (MQ=255)
                                                                                                             aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATAATACGAGTACGCACGAGCAggg                               >  2:795255/1‑140 (MQ=255)
                                                                                                              gCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGa                              <  2:1493648/140‑1 (MQ=255)
                                                                                                              gCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGa                              <  2:1860263/140‑1 (MQ=255)
                                                                                                              gCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGa                              <  2:1747095/140‑1 (MQ=255)
                                                                                                               ccTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGAt                             <  1:1896826/140‑1 (MQ=255)
                                                                                                               ccTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGAt                             <  1:496222/140‑1 (MQ=255)
                                                                                                               ccTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGAt                             <  1:1703167/140‑1 (MQ=255)
                                                                                                                 tGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAATACGCAAGAGCAGGGATTg                           >  2:1249879/1‑140 (MQ=255)
                                                                                                                 tGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAATACGCAAGAGCAGGGATTg                           >  2:2098156/1‑140 (MQ=255)
                                                                                                                  gTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGa                          >  2:957286/1‑140 (MQ=255)
                                                                                                                  gTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGa                          >  2:925529/1‑140 (MQ=255)
                                                                                                                  gTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGa                          >  2:592867/1‑140 (MQ=255)
                                                                                                                  gTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGa                          >  1:724685/1‑140 (MQ=255)
                                                                                                                   tcaatcaaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGaa                         >  1:1412812/1‑140 (MQ=255)
                                                                                                                   tcaatcaaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGaa                         >  2:1023596/1‑140 (MQ=255)
                                                                                                                   tcaatcaaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGaa                         >  1:295359/1‑140 (MQ=255)
                                                                                                                   tcaatcaaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGaa                         >  1:2082564/1‑140 (MQ=255)
                                                                                                                         aaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGaa                   >  2:395535/1‑140 (MQ=255)
                                                                                                                         aaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTACTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGaa                   >  2:1024465/1‑140 (MQ=255)
                                                                                                                                tGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGa            >  2:114294/1‑140 (MQ=255)
                                                                                                                                tGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGa            >  1:189263/1‑140 (MQ=255)
                                                                                                                                tGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGa            >  1:1942867/1‑140 (MQ=255)
                                                                                                                                tGATAAAGCGGTAGTAGAAAAGGTGTGACACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCCAAAAATTATACGAGTACTCAAGAGCAGGGATTGAAGTAGACCGTTCGa            >  2:765654/1‑140 (MQ=255)
                                                                                                                                  aTAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGaaa          <  2:628374/140‑1 (MQ=255)
                                                                                                                                  aTAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGaaa          <  2:1060835/140‑1 (MQ=255)
                                                                                                                                       gCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCAt     <  2:1318597/140‑1 (MQ=255)
                                                                                                                                          gtagtaGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGAt  <  1:2254519/140‑1 (MQ=255)
                                                                                                                                          gtagtaGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGAt  <  2:1752251/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
ACAAAAATTGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGAT  >  NZ_CP034943/1704835‑1705112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: