Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,734,397 T→C 8.8% N221N (AAT→AAC EO946_RS09000 → serine hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,734,3970TC 8.8% 471.4 / 25.8 158N221N (AAT→AACEO946_RS09000serine hydrolase
Reads supporting (aligned to +/- strand):  ref base T (69/75);  new base C (7/7);  total (76/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

Only 100 of 162 total aligned reads displayed.

TTACACATACATCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTG  >  NZ_CP034943/1734267‑1734533
                                                                                                                                  |                                                                                                                                        
ttACACATACATCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGtt                                                                                                                                 <  1:1121956/140‑1 (MQ=255)
ttACACATACATCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGtt                                                                                                                                 <  2:71310/140‑1 (MQ=255)
ttACACATACATCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGtt                                                                                                                                 <  1:1497674/140‑1 (MQ=255)
ttACACATACATCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGtt                                                                                                                                 <  1:2176673/140‑1 (MQ=255)
ttACACATACATCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGtt                                                                                                                                 <  2:2178407/140‑1 (MQ=255)
ttACACATACATCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGtt                                                                                                                                 <  1:2053208/140‑1 (MQ=255)
ttACACATACATCGGGAATAAAAGTTCATATTTAAGTAAACGGAGCAATCACTCCGTACGATTTAATAAAAGACGTAGAAAATAAAGGAATCAAACGTCAGCCGGTTTTATGGGAATATAAGGATTCCAATTATTCTGtt                                                                                                                                 <  2:2294366/140‑1 (MQ=255)
  acacATACATCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTtct                                                                                                                               <  2:1976623/140‑1 (MQ=255)
  acacATACATCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTtct                                                                                                                               <  2:1566342/140‑1 (MQ=255)
  acacATACATCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTtct                                                                                                                               <  2:105189/140‑1 (MQ=255)
        acatCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAATGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTta                                                                                                                         >  1:2133366/1‑140 (MQ=255)
        acatCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAACCGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTta                                                                                                                         <  1:508477/140‑1 (MQ=255)
         catCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCttct                                                                                                                        >  2:2064685/1‑138 (MQ=255)
         catCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTtat                                                                                                                        >  2:1261118/1‑140 (MQ=255)
         catCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTtat                                                                                                                        >  1:2312206/1‑140 (MQ=255)
         catCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTtat                                                                                                                        >  1:2401517/1‑140 (MQ=255)
         catCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAAACACTCCGGACGATTGAATAAAAGACGTAGAACATCAACTAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCtgag                                                                                                                        >  1:319426/1‑137 (MQ=255)
           tCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTatat                                                                                                                      <  2:1848256/140‑1 (MQ=255)
           tCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTatat                                                                                                                      <  2:728356/140‑1 (MQ=255)
            cGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATAtt                                                                                                                     >  2:202359/1‑140 (MQ=255)
            cGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATAtt                                                                                                                     <  2:1285118/140‑1 (MQ=255)
            cGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATAtt                                                                                                                     <  2:278437/140‑1 (MQ=255)
              ggAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGt                                                                                                                   >  1:2231377/1‑140 (MQ=255)
              ggAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATGGt                                                                                                                   >  2:388052/1‑140 (MQ=255)
                  tAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGTACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCt                                                                                                               <  1:91577/140‑1 (MQ=255)
                         tCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTaa                                                                                                        >  1:458943/1‑140 (MQ=255)
                              ttGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGt                                                                                                   <  1:202359/140‑1 (MQ=255)
                              ttGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGt                                                                                                   <  2:2231377/140‑1 (MQ=255)
                              ttGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGt                                                                                                   <  2:418251/140‑1 (MQ=255)
                                gcaGGCAACGGCGCAATCACTCCGGACGAGTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGa                                                                                                 <  1:1879013/138‑1 (MQ=255)
                                gAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGa                                                                                                 <  1:1194829/140‑1 (MQ=255)
                                gAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGa                                                                                                 <  1:2170444/140‑1 (MQ=255)
                                   ggAAACGGCGCCATTACTCCGGACGATTTAATAAAAGATATAGAACTTCAGGGAATCAAACGTCAGCCGGGCGTATGGGACTACAAGGATTCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAgcc                                                                                              >  1:1206339/1‑138 (MQ=255)
                                   ggAAACGGCGCCATTACTCCGGACGATTTAATAAAAGATATAGAACTTCAGGGAATCAAACGTCAGCCGGGCGTATGGGACTACAAGGATTCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAgcc                                                                                              >  1:2332302/1‑138 (MQ=255)
                                   ggAAACGGCGCCATTACTCCGGACGATTTAATAAAAGATATAGAACTTCAGGGAATCAAACGTCAGCCGGGCGTATGGGACTACAAGGATTCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAgcc                                                                                              >  1:317318/1‑138 (MQ=255)
                                     aaaCGGCGCCATCACTCCGGACGATTTAAGAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCtt                                                                                            <  1:816891/140‑1 (MQ=255)
                                       aCGGCGCAATCACTCCGGACGATTTAATAAAAGACGTATAAAATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGTATTCCAATTATTCTGGTCTCGCTTATATTGTAGATGAAGTAAGCGGTGAGTCTTAt                                                                                          >  2:1532363/1‑140 (MQ=255)
                                       aCGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTAt                                                                                          >  2:972845/1‑140 (MQ=255)
                                       aCGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTAt                                                                                          >  2:1230874/1‑140 (MQ=255)
                                          gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                       <  2:2132241/140‑1 (MQ=255)
                                          gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                       <  1:1042668/140‑1 (MQ=255)
                                          gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                       <  1:809533/140‑1 (MQ=255)
                                          gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                       <  1:744318/140‑1 (MQ=255)
                                          gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                       <  1:1212110/140‑1 (MQ=255)
                                          gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                       <  1:1310854/140‑1 (MQ=255)
                                          gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                       <  1:1414071/140‑1 (MQ=255)
                                          gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                       <  1:2125370/140‑1 (MQ=255)
                                          gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                       <  1:1889372/140‑1 (MQ=255)
                                            gcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACa                                                                                     <  1:1022161/140‑1 (MQ=255)
                                                        gACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGaa                                                                         >  2:777924/1‑140 (MQ=255)
                                                          cGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATc                                                                       >  2:2417327/1‑140 (MQ=255)
                                                              ttAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                       <  1:754915/120‑1 (MQ=255)
                                                              ttAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                       >  2:80551/1‑120 (MQ=255)
                                                              ttAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                       >  2:754915/1‑120 (MQ=255)
                                                              ttAATAAAAGACGTAGAACATCAAGGAATCAAACGTCACCCTGGTGTATGGGACTATAAGTATTCCAATTATTCTGTTCTCGCTTATGTTGTAGCTGAAGTAAGCCGTGAGTCTTATGAACAATACATAACGAATCatat                                                                   >  2:1343348/1‑140 (MQ=255)
                                                                       gACGTAGAACATCAAGGTCTCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTCTTCTGGTCTCGCTTATCTGGTAGGGGATGTACGCGGTGAGTCTTATGAACAATACTGACCGAATCATATTTTTaaccg                                                          >  1:1777940/1‑137 (MQ=255)
                                                                         cGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTTTGGGTCTATAAGGATTCCAATTATTCTGTTCTCGATTATATTGTAGCTGAAGTACGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACcgg                                                        <  1:1539299/140‑1 (MQ=255)
                                                                              aaCATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGa                                                   <  2:1465078/140‑1 (MQ=255)
                                                                                   cAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTTAACCGGCGGGAATGAc                                              >  1:707071/1‑140 (MQ=255)
                                                                                   cAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGACTGAc                                              >  1:774339/1‑140 (MQ=255)
                                                                                   cAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGAc                                              >  2:1821464/1‑140 (MQ=255)
                                                                                   cAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGAc                                              >  1:1385582/1‑140 (MQ=255)
                                                                                        aaTCAAACGTGAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTATGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATg                                         <  2:827171/140‑1 (MQ=255)
                                                                                          tCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACAGGCGGGAATGACGCATGCa                                       >  1:2044321/1‑140 (MQ=255)
                                                                                           cAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                       >  2:1589127/1‑91 (MQ=255)
                                                                                           cAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                       <  1:1589127/91‑1 (MQ=255)
                                                                                           cAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATGGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAg                                      >  2:900989/1‑140 (MQ=255)
                                                                                                  cAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTta                               >  2:194155/1‑140 (MQ=255)
                                                                                                      cGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATaaa                           <  2:419525/140‑1 (MQ=255)
                                                                                                      cGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATaaa                           <  2:2304203/140‑1 (MQ=255)
                                                                                                          tgtATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACat                       >  2:614201/1‑140 (MQ=255)
                                                                                                           gtATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACata                      <  1:554539/140‑1 (MQ=255)
                                                                                                           gtATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACata                      <  1:1108837/140‑1 (MQ=255)
                                                                                                                  aCTATAAGGATTCCAATTATTCTGTTCTGGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATaaa               <  2:1397543/140‑1 (MQ=255)
                                                                                                                  aCTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATaaa               <  1:1470155/140‑1 (MQ=255)
                                                                                                                  aCTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATaaa               <  2:1502678/140‑1 (MQ=255)
                                                                                                                    tataAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATaaa                           <  1:2404982/126‑1 (MQ=255)
                                                                                                                    tataAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGa             >  1:1665978/1‑140 (MQ=255)
                                                                                                                    tataAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGa             >  1:209045/1‑140 (MQ=255)
                                                                                                                    tataAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGa             >  2:129512/1‑140 (MQ=255)
                                                                                                                      taAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAGACATATAATAAAGAAc           >  2:691914/1‑140 (MQ=255)
                                                                                                                      taAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAAc           >  1:1149612/1‑140 (MQ=255)
                                                                                                                      taAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAAc           >  1:2348897/1‑140 (MQ=255)
                                                                                                                      taAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAAc           >  2:165383/1‑140 (MQ=255)
                                                                                                                      taAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTAGGAAACATATAATAAAGAAc           >  1:1193577/1‑140 (MQ=255)
                                                                                                                           aTTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTAt      >  2:2346585/1‑140 (MQ=255)
                                                                                                                           aTTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTAt      >  1:2146414/1‑140 (MQ=255)
                                                                                                                            ttCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATc     >  2:2279583/1‑140 (MQ=255)
                                                                                                                            ttCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAGCCCTATGAACAGTACATAAAGAATCATATTTTTAAACCTGCGGGAATGACGCATGCAGGCTTTTACAAAACATATGAAAAAGAACCTTATc     <  2:1051084/140‑1 (MQ=255)
                                                                                                                            ttCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAGCCCTATGAACAGTACATAAAGAATCATATTTTTAAACCTGCGGGAATGACGCATGCAGGCTTTTACAAAACATATGAAAAAGAACCTTATc     <  2:317318/140‑1 (MQ=255)
                                                                                                                            ttCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAGCCCTATGAACAGTACATAAAGAATCATATTTTTAAACCTGCGGGAATGACGCATGCAGGCTTTTACAAAACATATGAAAAAGAACCTTATc     <  2:1716070/140‑1 (MQ=255)
                                                                                                                            ttCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAGCCCTATGAACAGTACATAAAGAATCATATTTTTAAACCTGCGGGAATGACGCATGCAGGCTTTTACAAAACATATGAAAAAGAACCTTATc     <  2:2332302/140‑1 (MQ=255)
                                                                                                                            ttCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAGCCCTATGAACAGTACATAAAGAATCATATTTTTAAACCTGCGGGAATGACGCATGCAGGCTTTTACAAAACATATGAAAAAGAACCTTATc     <  2:1206339/140‑1 (MQ=255)
                                                                                                                             tCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATcc    >  2:516783/1‑140 (MQ=255)
                                                                                                                             tCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATcc    >  1:462394/1‑140 (MQ=255)
                                                                                                                              ccAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCct   >  1:709962/1‑140 (MQ=255)
                                                                                                                              ccAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCct   >  2:200367/1‑140 (MQ=255)
                                                                                                                               cAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCctg  <  2:2401517/140‑1 (MQ=255)
                                                                                                                               cAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCctg  <  2:2312206/140‑1 (MQ=255)
                                                                                                                                 attattCTGTTCTCGCTTATAGTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATaaa               <  1:2005740/125‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                        
TTACACATACATCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTG  >  NZ_CP034943/1734267‑1734533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: