Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,705,023 T→G 6.8% P116P (CCT→CCG truB → tRNA pseudouridine(55) synthase TruB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,705,0230TG 6.8% 311.8 / 9.8 103P116P (CCT→CCGtruBtRNA pseudouridine(55) synthase TruB
Reads supporting (aligned to +/- strand):  ref base T (54/42);  new base G (5/2);  total (59/44)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01

Only 100 of 107 total aligned reads displayed.

AAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACATCGCCCTGACGACAGAGATTAAACTTAACGGAGAAA  >  NZ_CP034943/1704884‑1705162
                                                                                                                                           |                                                                                                                                           
ataTAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTCAAGGGTAAACAAGAACAAATtcct                                                                                                                                             <  1:1280400/138‑1 (MQ=255)
aaaTAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtcct                                                                                                                                             <  1:158847/140‑1 (MQ=255)
aaaGAACTCTTGGCTTATCAACGACAACTGAGCATCACACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATtcct                                                                                                                                             <  1:1353175/140‑1 (MQ=255)
   tAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTccc                                                                                                                                          <  2:670791/140‑1 (MQ=255)
     aCGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCAt                                                                                                                                        >  1:569549/1‑140 (MQ=255)
      cGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTCGTAGAACTGGTGTTAAACAGTTTCAAGGGTAAACAAGAGCAAATTCCTCCCATg                                                                                                                                       >  1:360355/1‑140 (MQ=255)
          tGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAACCAAAGCCTGTCAATCAAGACAGTGATAAAGCGGGAGTAGAAAAGGTGTTAATCAGTTTAAAGGGTACACAAGAACAAATTCCTCCCATGTAtt                                                                                                                                   >  1:1661484/1‑140 (MQ=255)
          tGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTAtt                                                                                                                                   >  1:291116/1‑140 (MQ=255)
          tGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTAtt                                                                                                                                   >  2:62353/1‑140 (MQ=255)
          tGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTAtt                                                                                                                                   >  1:1318597/1‑140 (MQ=255)
          tGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTAtt                                                                                                                                   >  1:1860263/1‑140 (MQ=255)
          tGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTAtt                                                                                                                                   >  1:1735548/1‑140 (MQ=255)
           ggCATTTCAACGACAACTGATGATCAAACGGGCTAAACGGTTGAAACAAAGCCTTTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTc                                                                                                                                  <  2:2078402/140‑1 (MQ=255)
                ttCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAAACCAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAAGTCCTCCCATGTATTCCGCTg                                                                                                                             <  1:2241367/140‑1 (MQ=255)
                 tCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACCTTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGt                                                                                                                            >  1:1870780/1‑140 (MQ=255)
                        cAACTGAGCATCAAACGGGCGAAACGGTTGAAACTAAGCCTGTCAATCAAGACATTGATAACGCGGTAGTAGAATAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAACTTCCTCCCATGTATTCCGCTGTAAAAGTa                                                                                                                     <  2:1658981/140‑1 (MQ=255)
                                gATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCaa                                                                                                             <  1:607168/140‑1 (MQ=255)
                                gATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCaa                                                                                                             >  2:1445797/1‑140 (MQ=255)
                                gATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCaa                                                                                                             <  1:2270343/140‑1 (MQ=255)
                                gATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCaa                                                                                                             >  2:1703167/1‑140 (MQ=255)
                                   cAAACCGGAGAAACGGTGGAAACAAAGCCTGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCa                                                                                                              <  1:1249608/136‑1 (MQ=255)
                                   cAAACCGGAGAAACGGTGGAAACAAAGCCTGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCa                                                                                                              >  2:1249608/1‑136 (MQ=255)
                                   cAAACCGGAGAAACGGTGGAAACAAAGCCTGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCa                                                                                                              <  1:49753/136‑1 (MQ=255)
                                   cAAACCGGAGAAACGGTGGAAACAAAGCCTGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCa                                                                                                              >  2:49753/1‑136 (MQ=255)
                                        gggCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATtat                                                                                                     <  2:656421/140‑1 (MQ=255)
                                            gAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTTATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGa                                                                                                 <  2:2043433/140‑1 (MQ=255)
                                            gAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGa                                                                                                 <  2:287186/140‑1 (MQ=255)
                                            gAAACGGTTGAAACAAAGCATGTCAATAAAGACATTGATAAAGCGGTAGTAGAAAAGTTGTTAAACAGTTTAAAGGGTAAAAAAGAACAAATTCCTCCCATGTATTCAGCTGTAAAAGTAAACGGCAAAAAATTATACGa                                                                                                 <  2:287228/140‑1 (MQ=255)
                                               aCGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTa                                                                                              <  1:284029/140‑1 (MQ=255)
                                                  gTTGAAACAAAGCCTGTCAATCTAGCCATTGATTAAGCGGGAGTAGAAAAGGTGTTTAACAGTTTAAAGGTTATCCATTAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTTAAGGGCTAAAAAGTATACGAGTACGc                                                                                           <  2:1114208/140‑1 (MQ=255)
                                                  gTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCCTGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGc                                                                                           <  2:315434/140‑1 (MQ=255)
                                                         cAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCa                                                                                    <  1:400775/140‑1 (MQ=255)
                                                         cAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCa                                                                                    <  1:2218918/140‑1 (MQ=255)
                                                         cAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAAAAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCa                                                                                    <  1:1461947/140‑1 (MQ=255)
                                                            aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAggg                                                                                 >  1:2182150/1‑140 (MQ=255)
                                                            aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAggg                                                                                 >  1:1752251/1‑140 (MQ=255)
                                                            aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAggg                                                                                 >  2:323004/1‑140 (MQ=255)
                                                            aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAggg                                                                                 >  2:2004007/1‑140 (MQ=255)
                                                            aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAggg                                                                                 >  2:1529310/1‑140 (MQ=255)
                                                            aGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATAATACGAGTACGCACGAGCAggg                                                                                 >  2:795255/1‑140 (MQ=255)
                                                             gCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGa                                                                                <  2:1860263/140‑1 (MQ=255)
                                                             gCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGa                                                                                <  2:1493648/140‑1 (MQ=255)
                                                             gCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGa                                                                                <  2:1747095/140‑1 (MQ=255)
                                                              ccTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGAt                                                                               <  1:496222/140‑1 (MQ=255)
                                                              ccTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGAt                                                                               <  1:1896826/140‑1 (MQ=255)
                                                              ccTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGAt                                                                               <  1:990313/140‑1 (MQ=255)
                                                              ccTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGAt                                                                               <  1:1445797/140‑1 (MQ=255)
                                                                tGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAATACGCAAGAGCAGGGATTg                                                                             >  2:1249879/1‑140 (MQ=255)
                                                                tGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAATACGCAAGAGCAGGGATTg                                                                             >  2:2385081/1‑140 (MQ=255)
                                                                tGTCAATCACGACATTGATAAAGCGGATGTAGAAAAGGTTTTAAACAGTTTAAAGGGGAAACAAGAACAAATTCCGCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAATACGCAAGAGCAGGGATTg                                                                             >  2:2098156/1‑140 (MQ=255)
                                                                 gTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGa                                                                            >  2:592867/1‑140 (MQ=255)
                                                                 gTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGa                                                                            >  1:724685/1‑140 (MQ=255)
                                                                 gTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGa                                                                            >  2:795812/1‑140 (MQ=255)
                                                                 gTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGa                                                                            >  2:925529/1‑140 (MQ=255)
                                                                 gTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGa                                                                            >  2:957286/1‑140 (MQ=255)
                                                                  tcaatcaaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGaa                                                                           >  2:1023596/1‑140 (MQ=255)
                                                                  tcaatcaaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGaa                                                                           >  1:295359/1‑140 (MQ=255)
                                                                  tcaatcaaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGaa                                                                           >  1:2082564/1‑140 (MQ=255)
                                                                  tcaatcaaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGaa                                                                           >  1:2052236/1‑140 (MQ=255)
                                                                  tcaatcaaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGaa                                                                           >  1:1412812/1‑140 (MQ=255)
                                                                  tcaatcaaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGaa                                                                           >  1:1305249/1‑140 (MQ=255)
                                                                        aaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGaa                                                                     >  2:395535/1‑140 (MQ=255)
                                                                        aaGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTACTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGaa                                                                     >  2:1024465/1‑140 (MQ=255)
                                                                            cATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGtt                                                                 <  2:1826536/140‑1 (MQ=255)
                                                                               tGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGa                                                              >  2:114294/1‑140 (MQ=255)
                                                                               tGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGa                                                              >  1:1942867/1‑140 (MQ=255)
                                                                               tGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGa                                                              >  1:189263/1‑140 (MQ=255)
                                                                               tGATAAAGCGGTAGTAGAAAAGGTGTGACACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCCAAAAATTATACGAGTACTCAAGAGCAGGGATTGAAGTAGACCGTTCGa                                                              >  2:765654/1‑140 (MQ=255)
                                                                                 aTAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGaaa                                                            <  2:628374/140‑1 (MQ=255)
                                                                                 aTAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGaaa                                                            <  2:1060835/140‑1 (MQ=255)
                                                                                  tAAAGCGTTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAAc                                                           <  2:1468070/140‑1 (MQ=255)
                                                                                   aaaGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACg                                                          <  1:312006/140‑1 (MQ=255)
                                                                                      gCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCAt                                                       <  2:1318597/140‑1 (MQ=255)
                                                                                         gtagtaGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGAt                                                    <  2:1752251/140‑1 (MQ=255)
                                                                                         gtagtaGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGAt                                                    <  1:2254519/140‑1 (MQ=255)
                                                                                           agtagAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTa                                                  <  1:1023596/140‑1 (MQ=255)
                                                                                                 aaaGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCACAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGCTTACCATTg                                            >  2:1365387/1‑140 (MQ=255)
                                                                                                  aaGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGa                                           <  2:41925/140‑1 (MQ=255)
                                                                                                       gtTAAACAGTTTAAAGGGTAAACAAGACAAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACa                                      <  2:2214891/140‑1 (MQ=255)
                                                                                                       gtTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACa                                      >  2:2233728/1‑140 (MQ=255)
                                                                                                       gtTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACa                                      >  1:458333/1‑140 (MQ=255)
                                                                                                       gtTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACa                                      >  1:458361/1‑140 (MQ=255)
                                                                                                       gtTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACa                                      >  1:691597/1‑140 (MQ=255)
                                                                                                       gtTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACa                                      >  1:876144/1‑140 (MQ=255)
                                                                                                       gtTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACa                                      >  2:1568436/1‑140 (MQ=255)
                                                                                                        ttAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACAt                                     >  1:1572110/1‑140 (MQ=255)
                                                                                                            aCAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACATCGcc                                 <  2:724685/140‑1 (MQ=255)
                                                                                                                tttAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCACGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCCTTGAAGACATCGCCCTga                             >  2:1380987/1‑140 (MQ=255)
                                                                                                                tttAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACATCGCCCTga                             >  2:41577/1‑140 (MQ=255)
                                                                                                                          aaaCAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGa                                                                                <  1:1177120/79‑1 (MQ=255)
                                                                                                                          aaaCAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGa                                                                                >  2:1177120/1‑79 (MQ=255)
                                                                                                                           aaCAAGAACAACTTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTAGACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACATCGCCCTGACGACAGAGAtt                  <  2:1586640/140‑1 (MQ=255)
                                                                                                                           aaCAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACATCGCCCTGACGACAGAGAtt                  <  2:1297138/140‑1 (MQ=255)
                                                                                                                                 aaaaaaTTCATCCCATGTATTCCGCTGAAAAAGTAAACGCCAAAAAATTATACGAGTACGCAAGAGCAGGGATTTAAGAAGAACGTTCTAAACGCATGATAACCATTGAAGACAGCGCCATGACCACATAGATaaaaata            >  2:898125/4‑137 (MQ=255)
                                                                                                                                 aaCAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGAt                                                    >  1:1403544/1‑100 (MQ=255)
                                                                                                                                 aaCAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAATACATCGCCCTGACGACAGAGATTAAACtt            >  1:1608660/1‑140 (MQ=255)
                                                                                                                                 aaCAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACATCGCCCTGACGACAGAGATTAAACtt            >  1:909285/1‑140 (MQ=255)
                                                                                                                                 aaCAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACATCGCCCTGACGACAGAGATTAAACtt            >  2:1745946/1‑140 (MQ=255)
                                                                                                                                 aaCAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTAAACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCAGTGAAGACATCGCCCTGACGACCGAGATGAAACtt            >  2:1690513/1‑140 (MQ=255)
                                                                                                                                           tccCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACATCGCCCTGACGACAGAGATTAAACTTAACGGAGaaa  >  2:671658/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AAATAACGCTTGGCTTTTCAACGACAACTGAGGATCAAACGGGCGAAACGGTTGAAACAAAGCCTGTCAATCAAGACATTGATAAAGCGGTAGTAGAAAAGGTGTTAAACAGTTTAAAGGGTAAACAAGAACAAATTCCTCCCATGTATTCCGCTGTAAAAGTAAACGGCAAAAAATTATACGAGTACGCAAGAGCAGGGATTGAAGTAGAACGTTCGAAACGCATGATTACCATTGAAGACATCGCCCTGACGACAGAGATTAAACTTAACGGAGAAA  >  NZ_CP034943/1704884‑1705162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: