Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,754,574 G→T 5.7% R254I (AGA→ATA) ‡ EO946_RS09090 → acyltransferase domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,754,5740GT 5.7% 368.4 / 8.9 123R254I (AGA→ATA) ‡EO946_RS09090acyltransferase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (49/67);  new base T (3/4);  total (52/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 124 total aligned reads displayed.

AGTGCCTATGCAGAATTTTTAAAATCAATCTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTCGATTATGAC  >  NZ_CP034943/1754441‑1754712
                                                                                                                                     |                                                                                                                                          
aGTGCCTATGCAGAATTTTTAAGATCAAAATCTTTTCAAAAACCGTCAATACCGATTGTATCGAGCTTAACGGGAAGCTGCCTTCACGTAATGGACGAGAATTTCTTTTGGAATGCGGTCAGAAAGCCAATGATGTTTcg                                                                                                                                      >  2:1242392/1‑140 (MQ=255)
    ccTATGCAGAATTTTTAAAATCAATCTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGaa                                                                                                                                  >  2:1277186/1‑140 (MQ=255)
    ccTATGCAGAATTTTTAAAATCAATCTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGaa                                                                                                                                  >  2:451249/1‑140 (MQ=255)
    ccTATGCAGAATTTTTAAAATCAATCTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGaa                                                                                                                                  >  2:398663/1‑140 (MQ=255)
    ccTATGCAGAATTTTTAAAATCAATCTCTTTCAAAAACCCATCAATACCGATTGTATCGAGGTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGAGaa                                                                                                                                  >  2:2101975/1‑140 (MQ=255)
          cAGAATTTTTAAAATCAATCTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGAtt                                                                                                                            <  1:1113479/140‑1 (MQ=255)
          cAGAATTTTTAAAATCAATCTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGAtt                                                                                                                            <  2:944178/140‑1 (MQ=255)
          cAGAATTTTTAAAATCAATCTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTGCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGAtt                                                                                                                            <  2:1828066/140‑1 (MQ=255)
                    aagatcaaaaTCTTTTCAAAAACCGTCAATACCGATTGTATCGAGCTTAACGGGAAGCTGCCTTCACGTAATGGACGAGAATTTCTTTTGGAATGCGGTCAGAAAGCCAATGATGTTTCGTGAAGCGATTCGATATTTgg                                                                                                                  <  1:2241069/137‑1 (MQ=255)
                     aactcaatcTCTTTCAAAAACCCATCAATACCGAGTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGa                                                                                                                 <  2:2072464/137‑1 (MQ=255)
                     aaaTCAATCTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGa                                                                                                                 <  2:372176/140‑1 (MQ=255)
                      aatcactcTCGTGCACACACCCATCAATACCGATTGTAGCGAGTTTACCGGGAAGCTGCCCTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGaa                                                                                                                <  1:1333090/140‑1 (MQ=255)
                      aatcaatcTGTTTCCAAAACCCATCAAGACCGATTGTCTCGAGATTAACCGGAACCTGCCTTCACGAACTGGACACGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGaa                                                                                                                <  1:924904/140‑1 (MQ=255)
                      aatcaatcTCTTTCAACAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGaa                                                                                                                <  1:1072718/140‑1 (MQ=255)
                      aatcaatcTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGaa                                                                                                                <  1:897798/140‑1 (MQ=255)
                      aatcaatcTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGaa                                                                                                                <  1:1436068/140‑1 (MQ=255)
                      aatcaatcTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGaa                                                                                                                <  1:1296626/140‑1 (MQ=255)
                      aatcaatcTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGaa                                                                                                                <  1:897800/140‑1 (MQ=255)
                      aatcaatcTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGaa                                                                                                                <  1:1290081/140‑1 (MQ=255)
                      aatcaatcTCTTTCAAAAACCCATCAATACCGATTGTATAGACTGTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGaa                                                                                                                <  1:1941464/140‑1 (MQ=255)
                             ctctTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGc                                                                                                         >  2:1735336/1‑140 (MQ=255)
                             ctctTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGc                                                                                                         >  2:765089/1‑140 (MQ=255)
                                tttCAAAAACCCATCTATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATa                                                                                                      <  2:2066769/140‑1 (MQ=255)
                                  tCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACg                                                                                                    <  1:934444/140‑1 (MQ=255)
                                  tCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGAATACg                                                                                                    <  1:1536628/140‑1 (MQ=255)
                                     aaaaCCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGc                                                                                                 <  2:304392/140‑1 (MQ=255)
                                     aaaaCCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGc                                                                                                 <  2:674860/140‑1 (MQ=255)
                                     aaaaCCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGc                                                                                                 <  2:1494334/140‑1 (MQ=255)
                                      aaaCCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGcc                                                                                                <  1:715082/140‑1 (MQ=255)
                                      aaaCCAATCAAGACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGAAAAGAATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGcc                                                                                                <  1:2393983/140‑1 (MQ=255)
                                           cATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGtat                                                                                           <  2:1386578/140‑1 (MQ=255)
                                           cATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGtat                                                                                           <  2:1582156/140‑1 (MQ=255)
                                           cATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGtat                                                                                           <  2:219275/140‑1 (MQ=255)
                                             tCAATACCGATTGTATCGAGTTTACCGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTatat                                                                                         <  2:1222505/140‑1 (MQ=255)
                                             tCAATACCGATTGTATCGAGTTTACCGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTatat                                                                                         <  2:1784010/140‑1 (MQ=255)
                                             tCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTatat                                                                                         <  1:39171/140‑1 (MQ=255)
                                             tCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTatat                                                                                         <  1:1532814/140‑1 (MQ=255)
                                             tCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGAATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTatat                                                                                         <  1:2183574/140‑1 (MQ=255)
                                                  aCCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGTATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATAGGTGCCGGTATATCGAtt                                                                                    <  1:56781/140‑1 (MQ=255)
                                                      aTTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAgg                                                                                >  2:745949/1‑140 (MQ=255)
                                                      aTTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAgg                                                                                >  2:1139197/1‑140 (MQ=255)
                                                      aTTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAgg                                                                                >  1:1101358/1‑140 (MQ=255)
                                                      aTTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAgg                                                                                >  1:1294704/1‑140 (MQ=255)
                                                       ttGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGgt                                                                               <  1:1710657/140‑1 (MQ=255)
                                                           aTCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGgtccgt                                                                           >  1:1161335/1‑140 (MQ=255)
                                                           aTCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGgtccgt                                                                           >  1:1788321/1‑140 (MQ=255)
                                                            tCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGgtccgtc                                                                          >  1:1727805/1‑140 (MQ=255)
                                                            tCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGAGATTCGATATTTGGAAAGCCAGCATACGCGCCGGTATATCGATTTAGgtccttc                                                                          >  2:2117419/1‑140 (MQ=255)
                                                               aGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCgg                                                                       <  1:184894/140‑1 (MQ=255)
                                                               aGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCgg                                                                       <  1:1575635/140‑1 (MQ=255)
                                                               aGTTTAACGCGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGACCCCATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCACCATACGTGCCGGTATATCGATTTAGCTCCGTCCgg                                                                       <  1:1519956/140‑1 (MQ=255)
                                                                 tttAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCa                                                                     >  2:315544/1‑140 (MQ=255)
                                                                   tAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCCATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCAcc                                                                   <  1:792426/140‑1 (MQ=255)
                                                                   tAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCAcc                                                                   <  1:780873/140‑1 (MQ=255)
                                                                   tAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCAcc                                                                   <  1:1337244/140‑1 (MQ=255)
                                                                   tAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCAcc                                                                   <  1:959330/140‑1 (MQ=255)
                                                                    aaCGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCt                                                                  >  1:291417/1‑140 (MQ=255)
                                                                                   tCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGa                                                   >  2:832878/1‑140 (MQ=255)
                                                                                     aCGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGaaa                                                 <  1:2115260/140‑1 (MQ=255)
                                                                                          aTGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCt                                            >  2:2053186/1‑140 (MQ=255)
                                                                                          aTGGACAAACATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCt                                            >  2:843221/1‑140 (MQ=255)
                                                                                              aCAAGCATTTCTTGTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTTGGTCCGTCCGGCAGCTTAGCTGCTTTTGTGAAAGAGCTGATc                                        <  2:356912/140‑1 (MQ=255)
                                                                                                 aGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCg                                     >  1:2411119/1‑140 (MQ=255)
                                                                                                 aGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCg                                     >  1:268684/1‑140 (MQ=255)
                                                                                                 aGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCg                                     >  1:1940028/1‑140 (MQ=255)
                                                                                                 aGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCg                                     >  1:590852/1‑140 (MQ=255)
                                                                                                  gCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCgg                                    <  2:683194/140‑1 (MQ=255)
                                                                                                  gCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCgg                                    <  2:232867/140‑1 (MQ=255)
                                                                                                  gCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCgg                                    <  2:2147106/140‑1 (MQ=255)
                                                                                                  gCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCgg                                    <  2:1444863/140‑1 (MQ=255)
                                                                                                   cATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCTGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCggg                                   <  2:1469694/140‑1 (MQ=255)
                                                                                                   cATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTatat                                                                                         >  2:1195336/1‑86 (MQ=255)
                                                                                                       tCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGAt                               >  1:1750857/1‑140 (MQ=255)
                                                                                                       tCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGAt                               >  1:1765028/1‑140 (MQ=255)
                                                                                                                   cTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCgc                   >  2:221937/1‑140 (MQ=255)
                                                                                                                   cTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCgc                   >  2:534042/1‑140 (MQ=255)
                                                                                                                   cTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGCCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCgc                   >  1:1168575/1‑140 (MQ=255)
                                                                                                                         gagaACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCgctgctgc             >  2:575505/1‑140 (MQ=255)
                                                                                                                         gagaACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCgctgctgc             <  1:315544/140‑1 (MQ=255)
                                                                                                                         gagaACCAATGAGATTTCGCGAAGCGATTCGAGATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCgctgctgc             >  2:1224975/1‑140 (MQ=255)
                                                                                                                           gaACCACTGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTc           <  1:2402073/140‑1 (MQ=255)
                                                                                                                           gaACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTGCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTc           <  2:1113137/140‑1 (MQ=255)
                                                                                                                           gaACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTc           <  1:2402047/140‑1 (MQ=255)
                                                                                                                            agccAATGATGTTTCGTGAAGCGATTCGATATTTGGAAAGCCAGCATACATGCAAGTTTATCGATTTAGGGCCATCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATTCCGGGTGATTCAGCTGACCGTTGCTGCTca          <  1:321885/138‑2 (MQ=255)
                                                                                                                            agccAATGATGTTTCGTGAAGCGATTCGATATTTGGAAAGCCAGCATACATGCAAGTTTATCGATTTAGGGCCATCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATTCCGGGTGATTCAGCTGACCGTTGCTGCTca          <  1:2083642/138‑2 (MQ=255)
                                                                                                                            agccAATGATGTTTCGTGAAGCGATTCGATATTTGGAAAGCCAGCATACATGCAAGTTTATCGATTTAGGGCCATCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATTCCGGGTGATTCAGCTGACCGTTGCTGCTca          <  1:1242392/138‑2 (MQ=255)
                                                                                                                             aCCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTCGa         >  2:1611380/1‑140 (MQ=255)
                                                                                                                             aCCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTCGa         >  2:245647/1‑140 (MQ=255)
                                                                                                                             aCCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTCGa         >  2:2246228/1‑140 (MQ=255)
                                                                                                                              ccAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTCGAt        >  2:2148533/1‑140 (MQ=255)
                                                                                                                              ccAATGAGATGTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCTATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGGTCCCGGGAGATTCAGCGAACCGCTGCTGCTCGAt        >  2:57532/1‑140 (MQ=255)
                                                                                                                                aaTGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGc                                                                                                 >  2:870210/1‑49 (MQ=255)
                                                                                                                                aaTGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGc                                                                                                 <  1:870210/49‑1 (MQ=255)
                                                                                                                                aaTGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTCGATTa      >  2:2222624/1‑140 (MQ=255)
                                                                                                                                aaTGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTCGATTa      >  2:2222407/1‑140 (MQ=255)
                                                                                                                                 aTGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTCGATTAt     <  1:1423832/140‑1 (MQ=255)
                                                                                                                                 aTGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTCGATTAt     <  1:2080208/140‑1 (MQ=255)
                                                                                                                                  tGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCgctg                 >  2:56925/1‑127 (MQ=255)
                                                                                                                                  tGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCgctg                 <  1:56925/127‑1 (MQ=255)
                                                                                                                                    agaTTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGCCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTCGATTATGac  <  2:1168575/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                          
AGTGCCTATGCAGAATTTTTAAAATCAATCTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTCGATTATGAC  >  NZ_CP034943/1754441‑1754712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: