Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,734,421 A→T 8.7% V229V (GTA→GTT) ‡ EO946_RS09000 → serine hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,734,4210AT 8.7% 504.5 / 30.3 157V229V (GTA→GTT) ‡EO946_RS09000serine hydrolase
Reads supporting (aligned to +/- strand):  ref base A (72/71);  new base T (7/7);  total (79/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

Only 100 of 157 total aligned reads displayed.

TAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCGGGTATAAGCTGAAGGGCAGC  >  NZ_CP034943/1734285‑1734559
                                                                                                                                        |                                                                                                                                          
tAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGTACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCt                                                                                                                                         <  1:91577/140‑1 (MQ=255)
  aaCGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGa                                                                                                                                       >  2:744318/1‑140 (MQ=255)
       tCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTAATATTGTAGCTGAAGTaa                                                                                                                                  >  1:460329/1‑140 (MQ=255)
              gAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGa                                                                                                                           <  2:2326145/140‑1 (MQ=255)
              gAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGa                                                                                                                           <  1:1194829/140‑1 (MQ=255)
              gAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGa                                                                                                                           <  1:2170444/140‑1 (MQ=255)
              gAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGa                                                                                                                           <  1:2125555/140‑1 (MQ=255)
              gAAGCAAACGGCGCAATCACACCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAACCGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGa                                                                                                                           <  1:2014570/140‑1 (MQ=255)
                 ggAAACGGCGCCATTACTCCGGACGATTTAATAAAAGATATAGAACTTCAGGGAATCAAACGTCAGCCGGGCGTATGGGACTACAAGGATTCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAgcc                                                                                                                        >  1:317318/1‑138 (MQ=255)
                 ggAAACGGCGCCATTACTCCGGACGATTTAATAAAAGATATAGAACTTCAGGGAATCAAACGTCAGCCGGGCGTATGGGACTACAAGGATTCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAgcc                                                                                                                        >  1:2332797/1‑138 (MQ=255)
                 ggAAACGGCGCCATTACTCCGGACGATTTAATAAAAGATATAGAACTTCAGGGAATCAAACGTCAGCCGGGCGTATGGGACTACAAGGATTCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAgcc                                                                                                                        >  1:1330049/1‑138 (MQ=255)
                 ggAAACGGCGCCATTACTCCGGACGATTTAATAAAAGATATAGAACTTCAGGGAATCAAACGTCAGCCGGGCGTATGGGACTACAAGGATTCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAgcc                                                                                                                        >  1:2332302/1‑138 (MQ=255)
                 ggAAACGGCGCCATTACTCCGGACGATTTAATAAAAGATATAGAACTTCAGGGAATCAAACGTCAGCCGGGCGTATGGGACTACAAGGATTCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAgcc                                                                                                                        >  1:1716070/1‑138 (MQ=255)
                   aaaCGGCGCCATCACTCCGGACGATTTAAGAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCtt                                                                                                                      <  1:816891/140‑1 (MQ=255)
                   aaaCGGCGCAATCACTCCGGACGATTTAATAAAAGAAGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCtt                                                                                                                      <  1:1161354/140‑1 (MQ=255)
                     aCGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTAt                                                                                                                    >  2:1230874/1‑140 (MQ=255)
                     aCGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTAt                                                                                                                    >  2:972845/1‑140 (MQ=255)
                        gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                                 <  1:1042668/140‑1 (MQ=255)
                        gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                                 <  1:1212110/140‑1 (MQ=255)
                        gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                                 <  1:744318/140‑1 (MQ=255)
                        gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                                 <  1:1414071/140‑1 (MQ=255)
                        gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                                 <  1:1458740/140‑1 (MQ=255)
                        gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                                 <  1:2125370/140‑1 (MQ=255)
                        gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                                 <  1:1889372/140‑1 (MQ=255)
                        gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                                 <  2:2132241/140‑1 (MQ=255)
                        gcgcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                                 <  1:155911/140‑1 (MQ=255)
                          gcAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACa                                                                                                               <  1:1022161/140‑1 (MQ=255)
                                        cGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATc                                                                                                 >  2:2417327/1‑140 (MQ=255)
                                            ttAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                                 >  2:80551/1‑120 (MQ=255)
                                            ttAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                                 >  2:754915/1‑120 (MQ=255)
                                            ttAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                                 <  1:754915/120‑1 (MQ=255)
                                            ttAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                                 <  1:80551/120‑1 (MQ=255)
                                                       cGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACcgg                                                                                  <  1:1308288/140‑1 (MQ=255)
                                                                 cAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTTAACCGGCGGGAATGAc                                                                        >  1:707071/1‑140 (MQ=255)
                                                                 cAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGACTGAc                                                                        >  1:774339/1‑140 (MQ=255)
                                                                 cAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGAc                                                                        >  2:616863/1‑140 (MQ=255)
                                                                 cAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGAc                                                                        >  1:1477942/1‑140 (MQ=255)
                                                                 cAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGAc                                                                        >  2:1821464/1‑140 (MQ=255)
                                                                        tCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACAGGCGGGAATGACGCATGCa                                                                 >  1:2044321/1‑140 (MQ=255)
                                                                         cAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGaa                                                                                                                 >  2:1589127/1‑91 (MQ=255)
                                                                         cAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATGGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAg                                                                >  2:900989/1‑140 (MQ=255)
                                                                                    cGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTGTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATaaa                                                     <  1:1903640/140‑1 (MQ=255)
                                                                                    cGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATaaa                                                     <  2:419525/140‑1 (MQ=255)
                                                                                    cGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATaaa                                                     <  2:2304203/140‑1 (MQ=255)
                                                                                    cGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATaaa                                                     <  2:2259346/140‑1 (MQ=255)
                                                                                    cGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATaaa                                                     <  2:81640/140‑1 (MQ=255)
                                                                                    cGGGTGTATGGGAATATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTATCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATaaa                                                     <  2:419221/140‑1 (MQ=255)
                                                                                        tgtATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACat                                                 >  2:614201/1‑140 (MQ=255)
                                                                                         gtATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACata                                                <  1:554539/140‑1 (MQ=255)
                                                                                         gtATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACata                                                <  1:612258/140‑1 (MQ=255)
                                                                                         gtATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACata                                                <  1:1108837/140‑1 (MQ=255)
                                                                                           aTGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACAtata                                              <  1:2189121/140‑1 (MQ=255)
                                                                                            tGGGACTATAAGGATTCCAATTATTCTGTTCTCGATTATATTGTAGCTGAAGTAAGCGTTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCTGGAATGACTCATGCAGGCTTTTATAAAACATataa                                             >  1:839822/1‑140 (MQ=255)
                                                                                                aCTATAAGGATTCCTATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATaaa                                         <  2:319426/140‑1 (MQ=255)
                                                                                                aCTATAAGGATTCCAATTATTCTGTTCTGGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATaaa                                         <  2:1397543/140‑1 (MQ=255)
                                                                                                aCTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATaaa                                         <  2:1502678/140‑1 (MQ=255)
                                                                                                  tataAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATaaa                                                     <  1:2404982/126‑1 (MQ=255)
                                                                                                  tataAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATaaa                                                     >  2:2404982/1‑126 (MQ=255)
                                                                                                  tataAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGa                                       >  1:1665978/1‑140 (MQ=255)
                                                                                                  tataAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGa                                       >  2:1488742/1‑140 (MQ=255)
                                                                                                  tataAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGa                                       >  1:209045/1‑140 (MQ=255)
                                                                                                  tataAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGCTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAAAACGCGGGAATCACGCATGCAGGCATTTATAAAACATATAATAAAGa                                       >  2:1576536/1‑140 (MQ=255)
                                                                                                    taAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAGACATATAATAAAGAAc                                     >  2:691914/1‑140 (MQ=255)
                                                                                                    taAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAAc                                     >  2:12805/1‑140 (MQ=255)
                                                                                                    taAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAAc                                     >  2:1416232/1‑140 (MQ=255)
                                                                                                    taAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAAc                                     >  2:165383/1‑140 (MQ=255)
                                                                                                    taAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTAGGAAACATATAATAAAGAAc                                     >  1:1193577/1‑140 (MQ=255)
                                                                                                    taAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCGGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAAc                                     >  1:1148982/1‑140 (MQ=255)
                                                                                                         aTTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTAt                                >  2:2346585/1‑140 (MQ=255)
                                                                                                          ttCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAGCCCTATGAACAGTACATAAAGAATCATATTTTTAAACCTGCGGGAATGACGCATGCAGGCTTTTACAAAACATATGAAAAAGAACCTTATc                               <  2:317318/140‑1 (MQ=255)
                                                                                                          ttCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAGCCCTATGAACAGTACATAAAGAATCATATTTTTAAACCTGCGGGAATGACGCATGCAGGCTTTTACAAAACATATGAAAAAGAACCTTATc                               <  2:2332302/140‑1 (MQ=255)
                                                                                                          ttCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAGCCCTATGAACAGTACATAAAGAATCATATTTTTAAACCTGCGGGAATGACGCATGCAGGCTTTTACAAAACATATGAAAAAGAACCTTATc                               <  2:1051084/140‑1 (MQ=255)
                                                                                                          ttCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAGCCCTATGAACAGTACATAAAGAATCATATTTTTAAACCTGCGGGAATGACGCATGCAGGCTTTTACAAAACATATGAAAAAGAACCTTATc                               <  2:1716070/140‑1 (MQ=255)
                                                                                                          ttCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAGCCCTATGAACAGTACATAAAGAATCATATTTTTAAACCTGCGGGAATGACGCATGCAGGCTTTTACAAAACATATGAAAAAGAACCTTATc                               <  2:1330049/140‑1 (MQ=255)
                                                                                                          ttCCAACTATTCTGTTCTTGCTTATATTATTGCTGAAGTAAGCGGTGAGCCCTATGAACAGTACATAAAGAATCATATTTTTAAACCTGCGGGAATGACGCATGCAGGCTTTTACAAAACATATGAAAAAGAACCTTATc                               <  2:1206339/140‑1 (MQ=255)
                                                                                                           tCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATcc                              >  1:1835187/1‑140 (MQ=255)
                                                                                                           tCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATcc                              >  1:462394/1‑140 (MQ=255)
                                                                                                           tCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATcc                              >  1:2044667/1‑140 (MQ=255)
                                                                                                            ccAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCct                             >  2:200367/1‑140 (MQ=255)
                                                                                                               attattCTGTTCTCGCTTATACTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATaaa                                         >  2:2005740/1‑125 (MQ=255)
                                                                                                                 tgttCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGt                        <  1:296105/138‑1 (MQ=255)
                                                                                                                 tattCTGTTCTCGCTTATATTGTAGCTGAAGTAGGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGt                        <  2:783151/140‑1 (MQ=255)
                                                                                                                 tattCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGt                        <  2:709962/140‑1 (MQ=255)
                                                                                                                 tattCGGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGt                        <  2:1127276/140‑1 (MQ=255)
                                                                                                                   ttCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCg                      >  1:1635512/1‑140 (MQ=255)
                                                                                                                        ttCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCGGGtat                 <  1:1640818/140‑1 (MQ=255)
                                                                                                                        ttCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCGGGtat                 <  1:1576795/140‑1 (MQ=255)
                                                                                                                         tctcGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCGGGtata                <  1:1154133/140‑1 (MQ=255)
                                                                                                                                   atTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCGGTTATAAGCTGAAggg      <  1:1343348/140‑1 (MQ=255)
                                                                                                                                   atTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCGGGTATAAGCTGAAggg      <  1:12805/140‑1 (MQ=255)
                                                                                                                                   agtGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCGGGTATAAGCTGAAggg      <  2:885851/138‑1 (MQ=255)
                                                                                                                                     tGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCTGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCGGGTATAAGCTGAAGGgca    >  2:1266985/1‑140 (MQ=255)
                                                                                                                                       tAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCGGGTATAAGCTGAAGGgcagc  <  2:462394/140‑1 (MQ=255)
                                                                                                                                       tAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCGGGTATAAGCTGAAGGgcagc  >  1:2008802/1‑140 (MQ=255)
                                                                                                                                       tAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCGGGTATAAGCTGAAGGgcagc  >  2:2066408/1‑140 (MQ=255)
                                                                                                                                       tAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCGGGTATAAGCTGAAGGgcagc  >  2:2075111/1‑140 (MQ=255)
                                                                                                                                       tAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCGGGTATAAGCTGAAGGgcagc  >  2:228105/1‑140 (MQ=255)
                                                                                                                                       tAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCGGGTATAAGCTGAAGGgcagc  >  2:92997/1‑140 (MQ=255)
                                                                                                                                       tAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCGGGTATAAGCTGAAGGgcagc  >  2:93166/1‑140 (MQ=255)
                                                                                                                                       tAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCGGGTATAAGCTGAAGGgcagc  >  2:2266170/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                          
TAAACGGTCATATTGAAGGAAACGGCGCAATCACTCCGGACGATTTAATAAAAGACGTAGAACATCAAGGAATCAAACGTCAGCCGGGTGTATGGGACTATAAGGATTCCAATTATTCTGTTCTCGCTTATATTGTAGCTGAAGTAAGCGGTGAGTCTTATGAACAATACATAACGAATCATATTTTTAAACCGGCGGGAATGACGCATGCAGGCTTTTATAAAACATATAATAAAGAACCTTATCCTGCTGTCGGGTATAAGCTGAAGGGCAGC  >  NZ_CP034943/1734285‑1734559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: