Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,734,409 C→T 8.9% L225L (CTC→CTT EO946_RS09000 → serine hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,734,4090CT 8.9% 479.8 / 26.5 159L225L (CTC→CTTEO946_RS09000serine hydrolase
Reads supporting (aligned to +/- strand):  ref base C (72/72);  new base T (7/7);  total (79/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01

Only 100 of 161 total aligned reads displayed.

CACATACATCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCGGGTATA  >  NZ_CP034943/1734270‑1734545
                                                                                                                                           |                                                                                                                                        
cacaTACATCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCCAGGAATCAAAAGTCAGCCGGGTGTATGGTACTCTAAGGATTCCAATTATTCTGTtctc                                                                                                                                          >  2:1903640/1‑140 (MQ=255)
     acatCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAATGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTta                                                                                                                                     >  1:2133366/1‑140 (MQ=255)
     acatCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTta                                                                                                                                     <  1:235745/140‑1 (MQ=255)
     acatCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTta                                                                                                                                     <  1:2150765/140‑1 (MQ=255)
      catCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCttct                                                                                                                                    >  2:2064685/1‑138 (MQ=255)
        tCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTatat                                                                                                                                  <  2:728356/140‑1 (MQ=255)
        tCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTatat                                                                                                                                  <  2:1560253/140‑1 (MQ=255)
        tCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAACAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTatat                                                                                                                                  <  2:1836172/140‑1 (MQ=255)
         cGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATAtt                                                                                                                                 <  2:2215602/140‑1 (MQ=255)
         cGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATAtt                                                                                                                                 <  2:1285118/140‑1 (MQ=255)
           ggAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGt                                                                                                                               >  1:2231377/1‑140 (MQ=255)
           ggAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGt                                                                                                                               >  1:408925/1‑140 (MQ=255)
           ggAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGt                                                                                                                               >  1:714838/1‑140 (MQ=255)
                 aaCGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGa                                                                                                                         >  2:744318/1‑140 (MQ=255)
                      tCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTaa                                                                                                                    >  1:917600/1‑140 (MQ=255)
                      tCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTaa                                                                                                                    >  1:458943/1‑140 (MQ=255)
                           ttGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGt                                                                                                               <  2:418251/140‑1 (MQ=255)
                             gAAGGAAACGGCGCACTCACTCCGGACGATTAAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGa                                                                                                             <  1:2298244/140‑1 (MQ=255)
                             gAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGa                                                                                                             <  1:1194829/140‑1 (MQ=255)
                             gAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGa                                                                                                             <  1:2170444/140‑1 (MQ=255)
                             gAAGCAAACGGCGCAATCACACCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAACCGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGa                                                                                                             <  1:2014570/140‑1 (MQ=255)
                                ggAAACGGCGCCATTACTCCGGACGATTTAATAAAAGATATAGAACTTCAGGGAATCAAACGTCAGCCGGGCGTATGGGACTACAAGGATTCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAgcc                                                                                                          >  1:1206339/1‑138 (MQ=255)
                                ggAAACGGCGCCATTACTCCGGACGATTTAATAAAAGATATAGAACTTCAGGGAATCAAACGTCAGCCGGGCGTATGGGACTACAAGGATTCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAgcc                                                                                                          >  1:1716070/1‑138 (MQ=255)
                                ggAAACGGCGCCATTACTCCGGACGATTTAATAAAAGATATAGAACTTCAGGGAATCAAACGTCAGCCGGGCGTATGGGACTACAAGGATTCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAgcc                                                                                                          >  1:317318/1‑138 (MQ=255)
                                ggAAACGGCGCCATTACTCCGGACGATTTAATAAAAGATATAGAACTTCAGGGAATCAAACGTCAGCCGGGCGTATGGGACTACAAGGATTCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAgcc                                                                                                          >  1:2332302/1‑138 (MQ=255)
                                  aaaCGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCtt                                                                                                        <  1:55034/140‑1 (MQ=255)
                                    aCGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTAt                                                                                                      >  2:972845/1‑140 (MQ=255)
                                       gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                   <  1:809533/140‑1 (MQ=255)
                                       gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                   <  2:2132241/140‑1 (MQ=255)
                                       gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                   <  1:1042668/140‑1 (MQ=255)
                                       gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                   <  1:2125370/140‑1 (MQ=255)
                                       gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                   <  1:1414071/140‑1 (MQ=255)
                                       gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                   <  1:1310854/140‑1 (MQ=255)
                                       gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                   <  1:1212110/140‑1 (MQ=255)
                                       gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                   <  1:155911/140‑1 (MQ=255)
                                       gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                   <  1:1889372/140‑1 (MQ=255)
                                         gcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACa                                                                                                 <  1:1022161/140‑1 (MQ=255)
                                                     gACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGaa                                                                                     >  2:777924/1‑140 (MQ=255)
                                                       cGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATc                                                                                   >  2:2417327/1‑140 (MQ=255)
                                                           ttAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                   >  2:80551/1‑120 (MQ=255)
                                                           ttAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                   <  1:80551/120‑1 (MQ=255)
                                                           ttAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                   <  1:754915/120‑1 (MQ=255)
                                                           ttAATAAAAGACGTAGAACATCAAGGAATCAAACGTCACCCTGGTGTATGGGACTATAAGTATTCCAATTATTCTGTTCTCGCTTATGTTGTAGCTGAAGTAAGCCGTGAGTCTTATGAACAATACATAACGAATCatat                                                                               >  2:1343348/1‑140 (MQ=255)
                                                                                cAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTTAACCGGCGGGAATGAc                                                          >  1:707071/1‑140 (MQ=255)
                                                                                cAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGAc                                                          >  1:1477942/1‑140 (MQ=255)
                                                                                cAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGAc                                                          >  1:1385582/1‑140 (MQ=255)
                                                                                cAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGAc                                                          >  2:616863/1‑140 (MQ=255)
                                                                                  aGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCGGAAGTAAGCGGTGAGTCTTATGAACAATACATAACTAATCAGATTTTTAAACCGGAGGGAATGACga                                                        >  1:2417891/1‑139 (MQ=255)
                                                                                     aaTCAAACGTGAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTATGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATg                                                     <  2:827171/140‑1 (MQ=255)
                                                                                       tCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACAGGCGGGAATGACGCATGCa                                                   >  1:2044321/1‑140 (MQ=255)
                                                                                        cAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                   <  1:1589127/91‑1 (MQ=255)
                                                                                        cAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                   >  2:1589127/1‑91 (MQ=255)
                                                                                        cAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATGGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAg                                                  >  2:900989/1‑140 (MQ=255)
                                                                                               cAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTta                                           >  2:194155/1‑140 (MQ=255)
                                                                                                   cGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTGTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATaaa                                       <  1:1903640/140‑1 (MQ=255)
                                                                                                   cGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATaaa                                       <  2:2259346/140‑1 (MQ=255)
                                                                                                   cGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATaaa                                       <  2:419525/140‑1 (MQ=255)
                                                                                                       tgtATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACat                                   >  2:614201/1‑140 (MQ=255)
                                                                                                        gtATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACata                                  <  1:554539/140‑1 (MQ=255)
                                                                                                        gtATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACata                                  <  1:1108837/140‑1 (MQ=255)
                                                                                                           tGGGACTATAAGGATTCCAATTATTCTGTTCTCGATTATATTGTAGCTGAAGTAAGCGTTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCTGGAATGACTCATGCAGGCTTTTATAAAACATataa                               >  1:839822/1‑140 (MQ=255)
                                                                                                               aCTATAAGGATTCCTATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATaaa                           <  2:319426/140‑1 (MQ=255)
                                                                                                               aCTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATaaa                           <  2:1502678/140‑1 (MQ=255)
                                                                                                               aCTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATaaa                           <  1:1470155/140‑1 (MQ=255)
                                                                                                                cTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATCATAAAg                          >  2:1231054/1‑140 (MQ=255)
                                                                                                                 tataAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATaaa                                       <  1:2404982/126‑1 (MQ=255)
                                                                                                                 tataAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGa                         >  2:25662/1‑140 (MQ=255)
                                                                                                                 tataAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGa                         >  2:1488742/1‑140 (MQ=255)
                                                                                                                 tataAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGa                         >  1:1665978/1‑140 (MQ=255)
                                                                                                                   taAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAAc                       >  1:2348897/1‑140 (MQ=255)
                                                                                                                   taAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAAc                       >  2:437637/1‑140 (MQ=255)
                                                                                                                   taAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAAc                       >  2:1416232/1‑140 (MQ=255)
                                                                                                                   taAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAAc                       >  2:12805/1‑140 (MQ=255)
                                                                                                                   taAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAAc                       >  1:766353/1‑140 (MQ=255)
                                                                                                                   taAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTAGGAAACATATAATAAAGAAc                       >  1:1193577/1‑140 (MQ=255)
                                                                                                                   taAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCGGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAAc                       >  1:1148982/1‑140 (MQ=255)
                                                                                                                        agtCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTAt                  >  2:1714512/3‑140 (MQ=255)
                                                                                                                         ttCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATc                 >  2:2279583/1‑140 (MQ=255)
                                                                                                                         ttCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAGCCCTATGAACAGTACATAAAGAATCATATTTTTAAACCTGCGGGAATGACGCATGCAGGCTTTTACAAAACATATGAAAAAGAACCTTATc                 <  2:2332302/140‑1 (MQ=255)
                                                                                                                         ttCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAGCCCTATGAACAGTACATAAAGAATCATATTTTTAAACCTGCGGGAATGACGCATGCAGGCTTTTACAAAACATATGAAAAAGAACCTTATc                 <  2:2332797/140‑1 (MQ=255)
                                                                                                                         ttCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAGCCCTATGAACAGTACATAAAGAATCATATTTTTAAACCTGCGGGAATGACGCATGCAGGCTTTTACAAAACATATGAAAAAGAACCTTATc                 <  2:1051084/140‑1 (MQ=255)
                                                                                                                         ttCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAGCCCTATGAACAGTACATAAAGAATCATATTTTTAAACCTGCGGGAATGACGCATGCAGGCTTTTACAAAACATATGAAAAAGAACCTTATc                 <  2:1206339/140‑1 (MQ=255)
                                                                                                                         ttCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAGCCCTATGAACAGTACATAAAGAATCATATTTTTAAACCTGCGGGAATGACGCATGCAGGCTTTTACAAAACATATGAAAAAGAACCTTATc                 <  2:317318/140‑1 (MQ=255)
                                                                                                                         ttCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAGCCCTATGAACAGTACATAAAGAATCATATTTTTAAACCTGCGGGAATGACGCATGCAGGCTTTTACAAAACATATGAAAAAGAACCTTATc                 <  2:1330049/140‑1 (MQ=255)
                                                                                                                         ttCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAGCCCTATGAACAGTACATAAAGAATCATATTTTTAAACCTGCGGGAATGACGCATGCAGGCTTTTACAAAACATATGAAAAAGAACCTTATc                 <  2:1716070/140‑1 (MQ=255)
                                                                                                                          tCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATcc                >  1:462394/1‑140 (MQ=255)
                                                                                                                          tCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATcc                >  1:1835187/1‑140 (MQ=255)
                                                                                                                          tCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATcc                >  1:2044667/1‑140 (MQ=255)
                                                                                                                           ccAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCct               >  2:200367/1‑140 (MQ=255)
                                                                                                                            cAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCctg              <  2:2312206/140‑1 (MQ=255)
                                                                                                                            cAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCctg              <  2:2401517/140‑1 (MQ=255)
                                                                                                                              attattCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATaaa                           <  1:2105220/125‑1 (MQ=255)
                                                                                                                              attattCTGTTCTCGCTTATACTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATaaa                           >  2:2005740/1‑125 (MQ=255)
                                                                                                                                tattCTGTTCTCGCTTATATTGTAGCTGAAGTAGGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGt          <  2:783151/140‑1 (MQ=255)
                                                                                                                                tattCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGt          <  2:709962/140‑1 (MQ=255)
                                                                                                                                tattCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGt          <  2:1169847/140‑1 (MQ=255)
                                                                                                                                tattCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGt          <  1:6298/140‑1 (MQ=255)
                                                                                                                                  ttCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCg        >  1:1635512/1‑140 (MQ=255)
                                                                                                                                       ttCTCGCTTATAATGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAAGCGCCGGGAATGACGCATGAAGGCTTTTATAAAACATAGAATACAGAACCTTATCCTGCTGTCGGGtat   >  2:1644124/1‑140 (MQ=255)
                                                                                                                                        tctcGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCGGGtata  <  1:1154133/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                        
CACATACATCGGGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCGGGTATA  >  NZ_CP034943/1734270‑1734545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: