Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 344,593 G→A 5.1% K147K (AAG→AAA putR → proline utilization transcriptional regulator PutR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943344,5930GA 5.1% 428.8 / 8.8 138K147K (AAG→AAAputRproline utilization transcriptional regulator PutR
Reads supporting (aligned to +/- strand):  ref base G (69/62);  new base A (4/3);  total (73/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

Only 100 of 140 total aligned reads displayed.

TCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGTGCTGCCTTCCATGTTTTCGGTTGTCA  >  NZ_CP034943/344454‑344729
                                                                                                                                           |                                                                                                                                        
tCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAg                                                                                                                                          >  1:2361675/1‑140 (MQ=255)
     aGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGaa                                                                                                                                     >  1:517890/1‑140 (MQ=255)
      gTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAg                                                                                                                                    <  1:91775/140‑1 (MQ=255)
                   aGGGGAAGTCATGGGCTACATCTGGATTCAGGAGCTGGACCAAAATCTGACGGATGAAGAGCTCGACTTTTTGTACGAGACCTCCTTCCATGTCGGGAAAATCATTTATAAAACGAATAAATTGAAACAGGAAAAGgaag                                                                                                                       >  1:1278619/1‑140 (MQ=255)
                   aGGGGAAGTCATGGGCTACATCTGGATTCAGGAGCTGGACCAAAATCTGACGGATGAAGAGCTCGACTTTTTGTACGAGACCTCCTTCCATGTCGGGAAAATCATTTATAAAACGAATAAATTGAAACAGGAAAAGgaag                                                                                                                       >  1:1278980/1‑140 (MQ=255)
                   aGGGGAAGTCATGGGCTACATCTGGATTCAGGAGCTGGACCAAAATCTGACGGATGAAGAGCTCGACTTTTTGTACGAGACCTCCTTCCATGTCGGGAAAATCATTTATAAAACGAATAAATTGAAACAGGAAAAGgaag                                                                                                                       >  1:512300/1‑140 (MQ=255)
                    ggggAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATTTGGGTAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGgaaga                                                                                                                      <  1:332783/140‑1 (MQ=255)
                       gAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaaa                                                                                                                   >  1:1212449/1‑140 (MQ=255)
                        aaGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaaaa                                                                                                                  >  1:1352800/1‑140 (MQ=255)
                        aaGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaaaa                                                                                                                  >  1:2160852/1‑140 (MQ=255)
                        aaGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaaaa                                                                                                                  >  1:2087031/1‑140 (MQ=255)
                        aaGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCAGTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaaaa                                                                                                                  >  1:1553304/1‑140 (MQ=255)
                         aGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAg                                                                                                                 >  2:508645/1‑140 (MQ=255)
                         aGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAg                                                                                                                 >  2:1213220/1‑140 (MQ=255)
                            cATGGGCTACATCTTGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGTACTGGTCTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCTTTTATAAAACGACCAAGCTGAAGCAGGAAAAGGTAGAAAAAGCgg                                                                                                              <  2:1653395/140‑1 (MQ=255)
                             aTGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTGTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGCAAAAGGAAGAAAAATCGGa                                                                                                             >  2:207914/1‑140 (MQ=255)
                               gggCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGCAAAGGAAGAAAAAGCGGAAg                                                                                                           >  2:301548/1‑140 (MQ=255)
                               gggCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAg                                                                                                           >  2:129322/1‑140 (MQ=255)
                                 gatACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAt                                                                                                         <  2:2237701/138‑1 (MQ=255)
                                 gCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAt                                                                                                         <  2:1992608/140‑1 (MQ=255)
                                 gCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAt                                                                                                         <  2:1422390/140‑1 (MQ=255)
                                 gCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAt                                                                                                         <  1:1648870/140‑1 (MQ=255)
                                    aCATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGAtctc                                                                                                      >  1:1353455/1‑140 (MQ=255)
                                     cATCTGGGTTCAGGAGCTGGACCCAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGCAAAGGAAGAAAAAGCGGAAGATCTCa                                                                                                     >  2:1635291/1‑140 (MQ=255)
                                     cATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGACAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGACGATCTCa                                                                                                     >  2:1517513/1‑140 (MQ=255)
                                     cATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCgg                                                                                                              <  2:141672/131‑1 (MQ=255)
                                     cATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCgg                                                                                                              >  1:141672/1‑131 (MQ=255)
                                     cATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCa                                                                                                     >  2:166829/1‑140 (MQ=255)
                                     cATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCa                                                                                                     >  1:147326/1‑140 (MQ=255)
                                     cATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCa                                                                                                     >  2:804740/1‑140 (MQ=255)
                                          gggTTCAGGAGCTGGAACAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTaaa                                                                                                <  1:226394/140‑1 (MQ=255)
                                           ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGACAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAAc                                                                                               >  2:2323991/1‑140 (MQ=255)
                                           ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGaaa                                                                                                                    >  2:1298617/1‑119 (MQ=255)
                                           ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAAc                                                                                               >  2:199675/1‑140 (MQ=255)
                                           ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAAc                                                                                               >  2:1482957/1‑140 (MQ=255)
                                           ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAAc                                                                                               >  2:53412/1‑140 (MQ=255)
                                           ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAACGAAGAAAAAGCGGAAGATCACATTAAAc                                                                                               >  2:416637/1‑140 (MQ=255)
                                           ggTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGGCGGGAAAATCATTGATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAAc                                                                                               >  2:1162843/1‑140 (MQ=255)
                                              tCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACggg                                                                                            <  2:2182117/140‑1 (MQ=255)
                                              tCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACggg                                                                                            <  1:257015/140‑1 (MQ=255)
                                               cAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGc                                                                                           <  2:2361675/140‑1 (MQ=255)
                                               cAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGc                                                                                           <  2:1086800/140‑1 (MQ=255)
                                               cAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGc                                                                                           >  1:1298834/1‑140 (MQ=255)
                                                aGGAGCTGGACCAAAATCTGACGGATGAAGAGCTCGACTTTTTGTACGAGACCTCCTTCCATGTCGGGAAAATCATTTATAAAACGAATAAATTGAAACAGGAAAAGGAAGAAAAAGCAGAGGATCTCATCAAACGGTCg                                                                                          >  2:157467/1‑140 (MQ=255)
                                                aGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGc                                                                                           >  2:1898390/1‑139 (MQ=255)
                                                aGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCg                                                                                          >  2:455489/1‑140 (MQ=255)
                                                aGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCg                                                                                          >  1:1185537/1‑140 (MQ=255)
                                                aGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCACGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCg                                                                                          >  2:2378229/1‑140 (MQ=255)
                                                 ggAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGa                                                                                         <  2:1943849/140‑1 (MQ=255)
                                                 ggAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGa                                                                                         <  2:1943809/140‑1 (MQ=255)
                                                    gCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCt                                                                                      <  2:1185537/140‑1 (MQ=255)
                                                     cTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTa                                                                                     >  2:1698153/1‑140 (MQ=255)
                                                       ggACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATc                                                                                   >  1:781061/1‑140 (MQ=255)
                                                       ggACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGAAGGAAAAGGAAGAAAAAGCGGTAGATCTCATTAAAAGGGAGAtctctc                                                                                   >  2:2035925/1‑140 (MQ=255)
                                                             aaaTCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTTTCAGCAAt                                                                             >  2:1870211/1‑140 (MQ=255)
                                                             aaaTCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAAt                                                                             >  2:13114/1‑140 (MQ=255)
                                                             aaaTCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAAt                                                                             >  2:1730250/1‑140 (MQ=255)
                                                                tCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTa                                                                          <  1:1244396/140‑1 (MQ=255)
                                                                tCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTa                                                                          <  2:1575945/140‑1 (MQ=255)
                                                                 cTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTAc                                                                         <  2:2298500/140‑1 (MQ=255)
                                                                   gACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCa                                                                       >  2:832963/1‑140 (MQ=255)
                                                                    aCGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCAc                                                                      <  1:1443870/140‑1 (MQ=255)
                                                                    aCGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCAc                                                                      <  1:1756936/140‑1 (MQ=255)
                                                                     cGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACt                                                                     <  1:455489/140‑1 (MQ=255)
                                                                      ggAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTg                                                                    <  2:1454403/140‑1 (MQ=255)
                                                                         agaagaaCTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTgaga                                                                 <  1:1383945/140‑1 (MQ=255)
                                                                         agaagaaCTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTgaga                                                                 <  1:1572318/140‑1 (MQ=255)
                                                                              aaCTCGACTTTTTGTATGAGACCTATTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGaa                                                            <  1:2402241/140‑1 (MQ=255)
                                                                                     cTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGAc                                                     >  1:217623/1‑140 (MQ=255)
                                                                                     cTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGAc                                                     >  1:723/1‑140 (MQ=255)
                                                                                        tttGTACGAGACCTCCTTCCATGTCGGGAAAATCATTTATAAAACGAATAAATTGAAACAGGAAAAGGAAGAAAAAGCAGAGGATCTCATCAAACGGGCGATCTATCAGCAATTTACCTCTGAGAAGGAACTCAGACggg                                                  <  2:512300/140‑1 (MQ=255)
                                                                                        tttGTACGAGACCTCCTTCCATGTCGGGAAAATCATTTATAAAACGAATAAATTGAAACAGGAAAAGGAAGAAAAAGCAGAGGATCTCATCAAACGGGCGATCTATCAGCAATTTACCTCTGAGAAGGAACTCAGACggg                                                  <  2:1278980/140‑1 (MQ=255)
                                                                                          tGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGaa                                                >  1:875506/1‑140 (MQ=255)
                                                                                               gagaCCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGa                                           >  2:804530/1‑140 (MQ=255)
                                                                                                agaCCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCTGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGaa                                          <  1:3050/140‑1 (MQ=255)
                                                                                                agaCCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGaa                                          <  1:1780566/140‑1 (MQ=255)
                                                                                                  aCCTCTTTTCATGTCGTGAAAATCATTTATAAAAAGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAg                                        <  2:1441695/140‑1 (MQ=255)
                                                                                                  aCCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAg                                        >  2:963355/1‑140 (MQ=255)
                                                                                                  aCCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAg                                        >  2:963105/1‑140 (MQ=255)
                                                                                                    ctctTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGaa                                      >  2:1781053/1‑140 (MQ=255)
                                                                                                        tttCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCaa                                  <  1:1981343/140‑1 (MQ=255)
                                                                                                        tttCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCaa                                  <  1:508645/140‑1 (MQ=255)
                                                                                                         ttCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAAATCAGACGGGAAGCCGAAATAATCAac                                 >  2:2414432/1‑140 (MQ=255)
                                                                                                         ttCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCAGAATATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAac                                 >  2:1154434/1‑140 (MQ=255)
                                                                                                         ttCATGTCGGGAAAATCAGTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCAATGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAac                                 >  2:2414198/1‑140 (MQ=255)
                                                                                                          tCATTTCGTGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAaca                                <  2:1331248/140‑1 (MQ=255)
                                                                                                          tCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAaca                                <  1:832963/140‑1 (MQ=255)
                                                                                                          tCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAaca                                <  1:963355/140‑1 (MQ=255)
                                                                                                              gTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGACGCCGAAAGAATCAACACCGt                            >  1:1968478/1‑140 (MQ=255)
                                                                                                                cGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAACAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGtgc                          <  1:1299544/140‑1 (MQ=255)
                                                                                                                cGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGtgc                          <  1:2274684/140‑1 (MQ=255)
                                                                                                                cGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGtgc                          <  1:1299622/140‑1 (MQ=255)
                                                                                                                 gggAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGtgct                         >  2:752874/1‑140 (MQ=255)
                                                                                                                        tCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTACACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGACTCAACACCGTGCTGCCTTcc                  <  1:260455/140‑1 (MQ=255)
                                                                                                                                aaaaCGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGTGCTGCCTTCCATGTTTTc          <  2:1457787/140‑1 (MQ=255)
                                                                                                                                  aaCGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGTGCTGCCTTCCATGTTTTCgg        >  1:2263217/1‑140 (MQ=255)
                                                                                                                                     gAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGTGCTGCCTTCCATGTTTTCGGTTg     <  1:1482957/140‑1 (MQ=255)
                                                                                                                                     gAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGTGCTGCCTTCCATGTTTTCGGTTg     <  2:147326/140‑1 (MQ=255)
                                                                                                                                        cAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGTGCTGCCTTCCATGTTTTCGGTTGTCa  <  2:716562/140‑1 (MQ=255)
                                                                                                                                        cAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGTGCTGCCTTCCATGTTTTCGGTTGTCa  <  2:1019004/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                        
TCGTGAGTGCGAAGCACAAAGGGGAAGTCATGGGCTACATCTGGGTTCAGGAGCTGGACCAAAATCTGACGGAAGAAGAACTCGACTTTTTGTATGAGACCTCTTTTCATGTCGGGAAAATCATTTATAAAACGAACAAGCTGAAGCAGGAAAAGGAAGAAAAAGCGGAAGATCTCATTAAACGGGCGATCTATCAGCAATTTACCACTGAGAAGGAACTCAGACGGGAAGCCGAAAGAATCAACACCGTGCTGCCTTCCATGTTTTCGGTTGTCA  >  NZ_CP034943/344454‑344729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: