Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,705,005 T→G 6.7% G110G (GGT→GGG truB → tRNA pseudouridine(55) synthase TruB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,705,0050TG 6.7% 332.1 / 9.7 105G110G (GGT→GGGtruBtRNA pseudouridine(55) synthase TruB
Reads supporting (aligned to +/- strand):  ref base T (52/46);  new base G (5/2);  total (57/48)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 105 total aligned reads displayed.

ACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACATCGCCCTGA  >  NZ_CP034943/1704871‑1705135
                                                                                                                                      |                                                                                                                                  
aCGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACa                                                                                                                               <  2:1351461/140‑1 (MQ=255)
aCGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACa                                                                                                                               <  2:710580/140‑1 (MQ=255)
           ggAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtc                                                                                                                    <  2:1670312/140‑1 (MQ=255)
           ggAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtc                                                                                                                    <  2:1767522/140‑1 (MQ=255)
           ggAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtc                                                                                                                    <  2:387829/140‑1 (MQ=255)
            gAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTATAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtcc                                                                                                                   >  1:2078402/1‑140 (MQ=255)
            gAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtcc                                                                                                                   >  2:2355822/1‑140 (MQ=255)
            gAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtcc                                                                                                                   >  1:1297138/1‑140 (MQ=255)
            gAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtcc                                                                                                                   >  2:2292654/1‑140 (MQ=255)
            gAAATAACGCTGGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACACAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTCAACAAGTACAAATacc                                                                                                                   >  1:1586640/1‑140 (MQ=255)
             ataTAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTCAAGGGTAAACAAGAACAAATtcct                                                                                                                  <  1:1280400/138‑1 (MQ=255)
             aaaTAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtcct                                                                                                                  <  1:158847/140‑1 (MQ=255)
             aaaGAACTCTTGGCTTATCAACGACAACTGAGCATCACACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtcct                                                                                                                  <  1:1353175/140‑1 (MQ=255)
                tAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTccc                                                                                                               <  2:670791/140‑1 (MQ=255)
                   cGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTCGTAGAACTGGTGTTAAACAGTTTCAAGGGTAAACAAGAGCAAATTCCTCCCATg                                                                                                            >  1:360355/1‑140 (MQ=255)
                       tGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAACCAAAGCCTGTCAATCAAGACAGTGATAAAGCGGGAGTAGAAAAGGTGTTAATCAGTTTAAAGGGTACACAAGAACAAATTCCTCCCATGTAtt                                                                                                        >  1:1661484/1‑140 (MQ=255)
                       tGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTAtt                                                                                                        >  1:1735548/1‑140 (MQ=255)
                       tGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTAtt                                                                                                        >  1:1860263/1‑140 (MQ=255)
                       tGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTAtt                                                                                                        >  1:291116/1‑140 (MQ=255)
                       tGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTAtt                                                                                                        >  1:1318597/1‑140 (MQ=255)
                       tGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTAtt                                                                                                        >  2:62353/1‑140 (MQ=255)
                        ggCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTc                                                                                                       <  1:2273289/140‑1 (MQ=255)
                        ggCATTTCAACGACAACTGATGATCAAACGGGCTAAACGGTTGAAACAAAGCCTTTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTc                                                                                                       <  2:2078402/140‑1 (MQ=255)
                             ttCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAAACCAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAAGTCCTCCCATGTATTCCGCTg                                                                                                  <  1:2241367/140‑1 (MQ=255)
                              tCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACCTTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGt                                                                                                 >  1:1870780/1‑140 (MQ=255)
                                     cAACTGAGCATCAAACGGGCGAAACGGTTGAAACTAAGCCTGTCAATCAAGACATTGATAACGCGGTAGTAGAATAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAACTTCCTCCCATGTATTCCGCTGTAAAAGTa                                                                                          <  2:1658981/140‑1 (MQ=255)
                                             gATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCaa                                                                                  >  2:496222/1‑140 (MQ=255)
                                             gATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCaa                                                                                  <  1:2270343/140‑1 (MQ=255)
                                             gATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCaa                                                                                  <  1:607168/140‑1 (MQ=255)
                                             gATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCaa                                                                                  >  2:1445797/1‑140 (MQ=255)
                                             gATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCaa                                                                                  >  2:1703167/1‑140 (MQ=255)
                                                cAAACCGGAGAAACGGTGGAAACAAAGCCTGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCa                                                                                   <  1:49753/136‑1 (MQ=255)
                                                cAAACCGGAGAAACGGTGGAAACAAAGCCTGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCa                                                                                   >  2:1249608/1‑136 (MQ=255)
                                                cAAACCGGAGAAACGGTGGAAACAAAGCCTGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCa                                                                                   <  1:1249608/136‑1 (MQ=255)
                                                cAAACCGGAGAAACGGTGGAAACAAAGCCTGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCa                                                                                   >  2:49753/1‑136 (MQ=255)
                                                     gggCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATtat                                                                          <  2:656421/140‑1 (MQ=255)
                                                         gAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTTATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGa                                                                      <  2:2043433/140‑1 (MQ=255)
                                                         gAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGa                                                                      <  2:287186/140‑1 (MQ=255)
                                                            aCGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTa                                                                   <  1:284029/140‑1 (MQ=255)
                                                              ggTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACg                                                                 <  2:1964190/140‑1 (MQ=255)
                                                               gTTGAAACAAAGCCTGTCAATCTAGCCATTGATTAAGCGGGAGTAGAAAAGGTGTTTAACAGTTTAAAGGTTATCCATTAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTTAAGGGCTAAAAAGTATACGAGTACGc                                                                <  2:1114208/140‑1 (MQ=255)
                                                               gTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCCTGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGc                                                                <  2:315434/140‑1 (MQ=255)
                                                                      cAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCa                                                         <  1:400775/140‑1 (MQ=255)
                                                                      cAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCa                                                         <  1:2218918/140‑1 (MQ=255)
                                                                      cAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAAAAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCa                                                         <  1:1461947/140‑1 (MQ=255)
                                                                         aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGACAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCCGGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAggg                                                      >  1:815284/1‑140 (MQ=255)
                                                                         aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAggg                                                      >  1:2182150/1‑140 (MQ=255)
                                                                         aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAggg                                                      >  1:1752251/1‑140 (MQ=255)
                                                                         aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAggg                                                      >  2:323004/1‑140 (MQ=255)
                                                                         aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAggg                                                      >  2:2004007/1‑140 (MQ=255)
                                                                         aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATAATACGAGTACGCACGAGCAggg                                                      >  2:795255/1‑140 (MQ=255)
                                                                          gCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGa                                                     <  2:1747095/140‑1 (MQ=255)
                                                                          gCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGa                                                     <  2:1493648/140‑1 (MQ=255)
                                                                          gCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGa                                                     <  2:1860263/140‑1 (MQ=255)
                                                                           ccTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGAt                                                    <  1:496222/140‑1 (MQ=255)
                                                                           ccTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGAt                                                    <  1:1703167/140‑1 (MQ=255)
                                                                           ccTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGAt                                                    <  1:990313/140‑1 (MQ=255)
                                                                           ccTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGAt                                                    <  1:1445797/140‑1 (MQ=255)
                                                                             tGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAATACGCAAGAGCAGGGATTg                                                  >  2:1249879/1‑140 (MQ=255)
                                                                             tGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAATACGCAAGAGCAGGGATTg                                                  >  2:2098156/1‑140 (MQ=255)
                                                                             tGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAATACGCAAGAGCAGGGATTg                                                  >  2:2385081/1‑140 (MQ=255)
                                                                              gTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGa                                                 >  2:592867/1‑140 (MQ=255)
                                                                              gTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGa                                                 >  2:795812/1‑140 (MQ=255)
                                                                              gTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGa                                                 >  2:925529/1‑140 (MQ=255)
                                                                              gTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGa                                                 >  2:957286/1‑140 (MQ=255)
                                                                              gTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGa                                                 >  1:724685/1‑140 (MQ=255)
                                                                               tcaatcaaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGaa                                                >  1:2082564/1‑140 (MQ=255)
                                                                               tcaatcaaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGaa                                                >  1:295359/1‑140 (MQ=255)
                                                                               tcaatcaaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGaa                                                >  1:1305249/1‑140 (MQ=255)
                                                                               tcaatcaaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGaa                                                >  1:2052236/1‑140 (MQ=255)
                                                                               tcaatcaaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGaa                                                >  1:1412812/1‑140 (MQ=255)
                                                                               tcaatcaaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGaa                                                >  2:1023596/1‑140 (MQ=255)
                                                                                     aaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGaa                                          >  2:395535/1‑140 (MQ=255)
                                                                                     aaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTACTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGaa                                          >  2:1024465/1‑140 (MQ=255)
                                                                                         cATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGtt                                      <  2:1826536/140‑1 (MQ=255)
                                                                                            tGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGa                                   >  2:114294/1‑140 (MQ=255)
                                                                                            tGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGa                                   >  1:1942867/1‑140 (MQ=255)
                                                                                            tGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGa                                   >  1:189263/1‑140 (MQ=255)
                                                                                            tGATAAAGCGGTAGTAGAAAAGGTGTGACACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCCAAAAATTATACGAGTACTCAAGAGCAGGGATTGAAGTAGACCGTTCGa                                   >  2:765654/1‑140 (MQ=255)
                                                                                              aTAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGaaa                                 <  2:628374/140‑1 (MQ=255)
                                                                                              aTAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGaaa                                 <  2:1060835/140‑1 (MQ=255)
                                                                                               tAAAGCGTTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAAc                                <  2:1468070/140‑1 (MQ=255)
                                                                                                aaaGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACg                               <  1:312006/140‑1 (MQ=255)
                                                                                                   gCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCAt                            <  2:1318597/140‑1 (MQ=255)
                                                                                                      gtagtaGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGAt                         <  2:1752251/140‑1 (MQ=255)
                                                                                                      gtagtaGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGAt                         <  1:2254519/140‑1 (MQ=255)
                                                                                                        agtagAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTa                       <  1:1023596/140‑1 (MQ=255)
                                                                                                              aaaGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCACAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGCTTACCATTg                 >  2:1365387/1‑140 (MQ=255)
                                                                                                               aaGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGa                <  2:41925/140‑1 (MQ=255)
                                                                                                                    gtTAAACAGTTTAAAGGGTAAACAAGACAAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACa           <  2:2214891/140‑1 (MQ=255)
                                                                                                                    gtTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACa           >  1:458333/1‑140 (MQ=255)
                                                                                                                    gtTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACa           >  1:458361/1‑140 (MQ=255)
                                                                                                                    gtTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACa           >  1:691597/1‑140 (MQ=255)
                                                                                                                    gtTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACa           >  1:876144/1‑140 (MQ=255)
                                                                                                                    gtTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACa           >  2:2233728/1‑140 (MQ=255)
                                                                                                                    gtTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACa           >  2:1568436/1‑140 (MQ=255)
                                                                                                                     ttAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACAt          >  1:1572110/1‑140 (MQ=255)
                                                                                                                         aCAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACATCGcc      <  2:724685/140‑1 (MQ=255)
                                                                                                                             tttAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCACGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCCTTGAAGACATCGCCCTga  >  2:1380987/1‑140 (MQ=255)
                                                                                                                             tttAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACATCGCCCTga  >  2:41577/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                  
ACGTATGATGCGGAAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACATCGCCCTGA  >  NZ_CP034943/1704871‑1705135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: