Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,738,217 A→T 5.4% intergenic (+146/‑120) spoVS → / → tdh stage V sporulation protein SpoVS/L‑threonine 3‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,738,2170AT 5.4% 361.6 / 7.3 106intergenic (+146/‑120)spoVS/tdhstage V sporulation protein SpoVS/L‑threonine 3‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (31/69);  new base T (4/2);  total (35/71)
Fisher's exact test for biased strand distribution p-value = 9.07e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

ATAAAGCATTCTCAACCTGTTTGCTTCACGCAAACAGGTTGTTTTTCATTTGTTGTGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGAATGTAATAAGAATTTTTCAAGGAGGATTGTACATGCGGAGTGGA  >  NZ_CP034943/1738078‑1738348
                                                                                                                                            |                                                                                                                                   
aTAAAGCATTCTCAACCTGTTTGCTTCACGCAAACAGGTTGTTTTTCATTTGTTGTGATATTTTCTTCGGATAGTATTTTTAGCATATAAAAGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGaaaaaaa                                                                                                                                     >  1:1844781/1‑140 (MQ=255)
aTAAAGCATTCTCAACCTGTTTGCTTCACGCAAACAGGTTGTTTTTCATTTGTTGTGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACACAAGCCAGAAATGATTTAGAACGTTTCAGaaaaaaa                                                                                                                                     >  1:108086/1‑140 (MQ=255)
aTAAAGCATTCTCAACCTGTTTGCTTCACGCAAACAGGTTGTTTTTCATTTGTTGTGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGaaaaaaa                                                                                                                                     >  1:579601/1‑140 (MQ=255)
aTAAAGCATTCTCAACCTGTTTGCTTCACGCAAACAGGTTGTTTTTCATTTGTTGTGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGaaaaaaa                                                                                                                                     >  1:1091840/1‑140 (MQ=255)
aTAAAGCATTCTCAACCTGTTTGCTTCACGCAAACAGGTTGTTTTTCATTTGTTGTGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGaaaaaaa                                                                                                                                     >  1:2135512/1‑140 (MQ=255)
         tctcAACCTGTTTGCTTCACGCAAACAGGTTGTTTTTCATTTGTTGTGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCa                                                                                                                            >  1:1854873/1‑140 (MQ=255)
              aCCTGTTTGCTTCACGCAAACAGGTTGTTTTTCATTTGTTGTGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCAttttt                                                                                                                       >  1:1252502/1‑140 (MQ=255)
              aCCTGTTTGCTTCACGCAAACAGGTTGTTTTTCATTTGTTGTGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCAttttt                                                                                                                       >  1:1868145/1‑140 (MQ=255)
                     tGCTTCACGCAAACAGGTTGTTTTTCATTTGTTGTGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACa                                                                                                                >  1:970208/1‑140 (MQ=255)
                       cTTCACTCAAACAGGTTGTTTTTCATTTGTTGTTATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATTATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGa                                                                                                              <  2:8256/140‑1 (MQ=255)
                       cTTCACGCAAACATGTTGTTTTTCATTTGTTGTGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGa                                                                                                              <  2:1723284/140‑1 (MQ=255)
                       cTTCACGCAAACAGGTTGTTTTTCATTTGTTGTGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGa                                                                                                              >  1:1590774/1‑140 (MQ=255)
                       cTTCACGCAAACAGGTTGTTTTTCATTTGTTGTGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGa                                                                                                              <  2:1726093/140‑1 (MQ=255)
                       attCACGCAAACAGGTTGTTTTTCATTTGTTGTGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGa                                                                                                              <  2:1245305/139‑1 (MQ=255)
                           atgCAAACAGGTTGTTTTTCATTTCTTGTGATATTTTCTCCCGATAGTATTTTTAGCACATAAATGAGCGAATATACAAAATCAGAAATGAGCTAAAACGTTTCAAAAAAAATTTCCGCCCATTTTTCAAGACAGATACt                                                                                                          >  2:1940144/3‑140 (MQ=255)
                           atgCAAACAGGTTGTTTTTCATTTATTGTGATATTTTCTCCCGATAGTATTTTTAGCACATAAATGAGCGAATATACAAAATCAGAAATGAGCTAAAACGTTTCAAAAAAAATTTCCGCCCATTTTTCAAGACAGATACt                                                                                                          >  2:19681/3‑140 (MQ=255)
                           atgCAAACAGGTTGTTTTTCATTTATTGTGATATTTTCTCCCGATAGTATTTTTAGCACATAAATGAGCGAATATACAAAATCAGAAATGAGCTAAAACGTTTCAAAAAAAATTTCCGCCCATTTTTCAAGACAGATACt                                                                                                          >  2:19512/3‑140 (MQ=255)
                           atgCAAACAGGTTGTTTTTCATTTATTGTGATATTTTCTCCCGATAGTATTTTTAGCACATAAATGAGCGAATATACAAAATCAGAAATGAGCTAAAACGTTTCAAAAAAAATTTCCGCCCATTTTTCAAGACAGATACt                                                                                                          >  2:772199/3‑140 (MQ=255)
                             gCAAACAGGTTGTTTTTCATTTGTTGTGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACttt                                                                                                        >  2:1458846/1‑140 (MQ=255)
                             gCAAACAGGTTGTTTTTCATTTGTTGTGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTAAGAAAAAAATTCAGCCCATTTTTCTAGACAGATACttt                                                                                                        >  2:813602/1‑140 (MQ=255)
                                 aCAGGTTGTTTTTCATTTGTTGTGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTc                                                                                                    <  2:1755742/140‑1 (MQ=255)
                                    gtttgttTTTCATTTGTTGTGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTc                                                                                                 <  2:1526649/138‑1 (MQ=255)
                                    ggTTGTTTTTCATTTTTTGTGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTc                                                                                                 <  1:414181/140‑1 (MQ=255)
                                    ggTTGTTTTTCATTTGTTGTGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTc                                                                                                 <  1:631180/140‑1 (MQ=255)
                                    ggTTGTTTTTCATTTGTTGTGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTc                                                                                                 <  1:1100719/140‑1 (MQ=255)
                                    ggTTGTTTTTCATTTGTTGTGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTc                                                                                                 <  2:1543438/140‑1 (MQ=255)
                                    ggTTGTTTTTCATTTGTTGTGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTc                                                                                                 <  1:1432343/140‑1 (MQ=255)
                                       tgttTTTCATTTGTTGTGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTc                                                                                              >  1:611223/1‑140 (MQ=255)
                                         tttttCATTTGTTGTGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTTAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAg                                                                                            <  1:2404636/140‑1 (MQ=255)
                                         tttttCATTTGTTGTGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTccg                                                                                            <  1:377159/140‑3 (MQ=255)
                                         tttttCATTTGTTGTGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAg                                                                                            <  1:830756/140‑1 (MQ=255)
                                                   gttgtGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTg                                                                                  <  2:1647165/140‑1 (MQ=255)
                                                   gttgtGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTg                                                                                  <  2:347350/140‑1 (MQ=255)
                                                   gttgtGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTg                                                                                  <  2:1118999/140‑1 (MQ=255)
                                                   gttgtGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTg                                                                                  <  2:1071362/140‑1 (MQ=255)
                                                        gATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGtt                                                                             <  1:580047/140‑1 (MQ=255)
                                                        gATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGtt                                                                             <  1:1991349/140‑1 (MQ=255)
                                                        gATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGtt                                                                             <  1:1978216/140‑1 (MQ=255)
                                                        gATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGtt                                                                             <  2:82468/140‑1 (MQ=255)
                                                        gATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTGCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGtt                                                                             <  2:1713907/140‑1 (MQ=255)
                                                        gATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTGTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGtt                                                                             <  1:731400/140‑1 (MQ=255)
                                                            ttttCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACCTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACg                                                                         <  2:32497/140‑1 (MQ=255)
                                                             tttCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGc                                                                        <  2:1123161/140‑1 (MQ=255)
                                                             tttCTCCCGATAGTATTTTTATCACATACATGAGCGAATATACAAAATCAGAAATGAGCTAAAACGTTTCAAAAAAAATTTCCGCCCAGTTTTCAAGACAGATACTTTTGTCCTTCTGGAAAAGACAGTTCTGTTTACGc                                                                        <  2:1919229/140‑1 (MQ=255)
                                                                cttcCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTa                                                                     <  1:1023966/140‑1 (MQ=255)
                                                                  tcCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTAtt                                                                   <  2:1798945/140‑1 (MQ=255)
                                                                  tcCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTAtt                                                                   <  2:1199982/140‑1 (MQ=255)
                                                                  tcCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTAtt                                                                   <  2:1798930/140‑1 (MQ=255)
                                                                  tcCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTAtt                                                                   <  2:505114/140‑1 (MQ=255)
                                                                  tcCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTAtt                                                                   <  2:2348376/140‑1 (MQ=255)
                                                                  tcCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTAtt                                                                   <  2:1951605/140‑1 (MQ=255)
                                                                  tcCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTAtt                                                                   <  2:1592095/140‑1 (MQ=255)
                                                                     gATCGTATTTTTAGCATATAACTGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACGTTTGTCTTCCTCAGAACGACAGTGCTGTTTACGCTTATTccc                                                                <  2:917351/140‑2 (MQ=255)
                                                                     gATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCa                                                                <  1:2094169/140‑1 (MQ=255)
                                                                     gATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCa                                                                <  1:1878362/140‑1 (MQ=255)
                                                                     gATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCa                                                                <  1:314593/140‑1 (MQ=255)
                                                                     gATAGTATTTTTAGCATATAAAGGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCa                                                                <  2:40938/140‑1 (MQ=255)
                                                                          tATTTTTAGCATATCAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCa                                                           <  2:623567/140‑1 (MQ=255)
                                                                          tATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACCGCTACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTAGTCCATATCa                                                           <  2:622756/140‑1 (MQ=255)
                                                                          tATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCa                                                           <  1:879718/140‑1 (MQ=255)
                                                                          tATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCa                                                           <  1:2136967/140‑1 (MQ=255)
                                                                          tATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCa                                                           <  1:761547/140‑1 (MQ=255)
                                                                                    atatAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATc                                                 >  2:1576433/1‑140 (MQ=255)
                                                                                    atatAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATc                                                 >  2:507876/1‑140 (MQ=255)
                                                                                      ataAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCg                                               >  2:1820300/1‑140 (MQ=255)
                                                                                       taAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTg                                                                                  <  2:2076691/104‑1 (MQ=255)
                                                                                       taAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTg                                                                                  <  2:2072226/104‑1 (MQ=255)
                                                                                       taAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTg                                                                                  >  1:2072226/1‑104 (MQ=255)
                                                                                       taAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTg                                                                                  <  2:2076738/104‑1 (MQ=255)
                                                                                       taAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTg                                                                                  >  1:2076738/1‑104 (MQ=255)
                                                                                       taAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGa                                              >  2:1005882/1‑140 (MQ=255)
                                                                                             aCGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGAATGtaa                                        >  2:976886/1‑140 (MQ=255)
                                                                                             aCGGAGATCCAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAACACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTCCAATCCGAATGgaa                                        >  2:1421546/1‑140 (MQ=255)
                                                                                                          aaGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGAATGTAATAAGAATTTTTc                            >  1:617500/1‑140 (MQ=255)
                                                                                                          aaGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGAATGTAATAAGAATTTTTc                            >  2:1284006/1‑140 (MQ=255)
                                                                                                          aaGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGAATGTAATAAGAATTTTTc                            >  1:732382/1‑140 (MQ=255)
                                                                                                             ccAGAAATGATTTATAACGTTTCAGAAAAAATTTCCGCCCATTGTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTTCTGTTTAAGCTTATTCAATATCTTGGTACAAGCCGAATGTAATAAGAATTTTTCAag                         <  1:643382/140‑1 (MQ=255)
                                                                                                             ccAGAAATGATTTATAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGAATGTAATAAGAAtt                                >  1:757232/1‑133 (MQ=255)
                                                                                                             ccAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGAATGTAATAAGAAtt                                <  2:757232/133‑1 (MQ=255)
                                                                                                             ccAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGAATGTAATAAGAAtt                                <  2:756817/133‑1 (MQ=255)
                                                                                                             ccAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGAATGTAATAAGAAtt                                >  1:756817/1‑133 (MQ=255)
                                                                                                             ccAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGAATGTAATAAGAATTTTTCAag                         <  1:779389/140‑1 (MQ=255)
                                                                                                             ccAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGAATGTAATAAGAATTTTTCAag                         <  1:1820300/140‑1 (MQ=255)
                                                                                                             ccAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGAATGTAATAAGAATTTTTCAag                         <  2:617500/140‑1 (MQ=255)
                                                                                                             ccAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGAATGTAATAAGAATTTTTCAag                         >  2:2408678/1‑140 (MQ=255)
                                                                                                             ccAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGAATGTAATAAGAATTTTTCAag                         <  1:431907/140‑1 (MQ=255)
                                                                                                             ccAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATGTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGAATGTAATAAGAATTTTTCAag                         <  1:779237/140‑1 (MQ=255)
                                                                                                             ccAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCAATTTTTCAAGACAGATTCTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGAATGTAATAAGAATTTTTCAag                         <  2:766045/140‑1 (MQ=255)
                                                                                                             acAGAATTGATTTAGAAAGTTTAAGAACAATATTTCGCCCATTTTTCAAGACAGTTACTTTTGTCTTCCTACTAAAGAAAGTGCTTTTTACGCTTATTGCATATCATGGTTCAATCCGAATGTAATAAGAAtttttatag                         <  2:493488/139‑5 (MQ=255)
                                                                                                                 aaaTGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGAATGTAATAAGAATTTTTCAaggagg                     >  1:1828354/1‑140 (MQ=255)
                                                                                                                   aTGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGAATGTAATAAGAATTTTTCAAGGAGGAt                   <  1:293607/140‑1 (MQ=255)
                                                                                                                           gcacgggtCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGAATGTAATAAGAATTTTTCAAGGAGGATTGTACATg           <  1:1263686/133‑1 (MQ=255)
                                                                                                                           gAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGAATGTAATAAGAATTTTTCAAGGAGGATTGTACATg           <  2:1252502/140‑1 (MQ=255)
                                                                                                                           gAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGAATGTAATAAGAATTTTTCAAGGAGGATTGTACATg           <  2:1868145/140‑1 (MQ=255)
                                                                                                                             aCGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGAATGTAATAAGAATTTTTCAAGGAGGATTGTACATGCg         <  2:1590774/140‑1 (MQ=255)
                                                                                                                             aCGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGAATGTAATAAGAATTTTTCAAGGAGGATTGTACATGCg         <  1:376713/140‑1 (MQ=255)
                                                                                                                             aCGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGAATGTAATAAGAATTTTTCAAGGAGGATTGTACATGCg         <  1:1382872/140‑1 (MQ=255)
                                                                                                                             aCGGTTCAGAACAAAATTCCGCCCATTTTTCAAGACAGATACTTTGGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGAATGTAATAAGAATTTTTCAAGGAGGATTGTACATGCg         <  2:2266498/140‑1 (MQ=255)
                                                                                                                                 ttCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGAATGTAATAAGAATTTTTCAAGGAGGATTGTACATGCGGAGt     >  1:448356/1‑140 (MQ=255)
                                                                                                                                    aGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGAATGTAATAAGAATTTTTCAAGGAGGATTGTACATGCGGAGTGGa  <  1:1005882/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                   
ATAAAGCATTCTCAACCTGTTTGCTTCACGCAAACAGGTTGTTTTTCATTTGTTGTGATATTTTCTTCCGATAGTATTTTTAGCATATAAATGACGGATATACAAAAGCCAGAAATGATTTAGAACGTTTCAGAAAAAAATTCCGCCCATTTTTCAAGACAGATACTTTTGTCTTCCTCAGAAAGACAGTGCTGTTTACGCTTATTCCATATCATGGTACAATCCGAATGTAATAAGAATTTTTCAAGGAGGATTGTACATGCGGAGTGGA  >  NZ_CP034943/1738078‑1738348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: