Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,734,419 G→A 9.5% V229I (GTA→ATA) ‡ EO946_RS09000 → serine hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,734,4190GA 9.5% 436.7 / 27.0 147V229I (GTA→ATA) ‡EO946_RS09000serine hydrolase
Reads supporting (aligned to +/- strand):  ref base G (64/69);  new base A (7/7);  total (71/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.93e-01

Only 100 of 147 total aligned reads displayed.

GGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCGGGTATAAGCTGAAGGGCA  >  NZ_CP034943/1734281‑1734557
                                                                                                                                          |                                                                                                                                          
ggAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGt                                                                                                                                           >  1:2231377/1‑140 (MQ=255)
ggAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGt                                                                                                                                           >  1:408925/1‑140 (MQ=255)
ggAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATGGt                                                                                                                                           >  2:388052/1‑140 (MQ=255)
    tAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGTACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCt                                                                                                                                       <  1:91577/140‑1 (MQ=255)
           tCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTAATATTGTAGCTGAAGTaa                                                                                                                                >  1:460329/1‑140 (MQ=255)
                ttGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGt                                                                                                                           <  1:202359/140‑1 (MQ=255)
                ttGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGt                                                                                                                           <  2:2231377/140‑1 (MQ=255)
                ttGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGt                                                                                                                           <  2:418251/140‑1 (MQ=255)
                  gcaGGCAACGGCGCAATCACTCCGGACGAGTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGa                                                                                                                         <  1:1879013/138‑1 (MQ=255)
                  gAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGa                                                                                                                         <  2:2326145/140‑1 (MQ=255)
                  gAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGa                                                                                                                         <  1:2125555/140‑1 (MQ=255)
                  gAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGa                                                                                                                         <  1:2170444/140‑1 (MQ=255)
                     ggAAACGGCGCCATTACTCCGGACGATTTAATAAAAGATATAGAACTTCAGGGAATCAAACGTCAGCCGGGCGTATGGGACTACAAGGATTCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAgcc                                                                                                                      >  1:1206339/1‑138 (MQ=255)
                     ggAAACGGCGCCATTACTCCGGACGATTTAATAAAAGATATAGAACTTCAGGGAATCAAACGTCAGCCGGGCGTATGGGACTACAAGGATTCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAgcc                                                                                                                      >  1:1716070/1‑138 (MQ=255)
                     ggAAACGGCGCCATTACTCCGGACGATTTAATAAAAGATATAGAACTTCAGGGAATCAAACGTCAGCCGGGCGTATGGGACTACAAGGATTCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAgcc                                                                                                                      >  1:1051084/1‑138 (MQ=255)
                     ggAAACGGCGCCATTACTCCGGACGATTTAATAAAAGATATAGAACTTCAGGGAATCAAACGTCAGCCGGGCGTATGGGACTACAAGGATTCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAgcc                                                                                                                      >  1:317318/1‑138 (MQ=255)
                     ggAAACGGCGCCATTACTCCGGACGATTTAATAAAAGATATAGAACTTCAGGGAATCAAACGTCAGCCGGGCGTATGGGACTACAAGGATTCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAgcc                                                                                                                      >  1:2332797/1‑138 (MQ=255)
                     ggAAACGGCGCCATTACTCCGGACGATTTAATAAAAGATATAGAACTTCAGGGAATCAAACGTCAGCCGGGCGTATGGGACTACAAGGATTCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAgcc                                                                                                                      >  1:2332302/1‑138 (MQ=255)
                       aaaCGGCGCAATCACTCCGGACGATTTAATAAAAGAAGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCtt                                                                                                                    <  1:1161354/140‑1 (MQ=255)
                         aCGGCGCAATCACTCCGGACGATTTAATAAAAGACGTATAAAATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGTATTCCAATTATTCTGGTCTCGCTTATATTGTAGATGAAGTAAGCGGTGAGTCTTAt                                                                                                                  >  2:1532363/1‑140 (MQ=255)
                         aCGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTAt                                                                                                                  >  2:972845/1‑140 (MQ=255)
                         aCGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTAt                                                                                                                  >  2:1230874/1‑140 (MQ=255)
                            gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                               <  1:744318/140‑1 (MQ=255)
                            gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                               <  1:809533/140‑1 (MQ=255)
                            gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                               <  2:2132241/140‑1 (MQ=255)
                            gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                               <  1:2125370/140‑1 (MQ=255)
                            gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                               <  1:1042668/140‑1 (MQ=255)
                            gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                               <  1:1212110/140‑1 (MQ=255)
                            gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                               <  1:1310854/140‑1 (MQ=255)
                            gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                               <  1:1458740/140‑1 (MQ=255)
                            gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                               <  1:155911/140‑1 (MQ=255)
                              gcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACa                                                                                                             <  1:1022161/140‑1 (MQ=255)
                                          gACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGaa                                                                                                 >  2:777924/1‑140 (MQ=255)
                                            cGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATc                                                                                               >  2:2417327/1‑140 (MQ=255)
                                                ttAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                               >  2:80551/1‑120 (MQ=255)
                                                ttAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                               >  2:754915/1‑120 (MQ=255)
                                                ttAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                               <  1:80551/120‑1 (MQ=255)
                                                ttAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                               <  1:754915/120‑1 (MQ=255)
                                                ttAATAAAAGACGTAGAACATCAAGGAATCAAACGTCACCCTGGTGTATGGGACTATAAGTATTCCAATTATTCTGTTCTCGCTTATGTTGTAGCTGAAGTAAGCCGTGAGTCTTATGAACAATACATAACGAATCatat                                                                                           >  2:1343348/1‑140 (MQ=255)
                                                           cGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTTTGGGTCTATAAGGATTCCAATTATTCTGTTCTCGATTATATTGTAGCTGAAGTACGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACcgg                                                                                <  1:1539299/140‑1 (MQ=255)
                                                           cGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACcgg                                                                                <  1:1308288/140‑1 (MQ=255)
                                                                aaCATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGa                                                                           <  2:1465078/140‑1 (MQ=255)
                                                                     cAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTTAACCGGCGGGAATGAc                                                                      >  1:707071/1‑140 (MQ=255)
                                                                     cAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGACTGAc                                                                      >  1:774339/1‑140 (MQ=255)
                                                                     cAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGAc                                                                      >  2:616863/1‑140 (MQ=255)
                                                                     cAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGAc                                                                      >  1:1385582/1‑140 (MQ=255)
                                                                     cAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGAc                                                                      >  2:1821464/1‑140 (MQ=255)
                                                                       aGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCGGAAGTAAGCGGTGAGTCTTATGAACAATACATAACTAATCAGATTTTTAAACCGGAGGGAATGACga                                                                    >  1:2417891/1‑139 (MQ=255)
                                                                          actCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTACGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATg                                                                 <  2:71098/138‑1 (MQ=255)
                                                                          aaTCAAACGTGAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTATGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATg                                                                 <  2:827171/140‑1 (MQ=255)
                                                                            tCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACAGGCGGGAATGACGCATGCa                                                               >  1:2044321/1‑140 (MQ=255)
                                                                             cAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                               >  2:1589127/1‑91 (MQ=255)
                                                                                        cGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTGTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATaaa                                                   <  1:1903640/140‑1 (MQ=255)
                                                                                        cGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATaaa                                                   <  2:419525/140‑1 (MQ=255)
                                                                                        cGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATaaa                                                   <  2:2304203/140‑1 (MQ=255)
                                                                                        cGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATaaa                                                   <  2:791958/140‑1 (MQ=255)
                                                                                            tgtATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACat                                               >  2:614201/1‑140 (MQ=255)
                                                                                             gtATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACata                                              <  1:554539/140‑1 (MQ=255)
                                                                                             gtATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACata                                              <  1:612258/140‑1 (MQ=255)
                                                                                               aTGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACAtata                                            <  1:2189121/140‑1 (MQ=255)
                                                                                                tGGGACTATAAGGATTCCAATTATTCTGTTCTCGATTATATTGTAGCTGAAGTAAGCGTTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCTGGAATGACTCATGCAGGCTTTTATAAAACATataa                                           >  1:839822/1‑140 (MQ=255)
                                                                                                    aCTATAAGGATTCCTATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATaaa                                       <  2:319426/140‑1 (MQ=255)
                                                                                                    aCTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATaaa                                       <  2:1502678/140‑1 (MQ=255)
                                                                                                    aCTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATaaa                                       <  1:1470155/140‑1 (MQ=255)
                                                                                                      tataAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATaaa                                                   <  1:2404982/126‑1 (MQ=255)
                                                                                                      tataAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGa                                     >  2:25662/1‑140 (MQ=255)
                                                                                                      tataAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGa                                     >  1:209045/1‑140 (MQ=255)
                                                                                                      tataAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGa                                     >  1:1665978/1‑140 (MQ=255)
                                                                                                        taAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAAc                                   >  1:1149612/1‑140 (MQ=255)
                                                                                                        taAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAAc                                   >  2:12805/1‑140 (MQ=255)
                                                                                                        taAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAAc                                   >  2:924426/1‑140 (MQ=255)
                                                                                                        taAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAAc                                   >  2:165383/1‑140 (MQ=255)
                                                                                                        taAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAAc                                   >  2:437637/1‑140 (MQ=255)
                                                                                                        taAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAAc                                   >  2:1416232/1‑140 (MQ=255)
                                                                                                        taAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTAGGAAACATATAATAAAGAAc                                   >  1:1193577/1‑140 (MQ=255)
                                                                                                        taAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCGGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAAc                                   >  1:1148982/1‑140 (MQ=255)
                                                                                                             aTTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTAt                              >  2:2346585/1‑140 (MQ=255)
                                                                                                              ttCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATc                             >  2:2279583/1‑140 (MQ=255)
                                                                                                              ttCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAGCCCTATGAACAGTACATAAAGAATCATATTTTTAAACCTGCGGGAATGACGCATGCAGGCTTTTACAAAACATATGAAAAAGAACCTTATc                             <  2:1051084/140‑1 (MQ=255)
                                                                                                              ttCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAGCCCTATGAACAGTACATAAAGAATCATATTTTTAAACCTGCGGGAATGACGCATGCAGGCTTTTACAAAACATATGAAAAAGAACCTTATc                             <  2:2332302/140‑1 (MQ=255)
                                                                                                              ttCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAGCCCTATGAACAGTACATAAAGAATCATATTTTTAAACCTGCGGGAATGACGCATGCAGGCTTTTACAAAACATATGAAAAAGAACCTTATc                             <  2:1716070/140‑1 (MQ=255)
                                                                                                              ttCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAGCCCTATGAACAGTACATAAAGAATCATATTTTTAAACCTGCGGGAATGACGCATGCAGGCTTTTACAAAACATATGAAAAAGAACCTTATc                             <  2:1206339/140‑1 (MQ=255)
                                                                                                              ttCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAGCCCTATGAACAGTACATAAAGAATCATATTTTTAAACCTGCGGGAATGACGCATGCAGGCTTTTACAAAACATATGAAAAAGAACCTTATc                             <  2:1330049/140‑1 (MQ=255)
                                                                                                               tCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATcc                            >  1:462394/1‑140 (MQ=255)
                                                                                                               tCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATcc                            >  1:1835187/1‑140 (MQ=255)
                                                                                                                ccAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCct                           >  2:200367/1‑140 (MQ=255)
                                                                                                                ccAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCct                           >  1:709962/1‑140 (MQ=255)
                                                                                                                 cAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCctg                          <  2:2401517/140‑1 (MQ=255)
                                                                                                                   attattCTGTTCTCGCTTATAGTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATaaa                                       <  1:2005740/125‑1 (MQ=255)
                                                                                                                   attattCTGTTCTCGCTTATACTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATaaa                                       >  2:2005740/1‑125 (MQ=255)
                                                                                                                     tattCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGt                      <  2:709962/140‑1 (MQ=255)
                                                                                                                     tattCGGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGt                      <  2:1127276/140‑1 (MQ=255)
                                                                                                                       ttCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCg                    >  1:1635512/1‑140 (MQ=255)
                                                                                                                            ttCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCGGGtat               <  1:1640818/140‑1 (MQ=255)
                                                                                                                            ttCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCGGGtat               <  1:1576795/140‑1 (MQ=255)
                                                                                                                            ttCTCGCTTATAATGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAAGCGCCGGGAATGACGCATGAAGGCTTTTATAAAACATAGAATACAGAACCTTATCCTGCTGTCGGGtat               >  2:1644124/1‑140 (MQ=255)
                                                                                                                                     atatGGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAATCCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATACAGACGCTTATCCTGCTGTCGGGTATAAGCTGAAg      <  1:2051208/140‑1 (MQ=255)
                                                                                                                                       atTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCGGTTATAAGCTGAAggg    <  1:1343348/140‑1 (MQ=255)
                                                                                                                                       agtGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCGGGTATAAGCTGAAggg    <  2:885851/138‑1 (MQ=255)
                                                                                                                                         tGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCTGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCGGGTATAAGCTGAAGGgca  >  2:1266985/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                          
GGAATAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCGGGTATAAGCTGAAGGGCA  >  NZ_CP034943/1734281‑1734557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: