Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,704,975 A→T 6.0% V100V (GTA→GTT) ‡ truB → tRNA pseudouridine(55) synthase TruB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,704,9750AT 6.0% 370.2 / 9.4 112V100V (GTA→GTT) ‡truBtRNA pseudouridine(55) synthase TruB
Reads supporting (aligned to +/- strand):  ref base A (49/55);  new base T (5/2);  total (55/57)
Fisher's exact test for biased strand distribution p-value = 2.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

TGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTA  >  NZ_CP034943/1704843‑1705114
                                                                                                                                    |                                                                                                                                           
tGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGaaa                                                                                                                                      <  2:1692311/140‑1 (MQ=255)
tGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGaaa                                                                                                                                      <  1:598370/140‑1 (MQ=255)
tGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGaaa                                                                                                                                      <  2:2099599/140‑1 (MQ=255)
          ttAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTaaa                                                                                                                            <  2:31433/140‑1 (MQ=255)
          ttAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTaaa                                                                                                                            <  2:698824/140‑1 (MQ=255)
           tAACTGAAAATTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAAc                                                                                                                           <  2:1825313/140‑1 (MQ=255)
           tAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAACGCGGTAGTAGAAAAGGTGTTAAAc                                                                                                                           <  1:1009875/140‑1 (MQ=255)
           tAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAAc                                                                                                                           <  2:1397327/140‑1 (MQ=255)
           tAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAAc                                                                                                                           <  1:2689/140‑1 (MQ=255)
           tAACTGAAAAGTCTAAAACGTATGAGGCGGAAATAACGCTTGGCTTTTCAACGACAACTGCGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAAc                                                                                                                           <  2:1274948/140‑1 (MQ=255)
           tAACTGAAAAGTCGAAAACGGCTGATGCGGAAATAACGCTTGGCTTTTCAACTACAACGGACGATCAAACGGGCGAAACCGTTGAAACAAAGCCTGTCAATCACGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAAc                                                                                                                           <  1:1132203/140‑1 (MQ=255)
            aaCTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACa                                                                                                                          >  1:2286735/1‑140 (MQ=255)
                 aaaaGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTa                                                                                                                     >  2:860171/1‑140 (MQ=255)
                 aaaaGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTa                                                                                                                     <  2:1843283/140‑1 (MQ=255)
                    aGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAg                                                                                                                  >  2:2270343/1‑140 (MQ=255)
                    aGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAg                                                                                                                  >  2:607168/1‑140 (MQ=255)
                            aCGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACa                                                                                                          <  2:710580/140‑1 (MQ=255)
                            aCGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACa                                                                                                          <  2:1351461/140‑1 (MQ=255)
                                       ggAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtc                                                                                               <  2:1670312/140‑1 (MQ=255)
                                       ggAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtc                                                                                               <  2:387829/140‑1 (MQ=255)
                                       ggAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtc                                                                                               <  2:1767522/140‑1 (MQ=255)
                                        gAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTATAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtcc                                                                                              >  1:2078402/1‑140 (MQ=255)
                                        gAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtcc                                                                                              >  2:2355822/1‑140 (MQ=255)
                                        gAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtcc                                                                                              >  2:2292654/1‑140 (MQ=255)
                                        gAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtcc                                                                                              >  1:1297138/1‑140 (MQ=255)
                                        gAAATAACGCTGGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACACAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTCAACAAGTACAAATacc                                                                                              >  1:1586640/1‑140 (MQ=255)
                                         ataTAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTCAAGGGTAAACAAGAACAAATtcct                                                                                             <  1:1280400/138‑1 (MQ=255)
                                         aaaTAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtcct                                                                                             <  1:158847/140‑1 (MQ=255)
                                         aaaGAACTCTTGGCTTATCAACGACAACTGAGCATCACACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtcct                                                                                             <  1:1353175/140‑1 (MQ=255)
                                            tAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTccc                                                                                          <  2:670791/140‑1 (MQ=255)
                                              aCGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGaaa                                                                                                                                      <  1:2156247/94‑1 (MQ=255)
                                              aCGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGaaa                                                                                                                                      <  1:889979/94‑1 (MQ=255)
                                              aCGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGaaa                                                                                                                                      >  2:2156247/1‑94 (MQ=255)
                                              aCGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGaaa                                                                                                                                      >  2:889979/1‑94 (MQ=255)
                                              aCGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCAt                                                                                        >  1:569549/1‑140 (MQ=255)
                                               cGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTCGTAGAACTGGTGTTAAACAGTTTCAAGGGTAAACAAGAGCAAATTCCTCCCATg                                                                                       >  1:360355/1‑140 (MQ=255)
                                                   tGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAACCAAAGCCTGTCAATCAAGACAGTGATAAAGCGGGAGTAGAAAAGGTGTTAATCAGTTTAAAGGGTACACAAGAACAAATTCCTCCCATGTAtt                                                                                   >  1:1661484/1‑140 (MQ=255)
                                                   tGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTAtt                                                                                   >  1:1735548/1‑140 (MQ=255)
                                                   tGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTAtt                                                                                   >  1:291116/1‑140 (MQ=255)
                                                   tGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTAtt                                                                                   >  1:1860263/1‑140 (MQ=255)
                                                   tGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTAtt                                                                                   >  2:62353/1‑140 (MQ=255)
                                                    ggCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTc                                                                                  <  1:2273289/140‑1 (MQ=255)
                                                    ggCATTTCAACGACAACTGATGATCAAACGGGCTAAACGGTTGAAACAAAGCCTTTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTc                                                                                  <  2:2078402/140‑1 (MQ=255)
                                                         ttCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAAACCAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAAGTCCTCCCATGTATTCCGCTg                                                                             <  1:2241367/140‑1 (MQ=255)
                                                          tCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACCTTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGt                                                                            >  1:1870780/1‑140 (MQ=255)
                                                                 cAACTGAGCATCAAACGGGCGAAACGGTTGAAACTAAGCCTGTCAATCAAGACATTGATAACGCGGTAGTAGAATAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAACTTCCTCCCATGTATTCCGCTGTAAAAGTa                                                                     <  2:1658981/140‑1 (MQ=255)
                                                                         gATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCaa                                                             >  2:496222/1‑140 (MQ=255)
                                                                         gATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCaa                                                             <  1:2270343/140‑1 (MQ=255)
                                                                            cAAACCGGAGAAACGGTGGAAACAAAGCCTGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCa                                                              >  2:1249608/1‑136 (MQ=255)
                                                                            cAAACCGGAGAAACGGTGGAAACAAAGCCTGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCa                                                              <  1:1249608/136‑1 (MQ=255)
                                                                            cAAACCGGAGAAACGGTGGAAACAAAGCCTGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCa                                                              >  2:49753/1‑136 (MQ=255)
                                                                            cAAACCGGAGAAACGGTGGAAACAAAGCCTGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCa                                                              <  1:49753/136‑1 (MQ=255)
                                                                                 gggCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATtat                                                     <  2:656421/140‑1 (MQ=255)
                                                                                     gAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTTATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGa                                                 <  2:2043433/140‑1 (MQ=255)
                                                                                     gAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGa                                                 <  2:287186/140‑1 (MQ=255)
                                                                                     gAAACGGTTGAAACAAAGCATGTCAATAAAGACATTGATAAAGCGGTAGTAGAAAAGTTGTTAAACAGTTTAAAGGGTAAAAAAGAACAAATTCCTCCCATGTATTCAGCTGTAAAAGTAAACGGCAAAAAATTATACGa                                                 <  2:287228/140‑1 (MQ=255)
                                                                                        aCGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTa                                              <  1:284029/140‑1 (MQ=255)
                                                                                          ggTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACg                                            <  2:1964190/140‑1 (MQ=255)
                                                                                           gTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCCTGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGc                                           <  2:315434/140‑1 (MQ=255)
                                                                                                  cAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCa                                    <  1:2218918/140‑1 (MQ=255)
                                                                                                  cAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCa                                    <  1:400775/140‑1 (MQ=255)
                                                                                                  cAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAAAAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCa                                    <  1:1461947/140‑1 (MQ=255)
                                                                                                     aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGACAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCCGGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAggg                                 >  1:815284/1‑140 (MQ=255)
                                                                                                     aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAggg                                 >  2:1529310/1‑140 (MQ=255)
                                                                                                     aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAggg                                 >  1:1752251/1‑140 (MQ=255)
                                                                                                     aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAggg                                 >  2:323004/1‑140 (MQ=255)
                                                                                                     aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAggg                                 >  1:2182150/1‑140 (MQ=255)
                                                                                                     aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAggg                                 >  2:2004007/1‑140 (MQ=255)
                                                                                                     aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATAATACGAGTACGCACGAGCAggg                                 >  2:795255/1‑140 (MQ=255)
                                                                                                      gCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGa                                <  2:1747095/140‑1 (MQ=255)
                                                                                                      gCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGa                                <  2:1860263/140‑1 (MQ=255)
                                                                                                      gCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGa                                <  2:1493648/140‑1 (MQ=255)
                                                                                                       ccTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGAt                               <  1:496222/140‑1 (MQ=255)
                                                                                                       ccTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGAt                               <  1:990313/140‑1 (MQ=255)
                                                                                                       ccTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGAt                               <  1:1896826/140‑1 (MQ=255)
                                                                                                       ccTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGAt                               <  1:1445797/140‑1 (MQ=255)
                                                                                                         tGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAATACGCAAGAGCAGGGATTg                             >  2:2098156/1‑140 (MQ=255)
                                                                                                         tGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAATACGCAAGAGCAGGGATTg                             >  2:1249879/1‑140 (MQ=255)
                                                                                                         tGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAATACGCAAGAGCAGGGATTg                             >  2:2385081/1‑140 (MQ=255)
                                                                                                          gTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGa                            >  2:795812/1‑140 (MQ=255)
                                                                                                          gTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGa                            >  2:925529/1‑140 (MQ=255)
                                                                                                          gTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGa                            >  2:957286/1‑140 (MQ=255)
                                                                                                          gTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGa                            >  1:724685/1‑140 (MQ=255)
                                                                                                           tcaatcaaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGaa                           >  1:1305249/1‑140 (MQ=255)
                                                                                                           tcaatcaaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGaa                           >  1:1412812/1‑140 (MQ=255)
                                                                                                           tcaatcaaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGaa                           >  1:2052236/1‑140 (MQ=255)
                                                                                                           tcaatcaaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGaa                           >  1:2082564/1‑140 (MQ=255)
                                                                                                           tcaatcaaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGaa                           >  1:295359/1‑140 (MQ=255)
                                                                                                           tcaatcaaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGaa                           >  2:1023596/1‑140 (MQ=255)
                                                                                                                 aaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGaa                     >  2:395535/1‑140 (MQ=255)
                                                                                                                 aaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTACTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGaa                     >  2:1024465/1‑140 (MQ=255)
                                                                                                                     cATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGtt                 <  2:1826536/140‑1 (MQ=255)
                                                                                                                        tGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGa              >  2:114294/1‑140 (MQ=255)
                                                                                                                        tGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGa              >  1:189263/1‑140 (MQ=255)
                                                                                                                          aTAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGaaa            <  2:628374/140‑1 (MQ=255)
                                                                                                                          aTAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGaaa            <  2:1060835/140‑1 (MQ=255)
                                                                                                                           tAAAGCGTTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAAc           <  2:1468070/140‑1 (MQ=255)
                                                                                                                               gCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCAt       <  2:1318597/140‑1 (MQ=255)
                                                                                                                                  gtagtaGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGAt    <  2:1752251/140‑1 (MQ=255)
                                                                                                                                    agtagAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTa  <  1:1023596/140‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                           
TGTCGAATACTTAACTGAAAAGTCTAAAACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTA  >  NZ_CP034943/1704843‑1705114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: