Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,754,575 A→G 5.7% R254R (AGA→AGG) ‡ EO946_RS09090 → acyltransferase domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,754,5750AG 5.7% 396.6 / 8.7 123R254R (AGA→AGG) ‡EO946_RS09090acyltransferase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (49/67);  new base G (3/4);  total (52/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

AGTGCCTATGCAGAATTTTTAAAATCAATCTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTCGATTATGACAC  >  NZ_CP034943/1754441‑1754714
                                                                                                                                      |                                                                                                                                           
aGTGCCTATGCAGAATTTTTAAGATCAAAATCTTTTCAAAAACCGTCAATACCGATTGTATCGAGCTTAACGGGAAGCTGCCTTCACGTAATGGACGAGAATTTCTTTTGGAATGCGGTCAGAAAGCCAATGATGTTTcg                                                                                                                                        >  2:1242392/1‑140 (MQ=255)
aGTGCCTATGCAGAATTTTTAAGATCAAAATCTTTTCAAAAACCGTCAATACCGATTGTATCGAGCTTAACGGGAAGCTGCCTTCACGTAATGGACGAGAATTTCTTTTGGAATGCGGTCAGAAAGCCAATGATGTTTcg                                                                                                                                        >  2:321885/1‑140 (MQ=255)
    ccTATGGAGAATTTTTAAAATCAATCGCTGTCAAAAACCCATCAATACCGCTTGTATCGCGTGTAACGGGACGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGCACCAATGAGATTTcgcgca                                                                                                                                    >  2:654815/1‑138 (MQ=255)
    ccTATGCAGAATTTTTAAAATCAATCTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGaa                                                                                                                                    >  2:1277186/1‑140 (MQ=255)
    ccTATGCAGAATTTTTAAAATCAATCTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGaa                                                                                                                                    >  2:451249/1‑140 (MQ=255)
    ccTATGCAGAATTTTTAAAATCAATCTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGaa                                                                                                                                    >  2:398663/1‑140 (MQ=255)
    ccTATGCAGAATTTTTAAAATCAATCTCTTTCAAAAACCCATCAATACCGATTGTATCGAGGTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGAGaa                                                                                                                                    >  2:2101975/1‑140 (MQ=255)
        tGCAGAATTTTTAAAATCAATCTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGa                                                                                                                                >  1:1299212/1‑140 (MQ=255)
          cAGAATTTTTAAAATCAATCTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGAtt                                                                                                                              <  1:200934/140‑1 (MQ=255)
          cAGAATTTTTAAAATCAATCTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGAtt                                                                                                                              <  1:1113479/140‑1 (MQ=255)
          cAGAATTTTTAAAATCAATCTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGAtt                                                                                                                              <  2:944178/140‑1 (MQ=255)
          cAGAATTTTTAAAATCAATCTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTGCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGAtt                                                                                                                              <  2:1828066/140‑1 (MQ=255)
                     aactcaatcTCTTTCAAAAACCCATCAATACCGAGTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGa                                                                                                                   <  2:2072464/137‑1 (MQ=255)
                      aatcactcTCGTGCACACACCCATCAATACCGATTGTAGCGAGTTTACCGGGAAGCTGCCCTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGaa                                                                                                                  <  1:1333090/140‑1 (MQ=255)
                      aatcaatcTCTTTCAACAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGaa                                                                                                                  <  1:1072718/140‑1 (MQ=255)
                      aatcaatcTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGaa                                                                                                                  <  1:1290081/140‑1 (MQ=255)
                      aatcaatcTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGaa                                                                                                                  <  1:1436068/140‑1 (MQ=255)
                      aatcaatcTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGaa                                                                                                                  <  1:897800/140‑1 (MQ=255)
                      aatcaatcTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGaa                                                                                                                  <  1:897798/140‑1 (MQ=255)
                      aatcaatcTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGaa                                                                                                                  <  1:607703/140‑1 (MQ=255)
                      aatcaatcTCTTTCAAAAACCCATCAATACCGATTGTATAGACTGTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGaa                                                                                                                  <  1:1941464/140‑1 (MQ=255)
                             ctctTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGc                                                                                                           >  2:765089/1‑140 (MQ=255)
                             ctctTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGc                                                                                                           >  2:1735336/1‑140 (MQ=255)
                                tttCAAAAACCCATCTATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATa                                                                                                        <  2:2066769/140‑1 (MQ=255)
                                  tCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACg                                                                                                      <  1:934444/140‑1 (MQ=255)
                                  tCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGAATACg                                                                                                      <  1:2414882/140‑1 (MQ=255)
                                     aaaaCCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGc                                                                                                   <  2:1494334/140‑1 (MQ=255)
                                      aaaCCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGcc                                                                                                  <  1:715082/140‑1 (MQ=255)
                                      aaaCCAATCAAGACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGAAAAGAATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGcc                                                                                                  <  1:2393983/140‑1 (MQ=255)
                                           cATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGtat                                                                                             <  2:218687/140‑1 (MQ=255)
                                           cATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGtat                                                                                             <  2:219275/140‑1 (MQ=255)
                                             tCAATACCGATTGTATCGAGTTTACCGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTatat                                                                                           <  2:1784010/140‑1 (MQ=255)
                                             tCAATACCGATTGTATCGAGTTTACCGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTatat                                                                                           <  2:1222505/140‑1 (MQ=255)
                                             tCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTatat                                                                                           <  1:1532814/140‑1 (MQ=255)
                                             tCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTatat                                                                                           <  1:2265058/140‑1 (MQ=255)
                                             tCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTatat                                                                                           <  1:877138/140‑1 (MQ=255)
                                             tCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTatat                                                                                           <  1:39171/140‑1 (MQ=255)
                                             tCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGAATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTatat                                                                                           <  1:2183574/140‑1 (MQ=255)
                                                  aCCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGTATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATAGGTGCCGGTATATCGAtt                                                                                      <  1:56781/140‑1 (MQ=255)
                                                      aTTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAgg                                                                                  >  1:1101358/1‑140 (MQ=255)
                                                      aTTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAgg                                                                                  >  1:1294704/1‑140 (MQ=255)
                                                      aTTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAgg                                                                                  >  2:745949/1‑140 (MQ=255)
                                                       ttGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGgt                                                                                 <  1:1710657/140‑1 (MQ=255)
                                                           aTCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGgtccgt                                                                             >  1:1161335/1‑140 (MQ=255)
                                                           aTCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGgtccgt                                                                             >  1:1788321/1‑140 (MQ=255)
                                                            tCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGAGATTCGATATTTGGAAAGCCAGCATACGCGCCGGTATATCGATTTAGgtccttc                                                                            >  2:2117419/1‑140 (MQ=255)
                                                               aGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCgg                                                                         <  1:1575635/140‑1 (MQ=255)
                                                               aGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCgg                                                                         <  1:1517100/140‑1 (MQ=255)
                                                               aGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCgg                                                                         <  1:1773165/140‑1 (MQ=255)
                                                               aGTTTAACGCGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGACCCCATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCACCATACGTGCCGGTATATCGATTTAGCTCCGTCCgg                                                                         <  1:1519956/140‑1 (MQ=255)
                                                                 tttAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCa                                                                       >  2:315544/1‑140 (MQ=255)
                                                                   tAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCCATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCAcc                                                                     <  1:792426/140‑1 (MQ=255)
                                                                   tAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCAcc                                                                     <  1:959330/140‑1 (MQ=255)
                                                                   tAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCAcc                                                                     <  1:780873/140‑1 (MQ=255)
                                                                   tAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCAcc                                                                     <  1:1337244/140‑1 (MQ=255)
                                                                   gaaCGGGCAGCCGCCTTCACGAAAAGGAGAAGCAATTCTTTTGGAATGCTGTCAGAGACCCAATGAGATTTCGCGACGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCAcc                                                                     <  1:951468/139‑1 (MQ=255)
                                                                    aaCGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCt                                                                    >  1:291417/1‑140 (MQ=255)
                                                                                     aCGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGaaa                                                   <  1:2115260/140‑1 (MQ=255)
                                                                                          aTGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCt                                              >  2:2053186/1‑140 (MQ=255)
                                                                                          aTGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCt                                              >  2:1510367/1‑140 (MQ=255)
                                                                                          aTGGACAAACATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCt                                              >  2:843221/1‑140 (MQ=255)
                                                                                              aCAAGCATTTCTTGTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTTGGTCCGTCCGGCAGCTTAGCTGCTTTTGTGAAAGAGCTGATc                                          <  2:356912/140‑1 (MQ=255)
                                                                                                 aGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCg                                       >  1:1940028/1‑140 (MQ=255)
                                                                                                 aGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCg                                       >  1:268684/1‑140 (MQ=255)
                                                                                                  gCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCgg                                      <  2:1444863/140‑1 (MQ=255)
                                                                                                  gCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCgg                                      <  2:683194/140‑1 (MQ=255)
                                                                                                  gCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCgg                                      <  2:2147106/140‑1 (MQ=255)
                                                                                                  gCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCgg                                      <  2:232867/140‑1 (MQ=255)
                                                                                                   cATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCTGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCggg                                     <  2:1469694/140‑1 (MQ=255)
                                                                                                   cATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTatat                                                                                           >  2:1195336/1‑86 (MQ=255)
                                                                                                   cATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTatat                                                                                           <  1:1195336/86‑1 (MQ=255)
                                                                                                      ttCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGgaga                                  <  1:1687893/140‑1 (MQ=255)
                                                                                                      ttCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGgaga                                  <  1:36534/140‑1 (MQ=255)
                                                                                                       tCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGAt                                 >  1:1750857/1‑140 (MQ=255)
                                                                                                       tCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGAt                                 >  1:1765028/1‑140 (MQ=255)
                                                                                                                   cTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCgc                     >  2:534042/1‑140 (MQ=255)
                                                                                                                   cTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCgc                     >  2:221937/1‑140 (MQ=255)
                                                                                                                         gagaACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTTAAACAGCTGATCCCGGGAGAATCAGCGAACAgctgctgc               >  2:2309446/1‑140 (MQ=255)
                                                                                                                         gagaACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCgctgctgc               >  2:120511/1‑140 (MQ=255)
                                                                                                                         gagaACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCgctgctgc               >  2:575505/1‑140 (MQ=255)
                                                                                                                           gaACCACTGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTc             <  1:2402073/140‑1 (MQ=255)
                                                                                                                           gaACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTGCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTc             <  2:1113137/140‑1 (MQ=255)
                                                                                                                           gaACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTc             <  1:2402047/140‑1 (MQ=255)
                                                                                                                            agccAATGATGTTTCGTGAAGCGATTCGATATTTGGAAAGCCAGCATACATGCAAGTTTATCGATTTAGGGCCATCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATTCCGGGTGATTCAGCTGACCGTTGCTGCTca            <  1:1242392/138‑2 (MQ=255)
                                                                                                                            agccAATGATGTTTCGTGAAGCGATTCGATATTTGGAAAGCCAGCATACATGCAAGTTTATCGATTTAGGGCCATCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATTCCGGGTGATTCAGCTGACCGTTGCTGCTca            <  1:321885/138‑2 (MQ=255)
                                                                                                                            agccAATGATGTTTCGTGAAGCGATTCGATATTTGGAAAGCCAGCATACATGCAAGTTTATCGATTTAGGGCCATCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATTCCGGGTGATTCAGCTGACCGTTGCTGCTca            <  1:2083642/138‑2 (MQ=255)
                                                                                                                             aCCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTCGa           >  2:1611380/1‑140 (MQ=255)
                                                                                                                             aCCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTCGa           >  2:245647/1‑140 (MQ=255)
                                                                                                                             aCCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTCGa           >  2:2246228/1‑140 (MQ=255)
                                                                                                                              ccAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTCGAt          >  2:1358541/1‑140 (MQ=255)
                                                                                                                              ccAATGAGATTTCGCGAAGCGATTCGATATGTGGAAAGCCAGCATACGTGCCGGTATATCGATATAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCGCGGGAGATTCAGCGTACCGCTGCTGCTCGAt          >  2:514676/1‑140 (MQ=255)
                                                                                                                              ccAATGAGATGTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCTATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGGTCCCGGGAGATTCAGCGAACCGCTGCTGCTCGAt          >  2:57532/1‑140 (MQ=255)
                                                                                                                                aaTGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGc                                                                                                   >  2:870210/1‑49 (MQ=255)
                                                                                                                                aaTGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTCGATTa        >  2:2222407/1‑140 (MQ=255)
                                                                                                                                aaTGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTCGATTa        >  2:766610/1‑140 (MQ=255)
                                                                                                                                 aTGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTCGATTAt       <  1:2080208/140‑1 (MQ=255)
                                                                                                                                  tGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCgctg                   <  1:56925/127‑1 (MQ=255)
                                                                                                                                    agaTTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGCCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTCGATTATGac    <  2:1168575/140‑1 (MQ=255)
                                                                                                                                      aTTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTCGATTATGacac  >  1:37499/1‑140 (MQ=255)
                                                                                                                                      aTTTCGCGAAGCGATTCGAGATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTCGATTATGacac  >  1:407820/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                           
AGTGCCTATGCAGAATTTTTAAAATCAATCTCTTTCAAAAACCCATCAATACCGATTGTATCGAGTTTAACGGGAAGCTGCCTTCACGAAATGGACAAGCATTTCTTTTGGAATGCTGTCAGAGAACCAATGAGATTTCGCGAAGCGATTCGATATTTGGAAAGCCAGCATACGTGCCGGTATATCGATTTAGGTCCGTCCGGCACCTTAGCTGCTTTTGTGAAACAGCTGATCCCGGGAGATTCAGCGAACCGCTGCTGCTCGATTATGACAC  >  NZ_CP034943/1754441‑1754714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: